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Antimicrobial & Biotyping Analysis of Escherichia Coli

Antimicrobial & Biotyping Analysis of Escherichia Coli
Abstract Escherichia coli (E. coli) infections are the major health concern, as it causes infections in human mainly in urinary tract, ear, and wound infections. The present study evaluates the impact of biofield energy treatment on E. coli regarding antimicrobial sensitivity assay, biochemical study and biotype number. Keywords: Escherichia coli, Biofield Energy Treatment, Multidrug-Resistant, Antibiogram, Biochemical, Biotyping 1. Escherichia coli (E. coli) is a Gram-negative, rod shape, and facultative anaerobic pathogen linked with communityassociated as well as nosocomial infections. Alternative medicine remains alternative due to their serious challenges against mainstream biomedical paradigm, as it requires a new framework. Due to the clinical significance of E. coli, present work was designed to study the impact of biofield energy treatment on MDR isolates of E. coli with respect to its antimicrobials susceptibility, biochemical reactions pattern, and biotype number. 2. 2.1. 2.2.

Impact of Energy Treatment on Lithium Powder Abstract Lithium has gained extensive attention in medical science due to mood stabilizing activity. The objective of the present study was to evaluate the impact of biofield treatment on physical, atomic, and thermal properties of lithium powder. The lithium powder was divided into two parts i.e., control and treatment. Keywords: Biofield treatment; Lithium; X-ray diffraction; Differential scanning calorimetry; Thermogravimetric analysis-differential thermal analysis; Scanning electron microscopy; Fourier transform infrared spectroscopy Introduction Lithium is highly reactive, light metal, which is commonly found in various foods such as grains, vegetables, mustard, kelp, and fish blue corn etc. Harold Saton Burr had performed the detailed studies on the correlation of electric current with physiological process and concluded that every single process in the human body had an electrical significance [8]. used to measure the biofield of human body [11]. Materials and Methods G=kλ/(bCosθ),

Energy Treatment and Shigella Sonnei Characteristics Abstract: Shigella sonnei (S. sonnei) is a non-motile, rod shape, clinically significant, Gram-negative bacterium. It is commonly associated with dysentery (shigellosis). Keywords: Antimicrobial susceptibility; Biofield treatment; 16S rDNA gene sequencing; Shigella sonnei Abbreviations: MIC: Minimum Inhibitory Concentration; ATCC: American Type Culture Collection; NBPC30: Negative Breakpoint Combo 30; NCBI: National Center for Biotechnology Information; WHO: World Health Organization; 16S rDNA: 16Svedberg Unit Ribosomal Deoxyribonucleic Acid; BLAST: Basic Local Alignment Search Tool; Outs: Operational Taxonomic Units Introduction Development of antimicrobial resistance in several microbes like bacteria, viruses, fungi, or in parasites has been reported globally in the recent few decades. Materials and Methods Two lyophilized vials of S. sonnei [American Type Culture Collection (ATCC) 9290] were purchased from MicroBioLogics, Inc., USA. Biofield treatment Biochemical studies Biotype number 1.

Study of Multidrug Resistant Strain of K. Oxytoca Abstract Klebsiella are opportunistic pathogens that cause a wide spectrum of severe diseases. The aim of the present study was to investigate the impact of biofield treatment on multidrug resistant strain of K. oxytoca with respect to antibiogram pattern along with biochemical study and biotype number. Clinical lab isolate of K. oxytoca was divided into two groups i.e. control and treated. Control group remain untreated and treated group was subjected to Mr. Trivedi’s biofield. Keywords: Klebsiella oxytoca ; Multidrug resistant; Antibiogram; Biofield treatment; Biochemical reactions; Biotyping Introduction Klebsiella oxytoca (K. oxytoca) is a Gram-negative pathogen, cylindrical rod shaped, non-motile in nature, and belongs to Enterobacteriaceae family. Biofield is a cumulative outcome of electric and magnetic field energy, exerted by the human body. Materials and Methods Experimental design and biofield treatment Evaluation of antimicrobial susceptibility assay Biochemical reaction study 1.

Physical & Thermal Characteristics of Selenium Abstract Selenium (Se) is an essential trace element, and its deficiency in the humans leads to increase the risk of various diseases, such as cancer and heart diseases. The objective of this study was to investigate the influence of biofield energy treatment on the physical and thermal properties of the selenium powder. The selenium powder was divided into two parts denoted as control and treated. The Control part was remained as untreated and treated part received Mr. Trivedi’s biofield energy treatment. Keywords: Biofield Energy Treatment, Selenium Powder, X-ray Diffraction, Thermogravimetric Analysis – Differential Thermal Analysis, Differential Scanning Calorimetry,Fourier Transform Infrared 1. The importance of selenium (Se) in human is well established, and its deficiency has caused serious diseases such as cancer and heart disease [1]. It is well established that all atoms are in motion, which contain significant amount of energy. 2. 2.1. G = kλ/(bCosθ), 2.2. 2.3. 3. 3.1. Fig 1.

