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Antimycobacterial Susceptibility Testing of Antimicrobials

Antimycobacterial Susceptibility Testing of Antimicrobials
Abstract The aim was to evaluate the impact of biofield treatment modality on mycobacterial strains in relation to antimycobacterials susceptibility. Mycobacterial sensitivity was analysed using 12 B BACTEC vials on the BACTEC 460 TB machine in 39 lab isolates (sputum samples) from stored stock cultures. Two American Type Culture Collection (ATCC) strains were also used to assess the minimum inhibitory concentration (MIC) of antimicrobials (Mycobacterium smegmatis 14468 and Mycobacterium tuberculosis 25177). Rifampicin, ethambutol and streptomycin in treated samples showed increased susceptibility as 3.33%, 3.33% and 400.6%, respectively, as compared to control in extensive drug resistance (XDR) strains. Keywords: Antimycobacterial susceptibility; Biofield treatment;MDR-TB; XDR-TB; BACTEC 460/MGIT-TB; MIC Introduction Global tuberculosis control is facing major challenges now days. Mr. Materials and Methods Biofield Treatment Strategy and Experimental Design Results and Discussion References

Providencia Rettgeri Biochemical Reactions Analysis Abstract Providencia rettgeri (P. rettgeri) is the key organism for gastrointestinal tract infections due to its high virulence properties. The current study was designed to investigate the effect of Mr. Trivedi’s biofield energy treatment on P. rettgeri in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. The lyophilized strain of P. rettgeri (ATCC 9250) was divided into two parts, Group (Gr.) I: control and Gr. Keywords: Providencia rettgeri, Antimicrobial Sensitivity, Minimum Inhibitory Concentration, Biofield Treatment,Biochemical Reaction, Biotype 1. Providencia rettgeri (P. rettgeri) is the key organism for gastrointestinal tract infections due to its high virulence properties. 2. The strain P. rettgeri, bearing the American Type Culture Collection (ATCC 9250) strain was procured from MicroBioLogics, Inc., USA. 2.1. Group I: ATCC strain in the lyophilized state was considered as control. 2.2. 2.3. 2.4.

Energy Treatment Impact on Antifungal Sensitivity Assay Abstract Fungi are the group of eukaryotic organisms such as yeast, mold, and mushrooms. The present work investigated the impact of biofield treatment on different pathogenic species of fungi in relation to antifungal sensitivity pattern. Each fungal sample was divided into three parts: C, control; T1, treatment (revived); T2 treatment (lyophilized). Keywords: Fungi; Amphotericine B; Fluconazole; Flucytosine; Itraconazole Introduction The kingdom fungi includes yeast, mold, and mushrooms that have multiple applications like fermentation of sugar and bear; productions of antibiotics and enzymes; as biological pesticide; and also consumed as food products like mushrooms, morels, and truffles. Energy is the property of matter that neither can be created nor destroyed, however it can be transmitted to other substances by changing into different forms. Materials and Methods Biofield treatment Investigation of antifungal sensitivity and fungal strain identification Results Antifungal sensitivity

Assessment of Shigella Boydii Characteristics Abstract Bacillary dysentery and acute gastroenteritis caused by infection of Shigella species are major public health burden in India and its neighboring countries. Emergence of antimicrobial resistance threatens to render current treatments ineffective. The current study was attempted to investigate the effect of biofield treatment on Shigella boydii (S. boydiii) with respect of antimicrobial susceptibility assay, biochemical characteristics and biotyping. The American Type Culture Collection (ATCC 9207) strain of S. boydiii was used in this experiment. Keywords: Shigella boydii, Antimicrobial Sensitivity, Biofield Treatment, Biochemical Reaction, Biotype, Bacillary Dysentery, Shigellosis, Acute Gastroenteritis 1. Shigella boydii (S. boydiii) is a non-motile, non-spore forming, non-lactose fermenting and Gram-negative rod shape bacterium that belongs to the family of Enterobacteriaceae. 2. 2.1. Two ATCC samples A (revived) and B (lyophilized) of S. boydiii were grouped (Gr.). 2.2. 3.

