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Genomics

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FloragenexRAD sequencing, Genotyping by Sequencing, SNP Discovery. Biota Sciences. GBS Overview. Genotyping by sequencing (GBS) is a simple highly-multiplexed system for constructing reduced representation libraries for the Illumina next-generation sequencing platform developed in the Buckler lab by Rob Elshire.

GBS Overview

Key components of this system are: reduced sample handling, fewer PCR and purification steps, no size fractionation and inexpensive barcoding. We use restriction enzymes to reduce genome complexity and avoid the repetitive fraction of the genome. MinION - $900 usb-powered DNA sequencer on sale this year. Oxford Nanopore (ON) has been developing a disruptive nanopore-based technology for sequencing DNA, RNA, proteins, and other long-chain molecules since its birth in 2005.

MinION - $900 usb-powered DNA sequencer on sale this year

The company has just announced that within the next 6-9 months it will bring to market a fast, portable, and disposable protein sequencer that will democratize sequencing by eliminating large capital costs associated with equipment required to enter the field. View all The MinION is the size of a USB memory stick, and obtains both power and computer analysis from a normal laptop computer. No polymerase chain reaction (PCR) or other DNA amplification technique is needed for optimum sensitivity.

Human Genome Project Information. Oxford Nanopore Technologies.