Sperm-RNA-Marks-Male-Fertility.html?utm_source=dlvr. Researchers-Report-First-Mechanism-Compound.html?utm_source=dlvr. Discovery of the first molecular mechanism of action for a small molecule that improves RNA splicing in cells could lead to new therapeutics for the genetic disorder spinal muscular atrophy (SMA) and perhaps other conditions involving improper RNA processing.
After cells transcribe genomic DNA into RNA, an RNA-protein complex called the spliceosome removes noncoding sequences, or introns, from RNA transcripts, enabling the coding sequences, or exons, to be translated into functional proteins. Currently, no approved drugs are specifically designed to address errors in RNA splicing. SMA is the leading genetic cause of infant mortality, with more than half of patients dying before age two. It is caused by the absence of the gene SMN1, which encodes SMN, a protein that protects motor neurons. There is a duplicate gene called SMN2, but it cannot fully make up for the resulting SMN deficiency because its RNA transcript is often spliced incorrectly.
Susanne E. Adrian R. New-Insecticidal-Strategy-Kills-Crop.html?utm_source=dlvr. The Colorado potato beetle costs the agricultural industry billions of dollars per year and devours so many crops around the world that the insect has been branded an “international super pest.”
Because the pest has become resistant to all major classes of insecticides and has few natural enemies, crop scientists are seeking a strategy to rein in the beetle’s feeding frenzies. A team of researchers led by Ralph Bock at the Max Planck Institute for Molecular Plant Physiology, in Potsdam, Germany, now reports that it has found a way to protect crops from the Colorado potato beetle with a new insecticidal tool: RNA interference, or RNAi (Science 2015, DOI: 10.1126/science.1261680). To use RNAi against the pest, the researchers first identified a gene the insect can’t do without—one that encodes a cytoskeleton protein vital to maintaining a cell’s shape. Login. Insights into Domain–Domain Motions in Proteins and RNA from Solution NMR - Accounts of Chemical Research. Design, Synthesis, and Structure–Activity Relationships of Pyridoquinazolinecarboxamides as RNA Polymerase I Inhibitors - Journal of Medicinal Chemistry. Self-Assembling-Nanoparticles-Sneak-Antisense.html?utm_source=dlvr.
Since the 1980s, researchers have pursued antisense nucleic acids as a way to reduce the expression of disease-causing genes.
But delivering the sequences into a cell and protecting them from degradation have proved challenging. Using a new type of nanoparticle, a California team has successfully delivered antisense RNA into a cell and used it to shut down the expression of a cancer-related gene (J. Am. Chem. Soc. 2014, DOI: 10.1021/ja503598z). To build antisense nucleic acid strands, researchers design a short stretch of DNA or RNA complementary to a messenger RNA target. To facilitate delivery and avoid degradation, researchers have looked to nanoparticles to help escort sequences inside cells. The antisense sequence targets the mRNA of survivin, a protein implicated in several types of cancer. To make a self-assembling nanoparticle, the researchers attached the antisense LNA to a norbornyl polymer. Chad A. In Silico Design and Enzymatic Synthesis of Functional RNA Nanoparticles - Accounts of Chemical Research. † Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, Maryland 21702, United States ‡ Basic Science Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland 21702, United States § Gene Regulation and Chromosome Biology Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, Maryland 21702, United States.
The Predictive Power of Synthetic Nucleic Acid Technologies in RNA Biology - Accounts of Chemical Research. National Centre for Biological Sciences, TIFR, GKVK Bellary Road, Bangalore, 560065 Karnataka, India Acc.
