Cobblestone, 77 King Street North, Dublin. Bars & pubs - Time Out Dublin. Loft Market, Powerscourt Townhouse Centre, Dublin. Shops - Time Out Dublin. Music - Search results - Time Out Dublin. 651 results, sorted alphabetically Music The Point Theatre, Dublin's largest indoor music venue, closed its doors in the summer of 2007 to undergo a complete overhaul and rebranding as The O2,... 101 Talbot.
20 great things to do in Dublin - Time Out Dublin. By Time Out editors and Sulakshana Gupta 1.
If your browser does not accept cookies, you cannot view this site. Setting Your Browser to Accept Cookies There are many reasons why a cookie could not be set correctly. Three-dimensional structure of aspartyl protease from human immunodeficiency virus HIV-1. Three-dimensional structure of aspartyl protease from human immunodeficiency virus HIV-1 Manuel A.
Science Magazine: Sign In. PIN domain. In molecular biology the PIN domain is a protein domain that is about 130 amino acids in length.
The PIN domain was named after its identification in the N-terminus of the PilT protein (PilT N terminus). PIN domains function as nuclease enzymes that cleave single stranded RNA in a sequence dependent manner. PIN domains contain three nearly invariant acidic residues. Crystal structures show these residues clustered together in the putative active site. Celebrating macromolecular crystallography: A personal perspective. From crystallography to structural biology, a century of discoveries. Directory of Open Access Journals.
Celebrating macromolecular crystallography: A personal perspectiveAbad-Zapatero, CelerinoArbor: Ciencia, Pensamiento y Cultura. 2015;191(772):a215 DOI 10.3989/arbor.2015.772n2001Abstract | Full Text The twentieth century has seen an enormous advance in the knowledge of the atomic structures that surround us.
The discovery of the first crystal structures of simple inorganic salts by the Braggs in 1914, using the diffraction of X-rays by crystals, provided the critical elements to unveil the atomic structure of matter. Subsequent developments in the field leading to macromolecular crystallography are presented with a personal perspective, related to the cultural milieu of Spain in the late 1950’s. A VapBC Toxin-Antitoxin Module Is a Posttranscriptional Regulator of Metabolic Flux in Mycobacteria.
+ Author Affiliations The largest family of toxin-antitoxin (TA) modules are encoded by the vapBC operons, but their roles in bacterial physiology remain enigmatic.
Microarray analysis in Mycobacterium smegmatis overexpressing VapC/VapBC revealed a high percentage of downregulated genes with annotated roles in carbon transport and metabolism, suggesting that VapC was targeting specific metabolic mRNA transcripts. To validate this hypothesis, purified VapC was used to identify the RNA cleavage site in vitro. VapC had RNase activity that was sequence specific, cleaving single-stranded RNA substrates at AUAU and AUAA in vitro and in vivo (viz., MSMEG_2121 to MSMEG_2124). A bioinformatic analysis of these regions suggested that an RNA hairpin 3′ of the AUA(U/A) motif is also required for efficient cleavage. The Biochemistry of VapBC Toxin-Antitoxins. Shigella flexneri. Shigella flexneri is a species of Gram-negative bacteria in the genus Shigella that can cause diarrhea in humans.
Several different serogroups of Shigella are described; S. flexneri belongs to group B. S. flexneri infections can usually be treated with antibiotics, although some strains have become resistant. Less severe cases are not usually treated because they become more resistant in the future. Discovery The species was named after the American physician Simon Flexner; the genus is named for Japanese physician Kiyoshi Shiga, who researched the cause of dysentery.
VapBC. First discovered in 1992, vapBC loci are now thought make up around 37–42% of all type II toxin-antitoxin systems. Discovery Distribution VapBC operons have been found in distantly related prokaryotes, including the pathogens Leptospira interrogans, Mycobacterium tuberculosis and Piscirickettsia salmonis. The loci have been described as "surprisingly abundant, especially in Archaea"—vapBC family members made up 37% of all TA families identified by one bioinformatics search and 42% of those found by another. Bioinformatics searches have discovered vapBC homologues on both chromosomes and plasmids, and often in high copy number per cell.