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Fish Peptone- Physical, Spectroscopic & Thermal Characterization

Fish Peptone- Physical, Spectroscopic & Thermal Characterization
Abstract The by-products of industrially processed fish are enzymatically converted into fish protein isolates and hydrolysates having a wide biological activity and nutritional properties. However, the heat processing may cause their thermal denaturation thereby causing the conformational changes in them. The present study utilized the strategy of biofield energy treatment and analysed its impact on various properties of the fish peptone as compared to the untreated (control) sample. The fish peptone sample was divided into two parts; one part was subjected to Mr. Trivedi’s biofield treatment, coded as the treated sample and another part was coded as the control. Keywords: Fish Peptone, Biofield Energy treatment, Protein Hydrolysate, Differential Scanning Calorimetry,Thermogravimetric Analysis 1. The fisheries industry is a major source of income for various countries worldwide. 2. The fish peptone was procured from HiMedia Laboratories, India. 2.1. 2.2. 2.3. 2.4. 2.5. 2.6. 2.7. 3. 4. Related:  Biofield Energy Treatment

Physicochemical & Spectroscopic Properties of Bio Peptone Abstract Bio peptone is a combination of enzymatic digest of animal tissues and casein; and generally used for the growth of several varieties of microbes. The aim of present study was to investigate the impact of biofield energy treatment on the physicochemical and spectroscopic properties of bio peptone. The present study was carried out in two groups i.e. control and treated. The control group was kept without treatment, while the treated group was subjected to Mr. Trivedi’s biofield energy treatment. Keywords: Bio Peptone, Biofield Energy Treatment, X-Ray Diffraction, Particle Size Analysis, Surface Area Analysis,Thermogravimetric Analysis, Fourier Transform Infrared Spectroscopy 1. A culture or growth medium is a liquid or gel, which is intended to support the growth of microorganism or cell [1]. Sterilization process plays a significant role on the quality of culture media. 2. 2.1. Bio peptone was procured from HiMedia Laboratories, India. 2.2. 2.3. 2.4. 2.5. 2.6. 2.7. 2.8. 2.9. 3.

Spectroscopic Characterization of Yeast Extract Powder Abstract Yeast extract powder (YE powder) is particularly used in culture media for the cultivation of microorganisms found in milk or other dairy products. The present study was intended to explore the influence of biofield energy treatment on the physicochemical and spectral properties of YE powder. Keywords: Yeast Extract Powder, the Trivedi Effect, Particle Size Analysis, Surface Area Analysis, Thermogravimetric Analysis, Fourier Transform Infrared Spectroscopy 1. Yeast extract powder (YE powder) is the common name used for several forms of processed yeast products [1]. Recently, the energy therapies have been reported for beneficial effects in the several fields throughout the world. The National Center for Complementary and Alternative Medicine (NCCAM) defines the biofield therapies as manipulation of various energy fields (measurable or putative) to affect the health. 2. The certified yeast extract (YE) powder was obtained from HiMedia Laboratories, India. 2.1. 2.2. 2.3. 2.4. 3.

Biofield Impact on Protose TGA Analysis Abstract Protose is the enzyme digest of mixed proteins that is recommended for culture media, bulk production of enzymes, antibiotics, toxins, veterinary preparations, etc. This study was proposed to evaluate the effect of biofield energy treatment on the physicochemical and spectroscopic properties of protose. The study was achieved in two groups i.e. control and treated. The control group was remained as untreated, while the treated group was received Mr. Trivedi’s biofield energy treatment. Keywords: Biofield Energy Treatment, Protose, X-ray Diffraction, Particle Size Analysis, Surface Area Analysis, Differential Scanning Calorimetry, Fourier Transform Infrared Spectroscopy 1. Growth medium or culture medium is a liquid or gel that is designed for the growth of microorganisms, cells or small plants such as moss [1]. Despite lots of applications of culture media, the thermal stability and chemical stability are the important attributes of any culture media. 2. 2.1. 2.2. 2.3. 2.4. 3.

