JOURNAL OF INTEGRATIVE AGRICULTURE - JULY 2021 - Biological and molecular characterization of tomato brown rugose fruit virus and development of quadruplex RT-PCR detection. Tomato brown rugose fruit virus (ToBRFV) is a novel tobamovirus firstly reported in 2015 and poses a severe threat to the tomato industry. So far, it has spread to 10 countries in America, Asia, and Europe. In 2019, ToBRFV was identified in Shandong Province (ToBRFV-SD), China. In this study, it was shown that ToBRFV-SD induced mild to severe mosaic and blistering on leaves, necrosis on sepals and pedicles, and deformation, yellow spots, and brown rugose necrotic lesions on fruits.
ToBRFV-SD induced distinct symptoms on plants of tomato, Capsicum annumm, and Nicotiana benthamiana, and caused latent infection on plants of Solanum tuberosum, Solanum melongena, and N. tabacum cv. Zhongyan 102. All the 50 tomato cultivars tested were highly sensitive to ToBRFV-SD. The complete genomic sequence of ToBRFV-SD shared the highest nucleotide and amino acid identities with isolate IL from Israel. JOURNAL OF PLANT DISEASES AND PROTECTION 05/07/21 Detection of Tomato brown rugose fruit virus (ToBRFV) in solanaceous plants in Mexico. Journal of Integrative Agriculture Volume 20, Issue 8, August 2021, Robust molecular detection of the new Tomato brown rugose fruit virus in infected tomato and pepper plants from Turkey. Abstract Tomato brown rugose fruit virus (ToBRFV) causes severe fruit loss in tomato (Solanum lycopersicum) and pepper (Capsicum annuum) plants. It is an emerging Tobamovirus that is spreading globally. The major challenge is to develop a reliable method for the detection of the virus, and to better characterize the symptoms it causes.
The aims of this study, therefore, were to characterize the symptom development on tomato and pepper plants, and to establish a reliable detection method for the virus. >Keywords tomato pepper Tobamovirus ToBRFV resistance. VIRUSES 25/08/21 New Resources for the Specific and Sensitive Detection of the Emerging Tomato Brown Rugose Fruit Virus. Plant viruses can evolve towards new pathogenic entities that may eventually cause outbreaks and become epidemics or even pandemics. Seven years ago, tomato brown rugose fruit virus (ToBRFV) emerged, overcoming the genetic resistance that had been employed for more than sixty years against tobamoviruses in tomato. Since then, ToBRFV has spread worldwide, producing significant losses in tomato crops. While new resistances are deployed, the only means of control is the implementation of effective prevention and eradication strategies.
For this purpose, in this work, we have designed, assessed, and compared an array of tests for the specific and sensitive detection of the ToBRFV in leaf samples. ►▼ Show Figures Figure 1 This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. PLANTS 21/06/21 Differential Detection of the Tobamoviruses Tomato Mosaic Virus (ToMV) and Tomato Brown Rugose Fruit Virus (ToBRFV) Using CRISPR-Cas12a. All articles published by MDPI are made immediately available worldwide under an open access license. No special permission is required to reuse all or part of the article published by MDPI, including figures and tables. For articles published under an open access Creative Common CC BY license, any part of the article may be reused without permission provided that the original article is clearly cited.
Feature Papers represent the most advanced research with significant potential for high impact in the field. Feature Papers are submitted upon individual invitation or recommendation by the scientific editors and undergo peer review prior to publication. The Feature Paper can be either an original research article, a substantial novel research study that often involves several techniques or approaches, or a comprehensive review paper with concise and precise updates on the latest progress in the field that systematically reviews the most exciting advances in scientific literature. Agronomía Mesoamericana - 2020 - Métodos de identificación del virus de la fruta rugosa marrón del tomate (ToBRFV) en México.
