Rosetta Genomics Receives 25th U.S. Patent Allowance. PHILADELPHIA and REHOVOT, Israel, March 18, 2013 /PRNewswire/ – Rosetta Genomics Ltd. (NASDAQ: ROSG), a leading developer and provider of microRNA-based molecular diagnostics, announces receipt of a Notice of Allowance from the U.S. Patent Office related to U.S. Patent Application No. 12/517,760 entitled “Nucleic Acids Involved in Viral Infection.” The allowed claims include a method of reducing the amount of virus replication in a target cell infected with the virus by introducing into the target cell an effective amount of miR-210, its variants or its precursor.
“We are delighted to add this new patent to our expanding intellectual property portfolio of microRNAs in viral infection. This newly granted patent further solidifies our dominant patent position in microRNAs and opens up other disease states where microRNAs may be of great benefit. Rosetta Genomics maintains a robust intellectual property strategy to protect its leadership position in microRNA technology. Differential miRNA expression profiles in proliferating or differentiated keratinocytes in response to gamma irradiation. Research article Nicolas Joly-Tonetti12, José Viñuelas12, Olivier Gandrillon12 and Jérôme Lamartine12* * Corresponding author: Jérôme Lamartine Jerome.lamartine@univ-lyon1.fr Author Affiliations 1 Université de Lyon, Université Claude Bernard Lyon I, Lyon, F-69003, France 2 CNRS, UMR5534, Centre de Génétique et de Physiologie Moléculaires et Cellulaires, Villeurbanne, F-69622, France For all author emails, please log on.
BMC Genomics 2013, 14:184 doi:10.1186/1471-2164-14-184 Published: 16 March 2013 Abstract Background MicroRNAs (miRNAs), a group of short non-coding RNAs that negatively regulate gene expression, have recently emerged as potential modulators of cellular response to ionizing radiations both in vitro and in vivo in various cell types and tissues. Results We settled up an expression study of miRNAs in primary human skin keratinocytes using a microfluidic system of qPCR assay, which permits to assess the expression of almost 700 annotated miRNAs.
Conclusions Keywords: close. RAmiRNA - Viral mature MicroRNA prediction Toolkit. MiRConnect 2.0: identification of oncogenic, antagonistic miRNA families in three human cancers. Bioinformatics - Batch convert miRNA names to Accession IDs? - Biology Beta - Stack Exchange. Current community your communities Sign up or log in to customize your list. more stack exchange communities Stack Exchange sign up log in tour help Biology beta Ask Question Take the 2-minute tour × Biology Stack Exchange is a question and answer site for biology researchers, academics, and students. Batch convert miRNA names to Accession IDs? 2 Answers active oldest votes Your Answer Sign up or log in Sign up using Google Sign up using Facebook Sign up using Stack Exchange Post as a guest discard By posting your answer, you agree to the privacy policy and terms of service. Not the answer you're looking for? Get the weekly newsletter!
Top questions and answers Important announcements Unanswered questions see an example newsletter By subscribing, you agree to the privacy policy and terms of service. Related Are there tools for automatically parsing glycan names into tree structures? Hot Network Questions question feed. Assessing the quality of miRNA rnaseq data. 13 months ago by Memphis Hi, I have got mouse miRNA rnaseq data and I am supposed to give my feedback about the quality of the library that was being developed for this project.
The barcodes are already trimmed off and the final reads range between 20bp to 70bp. I have grouped reads into two - one with read length between 20 to 35 bp (mature miRNAs) and the other greater than 35 bp (progenitors). Here is what I am planning to do a) Estimating the percentage of reads belonging to real miRNA (based on currently available annotation). I will be aligning the read sequences against already known mouse miRNA hairpins and mature/mature star miRNA sequences downloaded from miRBase. B) Calculating the complexity of the library.
As miRNAs are short entities and a particular type of miRNA can bind to 3' UTR of many genes I am not sure how to do this. Any other suggestions or link to relevant papers are also appreciated. Thanks. Bioinformatics Answers. ComiR - Combinatorial miRNA target prediction tool. My Biosoftware. Current Opinion in Genetics & Development - miRNA profiling of cancer. Volume 23, Issue 1, February 2013, Pages 3–11 Cancer genomics Edited By Nahum Sonenberg and Nissim Hay A steadily growing number of studies have shown that microRNAs have key roles in the regulation of cellular processes and that their dysregulation is essential to keep the malignant phenotype of cancer cells. The distorted and unique expression profile of microRNAs in different types and subsets of tumor coupled with their presence in biological fluids make of microRNAs an attractive source of sensitive biomarkers. Copyright © 2013 Elsevier Ltd. MicroRNA-145 Targeting in the Treatment of Pulmonary Arterial Hypertension.