Physical, Thermal & Spectral Properties of MS Media Abstract The Murashige and Skoog medium (MS media) is a chemically defined and widely used as a growth medium for plant tissue culture techniques. The present study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal, and spectral properties of MS media. The study was performed in two groups; one was kept as control while another was subjected to Mr. Keywords: Biofield Energy Treatment, Murashige and Skoog Medium, X-ray Diffraction, Fourier Transform Infrared Spectroscopy 1. The optimum growth and development of plant tissues are vary among the plants according to their nutritive requirements [1]. Recently, the energy therapies have been reported for several beneficial effects throughout the world. Therefore, after considering the significant impact of biofield energy treatment in different areas, this study was attempted to evaluate its impact on the plant cell culture media such as MS media. 2. 2.1. Table 1. 2.2. 2.3. 2.4. 2.5. 2.6. 3. 3.1. 3.2.

TGA Analysis of SFRE 199-1 Abstract SFRE 199-1 medium (SFRE-M) is important mammalian cell culture medium, used for the culture of primary cells of mammals such as baboon kidney cells. The present study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal and spectral properties of SFRE-M. The study was accomplished in two groups; one was set as control while another was subjected to Mr. Trivedi’s biofield energy treatment and coded as treated group. Subsequently, the control and treated samples were analyzed using various analytical techniques. Keywords: Biofield Energy Treatment, SFRE-Medium, Elemental Analysis, X-ray Diffraction, Fourier Transform Infrared Spectroscopy 1. Medium M-199 is a well-defined nutritional source for cell culture media, developed in 1950 by Morgan et al. [1, 2]. Sterilization process plays a significant role on the quality of culture media. Recently, the energy healing therapies have been reported for several beneficial effects throughout the word.

Spectroscopic Characterization of Potato Micropropagation Medium Abstract Potato Micropropagation Medium (PMM) is the growth medium used for in vitro micropropagation of potato tubers. The present study was intended to assess the effect of biofield energy treatment on the physical, thermal and spectroscopic properties of PMM. The study was attained in two groups i.e. control and treated. Keywords: Biofield Energy Treatment, Potato Micropropagation Medium, X-ray Diffraction, Differential Scanning Calorimetry (DSC), UV-vis Spectroscopy, Fourier Transform Infrared Spectroscopy 1. Micropropagation is the technique of rapidly multiplying the stock plant material to generate a number of progeny plants, using advanced plant tissue culture methods [1, 2]. Biofield energy treatment is the part of energy therapy. Hence, after considering the impact of biofield treatment, the present study was aimed to evaluate the impact of biofield treatment on the PMM. 2. 2.1. Potato micropropagation medium (PMM, Table 1) was obtained from HiMedia Laboratories, India. 2.2.

Impact of Biofield on Prostate Cancer Cell Lines Abstract Increasing cancer rates particularly in the developed world are associated with related lifestyle and environmental exposures. Combined immunotherapy and targeted therapies are the main treatment approaches in advanced and recurrent cancer. Keywords: Biofield treatment; Cancer biomarker; ELISA; TNF-α; IL-6; Prostate cancer; Endometrium cancer Introduction Cancer has the potential to invade or spread to other parts of the body which involves abnormal cell growth. Role of immune cells estimation in microenvironment of tumor has been well established. In the last 70 years, cancer treatment strategies have rapidly increased. Materials and Methods Experimental design Endometrium and prostate cancer cell lines, as stock stored cultures were procured for the experiment from department of laboratory medicine, P.D. Biofield treatment modalities Endometrium and prostate cancer cell lines were taken for biofield treatment. Measurement of diagnostic cancer biomarkers Results and Discussion 1.