EspañolIntroducción. El virus de la fruta rugosa marrón del tomate (ToBRFV) se reportó por primera vez en Israel y Jordania en 2014, causa daños en cultivos de jitomate en agricultura protegida. Objetivo. Recopilar información de la distribución conocida, situación actual y métodos de detección del ToBRFV. Desarrollo. Entre 2018 y 2019 se confirmó la presencia del virus en México, Estados Unidos, China, Alemania, Turquía, Reino Unido e Italia. MAPAQ VIA YOUTUBE 15/01/21 Bilan des recherches récentes et en cours sur le virus du fruit rugueux brun de la tomate. RESEAU D'AVERTISSEMENTS PHYTOSANITAIRES (CA) 09/11/20 VIRUS DU FRUIT RUGUEUX BRUN DE LA TOMATE (ToBRFV) AFSCA 26/02/21 Mesures d’urgence contre le virus du fruit rugueux brun de la tomate. DG SANCO - Rapport OAV Greece 2020-7070 Greece Tomato brown rugose fruit virus.
VIRUSES 25/08/21 New Resources for the Specific and Sensitive Detection of the Emerging Tomato Brown Rugose Fruit Virus. AGRONOMY 22/06/21 Spatiotemporal Analyses of Tomato Brown Rugose Fruit Virus in Commercial Tomato Greenhouses. Tomato brown rugose fruit virus (ToBRFV) is an emerging pathogen affecting tomato-production systems in several countries, including Mexico. This situation involves challenges due to the negative impact on yield and the lack of disease-management measures.
This work analyzes the spatiotemporal distribution of ToBRFV in commercial tomato greenhouses. The presence or absence of diseased plants was evaluated weekly, assigning a location in space (x, y). Temporal analysis consisted of fitting the incidence to the monomolecular, logistic, log-logistic, Gompertz, exponential, Weibull, and Richard models, evaluated using the Akaike information criterion, significance, correlation, coefficient of determination, and root mean square error. ►▼ Show Figures Figure 1 This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited ►▼ Show Figures Figure 1.
VIROLOGY JOURNAL 06/01/21 Effectiveness of disinfectants against the spread of tobamoviruses: Tomato brown rugose fruit virus and Cucumber green mottle mosaic virus. 1.Dombrovsky A, Tran-Nguyen LTT, Jones RAC. Cucumber green mottle mosaic virus: rapidly increasing global distribution, etiology, epidemiology, and management. Annu Rev Phytopathol. 2017;55:231–56.CAS PubMed Article Google Scholar 2.Scholthof KB, Adkins S, Czosnek H, Palukaitis P, Jacquot E, Hohn T, Hohn B, Saunders K, Candresse T, Ahlquist P, Hemenway C, Foster GD. Top 10 plant viruses in molecular plant pathology. Mol Plant Pathol. 2011;12:938–54.CAS PubMed PubMed Central Article Google Scholar 3.Pagan I, Firth C, Holmes EC. Phylogenetic analysis reveals rapid evolutionary dynamics in the plant RNA virus genus tobamovirus.
GREENHOUSE CANADA 18/05/21 The race for rugose resistance - Researchers are revisiting ancestral tomato varieties in search of genetic tools against ToBRFV. As the world continues their battle with COVID-19, vegetable researchers and seed providers find themselves racing to develop resistance against a different pathogen. First identified in Israel in 2014, the tomato brown rugose fruit virus (ToBRFV, a.k.a. the rugose) is one of the most recent members of the tobamovirus genus to make headlines. Over the past several years, the virus has been reported in Mexico and the United States, as well as multiple countries in the EU including Spain, Italy and the Netherlands. According to the EPPO Global Database, Belgium reported its first case earlier this year in 2021, and China in 2019. The tobamovirus genus contains 35 known species, including the tobacco mosaic virus (TMV) and tomato mosaic virus (ToMV).
