Sebastian Kurscheid: the pitfalls of HTS data a... MyBioTechniques: Effective DNA/RNA Co-Extra... BioPortfolio: PolyPid Joins Rimonim Cons... Twitter. Bio-Network: Genom... Life Sciences: Genom... Silence Journal: Alnylam Appoints Harvard P... Biology Questions: What are the roles of pent... Bio_Network : Upreg... Life_Sciences_ : Upreg... Bio_Network : Getti... Getting RNA and Protein in Phase. Life_Sciences_ : Getti... Bio_Network : Polyv... Anamika13 : The benzamide M344, a nove... Life_Sciences_ : Rotav... Anamika13 : Long non-coding RNAs: vers... Bio_Network : Rotav... Differentiation of biologically distinct Cucumber mosaic isolates by double stranded RNA profile | and B.K. GIRl | Indian Phytopathology.
Differentiation of biologically distinct Cucumber mosaic isolates by double stranded RNA profile SHELLY PRAVEEN, SAVARNI TRlPATHI and B.K. GIRl Abstract Four isolates of Cucumber mosaic virus from Nicotiana tabacum, Commellina species and Petunia, designated as MV- T, C, PI and P 2 , differed in their host reaction, temperature sensitivity, and at the molecular level. Serologically related isolates of CMV viz. CMV-P I , P 2 , C and T were analyzed on the basis of their symptomology, temperature tolerance and double stranded RNA pattern. Double stranded RNA (ds-RNA) pattern of these CMV isolates representing biological diversity were examined. Genomigence: NAR: {beta}-Catenin recogn... Michael W. Pfaffl: Modification of mRNA expre... Bio-Network: Effec... Life Sciences: Effec... Shrikant Mantri: Scripps Gets NIH Funding f... RNAsecrets : RT “@Ninja_Kangaroo: Dis... Daniel Sprockett: Boiling Springs Lake RNA-D... GEN: Fusion Protein Effectively... PhysOrg Biology News: Molecular alternatives to...
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Because a function of RNA is to be translated as the genetic instructions for the protein-making machinery of cells, RNA editing is the body's way of fine-tuning the proteins it produces, allowing us to adapt. The enzyme ADAR, which does this editing job in the nervous system of creatures ranging from mice to men, even edits itself. In a new study that examined the self-editing process and locked it down at two extremes in fruit flies, Brown University scientists found some surprising insights into how this "fine-tuning of the fine-tuner" happens, including bizarre behavioral effects that come about when the self-editor can't edit. Take mating, for instance. When the researchers fixed self-editing in dADAR (as drosophila fruit fly ADAR is known) at a very high level in males, they created fruit fly wallflowers.
By contrast, when they fixed self-editing at a low level, they created shameless pick-up artists. The inner workings "Auto-editing changes with temperature," Reenan said. LifeTechSupport: Training Course: PGM™ RNA... GEN: Use of #siRNA in Therapeut... Use of siRNA in Therapeutic Arena on the Upswing. In contrast to miRNAs, siRNAs are usually perfectly complementary to their targets. They are thus very effective at eliminating gene expression.
For this reason, synthetic siRNAs have been generated for a number of therapeutic uses. But of course, there are hurdles: stability, potency, off-target effects, and efficient delivery of synthetic siRNAs are some of the major challenges for successful application of this technology in the clinic. Quark Pharmaceuticals is committed exclusively to developing siRNA drugs. One of its siRNAs has finished Phase II trials for the treatment of wet age-related macular degeneration and diabetic macular edema. This siRNA was licensed to Pfizer, which conducted the trials. The diabetic macular edema trial was reportedly the first siRNA Phase II trial to show dose-dependent efficacy—there was an actual and significant improvement in clinical endpoint (visual acuity), not just a knockdown of gene expression. Both siRNAs treat eye diseases; Dr. Dr. MicrobeWorld: RNA virus packaging specif...
BioTechniques: Hybrid RNA-DNA Virus Disco... BioTechniques Daily Newsletter - Microscopy > Multi-Label Techniques for Colocalization StudieS User protocol for multi-color immunohistochemistry staining in intact tissue Double-staining and even triple-staining with poly- or monoclonal antibodies in immunohistochemistry (IHC) can enhance the study of colocalization—the presence of two or more antigens in one cell. Conventional methods for imaging colocalization, however, are prohibitive because overlapping colors are often indiscernible. These generally applicable IHC staining methods can be widely applied in the biological sciences. CRi’s Nuance™ multispectral imaging system allows separation and subsequent quantitation of each antigen, including the counterstain, as well as autofluorescence removal in immunofluorescence (IF). Next-generation sequencing Power Next-Generation Sequencing with Innovative Sample Preparation Solutions from NuGEN®
Invitrogen: Researchers discover natur... Hot spring yields hybrid genome. In the hostile environment of a bubbling volcanic hot spring, a team of researchers at Portland State University in Oregon has discovered a new viral genome that seems to be the product of recombination between a DNA virus and an RNA virus — a natural chimaera not seen before. Their findings appeared on 19 April in the journal Biology Direct. “It’s a mythological beast of a virus, but it actually exists,” says virologist Ken Stedman, who coauthored the study. Image via Wikipedia under Creative Commons Boiling Springs Lake is home to a bizarre DNA-RNA hybrid virus.
