FTP, SFTP, WebDAV, Cloud Files, Google Docs & Amazon S3 Browser for Mac & Windows. Seq Anal Each gene has a specific sequence of nucleotides, commonly called its DNA sequence. Once we have cloned a gene into a plasmid we can determine the sequence of the DNA using the dideoxynucleotide method either manually or by using automated sequencers. To seqeunce DNA, the DNA is first denatured, producing a single stranded template. A specific primer is then added which binds to the template. One then subjects the gel to autoradiography (manual technique) or looks for fluorescent dyes (automated technique) to determine the location of each band. One of the cornerstones to the rapid advances in molecular biology and genome research is the ability to rapidly deal with very large DNA sequences using sophisticated programs and powerful computers. Click on either sequencing gel to the right to learn more about the steps involved in DNA sequencing.
Google Powered Site Search with jQuery Martin Angelov By far one of the most requested features by Tutorialzine’s readers, is building a site-wide search. One way to do it, is to build it yourself from the ground up. Another way is to use the services of the one search engine that already knows everything about everyone. Important update (October 2014): Google has canceled the API that this search engine uses, so this script will no longer work. The HTML Lets start with the HTML markup. search.html <! In the body section, we have the main container element – the #page div. Inside the form is the text input box, after which comes the radio group for searching on the current site / the web, and the four search type icons, organized as an unordered list. Google Powered Site Search The CSS The CSS styles reside in styles.css. styles.css – Part 1 As mentioned above, the form’s functions are not limited to only submitting data, but also to act as a regular container element. styles.css – Part 2 styles.css – Part 3 The jQuery Conclusion
SignUp Stack Rapid Ideas creates the most innovative and stylish rapidweaver themes and stacks for the web design tool RapidWeaver from RealMac Software. If you own a Mac and like to publish your own web site, the combination of those themes and stacks and RapidWeaver, will give you the flexibility and professionality you need. We started our project and business back in 2005 and we always tried to go one step further than other developers for RapidWeaver. Even our main audience are from the creative sector (including photographers, designers, publishers and media worker) our themes and stacks also play well for any website project you may have. Rapid Ideas is a project of Christoph Richardet, the head behind the Company Richardet Design. Since 2005 RapidIdeas always raised the top bar in professional RapidWeaver Theme development. Many customers are working in the design, art or photography area, looking for stylish and clean designs to present their work.
PCR 1) Add the following to a microfuge tube: 10 ul reaction buffer 1 ul 15 uM forward primer 1 ul 15 uM reverse primer 1 ul template DNA 5 ul 2 mM dNTP 8 ul 25 mM MgCl2 or MgSO4 (volume variable) water (to make up to 100 ul) 2) Place tube in a thermocycler. 3) Start the PCR cycles according the following schemes: a) denaturation - 94 ° C, 30-90 sec. b) annealing - 55 °C (or -5° Tm), 0.5-2 min. c) extension - 72 °C, 1 min. repeat cycles 29 times 4) Add a final extension step of 5 min. to fill in any uncompleted polymerisation. Note: Most of the parameters can be varied to optimise the PCR (more at Tavi's PCR guide ): a) Mg ++ - one of the main variables - change the amount added if the PCR result is poor. b) Template DNA concentration - PCR is very powerful tool for DNA amplification therefore very little DNA is needed. c) Enzymes used - Taq DNA polymerase has a higher error rate (no proof-reading 3' to 5' exonuclease activity) than Tli or Pfu . h) Additives - i) PCR buffer 16.6 mM ammonium sulfate
jPreLoader | A Preloading Screen to Preload Images jPreLoader | A Preloading Screen to Preload Images Plugin updated: New version of jPreLoader launched with new features like auto exit, one time preload and some bugs fixed. See change log for more details. Howdy, all! Today I would like to share with you a brand new jQuery plugin, called jPreLoader. jPreLoader can create a preloading screen for your website before all your images (including the images in CSS) are fully loaded. It will come in handy when you wish to preload all the images on your page before exposed to user. Well, you can also create a Splash Screen during preloading process to introduce your products, or describe about your page, or show the instructions of gameplay as well as put an advertisement during the waiting time of your user. jPreLoader works well with jQuery 1.7.1 (others haven’t tested yet) and compatible with IE7 and above, Chrome, Firefox and Safari. This idea is inspired by Gaya’s cool “QueryLoader2” plugin which able to preload your website in style. 1. 2.
Simple CMS for Web Designers Tutorial - BLAST - Workbench "The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families" (quoted from the NCBI BLAST homepage). geWorkbench submits BLAST jobs to the NCBI server. NCBI-supported sequence databases and search algorithms can be selected in the user interface. Since release 2.1.0, geWorkbench supports almost all BLAST setting options available through the NCBI web interface. Please note that although, in geWorkbench, we have adopted the default settings for each BLAST algorithm as seen on the NCBI website, those settings are subject to change at any time by NCBI. The BLAST analysis is available when a protein or DNA sequence is loaded and selected in the Workspace. Older help pages... Prerequisites
13 Best jQuery 360 Degree Image Rotation Plugins Using JQuery 360 Degree Image Rotation Plugin is the most beautiful and convenient way to display the pictures on the web pages. These best 360° pictures allow the user to have a much detailed analysis of the product or article. jQuery Image Rotation Plugins let you display images in any angle 360 degrees, which can be useful for an e-commerce site to help customers look products in detail, from any angle they desire. In this article we have gathered 13 Best jQuery 360 Degree Image Rotation Plugins for our audience, following plugins are capable of displaying images in 360 degree angle. Hope you will choose and use the following 360 degree jQuery plugins according to your needs for your web projects. If you like the article you might be interested in our other article as well on 15 Best jQuery Image Slider Plugins 1. Reel is an established and the most versatile three-sixty player for jQuery. 2. Spritespin is a jQuery plugin that enables sprite animation in your website. 3. 6. 8. 9. 11.