Phenotyping and Genotyping Characteristics of Serratia Marcescens
biochemical reactions. We also explored the genotyping of this organism using polymerase chain reaction (PCR) based methodologies of randomly amplied polymorphic DNA (RAPD) and 16S rDNA sequencing techniques. report that explores the impact of bioeld treatment on S. marcescens Materials and Methods Two vials of [American Type Culture Collection (ATCC) 13880] were procured from MicroBioLogics, Inc., USA, in sealed packs, and stored as per the recommended storage conditions until further use. reactions, and biotype number were evaluated on MicroScan Walk- Away® (Dade Behring Inc., West Sacramento, CA) using Negative Breakpoint Combo 30 (NBPC30). analysis (using Ultrapure Genomic DNA Prep Kit; Cat KT 83) and the 16S rDNA sequencing studies were carried out using Ultrapure Genomic DNA Prep Kit; Cat KT 83 (Bangalore Genei, India). tested antimicrobials, biochemicals and other reagents were procured from Sigma-Aldrich. Study design e microorganisms were grouped as per study design like bacterial control.
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