“This Tm-22 gene was the gold standard against tobamoviruses in tomatoes for the last 40 years, and it worked very well until ToBRFV emerged,” says Dr. Back to square one “Tolerance is good. “But they also found a resistance allele,” he adds. HORTI DAILY 17/05/21 Searching for the (real) cause of a ToBRFV infection. The Tomato Brown Rugose Fruit Virus (ToBRFV) originated in 2014 in the Middle East but is now a threat to all growers, wherever they are. The virus makes no distinction between conventional and organic growers. Once infected, both growers have to completely disinfect their greenhouse, keep it clean and do everything they can in order to prevent the virus from returning. However, not everyone succeeds in this, given the recurring infections at various growers. The outbreak of ToBRFV prompted organic crop consultants Aldo van Os and Mark Velders of The Conscious Farmer (TCF) to develop a practical preventive protocol but also to seek an answer to the question of why the virus is able to spread so rapidly and on such a large scale.
Seeking experienceTogether, they looked for practical experiences as soon as the virus emerged. After searching for some time, the TCF advisers finally found an 'expert by experience'. The very practical document has only 15 pages. For more information: FRESH PLAZA 11/05/21 Code for ToBRFV-resistant tomatoes cracked in Israel. TomaTech announces a major advancement in the fight against the Tomato Brown Rugose Fruit Virus (ToBRFV), which has caused significant economic damage estimated as much as billions of USD in the last few years globally. Led by Assaf Eybishitz Ph.D., the company furthered the fight against this pandemic of the tomato world by filing for a provisional patent for identifying the DNA markers correlated to its resistant traits which will accelerate the breeding and commercialization of high resistance non-GMO tomato varieties.
“Following years of intensive R&D efforts, TomatTech has succeeded in identifying DNA markers that elicit resistance to ToBRFV,” says Ofer Ben Zvi, CEO of TomaTech. “This discovery will allow us to rapidly develop a complete portfolio of resistant tomato varieties for global introduction and will benefit the growers and the entire value chain, providing a consistent supply chain while lowering production costs.” DG SANCO - Rapport OAV : Spain 2020-7104 Tomato brown rugose fruit virus. DG Health and Food Safety develops and publishes an annual work programme. Work priorities are identified following a careful process, based on legal obligations, risk posted in the sectors concerned and the Commission´s political priorities and in particular the Farm to Fork Strategy.
As there may be changes to the programme due to availability of resources, changes in risk or emerging issues, an update of the programme is usually presented in June. Abstract: This document sets out the work programme of the European Commission's Directorate for Health and food audits and analysis for 2021. It contains the details of controls planned in 2021 in the areas of food and feed safety, food quality, animal health, animal welfare, plant health and some areas of human health. FRONTIERS IN MICROBIOLOGY 21/04/21 Rapid and Sensitive Detection of Tomato Brown Rugose Fruit Virus in Tomato and Pepper Seeds by Reverse Transcription Loop-Mediated Isothermal Amplification Assays (Real Time and Visual) and Comparison With RT-PCR End-Poi.
Introduction Tomato brown rugose fruit virus (ToBRFV), a member of the genus Tobamovirus, family Virgaviridae, is an emerging and highly virulent virus, mainly affecting tomato crops worldwide. ToBRFV presents a genome organization common to tobamoviruses, with a single-strand positive-sense RNA of ~6,400 nucleotides (nt) with four open reading frames (ORFs) that encode two replication-related proteins (Salem et al., 2016). Since the first report of ToBRFV outbreak in tomato in Jordan (Salem et al., 2016), the virus has been identified within recent years in other countries on different continents: Israel (Luria et al., 2017), Mexico (Cambrón-Crisantos et al., 2018), United States (Chitambar, 2018; Ling et al., 2019), Germany (Menzel et al., 2019), Italy (Panno et al., 2019), Palestine (Alkowni et al., 2019), Turkey (Fidan, 2020), China (Yan et al., 2019), the United Kingdom (Skelton et al., 2019) and, more recently, in Greece (Beris et al., 2020).