Biofield Impact on Tumor Cells - Two Time Lapse Video Microscopy Abstract Study background: Glioblastoma (GBM) is the most common subtype of primary brain tumor in adults. The aim was to evaluate the impact of biofield treatment potential on human GBM and non-GBM brain cells using two time-lapse video microscopy technique. Methods: The human brain tumor, GBM cultured cells were divided into two groups viz. Results: GBM control cells showed a basal level of cell death 10 hours prior and 10 hours after the biofield treatment, and the rate remained unchanged over the 20 hours period, while in treatment group of GBM, cell death rate was exponentially increased (41%) after biofield treatment as compared to control. Figure 5: Percent change of cell death after 20 hours treatment period with respect to control. Conclusion: Altogether, data suggests that biofield treatment has significantly increased the cell death rate of treated GBM cells and simultaneously boost the viability of normal brain cells. Introduction Materials and Methods Results and Discussion 1.

Study of Vibrio Parahaemolyticus Biochemical Reactions Abstract The recent emergence of the Vibrio parahaemolyticus (V. parahaemolyticus) is a pandemic. For the safety concern of seafood, consumer monitoring of this organism in seafood is very much essential. The current study was undertaken to evaluate the impact of Mr. Keywords: Vibrio parahaemolyticus, Biofield Energy Treatment, Biochemical Reaction, Biotype, 16S rDNA Analysis 1. Vibrio parahaemolyticus (V. parahaemolyticus) is a Gramnegative, human-pathogenic halophilic bacterium. As V. parahaemolyticus is an important foodborne pathogen. Based on the clinical importance of this organism in the field of seafood industry, the work was undertaken to assess the effect of biofield energy treatment on V. parahaemolyticus in relation to biochemical characteristics and biotyping followed by 16S rDNA sequencing. 2. The strain of V. parahaemolyticus was bearing American Type Culture Collection (ATCC) number 17802, was obtained from MicroBioLogics, Inc., USA. 2.1. Group IIB – Study I 2.2. 2.3. 2.4.

Alteration in Proteus Vulgaris Antimicrobials Susceptibility Pattern Abstract Proteus vulgaris (P. vulgaris) is widespread in nature, mainly found in flora of human gastrointestinal tract. The current study was attempted to investigate the effects of Mr. Trivedi’s biofield treatment on lyophilized as well as revived state of P. vulgaris for antimicrobial susceptibility pattern, biochemical characteristics, and biotype. P. vulgaris cells were procured from Micro BioLogics Inc., USA, in sealed pack bearing the American Type Culture Collection (ATCC 33420) number and stored according to the recommended storage protocol until needed for experiments. Lyophilized vial of ATCC strain of P. vulgaris were divided in two parts, Gr. Keywords: Proteus vulgaris, Antimicrobial Susceptibility, Biofield Treatment, Biochemical Reaction, Biotype, 16S rDNA Analysis 1. Proteus vulgaris (P. vulgaris) is a genus of Gram-negative bacteria widespread in the environment and also found in normal gut flora of the human. 2. 2.1. Group IIB – Study I Group IIB – Study II 2.2. 2.3. 2.4.

Analysis of Phenol Derivatives Isotopic Abundance in BHT & 4-MP Abstract Butylatedhydroxytoluene (BHT) and 4-methoxyphenol (4-MP) are phenol derivatives that are generally known for their antioxidant properties and depigmenting activities. The aim of this study was to evaluate the impact of biofield energy treatment on the isotopic abundance in BHT and 4-MP using gas chromatography-mass spectrometry (GC-MS). BHT and 4-MP samples were divided into two parts: control and treated. Keywords: Biofield energy treatment; Butylatedhydroxytoluene; Gas chromatography-mass spectrometry; 4-methoxyphenol; Isotopic abundance Abbreviations GC-MS: Gas Chromatography-Mass spectrometry; PM: Primary Molecule; PM+1: Isotopic molecule either for 13C/12C or 2H/1H; PM+2:Isotopic molecule for 18O/16O; BHT: Butylatedhydroxytoluene; 4-MP:4-methoxyphenol Introduction Butylatedhydroxytoluene (BHT) is a crystalline stable solid, but it is light-sensitive and reactive to acid chlorides, acid anhydrides, and oxidizing agents. For example, 13C, atom percent 13C=[13C/(12C+13C)] × 100

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