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荧光标记技术在蛋白质定位及功能研究中的应用. 随着分子生物学、有机化学以及材料科学等学科的进展,最近我们又获得了好几种新型的荧光蛋白标签,这些标签可以用于细胞生物学成像研究。

荧光标记技术在蛋白质定位及功能研究中的应用

本文将对荧光标志物在蛋白质研究中的优势及劣势进行一番详细的介绍,文章中将重点介绍如何使用荧光标志物研究活体细胞(而不是固定细胞)中的靶蛋白。 使用该方法可以对靶蛋白的表达情况、细胞中的定位情况、活性状态等指标进行研究,还将介绍将荧光显微镜与电子显微镜技术相结合的可行性问题。 小分子荧光标志物染料、纳米晶体材料,即所谓的“量子点(quantum dots)”材料、自发荧光蛋白、小分子蛋白质标签等等这些材料都可以作为荧光标志物,而且将这几种材料“混合”起来是一种非常有前途的荧光标志物研究新思路。 Handbook of biological confocal ... - Google 图书. Fluorochrome Table. Fluorophore. A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation.

Fluorophore

Fluorophores typically contain several combined aromatic groups, or plane or cyclic molecules with several π bonds. Fluorophores are sometimes used alone, as a tracer in fluids, as a dye for staining of certain structures, as a substrate of enzymes, or as a probe or indicator (when its fluorescence is affected by environment such as polarity, ions,...). But more generally it is covalently bonded to a macromolecule, serving as a marker (or dye, or tag, or reporter) for affine or bioactive reagents (antibodies, peptides, nucleic acids).

Fluorophores are notably used to stain tissues, cells, or materials in a variety of analytical methods, i.e., fluorescent imaging and spectroscopy.