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Maq Featured RNA-Seq Job – Computational Biologist The Opportunity: The Morgridge Institute for Research seeks a Computational Biologist to provide bioinformatics support to researchers studying gene expression, epigenomics, proteomics and transcriptional regulation in embryonic stem cells and their differentiated derivatives. This position is part of the bioinformatics team within stem cell pioneer James Thomson’s Regenerative Biology Laboratory of the Morgridge Institute for Research. The following are core responsibilities of this position: Candidate Requirements: We are seeking candidates who have a master’s degree (or a bachelor’s degree plus equivalent experience and education) in computer science, mathematics, bioinformatics, or biological sciences, Ph.D is preferred. The Organization: The Morgridge Institute for Research benefits the University of Wisconsin–Madison and the citizens of Wisconsin by advancing breakthrough biomedical discoveries to solve important global health challenges and by educating the public about science.

Regex Tutorial—From Regex 101 to Advanced Regex services:dbfetch | EBI Web Services WSDbfetch allows you to retrieve entries from various up-to-date biological databases using entry identifiers or accession numbers. This is equivalent to the CGI based dbfetch service. Like the CGI service a request can return a maximum of 200 entries. For more information see: Web services registry entries: Sample clients are provided for a number of programming languages. There are three interfaces to the WSDbfetch ( SOAP ) service, each with its own WSDL : Fetch a set of entries in a defined format and style. db : the name of the database to obtain the entries from. query : a string containing a comma separated list of entry identifiers. format : the name of the format required. style : the name of the style required. The format of the response depends on the interface to the service used: WSDBFetchServerService and WSDBFetchDoclitServerService: the entries as a string. WSDBFetchServerLegacyService: an array of strings containing the entries. Fetch an entry in a defined format and style.

Master thesis – Bioinformatics – RNA-Seq analysis Development of a RNA-seq analysis pipeline for bacterial transcriptomics Introduction/framework ** Recent developments in new high-throughput technologies have revolutionized molecular biology. This technological progress has led to an explosive growth of the biological information (e.g. via DNA sequencing, RNA microarrays, proteomics), creating new opportunities in the field of bioinformatics in order to computationally deal with the dramatic increase of data. More specifically, the recent availability of next-generation sequencing (NGS) methods has opened up new horizons at the level of gene expression analysis. However, these high-throughput sequencing methods produce a massive amount of data. Objectives of the project ** The objective of this PhD research is the development of a bioinformatics pipeline able to analyze microbial RNA-seq data based on the next-generation DNA-sequencing technologies.

bedtools: a powerful toolset for genome arithmetic — bedtools 2.30.0 documentation Collectively, the bedtools utilities are a swiss-army knife of tools for a wide-range of genomics analysis tasks. The most widely-used tools enable genome arithmetic: that is, set theory on the genome. For example, bedtools allows one to intersect, merge, count, complement, and shuffle genomic intervals from multiple files in widely-used genomic file formats such as BAM, BED, GFF/GTF, VCF. While each individual tool is designed to do a relatively simple task (e.g., intersect two interval files), quite sophisticated analyses can be conducted by combining multiple bedtools operations on the UNIX command line. bedtools is developed in the Quinlan laboratory at the University of Utah and benefits from fantastic contributions made by scientists worldwide. We have developed a fairly comprehensive tutorial that demonstrates both the basics, as well as some more advanced examples of how bedtools can help you in your research. Commands used:

Pacific Biosciences’ Software Upgrade Enhances De Novo Genome Assembly, Variant Calling and cDNA Transcript Analysis MENLO PARK, Calif., Jan. 29, 2013 (GLOBE NEWSWIRE) — Pacific Biosciences of California, Inc. (Nasdaq:PACB) provider of the PacBio® RS High Resolution Genetic Analyzer, today announced it is releasing a new software upgrade that provides higher quality genome assemblies with near perfect base level accuracy in addition to other key features. The software upgrade extends the range of projects that uniquely benefit from the company’s Single Molecule, Real-Time (SMRT®) DNA Sequencing method, and will be available to customers for download on January 31st. SMRT Analysis 1.4 includes a new hierarchical de novo genome assembly process (HGAP), which allows researchers to assemble entire microbial and fungal genomes solely using PacBio long reads. As a result, users can generate better assemblies with a single library preparation and fewer SMRT® Cells than previous approaches that also required short-read sequencing technologies or circular consensus sequencing. About Pacific Biosciences

18.04.6 LTS (Bionic Beaver) Ubuntu is distributed on three types of images described below. Desktop image The desktop image allows you to try Ubuntu without changing your computer at all, and at your option to install it permanently later. This type of image is what most people will want to use. 64-bit PC (AMD64) desktop image Choose this if you have a computer based on the AMD64 or EM64T architecture (e.g., Athlon64, Opteron, EM64T Xeon, Core 2). Server install image The server install image allows you to install Ubuntu permanently on a computer for use as a server. 64-bit PC (AMD64) server install image A full list of available files, including BitTorrent files, can be found below. If you need help burning these images to disk, see the Image Burning Guide.

Internship Available – Product Development – RNA-Seq Intern Product Development Job Location: Austin, TX The successful candidate will support product development activities for gene expression analysis through next generation sequencing (NGS). Throughout the course of the internship, the candidate will be trained in nucleic acid sample preparation and extraction techniques from different biological matrices, PCR, nucleic acid characterization, and library preparation for NGS, and . RequirementsIntern candidates must be enrolled at an accredited college or university pursuing a Bachelor’s, Master’s, or PhD degree. Typical LocationsAustin, TX The CompanyAt Life Technologies, we believe in the power of science to transform life and the power of people to make it happen. Job ID: 12987BR

: Lab is Simple Step 1: Download Ubuntu 18.04 from Link: Step 2: Install Ubuntu. Select install Open SSHSet the hard disk. You should give all hardisk you have for ubuntu_lv Step 3: Login as root: Login by account you use to create ubuntu 18.04go to root by command sudo -iSet password for root by command: passwdAllow root access over SSH run command: sed -i -e "s/. Step 2: Add repository to end of file: /etc/apt/sources.list deb [trusted=yes] Step 3: Make sure your server can connect to internetRun bellow commandsecho "nameserver 8.8.8.8" > /etc/resolv.confapt-get updateapt-get purge netplan.ioapt-get install pnetlab -y Note: May be get error in installing process. run apt-get update then install pnetlab again apt-get install pnetlab -y Reboot Step 4: Upgrade to the latest version. Follow this guide to upgrade to the latest version:

Featured Job – Senior Scientist – Pfizer Org Marketing Statement All over the world, Pfizer colleagues are working together to positively impact health for everyone, everywhere. Each position at Pfizer touches and contributes to the success of our business and our world. That’s why, as one of the global leaders in the biopharmaceutical industry, Pfizer is committed to seeking out inspired new talent who share our core values and mission of making the world a healthier place. Role Description We seek an highly motivated immunologist to work with members of the Immunoregulation Group within the Immunology and Autoimmunity Research Unit. The primary focus of the position will be to participate in the identification, evaluation and development of the next generation of novel therapies for autoimmune disease. Apply Now

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