In Silico Protein-protein Interactions Prediction - Profacgen. Protein Mutagenesis - Profacgen. Precise mutagenesis of a target protein is very useful in the study of protein-protein interaction, protein structure, biological activity site etc.
Utilizing the most advanced DNA synthesis technology, Profacgen provides one-stop service on Site-Directed Protein Mutagenesis Service. Single or multiple mutations (substitution, insertion or deletion) can be introduced into your target protein with high accuracy and efficiency. We can also create protein truncation according to your specific requirements. Advantages High accuracy confirmed by full sequencing Large insert size achievable Cost-effective Short turnaround time. Protein Mutant Library Construction - Profacgen.
Protein mutant libraries containing different protein variants are becoming more and more popular in the determination of vital residues controlling physiological activities and pathological process.
Novel components of signal pathways or receptors can be identified through screening of protein mutant libraries. Profacgen's high-throughput gene synthesis, mutagenesis and protein production platforms enable us to speed up your project by offering protein mutant library construction service which can deliver thousands of protein variants efficiently. Our protein mutant library construction service consists of gene synthesis, mutagenesis, sub-cloning, pilot scale protein expression using the E. coli system, and final protein purification.
We use three methods for the gene variant library construction: Protein/Peptide Crosslinking - Profacgen. Crosslinking is a technique that covalently binds two or more biomolecules together to form conjugates with enhanced physical properties and/or biological functionalities.
The structure of a functioning protein involves more than just the intramolecular covalent bonds, which results in challenging questions regarding the spatial orientation and non-covalent interactions of the protein. The dynamic nature of proteins in physiological environment makes it even difficult to determine the roles of individual amino acids in the chemical and biological properties of proteins. PEGylation Products and Services - Profacgen. Introducing polyethylene glycol (PEG) to biomolecules and pharmaceuticals is known to enhance stability and pharmacological properties of proteins, peptides, antibody-drug conjugates and small-molecule drugs.
As a synthetic polymer with high hydrophilicity, PEG is widely used to prolong circulation time of proteins, increase aqueous solubility of therapeutic molecules and reduce immunogenicity of biopharmaceuticals. To date, PEGylation has been successfully used in 12 marketed drugs.1 The PEGylation technology, first used in protein modification, has been recently transferred to small-molecule drugs, leading to a new wave of investigation in pharmaceutical industry.
The two major types of PEG reagents, linear PEG and branched PEG, are both used in marketed drugs. With years of experience in bioconjugation services, Profacgen provides a full spectrum of quality solutions, from single reagents to comprehensive PEGylation service, to meet your specific needs. Protein Biotinylation Service - Profacgen. Profacgen has been providing protein biotinylation service for years and has performed custom biotinylation service in a variety of scales for both academic and industrial customers.
We can perform biotnylation for any protein required. Biotinylated proteins can be used in ELISA assay, proteolysis test, immunoprecipitation assay, immunohistochemistry, cross-linking, flow cytometry, signal transduction experiments, receptor binding studies, and experimental tissue therapy and tissue engineering. Profacgen has developed multiple methods for in vitro biotinylation. You can choose the method that best serves your requirements. Our in vitro protein biotinylation platform offers simple and efficient biotin labeling without altering the bioactivity of target proteins.
Primary amine biotinylation Primary amine biotinylation is the most frequently used protein biotinylation method. Quantum Dot Labeling. Protein Biotinylation Service - Profacgen. Co-Immunoprecipitation (Co-IP) Co-IP is a classic technology widely used for protein-protein interaction identification and validation.
Based on the specific immunological interaction between the bait protein and its antibody, co-IP has become an effective and reliable method in detecting the physiological interaction between proteins. The principle of the co-IP technique is as follows: Many intracellular protein-protein interactions are retained when cells are lysed under non-denaturing conditions. Membrane-Based Yeast Two-Hybrid Screening. The classical Yeast two-hybrid (Y2H) screening is mostly limited to the study of cytosolic or extracellular soluble proteins.
To study the interaction of transmembrane protein interaction, Profacgen provides a specialized membrane-based Y2H (MbY2H) system. Advantages: Specialized for membrane proteins Customized cDNA library construction Experienced team with over ten years of protein interaction study experience Short turnaround time Best price in the market The MbY2H system is also called the split ubiquitin Y2H, whereas your bait protein will be fused to the C-terminal half of ubiquitin (Cub) and a transcription factor; and the transmembrane bait protein library is fused to the N-terminal half (Nub). Protein Interaction Analysis Services. Protein-protein interactions (PPIs) are established when protein molecules physically contact with each other through hydrogen or hydrophobic bonds, Van der Waals forces, ionic forces, or even covalent bonds.