Klebsiella Pneumoniae Biochemical Characteristics Abstract: Pathogenic isolates of Klebsiella pneumoniae (K. pneumoniae), particularly the extended-spectrum β-lactamase (ESBL) producing strains, are mostly associated with the failure of antibiotic therapy in nosocomial infections. The present work was designed to evaluate the impact of Mr. Keywords: Klebsiella pneumoniae; Biofield energy treatment; Antibiogram, Biochemical reactions, Polymorphism; Random Amplified Polymorphic DNA. Abbreviations: CAM: Complementary and Alternate Medicine; NHIS: National Health Interview Survey; NCHS: National Center for Health Statistics; ATCC: American Type Culture Collection; MIC: Minimum Inhibitory Concentration; MEGA: Molecular Evolutionary Genetics Analysis; NBPC 30: Negative Breakpoint Combo Panel 30; RAPD: Random Amplified Polymorphic DNA; PCR: Polymerase chain reaction; ESBL: Extended Spectrum β-Lactamase Introduction The increased medical practice for antibiotic usage creates selection pressure and results emergence of nosocomial pathogens. 1.

Thermal & Physical Properties of Silver Oxide Powder Abstract Silver oxide has gained significant attention due to its antimicrobial activities. The purpose of this study was to evaluate the impact of biofield energy treatment on the physical and thermal properties of silver oxide (Ag2O). The silver oxide powder was divided into two parts, one part was kept as control and another part was received Mr. Keywords: Silver Oxide, Biofield Energy Treatment, X-Ray Diffraction, Differential Scanning Calorimetry, Thermogravimetric Analysis, Fourier Transform Infrared Spectroscopy 1. Silver (Ag) is a naturally occurring ductile and malleable element. 2. Silver oxide powder was procured from Sigma Aldrich, USA. 2.1. The XRD analysis of control and treated silver oxide was performed on Phillips, Holland PW 1710 X-ray diffractometer system. The crystallite size (D) was calculated by using Scherrer equation as following: D = kλ/(bCosθ) Here, λ =1.54056 Å, b is full width half maximum (FWHM) of peaks and k is the equipment constant (=0.94). 2.2. 2.3. 3. 4.

Assessment of Shigella Boydii Characteristics Abstract Bacillary dysentery and acute gastroenteritis caused by infection of Shigella species are major public health burden in India and its neighboring countries. Emergence of antimicrobial resistance threatens to render current treatments ineffective. The current study was attempted to investigate the effect of biofield treatment on Shigella boydii (S. boydiii) with respect of antimicrobial susceptibility assay, biochemical characteristics and biotyping. The American Type Culture Collection (ATCC 9207) strain of S. boydiii was used in this experiment. The study was conducted in revived and lyophilized state of S. boydiii. Keywords: Shigella boydii, Antimicrobial Sensitivity, Biofield Treatment, Biochemical Reaction, Biotype, Bacillary Dysentery, Shigellosis, Acute Gastroenteritis 1. Shigella boydii (S. boydiii) is a non-motile, non-spore forming, non-lactose fermenting and Gram-negative rod shape bacterium that belongs to the family of Enterobacteriaceae. 2. 2.1. 2.2. The Gr. 2.3.

Providencia Rettgeri Biochemical Reactions Analysis Abstract Providencia rettgeri (P. rettgeri) is the key organism for gastrointestinal tract infections due to its high virulence properties. The current study was designed to investigate the effect of Mr. Keywords: Providencia rettgeri, Antimicrobial Sensitivity, Minimum Inhibitory Concentration, Biofield Treatment,Biochemical Reaction, Biotype 1. Providencia rettgeri (P. rettgeri) is the key organism for gastrointestinal tract infections due to its high virulence properties. Due to the clinical significance of this organism and literature reports on biofield treatment, the present work was undertaken to evaluate the impact of biofield treatment modality on P. rettgeri in relation to the antimicrobials susceptibility, biochemical reactions, and biotyping. 2. The strain P. rettgeri, bearing the American Type Culture Collection (ATCC 9250) strain was procured from MicroBioLogics, Inc., USA. 2.1. The impact of biofield treatment on tested bacterium P. rettgeri was evaluated in two groups- 3.

Thiourea, Sodium Carbonate IR Spectrum Analysis Abstract The stability of any pharmaceutical compound is most desired quality that determines its shelf life and effectiveness.The stability can be correlated to structural and bonding properties of compound and any variation arise in these properties can be easily determined by spectroscopic analysis. The present study was aimed to evaluate the impact of biofield treatment on these properties of four pharmaceutical compounds such as urea, thiourea, sodium carbonate,and magnesium sulphate, using spectroscopic analysis. Each compound was divided into two groups, referred as control and treatment. Keywords: Urea; Thiourea; Sodium carbonate; Magnesium sulphate; Biofield treatment; Fourier transform infrared spectroscopy;Ultraviolet-visible spectroscopy Introduction Pharmaceutical industries are an important component of health care systems which are largely driven by scientific discovery and development of various chemical and biological agents for human and animal health. Study design 1.

