Primers and Sequencing
BioMath - Tm Calculations for Oligos There are a number of different ways to calculate the melting temperature (Tm) of an oligo. All of these methods will give different results. Please note that these calculations are theoretical. Optimum Tm values must be determined empirically. For details on each of the calculations performed below please see the notes below, "Theoretical Tm of Oligos".
Seamless Cloning & Genetic Assembly
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