Characterization of P-Chloro-M-Cresol Abstract p-Chloro-m-cresol (PCMC) is widely used in pharmaceutical industries as biocide and preservative. However, it faces the problems of solubility in water and photo degradation. Keywords: Biofield treatment; p-chloro-m-cresol; X-ray diffraction; Surface area analysis; Differential scanning calorimetry; Thermogravimetric analysis; Fourier transform infrared spectroscopy; Ultraviolet-visible spectroscopy; Gas chromatography-mass spectrometry Introduction p-Chloro-m-cresol (PCMC) which is also known as chlorocresol (Figure 1), is used as an external germicide and bactericide agent. Figure 1: Chemical structure of p-chloro-m-cresol. Although PCMC is widely used in pharmaceutical preparations but its effectiveness was reduced due to some problems related to solubility and stability . Materials and Methods Sample preparation P-chloro-m-cresol (PCMC) was procured from Sisco Research Laboratories, India. X-ray diffraction (XRD) study G=kλ/ (bCosθ) Surface area analysis Spectroscopic studies 1.
Thiourea, Sodium Carbonate IR Spectrum Analysis Abstract The stability of any pharmaceutical compound is most desired quality that determines its shelf life and effectiveness.The stability can be correlated to structural and bonding properties of compound and any variation arise in these properties can be easily determined by spectroscopic analysis. The present study was aimed to evaluate the impact of biofield treatment on these properties of four pharmaceutical compounds such as urea, thiourea, sodium carbonate,and magnesium sulphate, using spectroscopic analysis. Each compound was divided into two groups, referred as control and treatment. The control groups remained as untreated and treatment group of each compound received Mr. Trivedi’s biofield treatment. Keywords: Urea; Thiourea; Sodium carbonate; Magnesium sulphate; Biofield treatment; Fourier transform infrared spectroscopy;Ultraviolet-visible spectroscopy Introduction Sodium carbonate, commonly known as washing soda, is sodium salt of carbonic acid. Materials and Methods 1.
Physical, Thermal & Spectral Properties of Butylated Hydroxytoluene Abstract The antioxidants play an important role in the preservation of foods and the management of oxidative stress related diseases by acting on reactive oxygen species and free radicals. However, their use in high temperature processed food and pharmaceuticals are limited due to its low thermal stability. The objective of the study was to use the biofield energy treatment on butylated hydroxytoluene (BHT) i.e. antioxidant and analyse its impact on the physical, thermal, and spectral properties of BHT. Keywords: Biofield energy treatment; Butylated hydroxytoluene; Reactive oxygen species; Complementary and alternative medicine; Thermogravimetric analysis Introduction In recent years, the studies on reactive oxygen species (ROS), free radicals and antioxidants are generating medical revolution by promising a good health and disease management . Figure 1: Chemical structure of butylated hydroxytoluene. In food preservation process, the thermal stability of antioxidant is very crucial.
Analysis of Phenol Derivatives Isotopic Abundance in BHT & 4-MP Abstract Butylatedhydroxytoluene (BHT) and 4-methoxyphenol (4-MP) are phenol derivatives that are generally known for their antioxidant properties and depigmenting activities. The aim of this study was to evaluate the impact of biofield energy treatment on the isotopic abundance in BHT and 4-MP using gas chromatography-mass spectrometry (GC-MS). BHT and 4-MP samples were divided into two parts: control and treated. The control group remained untreated while the treated group was subjected to Mr. Keywords: Biofield energy treatment; Butylatedhydroxytoluene; Gas chromatography-mass spectrometry; 4-methoxyphenol; Isotopic abundance Abbreviations GC-MS: Gas Chromatography-Mass spectrometry; PM: Primary Molecule; PM+1: Isotopic molecule either for 13C/12C or 2H/1H; PM+2:Isotopic molecule for 18O/16O; BHT: Butylatedhydroxytoluene; 4-MP:4-methoxyphenol Introduction For example, 13C, atom percent 13C=[13C/(12C+13C)] × 100 Experimental Both BHT and 4-MP were procured from SD Fine Chem. GC-MS method 1.
Thermal & Physical Properties of Silver Oxide Powder Abstract Silver oxide has gained significant attention due to its antimicrobial activities. The purpose of this study was to evaluate the impact of biofield energy treatment on the physical and thermal properties of silver oxide (Ag2O). The silver oxide powder was divided into two parts, one part was kept as control and another part was received Mr. Trivedi’s biofield energy treatment. The control and treated samples were analyzed using X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and Fourier transform infrared (FT-IR) spectroscopy. Keywords: Silver Oxide, Biofield Energy Treatment, X-Ray Diffraction, Differential Scanning Calorimetry, Thermogravimetric Analysis, Fourier Transform Infrared Spectroscopy 1. Silver (Ag) is a naturally occurring ductile and malleable element. 2. Silver oxide powder was procured from Sigma Aldrich, USA. 2.1. The crystallite size (D) was calculated by using Scherrer equation as following: D = kλ/(bCosθ) 3.
