Antimicrobial & Biotyping Analysis of Escherichia Coli Abstract Escherichia coli (E. coli) infections are the major health concern, as it causes infections in human mainly in urinary tract, ear, and wound infections. The present study evaluates the impact of biofield energy treatment on E. coli regarding antimicrobial sensitivity assay, biochemical study and biotype number. Four multidrug resistant (MDR) clinical lab isolates (LSs) of E. coli (LS 12, LS 13, LS 42, and LS 51) were taken in two groups i.e. control and treated. After treatment, above mentioned parameter were evaluated on day 10 in control and treated samples using MicroScan Walk-Away® system. Keywords: Escherichia coli, Biofield Energy Treatment, Multidrug-Resistant, Antibiogram, Biochemical, Biotyping 1. Escherichia coli (E. coli) is a Gram-negative, rod shape, and facultative anaerobic pathogen linked with communityassociated as well as nosocomial infections. 2. 2.1. The MDR clinical lab isolates of E. coli (i.e. 2.2. 2.3. 3. 3.1. 3.2. Table 4. 4. Abbreviations References
Physical & Thermal Characteristics of Selenium Abstract Selenium (Se) is an essential trace element, and its deficiency in the humans leads to increase the risk of various diseases, such as cancer and heart diseases. The objective of this study was to investigate the influence of biofield energy treatment on the physical and thermal properties of the selenium powder. The selenium powder was divided into two parts denoted as control and treated. Keywords: Biofield Energy Treatment, Selenium Powder, X-ray Diffraction, Thermogravimetric Analysis – Differential Thermal Analysis, Differential Scanning Calorimetry,Fourier Transform Infrared 1. The importance of selenium (Se) in human is well established, and its deficiency has caused serious diseases such as cancer and heart disease . It is well established that all atoms are in motion, which contain significant amount of energy. 2. The selenium powder was purchased from Alpha Aesar, Hyderabad, India. 2.1. Further, the crystallite size (G) was calculated by using Scherrer formula: 2.2.
Energy Treatment and Shigella Sonnei Characteristics Abstract: Shigella sonnei (S. sonnei) is a non-motile, rod shape, clinically significant, Gram-negative bacterium. It is commonly associated with dysentery (shigellosis). Recently, resistance to third and fourth generation cephalosporins and fluoroquinolones has been reported in S. sonnei. In the present study, we assessed the effect of biofield treatment on phenotyping and genotyping characteristic of S. sonnei (ATCC 9290). Keywords: Antimicrobial susceptibility; Biofield treatment; 16S rDNA gene sequencing; Shigella sonnei Abbreviations: MIC: Minimum Inhibitory Concentration; ATCC: American Type Culture Collection; NBPC30: Negative Breakpoint Combo 30; NCBI: National Center for Biotechnology Information; WHO: World Health Organization; 16S rDNA: 16Svedberg Unit Ribosomal Deoxyribonucleic Acid; BLAST: Basic Local Alignment Search Tool; Outs: Operational Taxonomic Units Introduction Materials and Methods Biofield treatment Investigation of antimicrobial susceptibility of S. sonnei Results 1.
Thermal & Physical Properties of Silver Oxide Powder Abstract Silver oxide has gained significant attention due to its antimicrobial activities. The purpose of this study was to evaluate the impact of biofield energy treatment on the physical and thermal properties of silver oxide (Ag2O). The silver oxide powder was divided into two parts, one part was kept as control and another part was received Mr. Trivedi’s biofield energy treatment. The control and treated samples were analyzed using X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and Fourier transform infrared (FT-IR) spectroscopy. Keywords: Silver Oxide, Biofield Energy Treatment, X-Ray Diffraction, Differential Scanning Calorimetry, Thermogravimetric Analysis, Fourier Transform Infrared Spectroscopy 1. Silver (Ag) is a naturally occurring ductile and malleable element. 2. Silver oxide powder was procured from Sigma Aldrich, USA. 2.1. The crystallite size (D) was calculated by using Scherrer equation as following: D = kλ/(bCosθ) 3.
Study of Multidrug Resistant Strain of K. Oxytoca Abstract Klebsiella are opportunistic pathogens that cause a wide spectrum of severe diseases. The aim of the present study was to investigate the impact of biofield treatment on multidrug resistant strain of K. oxytoca with respect to antibiogram pattern along with biochemical study and biotype number. Clinical lab isolate of K. oxytoca was divided into two groups i.e. control and treated. Keywords: Klebsiella oxytoca ; Multidrug resistant; Antibiogram; Biofield treatment; Biochemical reactions; Biotyping Introduction Klebsiella oxytoca (K. oxytoca) is a Gram-negative pathogen, cylindrical rod shaped, non-motile in nature, and belongs to Enterobacteriaceae family. Biofield is a cumulative outcome of electric and magnetic field energy, exerted by the human body. Materials and Methods Experimental design and biofield treatment MDR clinical strain of K. oxytoca was collected from stored stock cultures of clinical sample in Microbiology Lab, Hinduja Hospital, Mumbai. Biochemical reaction study
Klebsiella Pneumoniae Biochemical Characteristics Abstract: Pathogenic isolates of Klebsiella pneumoniae (K. pneumoniae), particularly the extended-spectrum β-lactamase (ESBL) producing strains, are mostly associated with the failure of antibiotic therapy in nosocomial infections. The present work was designed to evaluate the impact of Mr. Trivedi’s biofield energy treatment on phenotypic and genotypic characteristics of K. pneumoniae. The strain of K. pneumoniae bearing ATCC 15380 (American Type Culture Collection) was procured from the Bangalore Genei, in sealed pack and divided into control and treated groups. Keywords: Klebsiella pneumoniae; Biofield energy treatment; Antibiogram, Biochemical reactions, Polymorphism; Random Amplified Polymorphic DNA. Abbreviations: Introduction The increased medical practice for antibiotic usage creates selection pressure and results emergence of nosocomial pathogens. Materials and Methods Biofield treatment modalities K. pneumoniae strain was divided into two groups i.e. control and treated. Conclusion
Thiourea, Sodium Carbonate IR Spectrum Analysis Abstract The stability of any pharmaceutical compound is most desired quality that determines its shelf life and effectiveness.The stability can be correlated to structural and bonding properties of compound and any variation arise in these properties can be easily determined by spectroscopic analysis. The present study was aimed to evaluate the impact of biofield treatment on these properties of four pharmaceutical compounds such as urea, thiourea, sodium carbonate,and magnesium sulphate, using spectroscopic analysis. Each compound was divided into two groups, referred as control and treatment. The control groups remained as untreated and treatment group of each compound received Mr. Trivedi’s biofield treatment. Keywords: Urea; Thiourea; Sodium carbonate; Magnesium sulphate; Biofield treatment; Fourier transform infrared spectroscopy;Ultraviolet-visible spectroscopy Introduction Sodium carbonate, commonly known as washing soda, is sodium salt of carbonic acid. Materials and Methods 1.
