New Collagen School Stuff. Collagen project. China.....Woking conditions etc.. APA Formatting and Style Guide. Note: This page reflects the latest version of the APA Publication Manual (i.e., APA 7), which released in October 2019.
The equivalent resource for the older APA 6 style can be found here. Please use the example at the bottom of this page to cite the Purdue OWL in APA. Immunofluorescence Labeling Method. The purpose of immunofluorescence is to detect the location and relative abundance of any protein for which you have an antibody.
Once you have antibodies to your favorite protein, you can use them to indicate where the protein is located. In this example, we will use antibodies for the calcium ATPase, or pump, that is located in the endoplasmic reticulum (ER) of very cell. The antibody used here only recognized the chicken calcium ATPase but immunofluorescence can be used on any protein. The key to this entire process is the ability to visualize the antibody when looking through a microscope. 08002_03aug09_ihc_guidebook_5th_edition_chapter_10.pdf. iPaint - An online painting program.