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Assessment of Pseudomonas Fluorescens Antibiotic Susceptibility

Assessment of Pseudomonas Fluorescens Antibiotic Susceptibility
Abstract Global emergence of Pseudomonas fluorescens (P. fluorescens) displays a mechanism of resistance to all existing antimicrobials. Due to its strong ability to acquire resistance, there is a need of some alternative treatment strategy. Objective of this study was to investigate the effect of biofield treatment on antimicrobial sensitivity pattern of P. fluorescens. P. fluorescens cells were procured from MicroBioLogics in sealed packs bearing the American Type Culture Collection (ATCC 49838) number. Two sets of ATCC samples were taken in this experiment and denoted as A and B. Keywords: Pseudomonas fluorescens; Antibiotic Susceptibility; Biofield Treatment; Biochemical Reactions; Biotyping Introduction Pseudomonas fluorescens (P. fluorescens) is Gram negative, obligate aerobic, and considered as a psychrotrophic microorganism, unable to grow at temperatures above 32°C. Biofield is the name given to the electromagnetic field that permeates and surrounds living organisms. Study Design Related:  Biofield Energy Treatment

Alteration in Escherichia Coli Antibiotic Sensitivity Pattern Abstract Study background: Multidrug resistant Escherichia coli (MDR E. coli) has become a major health concern, and failure of treatment leads to huge health burden. Aim of the present study was to determine the impact of Mr. Trivedi’s biofield treatment on E. coli. Methods: Four MDR clinical lab isolates (LSs) of E. coli (LS 8, LS 9, LS 10, and LS 11) were taken and divided into two groups i.e. control and biofield treated. Results: Antimicrobial sensitivity assay showed 50% alteration in sensitivity of total tested antimicrobials in treated group of MDR E. coli isolates. Conclusion: Overall results suggest that Mr Trivedi’s biofield treatment has a significant effect on altering the antimicrobial sensitivity, biochemical reactions and biotype number of MDR isolates of E. coli. Keywords: Escherichia coli; Biofield treatment; Multidrug-resistant; Antimicrobial susceptibility; Biochemical reaction; Biotyping Abbreviations: Introduction Material and Methods MDR clinical lab isolates (i.e. 1.

Biofield Treatment on Enterococcus faecalis | Trivedi Science There Are Lots Of Exciting Things Happening Here At Trivedi Science. Enter Your Email Address In The Box Below Now To Stay Updated! Enterococcus Faecalis [ATCC – 51299] Abstract Background: While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. Methods: Enterococcus faecalis strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mr. Results: The results indicated that Mr.Trivedi’s energy has changed 9 of 27 biochemical characteristics of Enterococcus faecalis along with significant changes in susceptibility pattern in 5 of 31 antibiotics. Conclusions: These results cannot be explained by current theories of science, and indicate a potency in Mr.Trivedi’s energy, providing a model for science to be able to investigate the impact of spiritual energy in a rigorous manner.

Biofield Treatment on Staphylococcus epidermis | Trivedi Science There Are Lots Of Exciting Things Happening Here At Trivedi Science. Enter Your Email Address In The Box Below Now To Stay Updated! Staphylococcus Epidermis [ATCC –13518] Abstract Purpose: While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. Methods: Staphylococcus epidermis strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mahendra Trivedi to the sealed tubes containing strain, the process taking about 3 minutes and were analyzed within 10 days after incubation. Results: The results indicated that Mahendra Trivedi energy has changed 7 of 27 biochemical characteristics of Staphylococcus epidermis along with significant changes in susceptibility pattern in 8 of 29 antibiotics. Conclusions: Agriculture Publications Biotechnology Publication Materials Science Publications

Characterization of Phenotype and Genotype of Biofield Treated Enterobacter aerogenes - Trivedi Science Abstract Enterobacter aerogenes (E. aerogenes) has been commonly described as a versatile opportunistic pathogen in hospital infections. The aim of the present work was to evaluate the impact of biofield treatment on E. aerogenes for its phenotypic and genotypic characteristics. E. aerogenes bearing ATCC 13048 (American Type Culture Collection) was procured from Bangalore Genei, in sealed pack and divided into control and treated groups. Keywords: Enterobacter aerogenes, Biofield treatment; Phenotyping, Polymorphism; RAPD; 16S rDNA analysis Introduction Enterobacter aerogenes (E. aerogenes) is a common organism of most of the hospital-acquired infections. Biofield is the name given to the electromagnetic field that permeates and surrounds living organisms [7]. After consideration of the clinical significance of E. aerogenes and significant impact of Mr. Study design and biofield treatment E. aerogenes strain was divided into two groups i.e. control and treatment. Results and Discussion 1.

