Sequencing Primer Design. Primer3 Input (version 0.4.0) Copyright Notice and Disclaimer Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007 Whitehead Institute for Biomedical Research, Steve Rozen, Maido Remm, Triinu Koressaar and Helen Skaletsky All rights reserved.
Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met: * Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. * Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution. * Neither the names of the copyright holders nor contributors may be used to endorse or promote products derived from this software without specific prior written permission. Citing Primer3 We request that use of this software be cited in publications as Source code available at primer3.sourceforge.net/. T-DNA Primer Design. OligoCalc: Oligonucleotide Properties Calculator.
Thermodynamic Calculations taking into account base stacking energy The nearest neighbor and thermodynamic calculations are done essentially as described by Breslauer et al., (1986) Proc.
Nat. Acad. Sci.83:3746-50 (Abstract) but using the values published by Sugimoto et al., (1996) Nucl. Acids Res.24:4501-4505 (Abstract). Primer designing tool.