TCP Inc. – Knock In, Knock Out & Conditional Knockout Mice. Mouse Reproduction - Transgenic Mice Breeding & Generation. Our breeding facilities are located in Canada’s largest mouse facility. The building is approximately 11,000 gross square metres of space on four occupied and two service floors. It houses specialized laboratories for mouse generation and analyses with holding space and services for up to 36,000 cages (180,000 mice) on individually vented micro isolator cage racks.
The facility’s Exclusion Barrier holding rooms are specifically designed, built, and equipped to provide an optimal and high-security disease-free environment for essential breeding colonies of mice that are accessed and maintained exclusively by Facility Operations staff. Modified Barrier rooms hold animals that have been bred or derived within the Exclusion Barrier and are being actively utilized in research projects. They will also hold mice derived in other locations that have been tested and confirmed pathogen-free.
Back To Services. TCP Inc. – Knock In, Knock Out & Conditional Knockout Mice. Mouse Reproduction — Transgenic Mice: An Overview. Mice Reproduction: About Genetically Engineered Mouse Models. A genetically modified mouse is a model that has its genome altered by making use of various genetic engineering techniques.
Commonly used as animal models or for research of human diseases, genetically engineered mouse models are developed with more precision model human phenotypes and pathologies. This gives researchers chance to explore mechanisms and greater confidence in the translational potential of their findings. Also, many models of neurodegenerative, oncology, cardiovascular disease and metabolic disorders are available. Genetically Engineered Mouse Models are transgenic animals that have made possible to faithfully mimic the genetic and biological evolution of human cancers.
These models offer many advantages over xenograft models and are engineered to have a short latency yet with high penetrance. Conditional Knockout Mouse Models – Medium. Conditional gene knockout is a process or a technique of gene targeting used to eradicate a specific gene in a certain tissue.
This process is extremely helpful in studying the importance of particular genes in living organisms. This method is different from traditional gene knockout as it aims at targeting the specific genes at specific times, rather than deleting them from the beginning of life. However, using conditional gene knockout technique is highly helpful as it eradicates side effects from traditional gene knockout technique. Embryonic death from a gene mutation can happen in traditional gene knockout and it can stop scientists from studying the gene in adults. Some genes must be inactive in certain tissues and cannot be studied properly in isolation. Conditional knockout mice technology represents a valuable research tool, which tells about 15% of gene knockouts are developmentally lethal.
Genetically Modified Mice, Transgenic Mouse Models. TCP Inc. – Knock In, Knock Out & Conditional Knockout Mice. Mouse Reproduction - Transgenic Mice Breeding & Generation. Mutant Mouse and Mouse Phenotyping Service. We have developed, validated, and implemented screening tools for in-depth analysis of the major physiological and neurological systems in the mouse.
Our extensive experience and expertise with state-of-the-art equipment has allowed us to cultivate and provide a broad range of physiological and neurobehaviour assays that are available to our clients. Applications of our assays include characterization of mutant mice for gene effect, drug target effect, and drug compound effect(s) to produce biologically relevant data in early drug discovery. Services Portfolio Summary Neurobehaviour Screens Panel of Primary Screens 1) Combined SHIRPA and Dysmorphology (basic sensory function & general health) 2) Open field (state anxiety/emotionality) 3) Rotarod (motor function) 4) PrePulse Inhibition & Acoustic Startle Reflex (schizophrenia) 5) Tail Suspension (depression) 6) Fear conditioning (learning and memory) Additional Neurobehaviour Screens Motor Function Trait Anxiety Depression-like Behaviour Nociception.
Mouse Breeding & Rederivation Services. Our breeding facilities are located in Canada’s largest mouse facility.
The building is approximately 11,000 gross square metres of space on four occupied and two service floors. It houses specialized laboratories for mouse generation and analyses with holding space and services for up to 36,000 cages (180,000 mice) on individually vented micro isolator cage racks. The facility’s Exclusion Barrier holding rooms are specifically designed, built, and equipped to provide an optimal and high-security disease-free environment for essential breeding colonies of mice that are accessed and maintained exclusively by Facility Operations staff.
Modified Barrier rooms hold animals that have been bred or derived within the Exclusion Barrier and are being actively utilized in research projects. They will also hold mice derived in other locations that have been tested and confirmed pathogen-free. Back To Services.