The Dawn of Virtual Cell Biology: journal club video available. Today I attended a virtual journal club.
Organized by Stephen Larson of OpenWorm (I talked about that very cool 3D worm project in a tip of the week a while back), it also included a few folks from OpenWorm, and two of the authors of the paper: Jonathan Karr and Jayodita Sanghvi. Jonathan’s cat also showed up at one point Stephen did a part in the beginning that was a presentation of the main features of the paper, and the context of the work in biology today.
I think he had quite a similar take on it to what I was describing in my tip this week: modeling types of papers cause a bit of drama and resistance in the biology community; and that this relied on so much previous work in hundreds of publications and lots of curated databases. But he also stressed the importance of this work in moving this field forward. The discussion part was terrific. Anyway, the video is available and you can watch. I really enjoyed the virtual journal club idea and format. Noncanonical Wnt Signaling Maintains Hematopoietic Stem Cells in the Niche. To view the full text, please login as a subscribed user or purchase a subscription.
Click here to view the full text on ScienceDirect. Nuclear Envelope Budding Enables Large Ribonucleoprotein Particle Export during Synaptic Wnt Signaling. Figure 1 Subnuclear Localization of DFz2C and LamC at Larval Muscle Nuclei and Defective NMJs in lamC Mutants (A) LamC and DFz2C labeling (deconvolved) of muscle nucleus containing a DFz2C/LamC focus (box; enlarged in right panels) localized to the nuclear periphery (arrowhead in XZ plane).
Arrows indicate DFz2C granule within the LamC framework-like structure. Structure - Visualizing the Determinants of Viral RNA Recognition by Innate Immune Sensor RIG-I. To view the full text, please login as a subscribed user or purchase a subscription.
Click here to view the full text on ScienceDirect. Figure 1. Snapshot: Endogenous RNAi Pathways. Redirection page. Transcriptional Silencing of Transposons by Piwi and Maelstrom and Its Impact on Chromatin State and Gene Expression. Structural Switch of Lysyl-tRNA Synthetase between Translation and Transcription. To view the full text, please login as a subscribed user or purchase a subscription.
Click here to view the full text on ScienceDirect. Figure 1 Structural Basis for Reserving LysRS in the MSCdirect (A and B) Two orthogonal views of the human LysRS:p38/AIMP2 complex structure. Sequence of p38/AIMP2 is color coded as in (C). (C) Sequence alignment of the N terminus of p38/AIMP2 from Drosophila to human. (D and E) The interface of the LysRS:p38/AIMP2 complex. (F) The functional LysRS:p38/AIMP2 complex. RNase H2-Initiated Ribonucleotide Excision Repair. To view the full text, please login as a subscribed user or purchase a subscription.
Click here to view the full text on ScienceDirect. Figure 1 Ribonucleotide Incorporation during DNA Synthesis (A) Primed RPA-coated SS-M13mp18 DNA was fully replicated by either Pol δ or Pol ε with PCNA under standard conditions, with or without rNTPs ( Experimental Procedures ). Daddy Issues: Paternal Effects on Phenotype. To view the full text, please login as a subscribed user or purchase a subscription.
Click here to view the full text on ScienceDirect. Figure 1 Potential Mechanisms Underlying Paternal Environmental Effects on Offspring Phenotype. Capturing the Cloud: UAP56 in Nuage Assembly and Function. Transcriptome-wide Regulation of Pre-mRNA Splicing and mRNA Localization by Muscleblind Proteins. To view the full text, please login as a subscribed user or purchase a subscription.
Click here to view the full text on ScienceDirect. Figure 1. Epigenetic Reprogramming and Small RNA Silencing of Transposable Elements in Pollen. To view the full text, please login as a subscribed user or purchase a subscription.
Click here to view the full text on ScienceDirect. Specialized piRNA Pathways Act in Germline and Somatic Tissues of the Drosophila Ovary. To view the full text, please login as a subscribed user or purchase a subscription.
