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Introduction to Immobilized Lectin Affinity Con A Sepharose™ 4B Concanavalin A (Con A) binds molecules that contain a -D-mannose, a -D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups. Con A coupled to Sepharose™ 4B via cyanogen bromide activation.
In real-time PCR using SYBR green binding to amplified cDNA, we are simply measuring the fluorescence increase as the dye binds to the increasing amount of DNA in the reaction tube. We hope that this increase in fluorescence is coming from the DNA that we wish to measure but some of the signal could come from DNA other than that which we are trying to amplify. Is there any way to check that the correct fragments were amplified? One way to do some checking of the products is to do a melting curve.
Capillary Electrophoresis Primer As part of our commitment to the advancement of capillary electrophoresis, the following primer is provided to students and scientists who wish to become familiar with and learn about HPCE. Basic Capillary Electrophoresis Theory & Introduction Analytical Validation Glossary How to Properly Cut a Capillary : Animation on Best
Julian Downward , principal scientist (firstname.lastname@example.org) 1 Author Affiliations Introduction Over the past decade “RNA interference” has emerged as a natural mechanism for silencing gene expression. This ancient cellular antiviral response can be harnessed to allow specific inhibition of the function of any chosen target genes, including those involved in causing diseases such as cancer, AIDS, and hepatitis. RNA interference is already proving to be an invaluable research tool, allowing much more rapid characterisation of the function of known genes.
RNA interference (RNAi) is a mechanism that inhibits or activates gene expression at the stage of translation or by hindering the transcription of specific genes. RNAi targets include RNA from viruses and transposons (a form of innate immune response), and also plays a role in regulating development and genome maintenance. Small interfering RNA strands (siRNA) are key to the RNAi process, and have complementary nucleotide sequences to the targeted RNA strand.
<object classid="clsid:D27CDB6E-AE6D-11cf-96B8-444553540000" codebase="http://download.macromedia.com/pub/shockwave/cabs/flash/swflash.cab#version=9,0,28,0" width="920" height="518" title="scale"><param name="movie" value="/content/begin/cells/scale/Scale.swf" /><param name="FlashVars" value="mydate=2519" /><param name="quality" value="high" /><embed src="/content/begin/cells/scale/Scale.swf" quality="high" pluginspage="http://www.adobe.com/shockwave/download/download.cgi?P1_Prod_Version=ShockwaveFlash" type="application/x-shockwave-flash" width="920" height="518"></embed></object> Some cells are visible to the unaided eye The smallest objects that the unaided human eye can see are about 0.1 mm long. That means that under the right conditions, you might be able to see an ameoba proteus, a human egg, and a paramecium without using magnification. A magnifying glass can help you to see them more clearly, but they will still look tiny.
Biochemistry of Metabolism Instructional materials for a studio-format course * Information for Students * including needed software Note: I am retiring from teaching at Rensselaer. These educational materials will be kept on the Web for a time, but I am no longer updating the scientific content. Joyce Diwan