Impact of Biofield on Prostate Cancer Cell Lines Abstract Increasing cancer rates particularly in the developed world are associated with related lifestyle and environmental exposures. Combined immunotherapy and targeted therapies are the main treatment approaches in advanced and recurrent cancer. An alternate approach, energy medicine is increasingly used in life threatening problems to promote human wellness. This study aimed to investigate the effect of biofield treatment on cancer biomarkers involved in human endometrium and prostate cancer cell lines. Each cancer cell lines were taken in two sealed tubes i.e. one tube was considered as control and another tube was subjected to Mr. Keywords: Biofield treatment; Cancer biomarker; ELISA; TNF-α; IL-6; Prostate cancer; Endometrium cancer Introduction Cancer has the potential to invade or spread to other parts of the body which involves abnormal cell growth. Role of immune cells estimation in microenvironment of tumor has been well established. Materials and Methods Experimental design 1.
TGA Analysis of SFRE 199-1 Abstract SFRE 199-1 medium (SFRE-M) is important mammalian cell culture medium, used for the culture of primary cells of mammals such as baboon kidney cells. The present study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal and spectral properties of SFRE-M. The study was accomplished in two groups; one was set as control while another was subjected to Mr. Trivedi’s biofield energy treatment and coded as treated group. Keywords: Biofield Energy Treatment, SFRE-Medium, Elemental Analysis, X-ray Diffraction, Fourier Transform Infrared Spectroscopy 1. Medium M-199 is a well-defined nutritional source for cell culture media, developed in 1950 by Morgan et al. [1, 2]. Sterilization process plays a significant role on the quality of culture media. Recently, the energy healing therapies have been reported for several beneficial effects throughout the word. 2. 2.1. The SFRE 199-1 media (SFRE-M) was procured from HiMedia Laboratories, India. Table 1. 3.
Biofield Impact on Protose TGA Analysis Abstract Protose is the enzyme digest of mixed proteins that is recommended for culture media, bulk production of enzymes, antibiotics, toxins, veterinary preparations, etc. This study was proposed to evaluate the effect of biofield energy treatment on the physicochemical and spectroscopic properties of protose. The study was achieved in two groups i.e. control and treated. The control group was remained as untreated, while the treated group was received Mr. Keywords: Biofield Energy Treatment, Protose, X-ray Diffraction, Particle Size Analysis, Surface Area Analysis, Differential Scanning Calorimetry, Fourier Transform Infrared Spectroscopy 1. Growth medium or culture medium is a liquid or gel that is designed for the growth of microorganisms, cells or small plants such as moss . Despite lots of applications of culture media, the thermal stability and chemical stability are the important attributes of any culture media. 2. 2.1. 2.2. 2.3. 2.4. 2.5. 2.6. 2.7. 3. 3.1. Fig. 1. 3.2. 3.3.
Biofield Impact on Tumor Cells - Two Time Lapse Video Microscopy Abstract Study background: Glioblastoma (GBM) is the most common subtype of primary brain tumor in adults. The aim was to evaluate the impact of biofield treatment potential on human GBM and non-GBM brain cells using two time-lapse video microscopy technique. Methods: The human brain tumor, GBM cultured cells were divided into two groups viz. GBM control and GBM treatment. Similarly, human normal brain cultured cells (non-GBM) were taken and divided into two groups viz. non- GBM control and non-GBM treatment. Results: GBM control cells showed a basal level of cell death 10 hours prior and 10 hours after the biofield treatment, and the rate remained unchanged over the 20 hours period, while in treatment group of GBM, cell death rate was exponentially increased (41%) after biofield treatment as compared to control. Figure 5: Percent change of cell death after 20 hours treatment period with respect to control. Introduction Materials and Methods Study design and biofield treatment strategy 1.