Materials and Methods Table 1. Results. BIORXIV 17/03/21 Differential detection of Tomato mosaic virus (ToMV) and Tomato brown rugose fruit virus (ToBRFV) using CRISPR-Cas12. PLANTS 20/11/20 Tomato Brown Rugose Fruit Virus: Seed Transmission Rate and Efficacy of Different Seed Disinfection Treatments. Tomato brown rugose fruit virus (ToBRFV) is a highly infectious virus, that is becoming a threat to tomato production worldwide. In this work we evaluated the localization of ToBRFV particles in tomato seeds, its seed transmission rate and efficacy of disinfection, and the effects of different thermal- and chemical-based treatments on ToBRFV-infected seeds’ germination. Analyses demonstrated that ToBRFV was located in the seed coat, sometime in the endosperm, but never in the embryo; its transmission from infected seeds to plantlets occurs by micro-lesions during the germination.
The ToBRFV seed transmission rate was 2.8% in cotyledons and 1.8% in the third true leaf. Regarding the different disinfection treatments, they returned 100% of germination at 14 days post-treatment (dpt), except for the treatment with 2% hydrochloric acid +1.5% sodium hypochlorite for 24 h, for which no seed germinated after 14 dpt. PLANTS 19/01/21 Identification and Mapping of Tomato Genome Loci Controlling Tolerance and Resistance to Tomato Brown Rugose Fruit Virus. Tomato brown rugose fruit virus (ToBRFV) was identified in Israel during October 2014 in tomato plants (Solanum lycopersicum). These plants, carrying the durable resistance gene against tomato mosaic virus, Tm-22, displayed severe disease symptoms and losses to fruit yield and quality.
These plants were found infected with a tobamovirus similar to that discovered earlier in Jordan. This study was designed to screen and identify tomato genotypes resistant or tolerant to ToBRFV. The identified resistance and tolerance traits were further characterized virologically and genetically. ►▼ Show Figures Figure 1 This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
HORTI DAILY 05/03/21 Half of Sicilian companies report Tomato Brown Rugose Fruit Virus infections. PARLEMENT EUROPEEN - Réponse à question E-006117/2020 The brown wrinkle virus (ToBRFV) in tomatoes and peppers. MAPAQ VIA YOUTUBE 15/01/21 Que sait-on actuellement sur le virus de la rugose? UNIVERSITY OF FLORIDA - 2020 - Tomato Brown Rugose Fruit Virus (ToBRFV): A Potential Threat for Tomato Production in Florida. WAGENINGEN UNIVERSITY 23/10/20 New testing method available for Tomato brown rugose fruit virus (ToBRFV) USDA 13/05/20 USDA Taking Action to Protect the United States from Tomato Brown Rugose Fruit Virus. EUPHRESCO - 2020 - Validation of diagnostic tests for the detection and identification of Tomato brown rugose fruit virus in tomato and pepper seeds. AGRONOMY 12/06/20 Spread of Tomato Brown Rugose Fruit Virus in Sicily and Evaluation of the Spatiotemporal Dispersion in Experimental Conditions.
GROENTENNIEUWS_NL 11/05/20 TRAD AUTO : Cinq façons de laver les caisses, en éliminant efficacement ToBRFV cinq fois. ANSES - JANV 2020 - Évaluation du risque simplifiée du tomato brown rugose fruit virus pour la France métropolitaine.
ToBRFV au Royaume-Uni. EUPHRESCO - 2020 - Validation of diagnostic tests for the detection and identification of Tomato brown rugose fruit virus in tomato and pepper seeds. VIRUSES 11/08/20 Tomato Brown Rugose Fruit Virus Contributes to Enhanced Pepino Mosaic Virus Titers in Tomato Plants. JOUE 12/08/20 REGLEMENT D'EXÉCUTION (UE) 2020/1191 DE LA COMMISSION du 11 août 2020 établissant des mesures destinées à éviter l'introduction et la dissémination du virus du fruit rugueux brun de la tomate dans l'Union et abrogeant la décision d'exécution. BSPP 01/04/20 First report of Tomato brown rugose fruit virus infecting sweet pepper in Italy. BIORXIV 02/06/20 Real-time tracking of Tomato brown rugose fruit virus (ToBRFV) outbreaks in the Netherlands using Nextstrain. HORTI DAILY 11/05/20 5 washing processes tested, virus removed 5x - Washing crates to beat ToBRFV.