IP generated as a result of collaboration between researchers from miRagen, the University of Glasgow and the University of Cambridge BOULDER, Colo., Mar 05, 2013 (BUSINESS WIRE) — –Preliminary results in pre-clinical models show significant promise in treatment of a disease with very high unmet medical need miRagen Therapeutics, Inc., a biopharmaceutical company developing innovative microRNA (miRNA)-based therapeutics, and the University of Glasgow, a world-leader in cardiovascular research, announced today that they have signed an exclusive license agreement for intellectual property they developed as part of an ongoing collaboration for microRNA-145 in pulmonary arterial hypertension (PAH).
This licensing agreement provides miRagen with exclusive rights to an important discovery made in collaboration with the University of Glasgow and the University of Cambridge, enabling miRagen to advance miR-145 inhibition as a potential therapeutic approach for PAH. About the University of Glasgow. Abstract | HuntMi: an efficient and taxon-specific approach in pre-miRNA identification. Methodology article Adam Gudyś1*, Michał Wojciech Szcześniak2*, Marek Sikora13 and Izabela Makałowska2 * Corresponding authors: Adam Gudyś adam.gudys@polsl.pl - Michał W Szcześniak miszcz@amu.edu.pl Author Affiliations 1 Institute of Informatics, Faculty Of Automatic Control, Electronics And Computer Science, Silesian University of Technology, Gliwice, Poland 2 Laboratory of Bioinformatics, Faculty of Biology, Adam Mickiewicz University, Poznan, Poland 3 Institute of Innovative Technologies EMAG, Katowice, Poland For all author emails, please log on.
BMC Bioinformatics 2013, 14:83 doi:10.1186/1471-2105-14-83 Published: 5 March 2013 Abstract Background Machine learning techniques are known to be a powerful way of distinguishing microRNA hairpins from pseudo hairpins and have been applied in a number of recognised miRNA search tools. Results We present HuntMi, a stand-alone machine learning miRNA classification tool. Conclusions Keywords: MicroRNA; Random forest; Imbalanced learning; Genome analysis. Stromal cell-induced miRNA alteration in chronic lymphocytic leukemia: how a minute and unavoidable cell contamination impairs miRNA profiling. Comparison of Analysis Tools for miRNA High Throughput Sequencing Using Nerve Crush as a Model. 1Collaborative Bioinformatics Center, Translational Genomics Research Institute, Phoenix, AZ, USA 2Neurogenomics, Translational Genomics Research Institute, Phoenix, AZ, USA 3Medical School, University of California San Francisco, San Francisco, CA, USA Recent advances in sample preparation and analysis for next generation sequencing have made it possible to profile and discover new miRNAs in a high throughput manner.
In the case of neurological disease and injury, these types of experiments have been more limited. Possibly because tissues such as the brain and spinal cord are inaccessible for direct sampling in living patients, and indirect sampling of blood and cerebrospinal fluid are affected by low amounts of RNA. We used a mouse model to examine changes in miRNA expression in response to acute nerve crush.
We assayed miRNA from both muscle tissue and blood plasma. Keywords: miRNA, small RNA, nerve injury, analysis, next generation sequencing, plasma, muscle Edited by: Reviewed by: An HNF4α-miRNA Inflammatory Feedback Circuit Regulates Hepatocellular Oncogenesis. (Cell 151, 1233–1247; December 9, 2011) In the above article, the Figure 6B and S7 legends included typographical errors that we would like to correct.
The tumor size was measured in mm and not in mm3, as indicated in the y axis of the figures, and the data are presented as mean ±SEM and not as mean ±SD. In addition, during the preparation of figures, we inadvertently inserted two images of the same tissue in Figure 8BFigure 8B (miR-124 staining in normal tissues, left and right panels). For a better representation of our findings, we provide a larger panel of images below. Black boxes indicate tissues that do not follow the pattern of the circuit (HNF4α/miR-124/STAT3/miR-24). Figure 8B HNF4α Circuit Is Perturbed during HCC Progression.
Transfection of siRNAs can alter miRNA ... [Biochim Biophys Acta. 2013. Study Sees Cigarette Smoke-Related Epigenetic and miRNA Links in Lung Cancer | GenomeWeb Daily News | PCR/Sample Prep. New Release: The UEA Small RNA Workbench Version 3.0 (Alpha) The UEA sRNA Workbench is a collection of software tools specifically designed to identify and analyze features in small RNA data. Yesterday, the Alpha release of The UEA Small RNA Workbench Version 3.0 was announced. This release includes a brand new tool for general small RNA locus detection called CoLIde. For more information and some changes to existing tools please see the following page for further details.