In the bacterial communities that populate the acidic waters of Boiling Springs Lake in northern California’s Lassen Volcanic National Park, “viruses are the only predators”, Stedman says. To get a better handle on what types of viruses are present there, he and his colleagues performed a metagenomic analysis of hundreds of thousands of viral sequences from a lake sample.
Stedman suggests two possible explanations. MyBioTechniques: Hybrid RNA-DNA Virus Ident... Hybrid RNA-DNA Virus Identified. Hybrid RNA-DNA Virus Identified Diana Gitig, Ph.D. While doing fieldwork at Boiling Springs Lake in California, researchers have found a rather unusual genome, the result of recombination between DNA and RNA viruses. Viruses mutate and trade genes all the time, which serves them really well but has not been so great for us. In addition to making it that much trickier for our immune systems to combat them, it has also made it much more difficult to study their evolution and to categorize them taxonomically. While doing fieldwork at Boiling Springs Lake in California, researchers have found a rather unusual genome, the result of recombination between DNA and RNA viruses. Geoffrey Diemer and Kenneth Stedman are researchers at the Center for Life in Extreme Environments at Portland State University, so it was hardly surprising when they found themselves doing fieldwork at Boiling Springs Lake in Lassen Volcanic Park, which is located in northeastern California.
References. Twitter. GenomeBiology: David Adams & @sangerinsti... Genome Engineering: Transcription initiation f... Sebastian Kurscheid: Small... ASBMB: Come hear Elisabetta Ullu... Gene Silencing News: Don't overlook the major i... ASBMB: The "team showed that the... BioPortfolio: BioPortfolio News Mmi1 RNA... Eureka Biology: Synergistic actions betwee... RNA-Mediated Silencing Mechanisms in Mammalian Cells - Life Sciences Social Network. Eureka Biology: RNA-Mediated Silencing Mec... Bio-Network: RNA Templating of Molecula... Eureka Biology: MTR4, a putative RNA helic... Bio-Network: MTR4, a putative RNA helic... WIREs RNA: Magnetic Field Sensed by G... Bio-Network: Messenger RNA processing a... Bio-Network: Optimization of RNA isolat... BioPortfolio: BioPortfolio News Mirna Th... EMD Millipore Bio: New RIPAb+ #antibody for W... Eureka Biology: The role of TFAM-associate... Cshperspectives: New issue live, inc. Rob M... Genomeresearch: Genome Research September...
WIREs RNA: Biogenesis of spliceosomal... Delivery of RNAi mediators. Focus Article Lance P. Ford, Masoud M. Toloue Published Online: Jun 03 2010 DOI: 10.1002/wrna.12 <h3>Abstract</h3> Delivering polynucleotides into animals has been a major challenge facing their success as therapeutic agents. <h3 class="article-tab-heading">Images</h3> This WIREs title offers downloadable PowerPoint presentations of figures for non-profit, educational use, provided the content is not modified and full credit is given to the author and publication. Download a PowerPoint presentation of all images Figure 1. The analysis of knockdown of GAPDH in SKBR3 or MCF7 xenograft tumor cells using receptor‐mediated siRNA delivery. . [ Normal View | Magnified View ] 1 Fire, A, Xu, S, Montgomery, MK, Kostas, SA, Driver, SE, Mello, CC. WIREs RNA: Delivery of RNAi mediators... WIREs RNA: Structural biology of poly... WIREs RNA: Techniques for following t...
WIREs RNA: Antibiotics that target pr... TS-Si News Service: "Scientists Override Genet... Eureka Biology: Genome-wide detection of n... BioPortfolio: BioPortfolio News IDTs new... The RNomics Daily. Eureka Biology: C-T variant in a miRNA tar... BioTechniques: Controversial RNAi Paper R... RNA preservation of Antarctic marine invertebrates - Life Sciences Social Network. Eureka Biology: RNA preservation of Antarc... Bio-Network: RNA preservation of Antarc... Genome Engineering: GEN | News Highlights:Fusi... BioInfo: High-Throughput Multiplex... High-Throughput Multiplex Sequencing o... [Curr Protoc Hum Genet. 2012. Silence Journal: Evolution seen in syntheti... BioCision: Have a good weekend, every... BioCision: Novel virus seems to be th... Laboratory News: Grant for researchers to d... Bio-Network: High-throughput RNAi Scree... Nature Reviews Micro: Highlight: Plant virology:...
Evolution seen in 'synthetic DNA' 19 April 2012Last updated at 14:07 ET Molecules called polymerases help to faithfully copy the genetic information stored in single strands of DNA Researchers have succeeded in mimicking the chemistry of life in synthetic versions of DNA and RNA molecules. The work shows that DNA and its chemical cousin RNA are not unique in their ability to encode information and to pass it on through heredity. The work, reported in Science, is promising for future "synthetic biology" and biotechnology efforts. It also hints at the idea that if life exists elsewhere, it could be bound by evolution but not by similar chemistry. In fact, one reason to mimic the functions of DNA and RNA - which helps cells to manufacture proteins - is to determine how they came about at the dawn of life on Earth; many scientists believe that RNA arose first but was preceded by a simpler molecule that performed the same function. 'No Goldilocks' Continue reading the main story “Start Quote.
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