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Recent major advances in DNA sequencing technologies have resulted in next-gen sequencing platforms capable of generating massive amounts of reads very quickly and relatively inexpensively. The new technologies are being effectively utilized for systematic study of genetic variation in certain targeted regions (subsets) of complex genomes 1 . By targeting specific regions, one can take better advantage of next-gen sequencing capability; essentially, more coverage is achieved by focusing reads on your area of interest.
MY baits is a fully customizable liquid-phase DNA capture system for targeted sequencing. We have developed a proprietary technology to synthesize large long-oligonucleotide libraries on solid substrates. We have taken the best of the oligonucleotide synthesis chemistry (high yield, low cost phosphoramidite chemistry) and combined it with digital photolithography for maximum flexibility in parallel synthesis. By so doing, we can synthesize tens of thousands of different oligonucleotides in parallel on the same substrate. The main advantage of combining phosphoramidite chemistry, digital photolithography and liquid phase synthesis is oligonucleotide library synthesis at a reduced cost while maintaining a stepwise yield well above 99%. Oligonucleotides are cleaved off the substrate and converted into biotinylated RNA baits ready to capture your targeted sequences.