This phenomenon underlies a wide range of essential cellular events such as signal transduction, molecular transport and various metabolism pathways. PPIs are also the basic of many diseases. Therefore, PPIs have been studied extensively in the area of bioscience and medical research. Profacgen has organized a team of scientists with extensive experience in the study of PPIs to offer you one-stop service on your PPI project. We can help with your experiment design, carry out your experiments, and perform data analysis.
Please see the list of assays that we provide below: Custom Lysate Preparation Service. Profacgen provides custom lysate preparation service to meet your scientific requirements for downstream analysis.
We provide various types of lysates including over-expression lysate, tissue lysate, cell line lysate, stem cell lysate and membrane lysate, reaching to more than 15, 000 kinds of lysate available. We actually have many lysate products already available in stock from human, cynomolgus monkey, bovine, canine, ferret, mouse, rat, rabbit, zebrafish, guinea pig and so on. Our high-quality cell/tissue lysate can be used in SDS-PAGE, western blot, ELISA, co-immunoprecipitation, pull down assay, tissue specific expression identification and many other bio-analytical assays, and can be applied in various research fields such as biological and pharmaceutical studies. Profacgen has established an advanced platform for custom lysate preparation with high efficiency and state-of-the-art technology. We can help you select reliable and research-ready lysates. Stable Cell Line Construction for Protein Expression. When large amount of recombinant protein is required, stable expression line construction is recommended for reproducible and cost-effective protein production.
Construction of a stable cell line usually takes 3 steps: delivery of the target gene into the host cell, screening of stable expression clones, and stability test. Features: High yield and reproducibility High cell line stability One-stop service Service fully customizable At Profacgen, we use lentiviral vector for efficient gene delivery and to achieve high integration rate. Limited dilution and antibiotics screening are then applied to select for high-yield, genetically homogenous stable expression monoclones.
PEGylation Products and Services. Protein Labeling. Site-specific labeling technique refers to the covalent attachment of molecules like biotin, fluorophore, enzyme and radioactive isotopes to specific sites of the target protein. Protein labeling is extensively used to enable the detection and purification of the desired protein, and to facilitate the tracking of protein expression, localization, interaction, activity and conformational changes in complicated cellular signaling events. Features Comprehensive list of labeling reagents Site-specific labeling Cost-effective Short turn-around time Profacgen has developed a series of protein labeling techniques and reagents to meet your research requirements.
The most popular labels our customers choose include fluorescent probes (Phycoerythrin, allophycocyanin, fluorescein, tetramethylrhodamine, fluor dyes), enzyme conjugates (horseradish peroxidase and alkaline phosphatase) and biotin. Click here to contact us for more technical information. Nicotiana Tabacum Expression System. Compared to conventional expression systems, such as microbial fermentation and mammalian cell cultures, plant production systems are inexpensive, easily scalable, free from human pathogens and offer the potential for direct oral administration. Tobacco has the most established history for the production of recombinant proteins because it is readily amendable to genetic engineering and has a high biomass yield. Furthermore, the tobacco expression platform is based on leaves, which removes the need for flowering, significantly reducing the potential for gene leakage into the environment through pollen or seed dispersal.
Most importantly, tobacco is a nonfood, nonfeed crop, which minimizes regulatory barriers by eliminating the risk of plant-made recombinant proteins entering the food chain. Profacgen has developed a novel Nicotiana tabacum specific expression platform for recombinant protein production at high purity. Mammalian Cell Expression. Mammalian cell expression systems are the best choice for the production of eukaryotic proteins, especially when correct folding and post-translational modification (glycosylation, phosphorylation, etc.) is required.
They produce eukaryotic recombinant proteins in the most natural state, with native tertiary structure, physiochemical characteristics, and bioactivities. They have been successfully applied in the biopharmaceutical production of cytokines, monoclonal antibodies, growth factors and so on. Yeast Expression. Yeast is the simplest eukaryotic expression system. It sustains post-translational modification and proper folding of eukaryotic proteins, while still allows easy culture and scale-up production at relatively low cost. The two most commonly used yeast species are Saccharomyces cerevisiae and Pichia pastoris. Features. Yeast Two-Hybrid Screening. Specific interactions between proteins form the basis of most biological processes, thus the knowledge of an organism’s protein interaction network provides insights into the function(s) of individual proteins, the structure of functional complexes, and eventually, the organization of the whole cell.
