Fluorescence Microscopy | Ultraviolet Filter Combinations | UV-2A. Fluorescence Filter Combinations Ultraviolet Excitation: UV-2A (Longpass Emission) The Nikon UV-2A ultraviolet fluorescence filter combination includes a medium-width bandpass excitation filter (330-380 nanometers) coupled to a longpass barrier filter (420 nanometer cut-on wavelength) to enable collection of signals from a wide spectrum of fluorescent probes. Ultraviolet and visible transmission spectral profiles for this filter combination are illustrated below in Figure 1. The common dichromatic mirror for the Nikon UV-2 series of filter combinations has a cut-on wavelength of 400 nanometers and reflects greater than 95 percent of the ultraviolet wavelengths between 330 and 390 nanometers. Ultraviolet Excitation Filter Block UV-2A Specifications: Excitation Filter Wavelengths: 330-380 nanometers (bandpass, 355 CWL) Dichromatic Mirror Cut-on Wavelength: 400 nanometers (longpass, LP) Barrier Filter Wavelengths: 420 nanometer cut-on (longpass, LP)
Critical thinking web. The Internet Classics Archive: 441 searchable works of classical literature. Introduction to Microscopy. Introduction to Optical Microscopy,Digital Imaging, and Photomicrography This treatise on optical microscopy is divided into several sections that are available through the links displayed immediately to the left (in the darker navigational boxes) and below.
In order to print the entire microscopy primer as a paper document, you must download each link independently, send the file to your printer, and put the results together. In the Bibliography, we have included links to other works on optical microscopy and our section on Web Resources contains links to other microscopy sites on the Internet. This material is targeted for educational purposes only, and is not available to be posted on remote websites (either commercial or educational) or distributed in any electronic format. Frequently Asked Questions - Mortimer Abramowitz, senior microscopist at Olympus America Inc., answers the 50 most commonly asked questions about microscopy and photomicrography. Questions or comments? How to Exercise Your Brain for Better Thinking Skills.
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