Alteration in Proteus Vulgaris Antimicrobials Susceptibility Pattern Abstract Proteus vulgaris (P. vulgaris) is widespread in nature, mainly found in flora of human gastrointestinal tract. The current study was attempted to investigate the effects of Mr. Keywords: Proteus vulgaris, Antimicrobial Susceptibility, Biofield Treatment, Biochemical Reaction, Biotype, 16S rDNA Analysis 1. Proteus vulgaris (P. vulgaris) is a genus of Gram-negative bacteria widespread in the environment and also found in normal gut flora of the human. Due to the clinical significance of this organism and literature reports on biofield treatment, the present work was undertaken to evaluate the impact of biofield treatment on P. vulgaris in relation to antimicrobials susceptibility and biotyping based on various biochemical characters followed by 16S rDNA sequencing analysis. 2. P. vulgaris, American Type Culture Collection (ATCC 33420) strain was procured from Micro BioLogics, Inc., USA and stored with proper storage conditions until further use. 2.1. Group IIB – Study I 2.2. 2.3.
Physical & Thermal Characteristics of Selenium Abstract Selenium (Se) is an essential trace element, and its deficiency in the humans leads to increase the risk of various diseases, such as cancer and heart diseases. The objective of this study was to investigate the influence of biofield energy treatment on the physical and thermal properties of the selenium powder. The selenium powder was divided into two parts denoted as control and treated. Keywords: Biofield Energy Treatment, Selenium Powder, X-ray Diffraction, Thermogravimetric Analysis – Differential Thermal Analysis, Differential Scanning Calorimetry,Fourier Transform Infrared 1. The importance of selenium (Se) in human is well established, and its deficiency has caused serious diseases such as cancer and heart disease . It is well established that all atoms are in motion, which contain significant amount of energy. 2. The selenium powder was purchased from Alpha Aesar, Hyderabad, India. 2.1. Further, the crystallite size (G) was calculated by using Scherrer formula: 2.2.
Study of Vibrio Parahaemolyticus Biochemical Reactions Abstract The recent emergence of the Vibrio parahaemolyticus (V. parahaemolyticus) is a pandemic. For the safety concern of seafood, consumer monitoring of this organism in seafood is very much essential. The current study was undertaken to evaluate the impact of Mr. Trivedi’s biofield energy treatment on [ATCC-17802] strain of V. parahaemolyticus for its biochemical characteristics, biotype and 16S rDNA analysis. Keywords: Vibrio parahaemolyticus, Biofield Energy Treatment, Biochemical Reaction, Biotype, 16S rDNA Analysis 1. Vibrio parahaemolyticus (V. parahaemolyticus) is a Gramnegative, human-pathogenic halophilic bacterium. As V. parahaemolyticus is an important foodborne pathogen. Based on the clinical importance of this organism in the field of seafood industry, the work was undertaken to assess the effect of biofield energy treatment on V. parahaemolyticus in relation to biochemical characteristics and biotyping followed by 16S rDNA sequencing. 2. 2.1. Group IIB – Study I 2.2. 2.3.
Biofield Impact on Tumor Cells - Two Time Lapse Video Microscopy Abstract Study background: Glioblastoma (GBM) is the most common subtype of primary brain tumor in adults. The aim was to evaluate the impact of biofield treatment potential on human GBM and non-GBM brain cells using two time-lapse video microscopy technique. Methods: The human brain tumor, GBM cultured cells were divided into two groups viz. Results: GBM control cells showed a basal level of cell death 10 hours prior and 10 hours after the biofield treatment, and the rate remained unchanged over the 20 hours period, while in treatment group of GBM, cell death rate was exponentially increased (41%) after biofield treatment as compared to control. Figure 5: Percent change of cell death after 20 hours treatment period with respect to control. Conclusion: Altogether, data suggests that biofield treatment has significantly increased the cell death rate of treated GBM cells and simultaneously boost the viability of normal brain cells. Introduction Materials and Methods Results and Discussion 1.
