background preloader


Primers are short pieces of DNA that are made in a laboratory. Since they're custom built, primers can have any sequence of nucleotides you'd like. In a PCR experiment, two primers are designed to match to the segment of DNA you want to copy. Through complementary base pairing, one primer attaches to the top strand at one end of your segment of interest, and the other primer attaches to the bottom strand at the other end. In most cases, 2 primers that are 20 or so nucleotides long will target just one place in the entire genome. Primers are also necessary because DNA polymerase can't attach at just any old place and start copying away. DNA Polymerase is a naturally occurring complex of proteins whose function is to copy a cell's DNA before it divides in two. The DNA polymerase in our bodies breaks down at temperatures well below 95 °C (203 °F), the temperature necessary to separate two complementary strands of DNA in a test tube.


DNA Forensics: The latest national and international news about DNA databases and forensic science Contact Professor Manfred Kayser from the Erasmus University Medical Center in Rotterdam, the Netherlands, and his colleagues have identified five candidate genes associated with different facial shapes - known as PRDM16, PAX3, TP63, C5orf50, and COL17A1. During a press conference in Drachten, in Friesland, a Northern province of The Netherlands, the public prosecution's office announced that approximately 8,000 men have been asked to provide DNA samples to help solve the 1999 murder of Marianne Va ... On August 24th, in Fort Worth, Texas, David Lee Wiggins, walked out of the courthouse accompanied by his attorney Nina Morrison, from the Innocence Project, his brother Duane Wiggins and sister Candy Berg. Wiggins was convicted and sentenced to life ... A new forensic technique called Hirisplex will soon allow investigators to predict certain physical characteristics of suspects, like their hair and eye color, after analyzing traces of DNA that were left at the crime scene.

Bloodstain Pattern Analysis in Action: Chamberlain Case" In August 1980, the Chamberlain family was camping near a rock formation called Ayers Rock in Central Australia. Lindy Chamberlain put two of her children, 4-year-old Reagan and 10-week-old Azaria, to bed in their tent. When she returned, she cried "The dingo took my baby!" According to Lindy, when she got to the tent she saw a dingo dragging something out of it. She wasn't close enough to see what it was, but when she checked on the children she saw that Azaria was missing. As the cry went out, she and her husband Michael, along with other campers, began searching for Azaria. Gene Delivery: Tools of the Trade Genes can be delivered into a group of cells in a patient's body in two ways. The first, called in vivo (in VEE-voh), is to inject the vector directly into the patient, aiming to target the affected cells. The second, called ex vivo (ex VEE-voh), is to deliver the gene to cells that have been removed from the body and are growing in culture.

Five fingers of evolution - Paul Andersen In his talk, Paul Andersen explains the five causes of microevolution. Research one example for each cause in the human population. Use the following population simulator to simulate microevolution: Run the simulation using the default settings.

Who Ate the Cheese Objectives: In this simulation you will examine crime scene evidence to determine who is responsible for eating the Queen's special imported Lindbergher Cheese (yes, the stinky cheese). You will model the process of electrophoresis and DNA fingerprinting. Name:_______________________________________________Date:__________ DNA Evidence Evaluation 1. Genetic map of Britain goes on display A genetic map of the British people has been produced by Oxford University researchers. It forms the centrepiece of their display at the Royal Society's free Summer Science Exhibition, which opens today. The remarkable thing about the map is how much people sharing similar gene variations cluster geographically. The groupings often appear to match the separate historical pasts of different areas of Britain, following ancient enmities or reflecting differences that we hold onto today about where in the country we come from. On the genetic map of Britain, Cornish people clustered separately from those from Devon, while the Scottish and Irish tended to share the same DNA markers.

Azaria Chamberlain case: Dingo DID take baby rules coroner Mother served six years in jail for murdering tot who disappeared in the night during a camping trip to Ayers Rock in 1980She and her then husband had always maintained that a wild dog had snatched their childBaby's body was never found despite intensive searches by police, Aboriginal trackers and park rangersHer clothing was later found beside a dingo's lair at the base of the rockIncredible scenes as coroner fights back tears as she delivers the verdict at fourth inquest into the case By Richard Shears Published: 03:11 GMT, 12 June 2012 | Updated: 03:22 GMT, 13 June 2012

PCR The polymerase chain reaction (PCR) is a method to rapidly amplify sequences of DNA. During a typical PCR, template DNA (containing the region of interest) is mixed with deoxynucleotides (dNTPs), a DNA polymerase and primers. Primers are short segments of complimentary DNA that base-pair with the template DNA upstream of the region of interest and serve as recruitment sites for the polymerase. PCR involves a series of temperature cycles that, although once conducted by moving tubes through various water baths, is now controlled automatically by the use of thermal cyclers, or thermocyclers.

BBC Science - How does DNA testing work? 1 February 2013Last updated at 16:29 DNA testing is a powerful tool for identification and has many practical applications. Common uses include: