Quality Assurance and Improvement — RNA-Seq Course v1.0 documentation. # comments: # What is a k-mer, k-mer size, pathological graphics. # Show why they are that way in the reads using less.
Read Quality Issues Read quality issues come about when “noise” enters a sequence between extraction from the organism and final sequencing output. Many read quality issues are sequencing platform dependent, so it’s very important to adopt read quality processing to specific platforms. Two primary sources of noise exist: SAMtools. Burrows-Wheeler Aligner.