Analysis of Phenol Derivatives Isotopic Abundance in BHT & 4-MP Abstract Butylatedhydroxytoluene (BHT) and 4-methoxyphenol (4-MP) are phenol derivatives that are generally known for their antioxidant properties and depigmenting activities. The aim of this study was to evaluate the impact of biofield energy treatment on the isotopic abundance in BHT and 4-MP using gas chromatography-mass spectrometry (GC-MS). Keywords: Biofield energy treatment; Butylatedhydroxytoluene; Gas chromatography-mass spectrometry; 4-methoxyphenol; Isotopic abundance Abbreviations GC-MS: Gas Chromatography-Mass spectrometry; PM: Primary Molecule; PM+1: Isotopic molecule either for 13C/12C or 2H/1H; PM+2:Isotopic molecule for 18O/16O; BHT: Butylatedhydroxytoluene; 4-MP:4-methoxyphenol Introduction Butylatedhydroxytoluene (BHT) is a crystalline stable solid, but it is light-sensitive and reactive to acid chlorides, acid anhydrides, and oxidizing agents. For example, 13C, atom percent 13C=[13C/(12C+13C)] × 100 Experimental Both BHT and 4-MP were procured from SD Fine Chem. References
Study of Vibrio Parahaemolyticus Biochemical Reactions Abstract The recent emergence of the Vibrio parahaemolyticus (V. parahaemolyticus) is a pandemic. For the safety concern of seafood, consumer monitoring of this organism in seafood is very much essential. Keywords: Vibrio parahaemolyticus, Biofield Energy Treatment, Biochemical Reaction, Biotype, 16S rDNA Analysis 1. Vibrio parahaemolyticus (V. parahaemolyticus) is a Gramnegative, human-pathogenic halophilic bacterium. As V. parahaemolyticus is an important foodborne pathogen. Based on the clinical importance of this organism in the field of seafood industry, the work was undertaken to assess the effect of biofield energy treatment on V. parahaemolyticus in relation to biochemical characteristics and biotyping followed by 16S rDNA sequencing. 2. The strain of V. parahaemolyticus was bearing American Type Culture Collection (ATCC) number 17802, was obtained from MicroBioLogics, Inc., USA. 2.1. Group I: ATCC strain in the lyophilized state was considered as control. Group IIB – Study I 2.2.
Physical, Thermal & Spectral Properties of Butylated Hydroxytoluene Abstract The antioxidants play an important role in the preservation of foods and the management of oxidative stress related diseases by acting on reactive oxygen species and free radicals. However, their use in high temperature processed food and pharmaceuticals are limited due to its low thermal stability. The objective of the study was to use the biofield energy treatment on butylated hydroxytoluene (BHT) i.e. antioxidant and analyse its impact on the physical, thermal, and spectral properties of BHT. For the study, the sample was divided into two groups and termed as control and treated. The treated group was subjected to biofield energy treatment. Keywords: Biofield energy treatment; Butylated hydroxytoluene; Reactive oxygen species; Complementary and alternative medicine; Thermogravimetric analysis Introduction Figure 1: Chemical structure of butylated hydroxytoluene. In food preservation process, the thermal stability of antioxidant is very crucial. Materials and Methods DSC analysis
Biofield Impact on Tumor Cells - Two Time Lapse Video Microscopy Abstract Study background: Glioblastoma (GBM) is the most common subtype of primary brain tumor in adults. The aim was to evaluate the impact of biofield treatment potential on human GBM and non-GBM brain cells using two time-lapse video microscopy technique. Methods: The human brain tumor, GBM cultured cells were divided into two groups viz. GBM control and GBM treatment. Similarly, human normal brain cultured cells (non-GBM) were taken and divided into two groups viz. non- GBM control and non-GBM treatment. Results: GBM control cells showed a basal level of cell death 10 hours prior and 10 hours after the biofield treatment, and the rate remained unchanged over the 20 hours period, while in treatment group of GBM, cell death rate was exponentially increased (41%) after biofield treatment as compared to control. Figure 5: Percent change of cell death after 20 hours treatment period with respect to control. Introduction Materials and Methods Study design and biofield treatment strategy 1.