Chem. Res., 2014, 47 (6), pp 1710–1719 DOI: 10.1021/ar400323d Publication Date (Web): April 8, 2014 Copyright © 2014 American Chemical Society Biography Saikat Chakraborty received his Bachelors in Chemistry from Presidency College, Kolkata, in 2003 and M.Sc. in Biochemistry from University of Calcutta in 2005. Combination of Dendrimer-Nanovector-Mediated Small Interfering RNA Delivery to Target Akt with the Clinical Anticancer Drug Paclitaxel for Effective and Potent Anticancer Activity in Treating Ovarian Cancer - Journal of Medicinal Chemistry (ACS Publicatio. † School of Biological Sciences, University of Hong Kong, Pokfulam Road, Pokfulam, Hong Kong ‡ Aix-Marseille University, CNRS, Centre Interdisciplinaire de Nanoscience de Marseille, CINaM UMR 7325, Marseille Cedex 09, France § Molecular Simulation Engineering Laboratory (MOSE), Department of Engineering and Architecture (DEA), University of Trieste, Via Valerio 10, 34127 Trieste, Italy Research Unit MOSE-DEA, National Interuniversity Consortium for Material Science and Technology (INSTM), University of Trieste, Via Valerio 6, 34127 Trieste, Italy J.
Med. High-Affinity Recognition of HIV-1 Frameshift-Stimulating RNA Alters Frameshifting in Vitro and Interferes with HIV-1 Infectivity - Journal of Medicinal Chemistry. †Departments of Chemistry, ‡Biochemistry and Biophysics, and §Dermatology, University of Rochester, Rochester, New York 14642, United States Oyagen, Inc., Henrietta, New York 14623, United States J.
Med. Chem., 2014, 57 (3), pp 723–732 DOI: 10.1021/jm401438g Publication Date (Web): January 5, 2014 Copyright © 2014 American Chemical Society. Single Molecule Fluorescence Approaches Shed Light on Intracellular RNAs - Chemical Reviews. Unlocked Nucleic Acids Help Silence Genes Selectively. Login. November 18, 2013 Issue - Vol. 91 Issue 46. Discovery of Novel Bacterial RNA Polymerase Inhibitors: Pharmacophore-Based Virtual Screening and Hit Optimization - Journal of Medicinal Chemistry. †Helmholtz-Institute for Pharmaceutical Research Saarland, Department of Drug Design and Optimization, and ‡Pharmaceutical and Medicinal Chemistry, Saarland University, Campus C2.3, 66123 Saarbrücken, Germany J.
Med. Login. Nanobiology of RNA Polymerase: Biological Consequence of Inhomogeneity in Reactant - Chemical Reviews. Faculty of Life Sciences, Kyoto Sangyo University, Kamigamo-Motoyama, Kita-Ku, Kyoto, 603-8555 Japan Chem.
Rev., 2013, 113 (11), pp 8400–8422 DOI: 10.1021/cr400006b Publication Date (Web): September 27, 2013 Copyright © 2013 American Chemical Society Section: 1. Citing Articles View all 1 citing articles Citation data is made available by participants in CrossRef's Cited-by Linking service. Single-Molecule Studies of RNA Polymerases - Chemical Reviews. † Biophysics Institute, Faculty of Natural Sciences, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany ‡ Center for Integrated Protein Science Munich (CIPSM), Department of Chemistry and Biochemistry, Munich University, Butenandtstrasse 5-13, 81377 München, Germany § Department of Bioengineering, Stanford University, James H.
Selective Inhibitors of Bacterial t-RNA-(N1G37) Methyltransferase (TrmD) That Demonstrate Novel Ordering of the Lid Domain - Journal of Medicinal Chemistry. Pamela J. Hill *†, Ayome Abibi ‡, Robert Albert †, Beth Andrews ‡, Moriah M. Gagnon †, Ning Gao ‡, Tyler Grebe †, Laurel I. Hajec ‡, Jian Huang ‡, Stephania Livchak ‡, Sushmita D. RNA Polymerase II C-Terminal Domain: Tethering Transcription to Transcript and Template - Chemical Reviews. Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore Maryland 21205, United States Chem. Rev., 2013, 113 (11), pp 8423–8455 DOI: 10.1021/cr400158h Publication Date (Web): September 16, 2013 Copyright © 2013 American Chemical Society Section: 1. Citing Articles View all 4 citing articles Citation data is made available by participants in CrossRef's Cited-by Linking service.