Antimycobacterial Susceptibility Testing of Antimicrobials Abstract The aim was to evaluate the impact of biofield treatment modality on mycobacterial strains in relation to antimycobacterials susceptibility. Mycobacterial sensitivity was analysed using 12 B BACTEC vials on the BACTEC 460 TB machine in 39 lab isolates (sputum samples) from stored stock cultures. Two American Type Culture Collection (ATCC) strains were also used to assess the minimum inhibitory concentration (MIC) of antimicrobials (Mycobacterium smegmatis 14468 and Mycobacterium tuberculosis 25177). Rifampicin, ethambutol and streptomycin in treated samples showed increased susceptibility as 3.33%, 3.33% and 400.6%, respectively, as compared to control in extensive drug resistance (XDR) strains. Pyrazinamide showed 300% susceptibility as compared to control in multidrug resistance (MDR) strains. Keywords: Antimycobacterial susceptibility; Biofield treatment;MDR-TB; XDR-TB; BACTEC 460/MGIT-TB; MIC Introduction Global tuberculosis control is facing major challenges now days. Mr.

Characterization of P-Chloro-M-Cresol Abstract p-Chloro-m-cresol (PCMC) is widely used in pharmaceutical industries as biocide and preservative. However, it faces the problems of solubility in water and photo degradation. The aim of present study was to evaluate the impact of biofield treatment on physical, thermal and spectral properties of PCMC. For this study, PCMC sample was divided into two groups i.e., one served as treated and other as control. Keywords: Biofield treatment; p-chloro-m-cresol; X-ray diffraction; Surface area analysis; Differential scanning calorimetry; Thermogravimetric analysis; Fourier transform infrared spectroscopy; Ultraviolet-visible spectroscopy; Gas chromatography-mass spectrometry Introduction p-Chloro-m-cresol (PCMC) which is also known as chlorocresol (Figure 1), is used as an external germicide and bactericide agent. Figure 1: Chemical structure of p-chloro-m-cresol. Materials and Methods Sample preparation P-chloro-m-cresol (PCMC) was procured from Sisco Research Laboratories, India. 1.

Physical, Spectral and Thermal Properties of Resorcinol Abstract Resorcinol is widely used in manufacturing of several drugs and pharmaceutical products that are mainly used for topical ailments. The main objective of this study is to use an alternative strategy i.e., biofield treatment to alter the physical, spectral and thermal properties of resorcinol. The resorcinol sample was divided in two groups, which served as control and treated group. The treated group was given biofield treatment and both groups i.e., control and treated were analysed using X-ray diffraction (XRD), Fourier transform-infrared (FT-IR) spectroscopy, UV-Visible (UVVis) spectroscopy, Differential scanning calorimetry (DSC) and Thermogravimetric analysis (TGA). Keywords: Resorcinol; Biofield energy treatment; X-Ray diffraction; Fourier transform infrared spectroscopy; Ultraviolet-Visible spectroscopy; Differential scanning calorimetry; Thermogravimetric analysis Abbreviations Introduction Materials and Methods Study design Resorcinol was procured from Loba Chemie Pvt. 1.

Energy Treatment Impact on Antifungal Sensitivity Assay Abstract Fungi are the group of eukaryotic organisms such as yeast, mold, and mushrooms. The present work investigated the impact of biofield treatment on different pathogenic species of fungi in relation to antifungal sensitivity pattern. Each fungal sample was divided into three parts: C, control; T1, treatment (revived); T2 treatment (lyophilized). Treatment groups received the biofield treatment, and control group was remained as untreated. Keywords: Fungi; Amphotericine B; Fluconazole; Flucytosine; Itraconazole Introduction The kingdom fungi includes yeast, mold, and mushrooms that have multiple applications like fermentation of sugar and bear; productions of antibiotics and enzymes; as biological pesticide; and also consumed as food products like mushrooms, morels, and truffles. Energy is the property of matter that neither can be created nor destroyed, however it can be transmitted to other substances by changing into different forms. Materials and Methods Biofield treatment Results

Quality of Beef Extract & Meat Infusion Powder- Energy Impact The present research work investigated the influence of bio-field treatment on two common flavoring agents used in food industries namely beef extract powder (BEP) and meat infusion powder (MIP). The treated powders were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), particle size analysis, surface area analysis, differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The FT-IR results showed disappearance of triglycerides peaks in both the treated powders as compared to control. XRD results corroborated the amorphous nature of both control and treated samples. Keywords : Beef extract powder; Meat infusion powder; Bio-field treatment; Fourier transform infrared spectroscopy; X-ray diffraction; Particle size analysis; Surface area analysis; Differential scanning calorimetry; Thermo gravimetric analysis Beef is known to have excellent nutritional value and it has been widely consumed in many countries. Mr. Discussion 1.