Calculator for pH buffers. Hydrochloric acid - OpenWetWare. Hydrochloric acid, abbreviation HCl(aq), is a common acid both in the body and in the lab.
It is, for example, a major component of gastric acid (pH 1-2, 0.5% w/v HCl). In experiments, it is used among other things to set the pH in buffers (e.g. Tris) and to reveal antigens (e.g. BrdU). Chemically speaking, it is a solution of the gas hydrogen chloride = HCl(g) in water. Conversion % HCl to M HCl chemical/physical data 37% w/w HClaq has a density of 1.19 kg/L molecular weight of HCl = 36.5 g/mol  % to molar conversion % w/w HCl to w/v HCl: 37% HCl x 1.19 kg/L density = 0.44 kg/L HClw/v to mol/v: 0.44 kg/L / 36.5 g/mol = 12 mol/L. Lab Math: A Handbook of Measurements, Calculations, and Other Quantitative Skills for Use at the Bench, 2nd edition. Description Lab Math: A Handbook of Measurements, Calculations, and Other Quantitative Skills for Use at the Bench, 2nd edition, collects in one place the numbers and equations you rely on for your experiments and use to report your data—what they mean and how to use them—as well as easy-to-follow shortcuts for making the math easier.
Acid guanidinium thiocyanate-phenol-chloroform extraction. Acid guanidinium thiocyanate-phenol-chloroform extraction (abbreviated AGPC) is a liquid–liquid extraction technique in biochemistry.
It is widely used in molecular biology for isolating RNA (as well as DNA and protein in some cases). This method may take longer than a column-based system such as the silica-based purification, but has higher purity and the advantage of high recovery of RNA: an RNA column is typically unsuitable for purification of short (<200 nucleotides) RNA species, such as siRNA, miRNA, gRNA and tRNA. It was originally devised by Piotr Chomczynski and Nicoletta Sacchi, who published their protocol in 1987. The reagent is sold by Sigma-Aldrich by the name TRI Reagent; by Invitrogen under the name TRIzol; by Bioline as Trisure; and by Tel-Test as STAT-60. How it works Guanidinium thiocyanate denatures proteins, including RNases, and separates rRNA from ribosomal proteins, while phenol, isopropanol and water are solvents with poor solubility.
Reagents Molecular cloning: A laboratory manual. Second edition. Volumes 1, 2, and 3. Current protocols in molecular biology. Volumes 1 and 2: Cell. Laemmli Sample Buffer (4×) Laemmli Buffer: What Is It for Anyway? - Bitesize Bio. Electrophoresis encompasses a wide range of techniques in which charged biomolecules in a liquid, a solid, or a semisolid solution can be separated by size under the application of an electric field.
The most common application of electrophoresis for the separation of proteins is SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis), which has been previously covered here. The most important step of electrophoresis is actually the extraction of proteins from a sample. During this process certain conditions need to be met in order to get an optimal result. Although protein extraction methods can vary, Laemmli buffer is a constant in nearly all protocols.
Taking its name from Prof. Seven tips to manage your mental health and well-being during the COVID-19 outbreak. I am a clinical psychologist who specializes in academic mental health and well-being.
In mid-March I was due to run a resilience and well-being workshop in Sweden for doctoral candidates, but was instead facing day eight of quarantine with two small children in my apartment in Spain. Like many people’s, my sense of resilience feels increasingly frayed. When our minds are consumed by the spread of the coronavirus and its impact on our health, loved ones, home countries, economy and students — not to mention our research programme, funding or employment status, and an abrupt transition to e-learning — how do we maintain our own mental health and well-being and that of our community? Here are some tips that have resonated from discussions I have held with academic leaders and students about responding to COVID-19: Manage your expectations. How I learned to teach like a scientist. After a fulfilling career as a college biology professor, I’m retiring.
“What will you miss most?” A colleague asked. My answer was something that, 30 years ago, I would never have expected myself to say: “I will miss the creativity of teaching.” When I was a new faculty member, I considered teaching a necessary evil that took me away from the lab bench. I wanted to focus on research, guiding graduate students in what I hoped would be groundbreaking studies on nerve growth. Last week’s Working Life Working Life is a personal essay series about career issues, challenges, and successes. I was 10 years into my career, happily plugging away at my research as a tenured professor, when my teenage niece was orphaned and I became her guardian and single parent. It was hard to drop a research program that—up to that point—had defined my career and fueled my passions. A clue to the problem came when I took a look at the introductory biology textbooks they had studied in earlier classes. LabAgenda. (1003) 1_How To Make 2 M HCl & 2 M NaOH Solutions. Preparation of 10 M Sodium hydroxide (NaOH) solution - Lab Protocols.
A 10N sodium hydroxide stock solution is prepared which is used for many applications including including adjusting pH of various solutions.
ReagentsNaOH palletDeionized / Double distilled waterEquipment and disposablesMeasuring cylinderConical flask / BeakerMagnetic stirrer Composition: 10 M NaOH solution Objective: Preparation of 100 ml of 10 M NaOH solution in water Step 1: To prepare 100 ml of 10 M NaOH solution, weigh out 40 g of NaOH (Mol. Wt. 40). Precaution: Don’t add water in the NaOH pellet.