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Study of Vibrio Parahaemolyticus Biochemical Reactions

Study of Vibrio Parahaemolyticus Biochemical Reactions
Abstract The recent emergence of the Vibrio parahaemolyticus (V. parahaemolyticus) is a pandemic. For the safety concern of seafood, consumer monitoring of this organism in seafood is very much essential. The current study was undertaken to evaluate the impact of Mr. Trivedi’s biofield energy treatment on [ATCC-17802] strain of V. parahaemolyticus for its biochemical characteristics, biotype and 16S rDNA analysis. The lyophilized strain of V. parahaemolyticus was divided into two parts, Group (Gr.) Keywords: Vibrio parahaemolyticus, Biofield Energy Treatment, Biochemical Reaction, Biotype, 16S rDNA Analysis 1. Vibrio parahaemolyticus (V. parahaemolyticus) is a Gramnegative, human-pathogenic halophilic bacterium. As V. parahaemolyticus is an important foodborne pathogen. 2. The strain of V. parahaemolyticus was bearing American Type Culture Collection (ATCC) number 17802, was obtained from MicroBioLogics, Inc., USA. 2.1. Group IIB – Study I Group IIB – Study II 2.2. The lyophilized (Gr. 3. Related:  Biofield Energy Treatment

Biofield Impact on Tumor Cells - Two Time Lapse Video Microscopy Abstract Study background: Glioblastoma (GBM) is the most common subtype of primary brain tumor in adults. The aim was to evaluate the impact of biofield treatment potential on human GBM and non-GBM brain cells using two time-lapse video microscopy technique. Methods: The human brain tumor, GBM cultured cells were divided into two groups viz. GBM control and GBM treatment. Results: GBM control cells showed a basal level of cell death 10 hours prior and 10 hours after the biofield treatment, and the rate remained unchanged over the 20 hours period, while in treatment group of GBM, cell death rate was exponentially increased (41%) after biofield treatment as compared to control. Figure 5: Percent change of cell death after 20 hours treatment period with respect to control. Conclusion: Altogether, data suggests that biofield treatment has significantly increased the cell death rate of treated GBM cells and simultaneously boost the viability of normal brain cells. Introduction Conclusions 1.

Impact of Biofield on Prostate Cancer Cell Lines Abstract Increasing cancer rates particularly in the developed world are associated with related lifestyle and environmental exposures. Combined immunotherapy and targeted therapies are the main treatment approaches in advanced and recurrent cancer. An alternate approach, energy medicine is increasingly used in life threatening problems to promote human wellness. This study aimed to investigate the effect of biofield treatment on cancer biomarkers involved in human endometrium and prostate cancer cell lines. Keywords: Biofield treatment; Cancer biomarker; ELISA; TNF-α; IL-6; Prostate cancer; Endometrium cancer Introduction Cancer has the potential to invade or spread to other parts of the body which involves abnormal cell growth. Role of immune cells estimation in microenvironment of tumor has been well established. In the last 70 years, cancer treatment strategies have rapidly increased. Materials and Methods Experimental design Biofield treatment modalities Results and Discussion Conclusion

Alteration in Proteus Vulgaris Antimicrobials Susceptibility Pattern Abstract Proteus vulgaris (P. vulgaris) is widespread in nature, mainly found in flora of human gastrointestinal tract. The current study was attempted to investigate the effects of Mr. Trivedi’s biofield treatment on lyophilized as well as revived state of P. vulgaris for antimicrobial susceptibility pattern, biochemical characteristics, and biotype. Keywords: Proteus vulgaris, Antimicrobial Susceptibility, Biofield Treatment, Biochemical Reaction, Biotype, 16S rDNA Analysis 1. Proteus vulgaris (P. vulgaris) is a genus of Gram-negative bacteria widespread in the environment and also found in normal gut flora of the human. Due to the clinical significance of this organism and literature reports on biofield treatment, the present work was undertaken to evaluate the impact of biofield treatment on P. vulgaris in relation to antimicrobials susceptibility and biotyping based on various biochemical characters followed by 16S rDNA sequencing analysis. 2. 2.1. Group IIB – Study I 2.2. 2.3. 2.4.