Impact of Energy Treatment on Lithium Powder Abstract Lithium has gained extensive attention in medical science due to mood stabilizing activity. The objective of the present study was to evaluate the impact of biofield treatment on physical, atomic, and thermal properties of lithium powder. The lithium powder was divided into two parts i.e., control and treatment. Control part was remained as untreated and treatment part received Mr. Trivedi’s biofield treatment. Keywords: Biofield treatment; Lithium; X-ray diffraction; Differential scanning calorimetry; Thermogravimetric analysis-differential thermal analysis; Scanning electron microscopy; Fourier transform infrared spectroscopy Introduction Lithium is highly reactive, light metal, which is commonly found in various foods such as grains, vegetables, mustard, kelp, and fish blue corn etc. which states that the moving charge produces magnetic fields in surrounding space [9,10]. used to measure the biofield of human body . Materials and Methods Biofield energy treatment Conclusion
Antimicrobial & Biotyping Analysis of Escherichia Coli Abstract Escherichia coli (E. coli) infections are the major health concern, as it causes infections in human mainly in urinary tract, ear, and wound infections. The present study evaluates the impact of biofield energy treatment on E. coli regarding antimicrobial sensitivity assay, biochemical study and biotype number. Four multidrug resistant (MDR) clinical lab isolates (LSs) of E. coli (LS 12, LS 13, LS 42, and LS 51) were taken in two groups i.e. control and treated. After treatment, above mentioned parameter were evaluated on day 10 in control and treated samples using MicroScan Walk-Away® system. Keywords: Escherichia coli, Biofield Energy Treatment, Multidrug-Resistant, Antibiogram, Biochemical, Biotyping 1. Escherichia coli (E. coli) is a Gram-negative, rod shape, and facultative anaerobic pathogen linked with communityassociated as well as nosocomial infections. 2. 2.1. The MDR clinical lab isolates of E. coli (i.e. 2.2. 2.3. 3. 3.1. 3.2. Table 4. 4. Abbreviations References
Impact of Biofield on Prostate Cancer Cell Lines Abstract Increasing cancer rates particularly in the developed world are associated with related lifestyle and environmental exposures. Combined immunotherapy and targeted therapies are the main treatment approaches in advanced and recurrent cancer. An alternate approach, energy medicine is increasingly used in life threatening problems to promote human wellness. Keywords: Biofield treatment; Cancer biomarker; ELISA; TNF-α; IL-6; Prostate cancer; Endometrium cancer Introduction Cancer has the potential to invade or spread to other parts of the body which involves abnormal cell growth. Role of immune cells estimation in microenvironment of tumor has been well established. In the last 70 years, cancer treatment strategies have rapidly increased. Materials and Methods Experimental design Endometrium and prostate cancer cell lines, as stock stored cultures were procured for the experiment from department of laboratory medicine, P.D. Biofield treatment modalities Results and Discussion Conclusion
Spectroscopic Characterization of Potato Micropropagation Medium Abstract Potato Micropropagation Medium (PMM) is the growth medium used for in vitro micropropagation of potato tubers. The present study was intended to assess the effect of biofield energy treatment on the physical, thermal and spectroscopic properties of PMM. The study was attained in two groups i.e. control and treated. The control group was remained as untreated, while the treated group was received Mr. Keywords: Biofield Energy Treatment, Potato Micropropagation Medium, X-ray Diffraction, Differential Scanning Calorimetry (DSC), UV-vis Spectroscopy, Fourier Transform Infrared Spectroscopy 1. Micropropagation is the technique of rapidly multiplying the stock plant material to generate a number of progeny plants, using advanced plant tissue culture methods [1, 2]. Biofield energy treatment is the part of energy therapy. Hence, after considering the impact of biofield treatment, the present study was aimed to evaluate the impact of biofield treatment on the PMM. 2. 2.1. Table 1. 2.2.
Energy Treatment and Shigella Sonnei Characteristics Abstract: Shigella sonnei (S. sonnei) is a non-motile, rod shape, clinically significant, Gram-negative bacterium. It is commonly associated with dysentery (shigellosis). Recently, resistance to third and fourth generation cephalosporins and fluoroquinolones has been reported in S. sonnei. In the present study, we assessed the effect of biofield treatment on phenotyping and genotyping characteristic of S. sonnei (ATCC 9290). Keywords: Antimicrobial susceptibility; Biofield treatment; 16S rDNA gene sequencing; Shigella sonnei Abbreviations: MIC: Minimum Inhibitory Concentration; ATCC: American Type Culture Collection; NBPC30: Negative Breakpoint Combo 30; NCBI: National Center for Biotechnology Information; WHO: World Health Organization; 16S rDNA: 16Svedberg Unit Ribosomal Deoxyribonucleic Acid; BLAST: Basic Local Alignment Search Tool; Outs: Operational Taxonomic Units Introduction Materials and Methods Biofield treatment Investigation of antimicrobial susceptibility of S. sonnei Results 1.