Click here to view the full text on ScienceDirect. Figure 1 Maternal Deposition of piRNAs Defines Somatic and Germline piRNA Pathways. Poxviruses Deploy Genomic Accordions to Adapt Rapidly against Host Antiviral Defenses. To view the full text, please login as a subscribed user or purchase a subscription. Click here to view the full text on ScienceDirect. Figure 1.
The Drosophila HP1 Homolog Rhino Is Required for Transposon Silencing and piRNA Production by Dual-Strand Clusters. To view the full text, please login as a subscribed user or purchase a subscription. Click here to view the full text on ScienceDirect. Figure 1 DNA Damage Signaling in rhi Mutants (A–C) Mutations in mnk, which encodes the DNA damage signaling kinase Chk2, suppress the Gurken and Vasa protein localization defects in rhi mutants. (A) In a stage 9 wild-type oocyte, Grk (blue) is localized at the dorsal anterior cortex near the oocyte nucleus and Vas (red) is localized at the posterior cortex. (B) In rhi egg chambers, this localization pattern is lost, with Grk and Vas dispersed throughout the oocyte. Developmental Cell - The TDRD9-MIWI2 Complex Is Essential for piRNA-Mediated Retrotransposon Silencing in the Mouse Male Germline. Figure 4. Distinct Argonaute-Mediated 22G-RNA Pathways Direct Genome Surveillance in the C. elegans Germline.
To view the full text, please login as a subscribed user or purchase a subscription. Click here to view the full text on ScienceDirect. Figure 1 Hypomorphic Alleles of drh-3 Are RNAi Deficient and Temperature Sensitive (A) Schematic of the drh-3 gene structure. Top panel, conserved DExH and HELICc domains and drh-3 lesions; bottom panel, four missense alleles (indicated) map to the HELICc domain. TOR-tured Yeast Find a New Way to Stand the Heat. Should I Stay or Should I Go? Chromodomain Proteins Seal the Fate of Heterochromatic Transcripts in Fission Yeast. Protection from Feed-Forward Amplification in an Amplified RNAi Mechanism. To view the full text, please login as a subscribed user or purchase a subscription.
Click here to view the full text on ScienceDirect. The Loop Position of shRNAs and Pre-miRNAs Is Critical for the Accuracy of Dicer Processing In Vivo. Clocks, Metabolism, and the Epigenome. PIDD Death-Domain Phosphorylation by ATM Controls Prodeath versus Prosurvival PIDDosome Signaling. Figure 4 ATM Phosphorylates PIDD on T788 during CS Apoptosis. LincRNA-p21 Suppresses Target mRNA Translation. A CXCL1 Paracrine Network Links Cancer Chemoresistance and Metastasis. Figure S2 CXCL1/2 Inhibition in Cancer Cells Does Not Affect Tumor Angiogenesis or Proliferation but Affects Myeloid Recruitment, Related to Figure 2 . Fluorescence-Based Sensors to Monitor Localization and Functions of Linear and K63-Linked Ubiquitin Chains in Cells. Discrete Small RNA-Generating Loci as Master Regulators of Transposon Activity in Drosophila. Figure 1. Structural and Mechanistic Basis for the Inhibition of Escherichia coli RNA Polymerase by T7 Gp2.
<div class="jsDisabled"> To use the Enhanced view of this article, please enable JavaScript on in your browser and refresh the page. </div> Article Search for articles by this author. Promoters Recognized by Forkhead Proteins Exist for Individual 21U-RNAs. Transcript Dynamics of Proinflammatory Genes Revealed by Sequence Analysis of Subcellular RNA Fractions. Redirection page. U1 snRNP Determines mRNA Length and Regulates Isoform Expression. Current Biology - Slicing-Independent RISC Activation Requires the Argonaute PAZ Domain. Direct Regulation of tRNA and 5S rRNA Gene Transcription by Polo-like Kinase 1.
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