MagHelix™ Saturation Transfer Difference (STD) NMR - Creative Biostructure. Various nuclear magnetic resonance (NMR) spectroscopy applications have been developed for the identification and validation of hit compounds and lead optimization.
NMR has many advantages, such as the ability to directly observe target proteins and compounds and can be used for protein-based and ligand-based approaches. NMR spectroscopy can also offer significant information about the interactions in the protein-ligand complex, such as structure, affinity, and kinetics, especially in cases where the interactions are too weak to be detected by other approaches or where high-quality crystals of protein-ligand complex cannot be obtained. MagHelix™ Dynamic Light Scattering (DLS) - Creative Biostructure. Assessing the developability of drug candidates in the early stage of drug development is important to reduce the risk of costly downstream failure.
Dynamic light scattering (DLS), also known as photon correlation spectroscopy, has become one of the main techniques for evaluating the aggregation, stability, and viscosity of therapeutic proteins, monoclonal antibodies, peptide drugs, and other biopharmaceuticals due to its ability to measure the size distribution of macromolecules and nanoparticles. Moreover, this technique can also be utilized to identify promiscuous inhibitors from hit compounds screened from small molecule compound libraries. MagHelix™ Quartz Crystal Microbalance-Dissipation (QCM-D) - Creative Biostructure. In the early stage of drug development, the process of screening and characterizing compounds that interact with cellular and protein targets is essential.
Creative Biostructure provides MagHelix™ Quartz Crystal Microbalance-Dissipation (QCM-D) solutions for drug discovery departments in the pharmaceutical industry and biotechnology companies to detect the activity of compounds on specific biological macromolecular targets or whole cells. QCM-D technology ensures that the detection process is carried out in a high speed, small sample quantities, real-time and economical way.
Our QCM-D services detect the kinetics and magnitude of a cell, membrane, and protein drug targets response with respect to ligand binding, providing a wealth of information for effective drug screening. Brief Introduction to Quartz Crystal Microbalance-Dissipation (QCM-D) Figure 1. MagHelix™ Isothermal Titration Calorimetry (ITC) - Creative Biostructure. In the early stage of drug discovery, the kinetic and thermodynamic characterization of ligands that bind to specific target proteins can provide vital information for the identification and optimization of hit or lead compounds.
The thermodynamics and kinetics of the protein-ligand association provide mechanistic insights into molecular interactions that determine the affinity of ligands to their specific target proteins. More detailed information besides the significant binding affinity can be obtained from thermodynamic characterization, such as the driving force for the ligand to associate with its target.
The combination of protein-ligand structure information and thermodynamic parameters can help to fully understand the structure-activity relationship (SAR). Moreover, the kinetic parameters of protein-ligand recognition also reveal changes in binding events over time, which may be closely related to drug efficacy. MagHelix™ Surface Plasmon Resonance (SPR) - Creative Biostructure.
Optical biosensors have been utilized widely in many links and areas of drug discovery, such as target identification, ligand screening, lead compound selection, early ADME as well as manufacturing quality control.
Among them, surface plasmon resonance (SPR) technology is a rapidly developing approach for determining binding events, which can characterize target protein-ligand interactions in real time. Creative Biostructure has extensive expertise and experience in SPR technology, and we can exploit this technology to provide sensitive, rapid, and reliable assays to help you measure real-time binding and kinetic information of ligands against specific targets. Our MagHelix™ SPR is a customizable solution that optimizes procedure for specific drug discovery projects, from experimental design to downstream applications. Brief Introduction to Surface Plasmon Resonance (SPR) MagHelix™ Co-crystallization and Soaking - Creative Biostructure. Rapid access to detailed structural information about drug targets and bound ligands is significant in the identification and optimization of lead compounds.