Physical, Spectral and Thermal Properties of Resorcinol Abstract Resorcinol is widely used in manufacturing of several drugs and pharmaceutical products that are mainly used for topical ailments. The main objective of this study is to use an alternative strategy i.e., biofield treatment to alter the physical, spectral and thermal properties of resorcinol. The resorcinol sample was divided in two groups, which served as control and treated group. The treated group was given biofield treatment and both groups i.e., control and treated were analysed using X-ray diffraction (XRD), Fourier transform-infrared (FT-IR) spectroscopy, UV-Visible (UVVis) spectroscopy, Differential scanning calorimetry (DSC) and Thermogravimetric analysis (TGA). Keywords: Resorcinol; Biofield energy treatment; X-Ray diffraction; Fourier transform infrared spectroscopy; Ultraviolet-Visible spectroscopy; Differential scanning calorimetry; Thermogravimetric analysis Abbreviations Introduction Materials and Methods Study design Resorcinol was procured from Loba Chemie Pvt. 1.
Impact of Biofield on Prostate Cancer Cell Lines Abstract Increasing cancer rates particularly in the developed world are associated with related lifestyle and environmental exposures. Combined immunotherapy and targeted therapies are the main treatment approaches in advanced and recurrent cancer. An alternate approach, energy medicine is increasingly used in life threatening problems to promote human wellness. This study aimed to investigate the effect of biofield treatment on cancer biomarkers involved in human endometrium and prostate cancer cell lines. Each cancer cell lines were taken in two sealed tubes i.e. one tube was considered as control and another tube was subjected to Mr. Keywords: Biofield treatment; Cancer biomarker; ELISA; TNF-α; IL-6; Prostate cancer; Endometrium cancer Introduction Cancer has the potential to invade or spread to other parts of the body which involves abnormal cell growth. Role of immune cells estimation in microenvironment of tumor has been well established. Materials and Methods Experimental design 1.
Characterization of P-Chloro-M-Cresol Abstract p-Chloro-m-cresol (PCMC) is widely used in pharmaceutical industries as biocide and preservative. However, it faces the problems of solubility in water and photo degradation. The aim of present study was to evaluate the impact of biofield treatment on physical, thermal and spectral properties of PCMC. For this study, PCMC sample was divided into two groups i.e., one served as treated and other as control. Keywords: Biofield treatment; p-chloro-m-cresol; X-ray diffraction; Surface area analysis; Differential scanning calorimetry; Thermogravimetric analysis; Fourier transform infrared spectroscopy; Ultraviolet-visible spectroscopy; Gas chromatography-mass spectrometry Introduction p-Chloro-m-cresol (PCMC) which is also known as chlorocresol (Figure 1), is used as an external germicide and bactericide agent. Figure 1: Chemical structure of p-chloro-m-cresol. Materials and Methods Sample preparation P-chloro-m-cresol (PCMC) was procured from Sisco Research Laboratories, India. 1.
Spectroscopic Characterization of Potato Micropropagation Medium Abstract Potato Micropropagation Medium (PMM) is the growth medium used for in vitro micropropagation of potato tubers. The present study was intended to assess the effect of biofield energy treatment on the physical, thermal and spectroscopic properties of PMM. Keywords: Biofield Energy Treatment, Potato Micropropagation Medium, X-ray Diffraction, Differential Scanning Calorimetry (DSC), UV-vis Spectroscopy, Fourier Transform Infrared Spectroscopy 1. Micropropagation is the technique of rapidly multiplying the stock plant material to generate a number of progeny plants, using advanced plant tissue culture methods [1, 2]. Biofield energy treatment is the part of energy therapy. Hence, after considering the impact of biofield treatment, the present study was aimed to evaluate the impact of biofield treatment on the PMM. 2. 2.1. Potato micropropagation medium (PMM, Table 1) was obtained from HiMedia Laboratories, India. Table 1. 2.2. G = [(Gt-Gc)/Gc] ×100 1 2.3. 2.4. 2.5. 2.6. 3. 3.1. 3.2.
Thiourea, Sodium Carbonate IR Spectrum Analysis Abstract The stability of any pharmaceutical compound is most desired quality that determines its shelf life and effectiveness.The stability can be correlated to structural and bonding properties of compound and any variation arise in these properties can be easily determined by spectroscopic analysis. The present study was aimed to evaluate the impact of biofield treatment on these properties of four pharmaceutical compounds such as urea, thiourea, sodium carbonate,and magnesium sulphate, using spectroscopic analysis. Each compound was divided into two groups, referred as control and treatment. Keywords: Urea; Thiourea; Sodium carbonate; Magnesium sulphate; Biofield treatment; Fourier transform infrared spectroscopy;Ultraviolet-visible spectroscopy Introduction Pharmaceutical industries are an important component of health care systems which are largely driven by scientific discovery and development of various chemical and biological agents for human and animal health. Study design 1.