Alteration in Biochemical Characteristics of Yersinia Enterocolitica Abstract Background: While spiritual and mental energies are known to man, their impact has never been scientifically measurable in the material world and they remain outside the domain of science. The present experiments on Yersinia enterocolitica [ATCC –23715], report the effects of such energy transmitted through a person, Mahendra Trivedi, which has produced an impact measurable in scientifically rigorous manner. Methods: Yersinia enterocolitica strains in revived and lyophilized state were subjected to spiritual energy transmitted through thought intervention and/or physical touch of Mahendra Trivedito the sealed tubes containing strain and were analyzed within 10 days after incubation. Results: The results indicated that Mahendra Trivedi‘s energy has changed 20 of 33 biochemical characteristics of Yersinia enterocolitica along with significant changes in susceptibility pattern in 15 of 32 antibiotics. Conclusions: Introduction Material and Methods Group I Treatment Group II MicroScan Setting

Antibiogram of Multidrug-Resistant Isolates of Pseudomonas aeruginosa after Biofield Treatment - Trivedi Science Abstract In recent years, prevalence of multidrug resistance (MDR) in Pseudomonas aeruginosa (P. aeruginosa) has been noticed with high morbidity and mortality. Aim of the present study was to determine the impact of Mr. Trivedi’s biofield treatment on MDR clinical lab isolates (LS) of P. aeruginosa. Five MDR clinical lab isolates (LS 22, LS 23, LS 38, LS 47, and LS 58) of P. aeruginosa were taken and divided into two groups i.e. control and biofield treated. Control and treated group were analyzed for antimicrobial susceptibility pattern, minimum inhibitory concentration (MIC), biochemical study and biotype number using MicroScan Walk-Away® system. Keywords: Pseudomonas aeruginosa; Biofield treatment; Multidrug-resistant; Antimicrobial susceptibility; Biochemical reaction; Biotyping Introduction Antimicrobial agents are widely used therapeutic option against infections caused by pathogenic microbes. Materials and Methods Experimental design and biofield treatment Biochemical study 1.

Evaluation of Biofield Modality on Viral Load of Hepatitis B and C Viruses | Trivedi Science Abstract Study background: Nowadays, hepatitis is a major challenge for clinical research, regulatory bodies, and clinicians who are trying to assess the more effectiveness of antiviral therapy against patients. Viral load count is the amount of particular viral DNA or RNA in a blood samples. It is one of the surrogate biomarker of hepatitis. High viral load indicates that the immune system is failed to fight against viruses. Method: The viral load assay was performed on stock human plasma samples of HBV and HCV before and after 7 days of biofield treatment using Roche COBAS® AMPLICOR analyzer according to manufacturer’s instructions. Result: The viral load of HBV DNA in infected plasma samples showed a significant alteration in the biofield treated group as compared to control. Keywords: Hepatitis B virus; Hepatitis C virus; Biofield treatment; Viral load; HBV DNA; HCV RNA Abbreviations Introduction Hepatitis C is single-stranded RNA virus belongs to Flaviviridae family. Figure 1 Reference

Biofield Treatment On Multidrug-Resistant | Trivedi Science Abstract Burkholderia cepacia (B. cepacia) is an opportunistic, Gram negative pathogen which causes infection mainly in immunocompromised population and associated with high rate of morbidity and mortality in cystic fibrosis patients. Aim of the present study was to analyze the impact of biofield treatment on multidrug resistant B. cepacia. Clinical sample of B. cepacia was divided into two groups i.e. control and biofield treated. Keywords: Burkholderia cepacia; Multidrug Resistant; Antimicrobial Susceptibility; Biofield Treatment; Biochemical Reactions; Biotyping Introduction Burkholderia cepacia (B. cepacia) is an important human pathogen, first isolated in cystic fibrosis patient and associated with serious health issues such as wound infection, bacteremia, catheter-related urinary infections and endocarditis [1]. During the last few decades, incidence of microbial resistance has increased which leads to generates multi-drug-resistance (MDR) organisms. Biochemical reaction study 1.