Quality of Beef Extract & Meat Infusion Powder- Energy Impact The present research work investigated the influence of bio-field treatment on two common flavoring agents used in food industries namely beef extract powder (BEP) and meat infusion powder (MIP). The treated powders were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), particle size analysis, surface area analysis, differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The FT-IR results showed disappearance of triglycerides peaks in both the treated powders as compared to control. Keywords : Beef extract powder; Meat infusion powder; Bio-field treatment; Fourier transform infrared spectroscopy; X-ray diffraction; Particle size analysis; Surface area analysis; Differential scanning calorimetry; Thermo gravimetric analysis Beef is known to have excellent nutritional value and it has been widely consumed in many countries. Coronory heart disease (CHD) is the main cause of death in western countries. Mr. Discussion Conclusion

Klebsiella Pneumoniae Biochemical Characteristics Abstract: Pathogenic isolates of Klebsiella pneumoniae (K. pneumoniae), particularly the extended-spectrum β-lactamase (ESBL) producing strains, are mostly associated with the failure of antibiotic therapy in nosocomial infections. The present work was designed to evaluate the impact of Mr. Trivedi’s biofield energy treatment on phenotypic and genotypic characteristics of K. pneumoniae. The strain of K. pneumoniae bearing ATCC 15380 (American Type Culture Collection) was procured from the Bangalore Genei, in sealed pack and divided into control and treated groups. Treated group was subjected to Mr. Keywords: Klebsiella pneumoniae; Biofield energy treatment; Antibiogram, Biochemical reactions, Polymorphism; Random Amplified Polymorphic DNA. Abbreviations: Introduction The increased medical practice for antibiotic usage creates selection pressure and results emergence of nosocomial pathogens. Materials and Methods Biofield treatment modalities Biochemical studies Biotype number Conclusion 1.

Gluten Hydrolysate - Physical and Thermal Properties Modification Abstract The objective of present study was to study the effect of biofield treatment on physical and thermal properties of gluten hydrolysate (GH) and ipomoea macroelements (IM). The study was performed in two groups (control and treated). The control group remained as untreated, and biofield treatment was given to treated group. The control and treated GH and IM were characterized by particle size analysis, surface area analysis, X-ray diffraction (XRD), Differential scanning calorimetry (DSC), and Thermogravimetric analysis (TGA). Particle size results of treated GH showed that d50 (average particle size) was decreased by 3.15% and d99 (size exhibited by 99% of powder particles) by 18.40% as compared to control GH sample. Keywords: Gluten hydrolysate; Ipomoea macroelements; Biofield treatment; Particle size; Surface area; XRD; DSC; TGA Introduction Experimental The gluten hydrolysate (GH) and ipomoea macroelements (IM) powder were procured from HiMedia Laboratories Pvt. Characterization

Study of Multidrug Resistant Strain of K. Oxytoca Abstract Klebsiella are opportunistic pathogens that cause a wide spectrum of severe diseases. The aim of the present study was to investigate the impact of biofield treatment on multidrug resistant strain of K. oxytoca with respect to antibiogram pattern along with biochemical study and biotype number. Clinical lab isolate of K. oxytoca was divided into two groups i.e. control and treated. Control group remain untreated and treated group was subjected to Mr. Keywords: Klebsiella oxytoca ; Multidrug resistant; Antibiogram; Biofield treatment; Biochemical reactions; Biotyping Introduction Klebsiella oxytoca (K. oxytoca) is a Gram-negative pathogen, cylindrical rod shaped, non-motile in nature, and belongs to Enterobacteriaceae family. Biofield is a cumulative outcome of electric and magnetic field energy, exerted by the human body. Materials and Methods Experimental design and biofield treatment Evaluation of antimicrobial susceptibility assay Biochemical reaction study Results and Discussion

Chloramphenicol & Tetracycline - Spectroscopic Analysis Abstract Objective: Chloramphenicol and tetracycline are broad-spectrum antibiotics and widely used against variety of microbial infections. Nowadays, several microbes have acquired resistance to chloramphenicol and tetracycline. The present study was aimed to evaluate the impact of biofield treatment for spectroscopic characterization of chloramphenicol and tetracycline using FT-IR and UV-Vis spectroscopy. Methods:The study was performed in two groups (control and treatment) of each antibiotic. Results: FT-IR spectrum of treated chloramphenicol exhibited the decrease in wavenumber of NO2 from 1521 cm-1 to 1512 cm-1 and increase in wavenumber of C=O from 1681 cm-1 to 1694 cm-1 in acylamino group. Conclusion: Based on FT-IR spectroscopic data, it is speculated that due to increase in bond strength and conjugation effect after biofield treatment, the chemical stability of both the drugs might be increased as compared to control. Introduction: Materials and Methods Study design Conclusion 1.

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