Assessment of Shigella Boydii Characteristics Abstract Bacillary dysentery and acute gastroenteritis caused by infection of Shigella species are major public health burden in India and its neighboring countries. Emergence of antimicrobial resistance threatens to render current treatments ineffective. The current study was attempted to investigate the effect of biofield treatment on Shigella boydii (S. boydiii) with respect of antimicrobial susceptibility assay, biochemical characteristics and biotyping. Keywords: Shigella boydii, Antimicrobial Sensitivity, Biofield Treatment, Biochemical Reaction, Biotype, Bacillary Dysentery, Shigellosis, Acute Gastroenteritis 1. Shigella boydii (S. boydiii) is a non-motile, non-spore forming, non-lactose fermenting and Gram-negative rod shape bacterium that belongs to the family of Enterobacteriaceae. 2. S. boydiii, American Type Culture Collection (ATCC 9207) strains were procured from Micro Bio Logics, Inc., USA, in two sets A and B. 2.1. 2.2. The Gr. 2.3. 2.4. 2.5. 3. 3.1. Table 2. 3.2. 3.3.
Characterization of P-Chloro-M-Cresol Abstract p-Chloro-m-cresol (PCMC) is widely used in pharmaceutical industries as biocide and preservative. However, it faces the problems of solubility in water and photo degradation. Keywords: Biofield treatment; p-chloro-m-cresol; X-ray diffraction; Surface area analysis; Differential scanning calorimetry; Thermogravimetric analysis; Fourier transform infrared spectroscopy; Ultraviolet-visible spectroscopy; Gas chromatography-mass spectrometry Introduction p-Chloro-m-cresol (PCMC) which is also known as chlorocresol (Figure 1), is used as an external germicide and bactericide agent. Figure 1: Chemical structure of p-chloro-m-cresol. Although PCMC is widely used in pharmaceutical preparations but its effectiveness was reduced due to some problems related to solubility and stability . Materials and Methods Sample preparation P-chloro-m-cresol (PCMC) was procured from Sisco Research Laboratories, India. X-ray diffraction (XRD) study G=kλ/ (bCosθ) Surface area analysis Spectroscopic studies 1.
Providencia Rettgeri Biochemical Reactions Analysis Abstract Providencia rettgeri (P. rettgeri) is the key organism for gastrointestinal tract infections due to its high virulence properties. The current study was designed to investigate the effect of Mr. Trivedi’s biofield energy treatment on P. rettgeri in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. The lyophilized strain of P. rettgeri (ATCC 9250) was divided into two parts, Group (Gr.) Keywords: Providencia rettgeri, Antimicrobial Sensitivity, Minimum Inhibitory Concentration, Biofield Treatment,Biochemical Reaction, Biotype 1. Providencia rettgeri (P. rettgeri) is the key organism for gastrointestinal tract infections due to its high virulence properties. 2. The strain P. rettgeri, bearing the American Type Culture Collection (ATCC 9250) strain was procured from MicroBioLogics, Inc., USA. 2.1. The impact of biofield treatment on tested bacterium P. rettgeri was evaluated in two groups- 2.2. 2.3. 2.4.
Physical, Spectral and Thermal Properties of Resorcinol Abstract Resorcinol is widely used in manufacturing of several drugs and pharmaceutical products that are mainly used for topical ailments. The main objective of this study is to use an alternative strategy i.e., biofield treatment to alter the physical, spectral and thermal properties of resorcinol. Keywords: Resorcinol; Biofield energy treatment; X-Ray diffraction; Fourier transform infrared spectroscopy; Ultraviolet-Visible spectroscopy; Differential scanning calorimetry; Thermogravimetric analysis Abbreviations XRD: X-Ray Diffraction; FT-IR: Fourier Transform Infrared; DSC: Differential Scanning Calorimetry; TGA: Thermogravimetric Analysis; DTG: Derivative Thermogravimetry; NCCAM: National Centre for Complementary and Alternative Medicine Introduction Resorcinol is a dihydric phenol having the hydroxyl group at 1 and 3 positions in the benzene ring . Materials and Methods Study design Resorcinol was procured from Loba Chemie Pvt. X-ray diffraction (XRD) study G=kλ/(bCosθ) FT-IR analysis