The structural information of the protein-ligand complex can accelerate the optimization process of potential lead compounds and help to solve problems related to compound selectivity, pharmacokinetics, as well as patentability. X-ray crystallography has always been a major technology to provide high-resolution protein-ligand complex structures for the pharmaceutical industry. As a professional contract service provider in the field of structural biology, Creative Biostructure offers high-throughput crystallography techniques for lead compound identification and optimization to assist you to obtain a large number of target-ligand (such as fragments, compounds) complex structures in a short time.
The crystal structure is useful for understanding the binding mode of ligands and thus help guide the optimization of the lead compound. MagHelix™ Toxicity Assays Using Zebrafish - Creative Biostructure. Creative Biostructure is a biotechnology company offering drug discovery contract services to pharma, biotech, chemical companies, and academic institutes.
Based on our zebrafish screening platform, we can provide MagHelix™ toxicity assays using zebrafish. Zebrafish has been a prominent model vertebrate for investigating chemical toxicity. We aim at developing new and faster screen/bioassays using zebrafish as an animal model, as well as new technologies for optimizing the maintenance and breeding of zebrafish. Figure 1. Visualization of the zebrafish embryo vasculature by gfp:fli1 expressing endothelial cells. MagHelix™ Zebrafish Disease Model Generation - Creative Biostructure. Zebrafish has been widely used as an ideal model to find genes involved in specific processes and/or check the activity of a desired gene in a specific process.
Over the last decade, it has also been developed into a valuable system for the study and modeling of various human diseases, as well as the discovery of relevant drug candidates. The development and functionality of zebrafish organs are essentially similar to those of humans; and the ease of genetic modifications has further facilitated the generation of disease models in the zebrafish system. Fish and humans share high functional similarities in a group of genes involved in physiological processes.
The zebrafish model plays an important role in the understanding of human disease pathogenesis and ultimately the discovery of new therapeutic agents for a wide range of human diseases. Figure 1. Cryo-EM for Subcellular Organelles. Cryo-EM for Subcellular Organelles Cryo-Electron Microscopy (Cryo-EM) can present samples in a native state with imaging the sample’s intrinsic electron density, providing direct visualization of biological macromolecules, such as protein, nucleic acid, large complexes and so on.
Conversely, X-ray crystallography must require crystallization of the specimen within non-physiological environments. The resolution of Cryo-EM maps has been improved up to near-atomic resolution level. Thus Cryo-EM is gaining popularity in structural biology. Cryo-Electron Tomography (Cryo-ET), one of the techniques of Cryo-EM, is used to produce three-dimensional (3D) pictures from a series of tilted Cryo-EM images. At Creative Biostructure, we have the most advanced Electron Microscopes equipments and software. The advantages of our service include: Solid-state NMR Services. X-ray crystallography, nuclear magnetic resonance (NMR) and Cryo-electron microscopy (Cryo-EM) are three of the most predominant techniques in structure biology.
They are powerful tools that help structural biologists get an insight into the bioprocess mechanism. NMR is a non-destructive method which providess information on chemical structure and dynamics with atomic resolution. Unlike solution-state NMR, which is good at resolving small protein structure and obtaining dynamic information of soluble materials, solid-state NMR (ssNMR) is a powerful technique for the characterization of solid materials. With solid-state NMR methods, information about structure symmetry, dynamics, and interactions of molecules and complexes can be obtained. Discovery Program for COVID-19 Targets. Discovery Program for COVID-19 Targets The 2019 novel coronavirus (provisionally named 2019-nCoV) is a contagious virus that causes respiratory infection.
From genomic sequencing, it has been shown as a positive-sense, single-stranded RNA coronavirus. The virus was first detected in Wuhan, China, in mid-December 2019 and subsequently spreads to all provinces of China and to more than twenty other countries over the world. Creative Biostructure has established an advanced COVID-19 targeted discovery platform to offer a wide variety of products and services to accelerate your research for structure/function study and novel drug discovery. Our featured services and products include: Exosome Lipidomics & Metabolomics. Exosome Lipidomics & Metabolomics Creative Biostructure has long been focused on the research related to disease.