Study of Vibrio Parahaemolyticus Biochemical Reactions Abstract The recent emergence of the Vibrio parahaemolyticus (V. parahaemolyticus) is a pandemic. For the safety concern of seafood, consumer monitoring of this organism in seafood is very much essential. Keywords: Vibrio parahaemolyticus, Biofield Energy Treatment, Biochemical Reaction, Biotype, 16S rDNA Analysis 1. Vibrio parahaemolyticus (V. parahaemolyticus) is a Gramnegative, human-pathogenic halophilic bacterium. As V. parahaemolyticus is an important foodborne pathogen. Based on the clinical importance of this organism in the field of seafood industry, the work was undertaken to assess the effect of biofield energy treatment on V. parahaemolyticus in relation to biochemical characteristics and biotyping followed by 16S rDNA sequencing. 2. The strain of V. parahaemolyticus was bearing American Type Culture Collection (ATCC) number 17802, was obtained from MicroBioLogics, Inc., USA. 2.1. Group I: ATCC strain in the lyophilized state was considered as control. Group IIB – Study I 2.2.
Physicochemical & Spectroscopic Properties of Bio Peptone Abstract Bio peptone is a combination of enzymatic digest of animal tissues and casein; and generally used for the growth of several varieties of microbes. The aim of present study was to investigate the impact of biofield energy treatment on the physicochemical and spectroscopic properties of bio peptone. The present study was carried out in two groups i.e. control and treated. The control group was kept without treatment, while the treated group was subjected to Mr. Keywords: Bio Peptone, Biofield Energy Treatment, X-Ray Diffraction, Particle Size Analysis, Surface Area Analysis,Thermogravimetric Analysis, Fourier Transform Infrared Spectroscopy 1. A culture or growth medium is a liquid or gel, which is intended to support the growth of microorganism or cell . Sterilization process plays a significant role on the quality of culture media. In the recent past years, the energy therapies have been reported for beneficial effects in several field throughout the word . 2. 2.1. 2.2.
Fish Peptone- Physical, Spectroscopic & Thermal Characterization Abstract The by-products of industrially processed fish are enzymatically converted into fish protein isolates and hydrolysates having a wide biological activity and nutritional properties. However, the heat processing may cause their thermal denaturation thereby causing the conformational changes in them. The present study utilized the strategy of biofield energy treatment and analysed its impact on various properties of the fish peptone as compared to the untreated (control) sample. The fish peptone sample was divided into two parts; one part was subjected to Mr. Keywords: Fish Peptone, Biofield Energy treatment, Protein Hydrolysate, Differential Scanning Calorimetry,Thermogravimetric Analysis 1. The fisheries industry is a major source of income for various countries worldwide. The peptones are a mixture of polypeptides and amino acids that are used in several biotechnological applications. 2. The fish peptone was procured from HiMedia Laboratories, India. 2.1. 2.2. 2.3. 2.4. 2.5. 3.
Alteration in Proteus Vulgaris Antimicrobials Susceptibility Pattern Abstract Proteus vulgaris (P. vulgaris) is widespread in nature, mainly found in flora of human gastrointestinal tract. The current study was attempted to investigate the effects of Mr. Trivedi’s biofield treatment on lyophilized as well as revived state of P. vulgaris for antimicrobial susceptibility pattern, biochemical characteristics, and biotype. P. vulgaris cells were procured from Micro BioLogics Inc., USA, in sealed pack bearing the American Type Culture Collection (ATCC 33420) number and stored according to the recommended storage protocol until needed for experiments. Keywords: Proteus vulgaris, Antimicrobial Susceptibility, Biofield Treatment, Biochemical Reaction, Biotype, 16S rDNA Analysis 1. Proteus vulgaris (P. vulgaris) is a genus of Gram-negative bacteria widespread in the environment and also found in normal gut flora of the human. 2. 2.1. The impact of biofield treatment on tested bacterium P. vulgaris was evaluated in two groups- Group IIB – Study I Group IIB – Study II
Spectroscopic Characterization of Yeast Extract Powder Abstract Yeast extract powder (YE powder) is particularly used in culture media for the cultivation of microorganisms found in milk or other dairy products. The present study was intended to explore the influence of biofield energy treatment on the physicochemical and spectral properties of YE powder. The study was accomplished in two groups; first group was remained as control, while another was subjected to Mr. Trivedi’s biofield energy treatment and termed as the treated group. Afterward, both the samples were evaluated using several analytical techniques. Keywords: Yeast Extract Powder, the Trivedi Effect, Particle Size Analysis, Surface Area Analysis, Thermogravimetric Analysis, Fourier Transform Infrared Spectroscopy 1. Yeast extract powder (YE powder) is the common name used for several forms of processed yeast products . Recently, the energy therapies have been reported for beneficial effects in the several fields throughout the world. 2. 2.1. 2.2. 2.3. 2.4. 2.5. 2.6. 2.7. 3.
