Scientists propose revolutionary DNA-based approach to map the wiring diagram of the brain. A team of neuroscientists have proposed a new and potentially revolutionary way of determining the neuronal connectivity (the “connectome”) of the whole brain of the mouse, in an essay published October 23 in the open access journal PLOS Biology.
The team, led by Professor Anthony Zador, Ph.D. of Cold Spring Harbor Laboratory, aims to provide a comprehensive account of neural connectivity. At present the only method for obtaining this information with high precision relies on examining individual cell-to-cell contacts (synapses) by electron microscopy. But such methods are slow, expensive and labor-intensive. Zador and colleagues instead propose to exploit high-throughput DNA sequencing to probe the connectivity of neural circuits at the resolution of single neurons.
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