16S rDNA Analysis of Citrobacter Braakii Abstract Citrobacter braakii (C. braakii) is widespread in nature, mainly found in human urinary tract. The current study was attempted to investigate the effect of Mr. Trivedi’s biofield treatment on C. braakii in lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, and biotype number. Lyophilized vial of ATCC strain of C. braakii was divided into two parts, Group (Gr.) I: control and Gr. Keywords: Citrobacter braakii; Antimicrobial susceptibility; Biofield treatment; Biochemical reaction; Biotype; 16S rDNA analysis; Gramnegative bacteria; Enterobacteriaceae Introduction Citrobacter braakii (C. braakii) is a genus of Gram-negative, straight, facultative anaerobic and motile bacilli bacterium widely distributed in water, soil, and food in the environment. Materials and Methods Experimental design The impact of biofield treatment on tested bacterium C. braakii was evaluated in two groups- Group IIB – Study I Group IIB – Study II Gr.: Group

Antibiogram and Genotypic Analysis using 16S rDNA after Biofield Treatment on Morganella morganii - Trivedi Science Abstract Morganella morganii (M. morganii) is one of the important nosocomial pathogen associated with the urinary tract infections and bacteremia. The aim of this study was to evaluate the effect of Mr. Trivedi’s biofield energy treatment on M. morganii in the lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, biotype number and genotype. M. morganii cells were procured from MicroBioLogics Inc., USA in sealed packs bearing the American Type Culture Collection (ATCC 25829) number and stored according to the recommended storage protocols until needed for experiments. Keywords: Morganella morganii; Antimicrobial susceptibility; Biofield energy; Biochemical reaction; Biotype; 16S rDNA analysis Introduction Morganella species are the clinically characterized in the tribe Proteeae [1]. Biofield has been defined as “energy fields that purportedly surround and penetrate the human body”. Materials and Methods Inoculum preparation Conclusion

Evaluation of Phenotyping and Genotyping Characterization of Serratia marcescens after Biofield Treatment - Trivedi Science Abstract Serratia marcescens (S. marcescens) is Gram-negative bacterium, associated with hospital-acquired infections (HAIs), especially urinary tract and wound infections. The present study was aimed to evaluate the impact of biofield treatment on phenotyping and genotyping characteristics such as antimicrobial susceptibility, biochemical reactions, biotype, DNA polymorphism, and phylogenetic relationship of S. marcescens (ATCC 13880). The lyophilized cells of S. marcescens were divided into three groups (G1, G2, and G3). Keywords: Antimicrobials; Biofield treatment; Polymorphism; Microbial resistance; RAPD; S. marcescens Introduction Currently, many microorganisms have been acquired the resistance to number of antibiotics and other antimicrobial agents, which were effectively used earlier to cure a microbial infections. The relation between mass-energy was described Friedrich, then after Einstein gave the well-known equation E=mc2 for light and mass [4,5]. Materials and Methods Results

Antibiogram, Biochemical Reactions and Genotyping Characterization of Biofield Treated Staphylococcus aureus - Trivedi Science Abstract Staphylococcus aureus (S. aureus) is the key organism for food poisoning due to massive production of heat stable exotoxins. The current study was attempted to investigate the effect of Mr. Keywords: Staphylococci, Staphylococcus aureus, Antimicrobial Sensitivity, Biofield Treatment, Biochemical Reaction, Biotype, 16S rDNA, Gram-Positive Bacteria 1. Staphylococci are the important class of pyogenic Grampositive spherical bacteria resembling to the grapes like structure. National Institute of Health/National Center for Complementary and Alternative Medicine (NIH/NCCAM) have reported that biofield (putative energy fields) or electromagnetic based energy therapies used to promote health and healing [8]. 2. S. aureus, American Type Culture Collection (ATCC 25923) strain was procured from MicroBioLogics, Inc., USA and stored with proper storage conditions until further use. 2.1. The impact of biofield treatment on tested bacterium S. aureus was evaluated in two groups- 2.2. 2.3. 2.4.

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