Spectroscopic Characterization of Potato Micropropagation Medium Abstract Potato Micropropagation Medium (PMM) is the growth medium used for in vitro micropropagation of potato tubers. The present study was intended to assess the effect of biofield energy treatment on the physical, thermal and spectroscopic properties of PMM. The study was attained in two groups i.e. control and treated. The control group was remained as untreated, while the treated group was received Mr. Trivedi’s biofield energy treatment. Keywords: Biofield Energy Treatment, Potato Micropropagation Medium, X-ray Diffraction, Differential Scanning Calorimetry (DSC), UV-vis Spectroscopy, Fourier Transform Infrared Spectroscopy 1. Micropropagation is the technique of rapidly multiplying the stock plant material to generate a number of progeny plants, using advanced plant tissue culture methods [1, 2]. Biofield energy treatment is the part of energy therapy. 2. 2.1. Potato micropropagation medium (PMM, Table 1) was obtained from HiMedia Laboratories, India. Table 1. 2.2. 2.3. 2.4. 3.

TGA Analysis of SFRE 199-1 Abstract SFRE 199-1 medium (SFRE-M) is important mammalian cell culture medium, used for the culture of primary cells of mammals such as baboon kidney cells. The present study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal and spectral properties of SFRE-M. The study was accomplished in two groups; one was set as control while another was subjected to Mr. Keywords: Biofield Energy Treatment, SFRE-Medium, Elemental Analysis, X-ray Diffraction, Fourier Transform Infrared Spectroscopy 1. Medium M-199 is a well-defined nutritional source for cell culture media, developed in 1950 by Morgan et al. [1, 2]. Sterilization process plays a significant role on the quality of culture media. Recently, the energy healing therapies have been reported for several beneficial effects throughout the word. The energy medicines have been categorized by National Center for Complementary and Alternative Medicine (NCCAM) under the CAM therapies [12]. 2. 2.1. Table 1.

Analysis of Phenol Derivatives Isotopic Abundance in BHT & 4-MP Abstract Butylatedhydroxytoluene (BHT) and 4-methoxyphenol (4-MP) are phenol derivatives that are generally known for their antioxidant properties and depigmenting activities. The aim of this study was to evaluate the impact of biofield energy treatment on the isotopic abundance in BHT and 4-MP using gas chromatography-mass spectrometry (GC-MS). BHT and 4-MP samples were divided into two parts: control and treated. The control group remained untreated while the treated group was subjected to Mr. Trivedi’s biofield treatment. Keywords: Biofield energy treatment; Butylatedhydroxytoluene; Gas chromatography-mass spectrometry; 4-methoxyphenol; Isotopic abundance Abbreviations GC-MS: Gas Chromatography-Mass spectrometry; PM: Primary Molecule; PM+1: Isotopic molecule either for 13C/12C or 2H/1H; PM+2:Isotopic molecule for 18O/16O; BHT: Butylatedhydroxytoluene; 4-MP:4-methoxyphenol Introduction For example, 13C, atom percent 13C=[13C/(12C+13C)] × 100 Experimental GC-MS method Solution preparation 1.

Biofield Impact on Protose TGA Analysis Abstract Protose is the enzyme digest of mixed proteins that is recommended for culture media, bulk production of enzymes, antibiotics, toxins, veterinary preparations, etc. This study was proposed to evaluate the effect of biofield energy treatment on the physicochemical and spectroscopic properties of protose. The study was achieved in two groups i.e. control and treated. Keywords: Biofield Energy Treatment, Protose, X-ray Diffraction, Particle Size Analysis, Surface Area Analysis, Differential Scanning Calorimetry, Fourier Transform Infrared Spectroscopy 1. Growth medium or culture medium is a liquid or gel that is designed for the growth of microorganisms, cells or small plants such as moss [1]. Despite lots of applications of culture media, the thermal stability and chemical stability are the important attributes of any culture media. The energy therapies include magnet therapy, bio-electromagnetic therapy, healing touch, etc. and comprise low-level of energy field exchanges [10].