BSPP 30/04/20 First report of Tomato brown rugose fruit virus on tomato in Egypt. PARLEMENT EUROPEEN - Réponse à question E-000923/2020 Deadly virus ravages EU tomato crops. BIORXIV 02/03/20 Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection of Tomato brown rugose fruit virus (ToBRFV) BIORXIV 02/03/20 Development and evaluation of a loop-mediated isothermal amplification (LAMP) assay for the detection of Tomato brown rugose fruit virus (ToBRFV) SERVICE PUBLIC 25/02/20 Tomates, piments, poivrons : la menace d'un nouveau virus. LES ECHOS 18/02/20 Virus de la tomate : des plants britanniques à l'origine de la contamination en Bretagne. AFP 18/02/20 Ce que l’on sait du ToBRFV, le virus tueur de tomates. USDA - NOV 2019 - Tomato Brown Rugose Fruit Virus Fact Sheet.
RUTGERS_EDU 07/01/20 Understanding and Controlling Tomato Brown Rugose Fruit Virus. Error - Cookies Turned Off. PLANT PATHOLOGY 01/01/20 Evaluation of Datura stramonium and Nicandra physaloides as reservoirs of tomato severe rugose virus and whiteflies. PEERJ 17/10/19 Real-time reverse transcription polymerase chain reaction development for rapid detection of Tomato brown rugose fruit virus and comparison with other techniques.
MOTHERSHIP_SG 05/02/20 Devastating virus attacking tomatoes, peppers & chili plants in US, China & Europe. Hazera Seeds Ltd. via YOUTUBE 14/11/19 Hazera Official Training Video Tomato Brown Rugose Fruit Virus ToBRFV. FLORIDADAILY 14/10/19 Infected Mexican Tomatoes a Threat to Florida Agriculture ( tomato brown rugose fruit virus) CBP_GOV 18/11/19 CBP to begin inspections for Tomato Brown Rugose Fruit Virus. APS 22/04/19 First Report of Tomato Brown Rugose Fruit Virus Infecting Greenhouse Tomato in the United States.
AMHPAC via YOUTUBE 05/04/19 Webinar ToBRFV. AHDB Horticulture TV via YOUTUBE 30/10/19 Current Status and Perspectives of ToBRFV – a presentation from Aviv Dombrovsky (Volcani Institute) AHDB HORTICULTURE TV VIA YOUTUBE 22/11/19 Hygiene management for ToBRFV – a presentation from Jasper Verhoeven (Royal Brinkman)
HORTOINFO_ES 23/10/19 El peligroso virus ToBRFV, detectado en los invernaderos de Holanda. MIDI LIBRE 04/02/20 Un virus ravageur menace tomates et poivrons : l'Occitanie en première ligne. LIBERATION 04/02/20 Nouveau virus menaçant tomates et poivrons : «Le risque de dissémination en France est élevé» FRANCE INFO 04/02/20 "En cas de suspicion, il faut prévenir dès que possible les services de l'Etat" : un nouveau virus menace les plantations de tomates et préoccupe les autorités.