A stable release can be expected as soon as possible and will include further changes to other areas of the Workbench. When the stable release is available the final version code of the software will change to 3.0 Download 3.0 (Alpha) For the less adventurous: latest stable release is version 2.5.0 (Dec. 13th 2012) which can be found here: Download 2.5.0 (Stable Release) Tagged as: microrna, mirna, RNA Workbench.
Rosetta Genomics Receives Two Key U.S. Patent Allowances. NOTE: hsa-miR-29c* mentioned bellow is the old name for hsa-miR-29c-5p. PHILADELPHIA and REHOVOT, Israel, Feb. 14, 2013 /PRNewswire/ – Rosetta Genomics Ltd. (NASDAQ: ROSG), a leading developer and provider of microRNA-based molecular diagnostics, today announced that the Company has received two notices of allowance from the U.S. Patent and Trademark Office (“USPTO”) for Patent Applications 12/661,041 and 12/850,091. The allowed claims of 12/661,041, entitled “microRNAs and Uses Thereof,” cover the composition of matter for miR-29c*, as well as sequence variants (90% identical thereto), a vector and a probe comprising the same. miR-29c* is a core element in the Company’s lead commercial product, miRview® mets2 diagnostic assay for the identification of the tumor of origin in Cancer of Unknown and Uncertain Primary (“CUP”).
Rosetta Genomics maintains a robust intellectual property strategy to protect its leadership position in microRNA technology. SOURCE Rosetta Genomics Ltd. Firefly BioWorks Announces New Grant for microRNA Research. Dr. Davide Marini, co-founder and Chief Executive Officer of Firefly BioWorks announced today the creation of the Firefly Frontiers Grant: “We are excited to provide financial support to researchers for expanding the horizons of microRNA research. We encourage applicants to explore high-impact questions and those high-risk explorations that would not be normally funded through a traditional academic route”.
The Grant The Firefly Frontiers Grant is a $10,000 award in products and services to support exploratory studies in the area of microRNAresearch. Criteria Grant award decisions will be based on the quality, significance, and innovation of the proposed work, as well as on how the work may be enhanced by the use of the Firefly platform. How to apply Applications may be submitted online at Application deadline is March 29, 2013. Tagged as: Firefly BioWorks, grant, microrna, microrna research, mirna. Global profiling of miRNAs and the hairpin precursors: insights into miRNA processing and novel miRNA discovery. MiRNAs constitute an important class of small non-coding RNAs that regulate gene expression at the post-transcriptional level through sequence-specific base pairing (1). Most miRNAs are transcribed by the RNA polymerase II to generate primary miRNA (pri-miRNA) transcripts.
For canonical miRNAs, the pri-miRNAs bearing one or more imperfect inverted repeats are cleaved by the RNase III enzyme Drosha to yield pre-miRNAs hairpins (2,3). Alternatively, pre-miRNAs can be generated from debranched short introns with hairpin-forming potential (mirtron) by the spliceosome complex (4–9), or can be derived from other small non-coding RNAs such as snoRNAs (9–16). After being transported into the cytoplasm by the exportin-5 complex (17,18), pre-miRNAs are further processed by another RNase III enzyme Dicer (19) into double-stranded miRNA:miRNA* duplexes, of which one strand is incorporated into the RNA-induced silencing complex (RISC) (20–22) and guides the effector complex to the target mRNA (1). Study identifies miR-27b as liver gene that regulates cholesterol and fat blood levels. Researchers have identified a microRNA liver gene, miR-27b, which regulates lipid (cholesterol or fat) levels in the blood.
This regulator gene controls multiple genes involved in dyslipidemia—abnormal blood cholesterol levels that can contribute to heart disease. Study details published in the February issue of Hepatology, a journal of the American Association for the Study of Liver Diseases (AASLD), describe a new in silico approach to identify the significance of microRNAs in regulating disease-related gene pathways. The Human Genome Project (HGP) was completed in April, 2003 and the world had a map of the 3 billion DNA letters making up the human genome.
