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The importance of pH in HPLC. For some separations the effect of changing pH is minimal. However for acids and bases, the effect of even a very small change in pH is very significant. Changing the pH changes the degree of ionisation of molecules in solution. It thus affects their polarity, and as a consequence it changes their retention times in an HPLC separation.
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Introduction Choose the buffer species you want to use, and enter parameters for volume, pH, and concentration of buffer species.