We offer comprehensive analytical services for exosome lipidomics and metabolomics, which support you to find potential biomarkers much easier. The Importance of Exosome Lipidomics & Metabolomics Analysis Exosomes contain various compositions, including proteins, nucleic acids, lipids, and other metabolites, which facilitating cell-to-cell communication. In vivo cells, lipids usually act as medium for signal transduction. Exosome Lipidomics & Metabolomics service. Exosome Analysis and Characterization. Exosome Analysis and Characterization Creative Biostructure's exosome analysis and characterization service makes it easy to profile exosomal lipids, proteins and RNAs with our years of experience working on exosomes.
Our expertise provides comprehensive support for your exosome engineering study by including the following customized services. The Importance of Exosome Analysis and Characterization Characterization of exosomes has become a topic of interest in medical research and molecular biology because it can provide very valuable information for early detection, disease monitoring and development of effective treatments against cancer and autoimmune diseases. Therefore, to acquire a deeper knowledge of the exosome biology and accelerate the use of these extracellular vesicles in diagnostic tests or clinical research, Creative Biostructure provides a sensitive, reproducible methods for exosome characterization. Figure 1. Exosomal Lipidomics & Metabolomics Services. Cyclodextrin-Based Liposomes Production. Cyclodextrin-Based Liposomes Production Using dual approach has emerged as a novel plan to tackle the problems of poor aqueous solubility and low rate of dissolution in drug formulation.
Therefore, Creative Biostructure combines the cyclodextrins and nanotechnology to develop cyclodextrin-based liposomes that deliver hydrophobic drugs. How Cyclodextrin-Based Liposomes Work Cyclodextrins (CDs) are cyclic oligosaccharides formed from glucopyranose units. With hydrophobic cavity inside and hydrophilic outside, they can form complexes with hydrophobic compounds. By complexing with the cyclodextrin and their derivatives, the solubility, stability, and bioavailability of drug can be improved, along with reduced toxicity and modified physicochemical characteristics. Cryo-EM for Bacteriophages. Cryo-EM for Bacteriophages It is crucial that understanding the biological functions of macromolecules by characterizing the molecular structure. As the most effective method to obtain atomic models of proteins and nucleic acids in the 20th century, X-ray crystallography has been fundamental in the development of many scientific fields. But many samples have been proved that it is difficult to crystallize (for example membrane proteins).
In addition, the biological macromolecular complexes sometimes cannot be produced in sufficient quantities of crystallization. COVID-19 Related VLP Products. MagHelix™ Surface Plasmon Resonance (SPR) - Creative Biostructure. MagHelix™ Saturation Transfer Difference (STD) NMR - Creative Biostructure. MagHelix™ Microscale Thermophoresis (MST) - Creative Biostructure. Microscale thermophoresis (MST) has become a common technique to detect specific target-probe interactions, and it measures the differences in the movement rate through a microscopic temperature gradient caused when complexes are formed. MST can be applied to characterize any type of biomolecular interactions, for example, protein-DNA, protein-RNA, protein-protein, antigen-antibody interactions, as well as the binding of a ligand to ternary complexes.
MagHelix™ Differential Scanning Calorimetry (DSC) - Creative Biostructure. In addition to isothermal titration calorimetry (ITC), another microcalorimetry technique utilized to study protein-ligand interactions is differential scanning calorimetry (DSC). ITC measures the heat generated or consumed after titration of a ligand onto a target protein (or the reverse). The data obtained from an ITC experiment include Kd, stoichiometry, change in entropy (ΔS), change in enthalpy (ΔH), and the heat capacity change (ΔCp). DSC can measure the thermal changes and corresponding thermodynamics due to the thermal unfolding of a protein. MagHelix™ Bio-layer Interferometry (BLI) - Creative Biostructure. MagHelix™ Co-crystallization and Soaking - Creative Biostructure. MagHelix™ Efficacy Testing Using Zebrafish - Creative Biostructure. Current requirements for drug release not only require in vitro but also in vivo assays to increase drug efficacy and minimize toxicity.