This article has been cited by 1 ACS Journal articles (1 most recent appear below). New Compound Stops Flu Virus’s Thievery. In hopes of adding new weapons to the antiflu arsenal, researchers have set their sights on an enzyme that helps the virus replicate in host cells by stealing RNA. The team now reports a new class of molecules that inhibit this enzyme and prevent influenza from replicating in mammalian cells (ACS Chem. Biol. 2013, DOI: 10.1021/cb400400j). The enzyme, called polymerase acidic protein (PA), is a part of the virus’ polymerase complex. Login. Computational Simulation Strategies for Analysis of Multisubunit RNA Polymerases - Chemical Reviews. The RNA Polymerase Factory and Archaeal Transcription - Chemical Reviews. The RNA Polymerase II Carboxy-Terminal Domain Code - Chemical Reviews. Role of RNA Polymerase and Transcription in the Organization of the Bacterial Nucleoid - Chemical Reviews.
August 12, 2013 Issue - Vol. 91 Issue 32. RNA Polymerase II Transcription Elongation Control - Chemical Reviews. The Writers, Readers, and Functions of the RNA Polymerase II C-Terminal Domain Code - Chemical Reviews. Ancient Rocks Suggest Meteorites Brought Life’s Phosphorus To Earth. X-ray Crystal Structures of the Escherichia coli RNA Polymerase in Complex with Benzoxazinorifamycins - Journal of Medicinal Chemistry.
Discovery of a Potent Boronic Acid Derived Inhibitor of the HCV RNA-Dependent RNA Polymerase - Journal of Medicinal Chemistry. Andrew Maynard ‡ , Renae M. Bandages Silence Genes. Medical researchers think specially tailored RNA sequences could turn off genes in patients’ cells to encourage wound healing or to kill tumor cells. Now researchers have developed a nanocoating for bandages that could deliver these fragile gene-silencing RNAs right where they’re needed ( ACS Nano 2013, DOI: 10.1021/nn401011n ). The team hopes to produce a bandage that shuts down genes standing in the way of healing in chronic wounds.
Small interfering RNAs, or siRNAs, derail expression of specific genes in cells by binding to other RNA molecules that contain the code for those genes. Biologists have developed siRNAs that target disease-related genes. But for these siRNAs to reach the clinic, researchers must find a way to deliver the molecules safely to the right cells. Other groups have tackled this delivery challenge by attaching siRNAs to chemical carriers that protect the oligonucleotides as they travel through the bloodstream. Login. Login. Stacked RNA Base Analogs Could Be A Clue To Life’s Beginnings. Detecting RNA Based On Color. Login. October 15, 2012 Issue - Vol. 90 Issue 42. RNA G-Quadruplex Tagged Selectively. Login. Login. Probe Detects Single Copy Of Viral RNA.
A new single-stranded DNA probe lights up when it binds just one copy of viral RNA inside a cell (Anal. Chem., DOI: 10.1021/ac3023873). Such a probe could help biologists study the progression of viral infections in cells and understand how drugs disrupt the infections, say the researchers who designed it. The new method uses fluorescence in situ hybridization (FISH), a common technique for identifying nucleic acids inside cells or tissue. In FISH, scientists add chemicals to cells to freeze biomolecules in place.
Then they apply to the cells a nucleic acid probe marked with a radioactive or fluorescent label. But the long DNA probes used in FISH often bind to other nucleic acids in the cell, limiting its detection ability. Principles for Understanding the Accuracy of SHAPE-Directed RNA Structure Modeling - Biochemistry. Login. Chemical & Engineering News: Protein Factory Reveals Its Secrets. An Error Occurred Setting Your User Cookie. Login. Nanoparticle Conjugates Mimic RNA Interference. Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA... RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes...
Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries... RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes. RNA Gets Special Treatment To Remove It From Blood. Login. Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA. Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries. A Peptide Nucleic Acid–Aminosugar Conjugate Targeting Transactivation Response Element of HIV-1 RNA Genome Shows a High Bioavailability in Human Cells and Strongly Inhibits Tat-Mediated Transactivation of HIV-1 Transcription - Journal of Medicinal Chemist.
Supersensitive Detection Of MicroRNAs. Intramolecular Free-Radical Cyclization Reactions on Pentose Sugars for the Synthesis of Carba-LNA and Carba-ENA and the Application of Their Modified Oligonucleotides as Potential RNA Targeted Therapeutics - Chemical Reviews. Login.