Fatguy's Agar Technique
Agar inoculation has been used for cultivation mushrooms as well as bacteria and molds for several years. The key to using an agar solution in cultivating mushrooms is sterility. Keeping the work surface and your hands clean and free of contaminants is the most important thing in using agar. I recommend that a HEPA system be used but since most people that use this forum cannot afford one, a glove box can be successfully used or a homemade HEPA design (what I use) will work. Choosing an agar solution to work with should be pretty easy. I purchase agar from Fungi Perfecti but there are several different sources of suppliers out there. Agar production and pouring I use a Malt Extract Agar (MEA) solution that is shipped to me in a powder form. -Mix 50 grams of MEA (7 Tablespoons) for each liter of water. You can use a Mason Jar as a container to mix and autoclave the solution. When the 45 minutes is up, slowly and carefully lower the heat and the pressure of the pressure canner.
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