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FTP, SFTP, WebDAV, Cloud Files, Google Docs & Amazon S3 Browser for Mac & Windows. Seq Anal Each gene has a specific sequence of nucleotides, commonly called its DNA sequence. Once we have cloned a gene into a plasmid we can determine the sequence of the DNA using the dideoxynucleotide method either manually or by using automated sequencers. To seqeunce DNA, the DNA is first denatured, producing a single stranded template. A specific primer is then added which binds to the template. One then subjects the gel to autoradiography (manual technique) or looks for fluorescent dyes (automated technique) to determine the location of each band. One of the cornerstones to the rapid advances in molecular biology and genome research is the ability to rapidly deal with very large DNA sequences using sophisticated programs and powerful computers. Click on either sequencing gel to the right to learn more about the steps involved in DNA sequencing.

Home Page SignUp Stack Rapid Ideas creates the most innovative and stylish rapidweaver themes and stacks for the web design tool RapidWeaver from RealMac Software. If you own a Mac and like to publish your own web site, the combination of those themes and stacks and RapidWeaver, will give you the flexibility and professionality you need. We started our project and business back in 2005 and we always tried to go one step further than other developers for RapidWeaver. Even our main audience are from the creative sector (including photographers, designers, publishers and media worker) our themes and stacks also play well for any website project you may have. Rapid Ideas is a project of Christoph Richardet, the head behind the Company Richardet Design. Since 2005 RapidIdeas always raised the top bar in professional RapidWeaver Theme development. Many customers are working in the design, art or photography area, looking for stylish and clean designs to present their work.

PCR 1) Add the following to a microfuge tube: 10 ul reaction buffer 1 ul 15 uM forward primer 1 ul 15 uM reverse primer 1 ul template DNA 5 ul 2 mM dNTP 8 ul 25 mM MgCl2 or MgSO4 (volume variable) water (to make up to 100 ul) 2) Place tube in a thermocycler. 3) Start the PCR cycles according the following schemes: a) denaturation - 94 ° C, 30-90 sec. b) annealing - 55 °C (or -5° Tm), 0.5-2 min. c) extension - 72 °C, 1 min. repeat cycles 29 times 4) Add a final extension step of 5 min. to fill in any uncompleted polymerisation. Note: Most of the parameters can be varied to optimise the PCR (more at Tavi's PCR guide ): a) Mg ++ - one of the main variables - change the amount added if the PCR result is poor. b) Template DNA concentration - PCR is very powerful tool for DNA amplification therefore very little DNA is needed. c) Enzymes used - Taq DNA polymerase has a higher error rate (no proof-reading 3' to 5' exonuclease activity) than Tli or Pfu . h) Additives - i) PCR buffer 16.6 mM ammonium sulfate

Free Craigslist HTML Code Creator Simple CMS for Web Designers Tutorial - BLAST - Workbench "The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families" (quoted from the NCBI BLAST homepage). geWorkbench submits BLAST jobs to the NCBI server. NCBI-supported sequence databases and search algorithms can be selected in the user interface. Since release 2.1.0, geWorkbench supports almost all BLAST setting options available through the NCBI web interface. Please note that although, in geWorkbench, we have adopted the default settings for each BLAST algorithm as seen on the NCBI website, those settings are subject to change at any time by NCBI. The BLAST analysis is available when a protein or DNA sequence is loaded and selected in the Workspace. Older help pages... Prerequisites

Scripts - Mailing List manager (MLM) Maxprog Mailing List Manager (MLM for short) is a double opt-in list subscription manager and a message opening/click-through tracking PHP script you can install on your web site and start using in minutes. MLM supports a full set of commands you can use either directly with your web browser or with MaxBulk Mailer. Unlike other products and depending on your possibilities and knowledge, MLM is prepared to work using either a mySQL database or simple raw text files. MLM has no limitations, you can create as many lists as you wish with as many subscribers as you need and track the delivery of unlimited messages. For medium to big lists and better security we recommend to use the mySQL option. You can access the recipient lists managed by MLM with MaxBulk Mailer (v6 or higher) selecting 'Remote Lists' from the list manager pull-down menu located on the 'Recipients' panel. Commands All MLM commands can be issued through a Web Browser or through a one-click link on your email messages.

Code Collector - View Snippet CodeCollector.net Login or Sign-up PlusKit PHP Contact Form Solution Send Snippet To: -- view all seydoggy's snippets language: PHPlicense: Other Share on facebookShare on twitterShare on emailShare on printMore Sharing Services0 Code for Snippet: Please enable JavaScript to view the <a href=" Info Tags: php pluskit contact form Link to this snippet: To use the direct link to your snippet on CodeCollector.net either copy the html from the above section or drag the to where you would like to use it. More Info: Drag and drop search for Go Daddy websites Wouldn't it be nice to just drag and drop a Custom Search box onto a website? We thought so, and so did Go Daddy, the world's largest domain name registrar and top web hosting provider. Website owners using Go Daddy's WebSite Tonight product can now easily drag a search widget onto their web pages, and instantly turn on high-quality website search powered by the Custom Search platform. WebSite Tonight is a do-it-yourself service that lets users create, design, update and publish websites without requiring any knowledge of HTML. Here’s how you add Custom Search to your website in WebSite Tonight: Step 1: Select "Google Custom Search" from the list of available widgets Step 2: Select a predefined search theme to match the style of your website Step 3: Drag and drop the Google Custom Search box to the desired location on your website Step 4: Search! Go Daddy also integrates with Google Webmaster Tools as part of the Google Services for Websites program.

50 plugins para manejo multimedia en Wordpress La web se esta convirtiendo cada vez más en un medio multimedia, la incorporación de video, audio e imagen en blogs y web ya es cotidiano. Pero estos medios requieren también de herramientas para que su uso, manejo y distribución se faciliten. Por suerte, para WordPress han salido una gran cantidad de plugins para el manejo de estos elementos y en mashable han hecho una recopilación de los 50 más populares que te podrían ayudar en la incorporación de galerías, efectos, videos, podcast y audio en general. Aquí te presentamos una versión en nuestro idioma. Aniga Gallery – Te permite crear galerías de imágenes que usan el sistema de comentarios. Batch Image Uploader – Sube y dimensiona varias imágenes en una sola vez. Duh Gallery – Crea una galería a partir de los imágenes de tu directorio de uploads. ImageManager – Incluye una interfaz subir y explorar tus imágenes con herramientas para cortarlas o rotarlas. WP Image Gallery – Crea fotogalerías dentro de entradas o páginas de tu blog.

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Con este sencillo script podrás insertar en tu sitio un contador de caracteres que permita al usuario controlar la cantidad utilizada en sus textos. Por ejemplo puede ser utilizado en una aplicación de twitter o en sistemas de auto gestión de contenidos CMS donde se requiera que los gestores de contenido no excedan de cierta cantidad de caracteres. by elpanamoises Aug 3

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