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TibidyHealth : #RNA switches can better equip... NewsMedical : RNA switches can better equip... TibidyHealth : #RNA switches can better equip... Cancer_bio : Alnylam and Collaborators Publish... Mdsdaily : PPR proteins recognise RNA... Mdsdaily : PPR proteins recognise RNA... TibidyHealth : #PPR proteins recognise #RNA... ScienceIndex_ : A novel... TibidyHealth : All about RNA-binding. Tagged... TibidyHealth : Describing For The First Time... ScienceIndex_ : Fcp1... Sciencemagazine : RNA-directed targeting system... Rna-delivering. ResearchBlogs : Interacting small RNA pathways... ScienceIndex_ : Efficient... ScienceDaily : Molecular code cracked: Code... MyScienceCareer : #Jobs Research on RNA molecular... Leclercfl : Western Wildfires... ScienceIndex_ : Global... TibidyHealth : New Method Introduced To Closely... TibidyHealth : RNA-delivering nanoparticles... TibidyHealth : All about RNA-delivering. Tagged... FurnoX : Single-Stranded siRNAs Activate... ScienceIndex_ : Analysis...
ScienceIndex_: ScienceDaily : The making and unmaking of... ScienceIndex_: Inhibition... Leclercfl : Kelvin K. Ogilvie Kelvin K. Science Magazine: Sign In. RazZ0r: Predicting in vivo binding... RNA-delivering nanoparticles allow rapid screening of new drug targets in mice. By sequencing cancer-cell genomes, scientists have discovered vast numbers of genes that are mutated, deleted or copied in cancer cells.
This treasure trove is a boon for researchers seeking new drug targets, but it is nearly impossible to test them all in a timely fashion. To help speed up the process, MIT researchers have developed RNA-delivering nanoparticles that allow for rapid screening of new drug targets in mice. In their first mouse study, done with researchers at Dana-Farber Cancer Institute and the Broad Institute, they showed that nanoparticles that target a protein known as ID4 can shrink ovarian tumors.
The nanoparticle system, described in the Aug. 15 online edition of Science Translational Medicine, could relieve a significant bottleneck in cancer-drug development, says Sangeeta Bhatia, the John and Dorothy Wilson Professor of Health Sciences and Technology and Electrical Engineering and Computer Science and a member of the David H. An abundance of targets. Life_Sciences_: Annotating... BMC_series : RT @rnomics: Analysis of... Science Magazine: Sign In.
Using single-molecule fluorescence resonance energy transfer, we have defined bacterial RNA polymerase (RNAP) clamp conformation at each step in transcription initiation and elongation. We find that the clamp predominantly is open in free RNAP and early intermediates in transcription initiation but closes upon formation of a catalytically competent transcription initiation More Using single-molecule fluorescence resonance energy transfer, we have defined bacterial RNA polymerase (RNAP) clamp conformation at each step in transcription initiation and elongation. We find that the clamp predominantly is open in free RNAP and early intermediates in transcription initiation but closes upon formation of a catalytically competent transcription initiation complex and remains closed during initial transcription and transcription elongation. We show that four RNAP inhibitors interfere with clamp opening. FurnoX: Viroid RNA redirects host DNA... BioMedCentral: sRNAdb: A small non-coding...
FurnoX: Site-specific DICER and DROSHA... View All. RazZ0r: RNA regulons: coordination... Viroid RNA redirects host DNA ligase 1 to act as an RNA ligase. María-Ángeles Nohales , Ricardo Flores , and José-Antonio Daròs 1 + Author Affiliations Edited by George E. Bruening, University of California, Davis, CA, and approved July 9, 2012 (received for review April 13, 2012) Abstract Viroids are a unique class of noncoding RNAs: composed of only a circular, single-stranded molecule of 246–401 nt, they manage to replicate, move, circumvent host defenses, and frequently induce disease in higher plants. RNA ligation RNA replication RNA processing Footnotes Author contributions: M. The authors declare no conflict of interest. This article is a PNAS Direct Submission. PPR proteins recognise RNA targets via easy-to-understand code. Published on August 20, 2012 at 12:41 PM The code determines the recognition of RNA molecules by a superfamily of RNA-binding proteins called pentatricopeptide repeat (PPR) proteins.
When a gene is switched on, it is copied into RNA. This RNA is then used to make proteins that are required by the organism for all of its vital functions. If a gene is defective, its RNA copy and the proteins made from this will also be defective. This forms the basis of many terrible genetic disorders in humans. RNA-binding PPR proteins could revolutionise the way we treat disease. The new paper in PLOS Genetics describes for the first time how PPR proteins recognise their RNA targets via an easy-to-understand code.