Characterizing the efficacy and/or safety of compounds in the preclinical phase can effectively reduce the cost of the clinical phase. The zebrafish (Danio Rerio) is one of the most utilized vertebrate models in recent years. In terms of molecular genetics, zebrafish and human have high genetic homology. Moreover, the zebrafish has mammalian-like organs. Zebrafish embryos are transparent allowing directly assess the in vivo effects of drugs on organs and tissues. Creative Biostructure is a leading biotechnology company committed to streamlining discovery and preclinical development of novel compounds with the zebrafish system. MagHelix™ Toxicity Assays Using Zebrafish - Creative Biostructure. Liposomes Technology. Creative Biostructure has been focused on the development of liposome technologies for years, and our rich experience makes us an expert in liposome preparation and manufacuring.
The ultimate goal of our Liposomes Platform is to provide you the best liposomes services all the way from custom liposome production, analysis and characterization to the application. Figure 1. Custom MemPro™ T-cell Surface Glycoprotein CD3 Zeta Chain. Home. Plasmids Design and Construction. Discovery Programs for Ion Channel Targets. Creative Biostructure has established a magic ion channel targeted discovery platform to provide comprehensive products and services to accelerate your research for novel drug discovery and safety pharmacology. Ion channel is pore-forming membrane protein that facilitate ions to pass through the plasma membrane, playing crucial roles in the physiology of all kinds of cells. Functions of Ion Channels Ion Channel Classification Ion channel have been identified into various types by gating mechanism or ion selectivity. Figure 1.
Combinatorial Assembly Libraries. Creative Biostructure has rich professional experience in custom combinatorial assembly libraries services for directed protein evolution. The combinatorial assembly library offers a great platform for protein function and active center studies. Combinatorial assembly library is a method to build customized variants of DNA parts (e.g. promoters, ribosomal binding sites, UTRs, open reading frames, signal peptides terminators, etc.) that combine multiple genetic elements in predefined arrangements. Mempro™ Detergent-Free Membrane Anchor Helices of Peripheral Proteins Production. Liposomes Technology. Custom Affinity Chromatography Service. De Novo Drug Design - Creative Biostructure.
Molecular Docking - Creative Biostructure. High-content Screening (HCS) - Creative Biostructure. Ligand-based Virtual Screening - Creative Biostructure. In Silico Virtual Screening - Creative Biostructure. High-throughput Screening (HTS) - Creative Biostructure. MagHelix™ HTS Assay Development - Creative Biostructure. MagHelix™ Library Design and Preparation - Creative Biostructure. Solid-state NMR Services. Cyclodextrin-Based Liposomes Production. Cryo-EM for Subcellular Organelles. Discovery Program for COVID-19 Targets. Exosome Lipidomics & Metabolomics Services. Cryo-Electron Tomography. Exosome Lipidomics & Metabolomics Services. Exosome Isolation and Purification. Cryo-EM for Filaments. Discovery Program for COVID-19 Targets. Exosome Lipidomics & Metabolomics Services. Exosome Analysis and Characterization. Cyclodextrin-Based Liposomes Production. Liposomes Technology. Cryo-EM for Subcellular Organelles. Cryo-Electron Tomography.
Cryo-EM for DNA Samples. Mempro™ Virus-like Particles (VLPs) Functionalization Strategies. MagHelix™ Differential Scanning Calorimetry (DSC) Lipidic Cubic Phase Crystallization. Data Processing and Interpretation. Bicelle-Protein Crystallization. Data Processing and Interpretation. EM Data Processing and Interpretation. NMR Data Processing and Interpretation. Batch Screening for Crystals Production. Mempro™ Nanodisc Technology. Mempro™ Liposome Preparation. Mempro™ Virus-like Particles (VLPs) Characterization. Liposome Production. Optimization Screening. Trace Fluorescent Labeling Crystallization. Peptide Crystallization. Isotope Labeled Protein Production. Dynamics and Disorder Analysis. Quantitative Analysis.