Analysis of Phenol Derivatives Isotopic Abundance in BHT & 4-MP Abstract Butylatedhydroxytoluene (BHT) and 4-methoxyphenol (4-MP) are phenol derivatives that are generally known for their antioxidant properties and depigmenting activities. The aim of this study was to evaluate the impact of biofield energy treatment on the isotopic abundance in BHT and 4-MP using gas chromatography-mass spectrometry (GC-MS). Keywords: Biofield energy treatment; Butylatedhydroxytoluene; Gas chromatography-mass spectrometry; 4-methoxyphenol; Isotopic abundance Abbreviations GC-MS: Gas Chromatography-Mass spectrometry; PM: Primary Molecule; PM+1: Isotopic molecule either for 13C/12C or 2H/1H; PM+2:Isotopic molecule for 18O/16O; BHT: Butylatedhydroxytoluene; 4-MP:4-methoxyphenol Introduction Butylatedhydroxytoluene (BHT) is a crystalline stable solid, but it is light-sensitive and reactive to acid chlorides, acid anhydrides, and oxidizing agents. For example, 13C, atom percent 13C=[13C/(12C+13C)] × 100 Experimental Both BHT and 4-MP were procured from SD Fine Chem. References
Physical, Thermal & Spectral Properties of Butylated Hydroxytoluene Abstract The antioxidants play an important role in the preservation of foods and the management of oxidative stress related diseases by acting on reactive oxygen species and free radicals. However, their use in high temperature processed food and pharmaceuticals are limited due to its low thermal stability. The objective of the study was to use the biofield energy treatment on butylated hydroxytoluene (BHT) i.e. antioxidant and analyse its impact on the physical, thermal, and spectral properties of BHT. For the study, the sample was divided into two groups and termed as control and treated. The treated group was subjected to biofield energy treatment. Keywords: Biofield energy treatment; Butylated hydroxytoluene; Reactive oxygen species; Complementary and alternative medicine; Thermogravimetric analysis Introduction Figure 1: Chemical structure of butylated hydroxytoluene. In food preservation process, the thermal stability of antioxidant is very crucial. Materials and Methods DSC analysis
Physical, Thermal & Spectral Properties of MS Media Abstract The Murashige and Skoog medium (MS media) is a chemically defined and widely used as a growth medium for plant tissue culture techniques. The present study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal, and spectral properties of MS media. Keywords: Biofield Energy Treatment, Murashige and Skoog Medium, X-ray Diffraction, Fourier Transform Infrared Spectroscopy 1. The optimum growth and development of plant tissues are vary among the plants according to their nutritive requirements . Recently, the energy therapies have been reported for several beneficial effects throughout the world. Therefore, after considering the significant impact of biofield energy treatment in different areas, this study was attempted to evaluate its impact on the plant cell culture media such as MS media. 2. 2.1. The MS media was procured from HiMedia Laboratories, India. Table 1. 2.2. 2.3. 2.4. 2.5. 2.6. 3. 3.1. Fig. 1. Fig. 2. 3.2. Fig. 3. 3.3. Fig. 4.
Physical, Spectral and Thermal Properties of Resorcinol Abstract Resorcinol is widely used in manufacturing of several drugs and pharmaceutical products that are mainly used for topical ailments. The main objective of this study is to use an alternative strategy i.e., biofield treatment to alter the physical, spectral and thermal properties of resorcinol. The resorcinol sample was divided in two groups, which served as control and treated group. The treated group was given biofield treatment and both groups i.e., control and treated were analysed using X-ray diffraction (XRD), Fourier transform-infrared (FT-IR) spectroscopy, UV-Visible (UVVis) spectroscopy, Differential scanning calorimetry (DSC) and Thermogravimetric analysis (TGA). Keywords: Resorcinol; Biofield energy treatment; X-Ray diffraction; Fourier transform infrared spectroscopy; Ultraviolet-Visible spectroscopy; Differential scanning calorimetry; Thermogravimetric analysis Abbreviations Introduction Materials and Methods Study design Resorcinol was procured from Loba Chemie Pvt. 1.