FRANCE 3 04/02/20 Virus de la tomate : Savéol, premier producteur en France, annonce des mesures de prévention. EUROPE 1 VIA YOUTUBE 03/02/20 Virus de la tomate : Philippe Reignault estime que "le niveau de vigilance doit être maximum" CNEWS 04/02/20 VIRUS TOBRFV : Y'AURA-T-IL DES TOMATES ET DES POIVRONS CET ÉTÉ ? AGROSCOPE - OCT 2019 - Fiche technique : Tomato brown rugose fruit virus (ToBRFV) AGRIRESEAU_NET - MAI 2019 - VIRUS DU FRUIT RUGUEUX BRUN DE LA TOMATE (ToBRFV) AFSCA 11/10/19 Virus du fruit rugueux de la tomate brune.
AFP 04/02/20 Alerte rouge sur les tomates : la France sous la menace du redoutable virus ToBRFV. 20MINUTES 04/02/20 Virus de la tomate : « Dans le scénario le plus sinistre, il y aurait une pénurie » ANSES - JANV 2020 - L’Anses met en garde contre un virus émergent qui affecte les plantes potagères. CBP_GOV 18/11/19 CBP to begin inspections for Tomato Brown Rugose Fruit Virus. Hazera Seeds Ltd. via YOUTUBE 14/11/19 Hazera Official Training Video Tomato Brown Rugose Fruit Virus ToBRFV. HORTOINFO_ES 23/10/19 El peligroso virus ToBRFV, detectado en los invernaderos de Holanda. FLORIDADAILY 14/10/19 Infected Mexican Tomatoes a Threat to Florida Agriculture ( tomato brown rugose fruit virus) AFSCA 11/10/19 Virus du fruit rugueux de la tomate brune. AGRIRESEAU_NET - MAI 2019 - VIRUS DU FRUIT RUGUEUX BRUN DE LA TOMATE (ToBRFV) VEGETABLE 15/04/19 Le risque du virus TOBRFV se précise. SMALLHOLDER 24/07/19 UK outbreak of Tomato Brown Rugose Fruit Virus.
Revistaalimentaria_es 25/07/19 Un nuevo virus amenaza a los cultivos de tomate y pimiento (Tomato brown rugose fruit virus, ToBRFV). PLOS 20/01/17 A New Israeli Tobamovirus Isolate Infects Tomato Plants Harboring Tm-22 Resistance Genes. PLOS 17/01/19 The bumblebee Bombus terrestris carries a primary inoculum of Tomato brown rugose fruit virus contributing to disease spread in tomatoes. OPENAGRAR_DE - 2019 - Tomato brown rugose fruit virus. NDRS_ORG_UK 08/01/19 First report of tomato brown rugose fruit virus infecting tomato in Germany. LINEA, ENERO 06/12/18 First report of Tomato brown rugose fruit virus (ToBRFV) in Michoacan, Mexico. INFOAGRO (MX) 15/08/18 Medidas de Prevención para nuevo Virus de Tomate (ToBRFV)
FRESH PLAZA 22/01/19 Interview to Prof Walter Davino - ToBRF Virus found in Sicily. DRAAF CORSE 08/04/19 BSV MARAICHAGE FLASH - TOMATO BROWN RUGOSE FRUIT VIRUS (TO BRFV) CESAVE Sonora via FACEBOOK 08/03/19 Transmisión del Seminario del Tomato Brown Rugose Fruit Virus (ToBRFV) desde el auditorio de la Junta Local de Sanidad Vegetal del Valle del Yaqui. CBC_CA 19/07/19 New virus threatening tomato crops in Essex County. BSPP 28/05/19 First report of Tomato brown rugose fruit virus on tomato in Turkey. BSPP 08/01/19 First report of Tomato brown rugose fruit virus infecting tomato in Germany. Archives of Virology February 2016, Volume 161 A new tobamovirus infecting tomato crops in Jordan. APS 09/07/19 First report of Tomato brown rugose fruit virus infecting tomato in China. AHDB Horticulture TV VIA YOUTUBE 30/04/19 Tomato Brown Rugose Fruit Virus (ToBRFV) update. AGROMIP_COM_GT - 2019 - XVI congreso international de manejo inetrado de plagas - Présentation : Tomato brown rugose fruit virus.