Microbiologynote

Confocal Microscope Principle, Uses, Parts, Advantages, and Disadvantages. A traditional simple microscope creates a murky and fuzzy image of 3-dimensional objects, because light from all areas of the object, not just the plane of focus, enters the microscope.

This problem has been solved by the confocal scanning laser microscope (CSLM), or simply, confocal microscope. Confocal Microscope Confocal Microscope also called confocal laser scanning microscopy (CLSM) or laser confocal scanning microscopy (LCSM).A confocal microscope creates a high optical resolution and high contrast image with a spatial pinhole, which blocks out-of-focus light in image formation.In an ordinary simple microscope, light passes through the sample, whereas in a confocal microscope focuses a smaller beam of light at one narrow depth level at a time.It creates a 3D picture of the specimen by using a technique, called optical sectioning.

Oil Immersion technique, objectives, Resolving Power, Used for, Types. What is Oil Immersion?

Oil immersion is a technique, used to increase the resolving power or microscopic resolution of a light microscope.This is done by immersing the objective lens and specimen into a transparent oil containing a high refractive index, as a result, it increases the numerical aperture of the objective lens.The immersion oils have the index of refraction around 1.515.The objective lens, which is immersed in the immersion oil is called an oil immersion objective. What is Microscopic Resolution? You Might Like This Articles: Microscopic Resolution Definition The microscopic resolution also is known as resolving power.

Equation of Microscopic resolution The microscopic resolution can be calculated by using Abbe Equation. The Abbe equation is; Where,d = Distance between two objects.𝞴 = Wavelength of light.nsin𝝧 = Numerical Aperture.When d becomes smaller, the resolution increases, therefore the resolution (r ); Why is Oil Immersion used? Immersion oil Objectives Reference. Inverted microscope Images and definition, principle, Uses, Parts. Inverted microscope Definition Inverted microscopes are almost similar to a simple microscope, but all the components are placed in the inverted condition.

The Inverted microscope comes with its own light source and a condenser lens. Which are located at the top portion of the microscope and is pointing down. Whereas the objectives and the turret are located below the stage, pointing upwards. In this microscope, we observe the specimen from down, (upwards) instead of from the up. Inverted microscopes come with three to six objective lenses.

In 1850, a faculty member of Tulane University J. Dissecting Microscope (Stereo Microscope) Definition, Uses, Parts, Principle. Dissecting Microscope Definition Dissecting microscope also known as stereo or stereoscopic microscope.

It is an optical microscope that is designed for low magnification observation of a specimen.It uses the reflected light rays from the specimen surface instead of transmitted light rays.It provides low power magnification as compared to compound microscopes.The magnification power of a Dissecting microscope ranges from 5x-80x.It produces a three-dimensional image of the specimen rather than a flat image.Dissecting microscope contains two separate objective lens and eyepiece, which creates two separate optical paths for each eye.

Top 32 Difference Between Light Microscope and Electron Microscope. There are present different types of microscope and each microscope contains different features.

The most common microscopes are Light microscope and electron Microscope. These two microscopes contain distinct features for different purposes. Top 32 Difference Between Light Microscope and Electron Microscope. Top 32 Difference Between Light Microscope and Electron Microscope. Cell Structure and Functions. Cell Structure and Functions.

Cells are the tiny units of life that emerged on Earth at least 3.5 billion years ago. Every living thing is made up of cells, starting from the blue whales to the archaebacteria that live inside volcanoes. Our body contains approximately 40 trillion (4×1013) cells (the human brain contains around 80 billion cells). The cells are doing millions of things at once in our body such as electron transport, pumping blood, filtering urine, digesting food, making protein, storing fat, and that’s just the stuff you’re not thinking about! What is the cell? Prokaryotic Cell and Eukaryotic Cell. In the previous article we learned about cell structure and functions.

Now in this article we will learn about Prokaryotic and eukaryotic cells. Introduction Hey, if I told you, that you are surrounded by 100 trillion bacterial cells, would you believe? Yes, If you look closer than you will find trillions of bacterial cells on your skin surface or inside your digestive tract. All of these cells are living on you are classified into two major classes called prokaryotic and eukaryotic. Difference Between Prokaryotic and Eukaryotic Cells. Difference Between Prokaryotic and Eukaryotic Cells Follow previous articles: Reference.

Virus Structure and Application, Origin, Definition, Characters, and Example. To Listen This Aticle Just Click On this Play Button Introduction It is estimated that there is approximately 10^31 virus on earth, which is over ten million times more than the entire stars in the universe.

All of these viruses are not infectious to humans, most of them are living in oceans and they attack bacteria and other microbes. What is Virus? Virus is a microscopic, infectious particle that can reproduce or replicate within the living cell of an organism or host. In 1898, a Dutch scientist, Martinus Beijerinck discovered the world’s first virus which was the tobacco mosaic virus. Are viruses alive or dead? Viruses are neither living nor a dead cell. But also they are not alive because, at the outside of host cell they act as a nonliving cell.

HomeostasisThe first and important criteria of a living thing are – it must be made of cells.The virus particles don’t follow this criteria because they are not made of cells. Virus Structure and Application, Origin, Definition, Characters, and Example.