Physical, Thermal & Spectral Properties of Butylated Hydroxytoluene Abstract The antioxidants play an important role in the preservation of foods and the management of oxidative stress related diseases by acting on reactive oxygen species and free radicals. However, their use in high temperature processed food and pharmaceuticals are limited due to its low thermal stability. The objective of the study was to use the biofield energy treatment on butylated hydroxytoluene (BHT) i.e. antioxidant and analyse its impact on the physical, thermal, and spectral properties of BHT. For the study, the sample was divided into two groups and termed as control and treated. Keywords: Biofield energy treatment; Butylated hydroxytoluene; Reactive oxygen species; Complementary and alternative medicine; Thermogravimetric analysis Introduction In recent years, the studies on reactive oxygen species (ROS), free radicals and antioxidants are generating medical revolution by promising a good health and disease management [1]. Materials and Methods Material procurement DSC analysis

Physicochemical & Spectroscopic Properties of Bio Peptone Abstract Bio peptone is a combination of enzymatic digest of animal tissues and casein; and generally used for the growth of several varieties of microbes. The aim of present study was to investigate the impact of biofield energy treatment on the physicochemical and spectroscopic properties of bio peptone. The present study was carried out in two groups i.e. control and treated. Keywords: Bio Peptone, Biofield Energy Treatment, X-Ray Diffraction, Particle Size Analysis, Surface Area Analysis,Thermogravimetric Analysis, Fourier Transform Infrared Spectroscopy 1. A culture or growth medium is a liquid or gel, which is intended to support the growth of microorganism or cell [1]. Sterilization process plays a significant role on the quality of culture media. In the recent past years, the energy therapies have been reported for beneficial effects in several field throughout the word [10]. 2. 2.1. Bio peptone was procured from HiMedia Laboratories, India. 2.2. 2.3. 2.4. 2.5. 2.6. 2.7. 2.8. 3.

Physical, Spectral and Thermal Properties of Resorcinol Abstract Resorcinol is widely used in manufacturing of several drugs and pharmaceutical products that are mainly used for topical ailments. The main objective of this study is to use an alternative strategy i.e., biofield treatment to alter the physical, spectral and thermal properties of resorcinol. The resorcinol sample was divided in two groups, which served as control and treated group. Keywords: Resorcinol; Biofield energy treatment; X-Ray diffraction; Fourier transform infrared spectroscopy; Ultraviolet-Visible spectroscopy; Differential scanning calorimetry; Thermogravimetric analysis Abbreviations XRD: X-Ray Diffraction; FT-IR: Fourier Transform Infrared; DSC: Differential Scanning Calorimetry; TGA: Thermogravimetric Analysis; DTG: Derivative Thermogravimetry; NCCAM: National Centre for Complementary and Alternative Medicine Introduction Resorcinol is a dihydric phenol having the hydroxyl group at 1 and 3 positions in the benzene ring [1]. Materials and Methods Study design Conclusion

Fish Peptone- Physical, Spectroscopic & Thermal Characterization Abstract The by-products of industrially processed fish are enzymatically converted into fish protein isolates and hydrolysates having a wide biological activity and nutritional properties. However, the heat processing may cause their thermal denaturation thereby causing the conformational changes in them. The present study utilized the strategy of biofield energy treatment and analysed its impact on various properties of the fish peptone as compared to the untreated (control) sample. Keywords: Fish Peptone, Biofield Energy treatment, Protein Hydrolysate, Differential Scanning Calorimetry,Thermogravimetric Analysis 1. The fisheries industry is a major source of income for various countries worldwide. The peptones are a mixture of polypeptides and amino acids that are used in several biotechnological applications. The biofield energy treatment was reported as a measure for increasing the thermal stability of some organic products [15]. 2. 2.1. 2.2. 2.3. 2.4. 2.5. 2.6. 2.7. 3. 3.1. 3.2.

Characterization of P-Chloro-M-Cresol Abstract p-Chloro-m-cresol (PCMC) is widely used in pharmaceutical industries as biocide and preservative. However, it faces the problems of solubility in water and photo degradation. The aim of present study was to evaluate the impact of biofield treatment on physical, thermal and spectral properties of PCMC. Keywords: Biofield treatment; p-chloro-m-cresol; X-ray diffraction; Surface area analysis; Differential scanning calorimetry; Thermogravimetric analysis; Fourier transform infrared spectroscopy; Ultraviolet-visible spectroscopy; Gas chromatography-mass spectrometry Introduction p-Chloro-m-cresol (PCMC) which is also known as chlorocresol (Figure 1), is used as an external germicide and bactericide agent. Figure 1: Chemical structure of p-chloro-m-cresol. Although PCMC is widely used in pharmaceutical preparations but its effectiveness was reduced due to some problems related to solubility and stability [9]. Materials and Methods Sample preparation X-ray diffraction (XRD) study 1.

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