"Many PPR proteins are vitally important, but we don't know what they do. "What's more, we can now design our own synthetic proteins to target any RNA sequence we choose - this should allow us to control the expression of genes in new ways that just weren't available before. Molecular code cracked: Code determines recognition of RNA molecules. Scientists have cracked a molecular code that may open the way to destroying or correcting defective gene products, such as those that cause genetic disorders in humans. The code determines the recognition of RNA molecules by a super-family of RNA-binding proteins called pentatricopeptide repeat (PPR) proteins.
When a gene is switched on, it is copied into RNA. This RNA is then used to make proteins that are required by the organism for all of its vital functions. If a gene is defective, its RNA copy and the proteins made from this will also be defective. This forms the basis of many terrible genetic disorders in humans. RNA-binding PPR proteins could revolutionize the way we treat disease. The new paper in PLoS Genetics describes for the first time how PPR proteins recognize their RNA targets via an easy-to-understand code. "Many PPR proteins are vitally important, but we don't know what they do.
Interacting small RNA pathways in worms 5: Global Genome Surveillance | biobabel. As discussed previously, in C. elegans more than 15,000 21U-RNAs are expressed from two large clusters on chromosome IV. As very few of these piRNAs exhibit perfect sequence complementarity with other endogenous sequences within in the C. elegans genome, it’s been difficult to deduce the targets and functions of this system. New studies from the labs of Eric Miska and Craig Mello have significantly advanced our understandings of piRNAs in C. elegans. Bagijn et al. and Lee et al. have confirmed a previous supposition that 21U-RNAs can base-pair with imperfectly complementary sequences. This less stringent base-pairing opens up the entire transcriptome to possible piRNA-mediated regulation.
Targets Previous studies had shown that 21U-RNAs act upstream of 22G-RNAs to repress the activity of Tc3 transposons. 22G-RNAs are not evenly distributed on their target mRNAs. Bagijn et al performed slightly different analyses, which came to similar conclusions. PiRNA sensors Long-term heritability. Study identifies how RNA viruses hijack a host cell to multiply. Study identifies how RNA viruses hijack a host cell to multiply. (Phys.org) -- By discovering how certain viruses use their host cells to replicate, UC Irvine microbiologists have identified a new approach to the development of universal treatments for viral illnesses such as meningitis, encephalitis, hepatitis and possibly the common cold.
The UCI researchers, working with Dutch colleagues, found that certain RNA viruses hijack a key DNA repair activity of human cells to produce the genetic material necessary for them to multiply. For many years, scientists have known that viruses rely on functions provided by their host cells to increase their numbers, but the UCI study – led by microbiology & molecular genetics professor Bert Semler – is the first to identify how the RNA-containing picornaviruses utilize a DNA repair enzyme to do so. Study results appear in the early online edition of the Proceedings of the National Academy of Sciences the week of Aug. 20. Explore further: Microgravity research helping to understand the fungi within. RNA switches can better equip and regulate viruses for many therapeutic applications.
Tiny RNA fragments control bacterial infections | Lab Rat. There is more than one type of genetic material within the cell. As well as DNA, which stores the code for making cellular protiens, there is also RNA, which contains similar snatches of code but is less stable and more mobile than DNA. If DNA is a library of books which are not allowed to be removed, then RNA is little buts of paper containing copies of pages that are spread around for people to read. The differences between RNA (left) and DNA (right). RNA is also found as a double helix, a triple helix and a strange looped thing. Image from wikimedia commons, credit link below. Given its power to act as an intermediary between DNA and protiens, which are two of the most important macromolecules within the cell, RNA has a huge number of jobs to do. One of those jobs is to regulate which parts of the DNA are making proteins.
In the case of the bacteria Streptococcus pneumoniae, the small RNAs can turn on the parts of the DNA required to make the bacteria virulent. Credit link for image 1. Physorg_com : A step forward toward muscular... TibidyHealth : #Affymetrix announces... ResearchBlogs : Interacting small RNAs in worms... ScienceIndex_ : Small... TibidyHealth : #Affymetrix announces... Interacting small RNA pathways in worms 4: 21U-RNAs. Protein-rna. TibidyHealth : 'Antisense' Compound #Rids... Interacting small RNA pathways in worms 4: 21U-RNAs. Rna-seq. Sciencemagazine: RNA polymerase "clamp" opens... ScienceIndex_ : Kraken... Leclercfl : Central Dogma = Central Di... ScienceIndex_: Different... FurnoX: RT @Jen_Angela: siRNA-producing... TibidyHealth : First Complete #Atlas Of #... Sciencemagazine: RNA polymerase "clamp" opens... Medical_xpress : A step forward toward muscular...
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