One of the HGP leaders was Dr. For the present study, lead author Dr. “We found liver miR-27b levels to be sensitive to high triglycerides (hyperlipidemia) in the blood and liver,” said Dr. The senior author of the study, Dr. In a related editorial published in this month’s issue of Hepatology, Dr. SOURCE: Review – Virus-Encoded microRNAs. New Open Access Journal OncomiRs Publishes First Research Paper. OncomiRs is a new international, peer-reviewed, open-access journal that welcomes research relevant to the interdisciplinary nature of microRNAs and cancer biology. The journal publishes original research articles and reviews covering all the basics aspects of the microRNAs in tumor formation and development as well as their role in the treatment sensitivity and resistance. OncomiRs focuses on the basic principles of microRNAs in the regulation of cellular processes such as apoptosis, cell cycle control, metastatic dissemination, cellular senescence and immortalization.
A particular attention is given to microRNA-controlled regulation of cellular oncogenes and tumor suppressor genes. Submitting to OncomiRs gives a unique chance to highlight findings in the only specialized microRNA and cancer research journal. Moreover, due to its open access model of publication, manuscripts accepted for publication are immediately published on-line. For the full article see: MicroRNA-182 Involved in Memory Formation. MicroRNA-210 – New Hope for Patients with Spinal Cord Injuries. Researchers identify and test first blood-based circulating miRNA biomarkers for Parkinson's. Analysis of CDS-located miRNA target sites suggests that they can effectively inhibit translation. Derepression of a Neuronal Inhibitor due to miRNA Dysregulation in a Schizophrenia-Related Microdeletion.
Current Opinion in Cell Biology - Regulation of miRNA biogenesis as an integrated component of growth factor signaling. Degradome Sequencing Workshop. MiRNA - Division of Genomics and RNomics, Biocenter Innsbruck ... NIH Awards Nearly $800K in Cancer-Related miRNA Grants in January. MicroRNomics: a newly emerging approach for disease biology. Evolutionary relationships between miRNA genes and their activity. Circulomics Developing miRNA Profiling Kits to Fill Multiplex Analysis Niche. MiRDeep*: an integrated application tool for miRNA identification from RNA sequencing data. MicroRNAs present exciting opportunities for cancer therapy and diagnosis. DLKI-DIO3 miRNA cluster suppresses spread of cancer from its primary site. Embryonic Priming of a miRNA Locus Predetermines Postmitotic Neuronal Left/Right Asymmetry in C. elegans. Job Listings. Small RNA deep sequencing reveals a distinct miRNA signature released in exosomes from prion-infected neuronal cells.
MirSNP, a database of polymorphisms altering miRNA target sites, identifies miRNA-related SNPs in GWAS SNPs and eQTLs. Integrated miRNA, mRNA and protein expression analysis reveals the role of post-transcriptional regulation in controlling CHO cell growth rate. MMpred: functional miRNA - mRNA interaction analyses by miRNA expression prediction. Selective autophagy degrades DICER and AGO2 and regulates miRNA activity : Nature Cell Biology. MicroRNAs in plants: Regulation of the regulator. A miRNA signature in leukocytes from sporadic amyotroph... [Gene. 2012. Fast-Forward Genetics Identifies Plant CPL Phosphatases as Regulators of miRNA Processing Factor HYL1. Convergent Multi-miRNA Targeting of ApoE Drives LRP1/LRP8-Dependent Melanoma Metastasis and Angiogenesis.
The slicing activity of miRNA-specific Argonautes is essential for the miRNA pathway in C. elegans. MiRNA from PDB. Call for Papers: MiRNAs and Thyroid Cancer. The Role of MicroRNA ‘Sponges’ MiRNA Blog - microRNA Research and Industry News. MiRNA microarray. Bioinformatics Answers on BioStar. Lack of miRNA Misregulation at Early Pathological Stages in Drosophila Neurodegenerative Disease Models. InteRNA Gets Nod for US Patent, Plans IND for miRNA Melanoma Drug in Late 2013 | Gene Silencing News | RNAi. InteRNA Technologies - miRNA therapeutics for cancer. Integrative analysis of gene and miRNA expression profiles with transcription factor–miRNA feed-forward loops identifies regulators in human cancers.
Transcriptome and Small RNA Deep Sequencing Reveals... [J Pathol. 2012. MiRNA Profiles of Monocyte-Lineage Cells Are Consistent with Complicated Roles in HIV-1 Restriction. Retraction: Comparative expression profile of miRNA... [PLoS One. 2012. Discovery of hundreds of mirtrons in mouse and human small RNA data. Treatment-independent miRNA signature in blood of wilms tumor patients. Bottlenecks Caused by Software Gaps in miRNA and R... [Pharm Res. 2012. Identification of soybean seed developmental stage-specific and tissue-specific miRNA targets by degradome sequencing. Converging miRNA functions in diverse brain disor... [Exp Neurol. 2012.