TGA Analysis of SFRE 199-1 Abstract SFRE 199-1 medium (SFRE-M) is important mammalian cell culture medium, used for the culture of primary cells of mammals such as baboon kidney cells. The present study was attempted to evaluate the impact of biofield energy treatment on the physical, thermal and spectral properties of SFRE-M. The study was accomplished in two groups; one was set as control while another was subjected to Mr. Trivedi’s biofield energy treatment and coded as treated group. Keywords: Biofield Energy Treatment, SFRE-Medium, Elemental Analysis, X-ray Diffraction, Fourier Transform Infrared Spectroscopy 1. Medium M-199 is a well-defined nutritional source for cell culture media, developed in 1950 by Morgan et al. [1, 2]. Sterilization process plays a significant role on the quality of culture media. Recently, the energy healing therapies have been reported for several beneficial effects throughout the word. 2. 2.1. The SFRE 199-1 media (SFRE-M) was procured from HiMedia Laboratories, India. Table 1. 3.
Biofield Impact on Protose TGA Analysis Abstract Protose is the enzyme digest of mixed proteins that is recommended for culture media, bulk production of enzymes, antibiotics, toxins, veterinary preparations, etc. This study was proposed to evaluate the effect of biofield energy treatment on the physicochemical and spectroscopic properties of protose. The study was achieved in two groups i.e. control and treated. The control group was remained as untreated, while the treated group was received Mr. Keywords: Biofield Energy Treatment, Protose, X-ray Diffraction, Particle Size Analysis, Surface Area Analysis, Differential Scanning Calorimetry, Fourier Transform Infrared Spectroscopy 1. Growth medium or culture medium is a liquid or gel that is designed for the growth of microorganisms, cells or small plants such as moss . Despite lots of applications of culture media, the thermal stability and chemical stability are the important attributes of any culture media. 2. 2.1. 2.2. 2.3. 2.4. 2.5. 2.6. 2.7. 3. 3.1. Fig. 1. 3.2. 3.3.
Thermal & Physical Properties of Silver Oxide Powder Abstract Silver oxide has gained significant attention due to its antimicrobial activities. The purpose of this study was to evaluate the impact of biofield energy treatment on the physical and thermal properties of silver oxide (Ag2O). The silver oxide powder was divided into two parts, one part was kept as control and another part was received Mr. Keywords: Silver Oxide, Biofield Energy Treatment, X-Ray Diffraction, Differential Scanning Calorimetry, Thermogravimetric Analysis, Fourier Transform Infrared Spectroscopy 1. Silver (Ag) is a naturally occurring ductile and malleable element. 2. Silver oxide powder was procured from Sigma Aldrich, USA. 2.1. The XRD analysis of control and treated silver oxide was performed on Phillips, Holland PW 1710 X-ray diffractometer system. The crystallite size (D) was calculated by using Scherrer equation as following: D = kλ/(bCosθ) Here, λ =1.54056 Å, b is full width half maximum (FWHM) of peaks and k is the equipment constant (=0.94). 2.2. 2.3. 3. 4.
Physicochemical & Spectroscopic Properties of Bio Peptone Abstract Bio peptone is a combination of enzymatic digest of animal tissues and casein; and generally used for the growth of several varieties of microbes. The aim of present study was to investigate the impact of biofield energy treatment on the physicochemical and spectroscopic properties of bio peptone. The present study was carried out in two groups i.e. control and treated. The control group was kept without treatment, while the treated group was subjected to Mr. Keywords: Bio Peptone, Biofield Energy Treatment, X-Ray Diffraction, Particle Size Analysis, Surface Area Analysis,Thermogravimetric Analysis, Fourier Transform Infrared Spectroscopy 1. A culture or growth medium is a liquid or gel, which is intended to support the growth of microorganism or cell . Sterilization process plays a significant role on the quality of culture media. In the recent past years, the energy therapies have been reported for beneficial effects in several field throughout the word . 2. 2.1. 2.2.
Physical & Thermal Characteristics of Selenium Abstract Selenium (Se) is an essential trace element, and its deficiency in the humans leads to increase the risk of various diseases, such as cancer and heart diseases. The objective of this study was to investigate the influence of biofield energy treatment on the physical and thermal properties of the selenium powder. The selenium powder was divided into two parts denoted as control and treated. The Control part was remained as untreated and treated part received Mr. Trivedi’s biofield energy treatment. Keywords: Biofield Energy Treatment, Selenium Powder, X-ray Diffraction, Thermogravimetric Analysis – Differential Thermal Analysis, Differential Scanning Calorimetry,Fourier Transform Infrared 1. The importance of selenium (Se) in human is well established, and its deficiency has caused serious diseases such as cancer and heart disease . It is well established that all atoms are in motion, which contain significant amount of energy. 2. 2.1. G = kλ/(bCosθ), 2.2. 2.3. 3. 3.1. Fig 1.