Protein Interaction Analysis Services. Quantum Mechanics/Chemistry in Drug Design. The process of drug discovery involves the identification of molecular candidates, synthesis and test of chemical compounds for their pharmaceutical efficacy. Computer-Aided Drug Design (CADD) is a specialized sub-discipline of rational drug design that uses computational methods to investigate/predict drug-receptor interactions. Quantum mechanics (QM) is an essential tool in CADD research. High-throughput in silico screening of ligand binding (such as docking or QSAR) can significant reduce the time required for compound discovery and optimization. Protein–Carbohydrate Docking. Protein–Peptide Docking.
Protein–Nucleic Acid Docking. ADME/Tox Prediction. The characterization of Absorption, Distribution, Metabolism, and Excretion (also known as ADME) and Toxicity are essential steps in drug development. ADME describes the disposition of a pharmaceutical compound within an organism, and has been applied in drug discovery to investigate the drug levels and kinetics of drug exposure to the tissues and hence influences the performance and pharmacological activity of the compound as a drug. It covers a lot of different assays investigating parameters such as membrane permeability, metabolic stability and toxicity. Quantum Mechanics/Chemistry in Drug Design. Yeast Two-Hybrid Screening. Protein Interaction Analysis Services.
Quantum Mechanics/Chemistry in Drug Design. Protein–Carbohydrate Docking. Protein–Peptide Docking. Protein–Nucleic Acid Docking. ADME/Tox Prediction. Quantum Mechanics/Chemistry in Drug Design. Protein–Nucleic Acid Docking. ADME/Tox Prediction. Quantum Mechanics/Chemistry in Drug Design. Membrane Protein Modeling. Membrane proteins constitute one third of all genes in the human genome, and they play critical roles in cells as receptors, enzymes, ion channels, and transporters. Owing to their functional importance, membrane proteins account for more than 50% of current drug targets. However, experimental structural data are sparse, mostly due to difficulties in their overexpression, reconstitution into membrane mimetics, and subsequent structure determination. The lack of high-resolution structures hinders drug design process and the engineering of receptors with novel function for biotechnological applications, resulting in high expectations for computational modelling tools to help fill this gap.
Computational structural modeling represents an important alternative approach to obtaining three-dimensional membrane protein models at the atomic level. Protein/Peptide Crosslinking. Immunogenicity Testing Services. Immunogenicity testing is a crucial part of biopharmaceutical development. Biosimilar Testing Services. A biosimilar (also known as follow-on biologic or subsequent entry biologic) is a biologic medical product.
Biosimilars are officially approved versions of original "innovator" products, and can be manufactured when the original product's patent expires. Cell Medium Development and QC Tests. Profacgen is committed to providing cell medium customization and cell culture development services for your experimental and production needs. With solid knowledge of technology, we provide high quality culture medium development services, as well as fast and reliable QC tests. Cell Medium Development and QC Tests. Antimicrobial Peptides Services. Antimicrobial Peptides Services. Anaerobic Fermentation. Profacgen, Perfect Protein. PEGylation Products and Services.
PEGylation Products and Services. Protein Biotinylation Service. Protein Biotinylation Service. Quantum Dot Labeling. Protein Biotinylation Service. PEGylation Products and Services. Protein Conjugation Service on Beads. Protein Conjugation Service on Beads. Protein Labeling. NovEgg Expression System. Protein Co-Expression Service in Mammalian Cells. Protein Co-Expression Service in E. coli System. Nicotiana Tabacum Expression System. Nicotiana Tabacum Expression System. Animal-free Expression.
Cell-Free Protein Expression. Mammalian Cell Expression. Co-Immunoprecipitation (Co-IP) CLIP-Seq Service. Chromatin Immunoprecipitation (ChIP) Assay Service. Cell Line Development for Industrial Manufacturing. Cell-free protein expression. Cell-based Kinase Assays. Biosimilar Testing Services. Biopharmaceutical Process and Product Related Impurities Analysis. Platelet derived endothelial cell growth factor. Multiferon. Zoster immune globulin. Antihemophilic factor human. Interferon alfa n1. Dornase alfa mechanism of action. Factor viii molecular weightlogo.
Immunogenicity Test Service. Hydrophobic Tag Services. Homology Modeling. GPCR Screening Service. GLP Compliant Protein Production. Fusion Protein Modeling. Functional Complement Assays. Fold Recognition. Recombinant Human CD38 Protein (His tag), Human Proteins. Recombinant protein expression in Pichia pastoris. Technical Reference Guide for recombinant protein expression in E.coli. Recombinant Cynomolgus CD33 Protein(His tag), Cynomolgus Proteins. Quantum Dot Labeling. Protein Labeling. Protein Conjugation Service on Beads. Protein Co-expression Service in Mammalian Cells. Pull Down Assay. Protein-fluorophore Bioconjugation.