NATURE SCIENTIFIC REPORT 20/03/17 A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses. Assay speed and sensitivity We aimed to develop a rapid and reliable QUASR RT-LAMP assay for ZIKV, while relying on previously reported RT-LAMP assays for CHIKV and DENV17,19,22.
We first set about identifying an optimal primer set for ZIKV, using the LAMP-compatible SYTO intercalating dyes to perform real-time monitoring. We then adapted the optimal primer set to the target-specific QUASR endpoint detection technique. The mechanism and chemistry of QUASR-based RT-LAMP detection techniques has previously been described in detail22. At least one recent publication describes a different RT-LAMP assay for ZIKV utilizing leuco crystal violet indicator13. We prepared a ZIKV RNA standard from cultured ZIKV. (A) Positive amplification is detectable within 10 to 15 min using real time monitoring with SYTO 62, and reaction speed is comparable for RNA extracted from ZIKV isolates from Brazil, Honduras, and Puerto Rico (n = 6).
Int J Health Geogr. 2014; 13: 50. Modeling tools for dengue risk mapping - a systematic review. PLOS 21/02/17 Dengue virus antibody database: Systematically linking serotype-specificity with epitope mapping in dengue virus. Abstract Background A majority infections caused by dengue virus (DENV) are asymptomatic, but a higher incidence of severe illness, such as dengue hemorrhagic fever, is associated with secondary infections, suggesting that pre-existing immunity plays a central role in dengue pathogenesis.
DISEASES - 2016 - Early Detection for Dengue Using Local Indicator of Spatial Association (LISA) Analysis. 1 Grupo de Análisis Funcional y Aplicaciones, Universidad EAFIT, Carrera 49 No. 7 Sur-50, Medellín 050022, Colombia 2 Grupo Biología y Control de Enfermedades Infecciosas—BCEI, Universidad de Antioquia, Calle 70 No. 52-21, Medellin 050010, Colombia * Author to whom correspondence should be addressed.
Received: 18 January 2016 / Revised: 21 March 2016 / Accepted: 25 March 2016 / Published: 29 March 2016 Dengue is a viral disease caused by a flavivirus that is transmitted by mosquitoes of the genus Aedes. There is currently no specific treatment or commercial vaccine for its control and prevention; therefore, mosquito population control is the only alternative for preventing the occurrence of dengue. For this reason, entomological surveillance is recommended by World Health Organization (WHO) to measure dengue risk in endemic areas; however, several works have shown that the current methodology (aedic indices) is not sufficient for predicting dengue.
MDPI and ACS Style AMA Style. CDC EID - Volume 20, Number 6—June 2014 Au sommaire: Dengue Virus Type 3, South Pacific Islands, 2013. Author affiliations: Institut Louis Malardé, Papeete, Tahiti, French Polynesia (V.
-M. Cao-Lormeau, C. PUBLIC HEALTH ENGLAND 08/07/14 Dengue and chikungunya 2013 data sees an increase in number of cases. Public Health England (PHE) has today (8 July 2014) published annual 2013 data for dengue fever and chikungunya.
In 2013, 541 cases of dengue fever were reported in travellers returning from dengue-affected countries, an overall increase of 58% compared to 2012. India and Thailand continue to be the most frequent countries of travel reported for dengue cases, although in 2013, there was also an increase in cases associated with travel to Barbados. There was also an increase in chikungunya with 24 cases reported in 2013 compared to 15 cases in 2012, mostly acquired in India and South East Asia. In December 2013, indigenously acquired chikungunya was first reported in St Martin, a French overseas territory in the Caribbean. By July 2014, at least 22 other islands and territories in the region had also reported indigenous chikungunya, this includes 4 cases reported in the UK associated with travel to the Caribbean.
Dr Jane Jones, travel and migrant health expert at PHE said: Ends. PLOS 30/09/14 Characteristics of a Dengue Outbreak in a Remote Pacific Island Chain – Republic of the Marshall Islands, 2011–2012. Abstract Dengue is a potentially fatal acute febrile illness caused by four mosquito-transmitted dengue viruses (DENV-1–4).
Although dengue outbreaks regularly occur in many regions of the Pacific, little is known about dengue in the Republic of the Marshall Islands (RMI). To better understand dengue in RMI, we investigated an explosive outbreak that began in October 2011. Suspected cases were reported to the Ministry of Health, serum specimens were tested with a dengue rapid diagnostic test (RDT), and confirmatory testing was performed using RT-PCR and IgM ELISA. Laboratory-positive cases were defined by detection of DENV nonstructural protein 1 by RDT, DENV nucleic acid by RT-PCR, or anti-DENV IgM antibody by RDT or ELISA.
EUROSURVEILLANCE 16/10/14 Concurrent outbreaks of dengue, chikungunya and Zika virus infections – an unprecedented epidemic wave of mosquito-borne viruses in the Pacific 2012–2014. Since January 2012, the Pacific Region has experienced 28 new documented outbreaks and circulation of dengue, chikungunya and Zika virus.
These mosquito-borne disease epidemics seem to become more frequent and diverse, and it is likely that this is only the early stages of a wave that will continue for several years. Improved surveillance and response measures are needed to mitigate the already heavy burden on island health systems and limit further spread to other parts of the world. Since January 2012, the Pacific is experiencing a high burden of mosquito-borne disease due to concurrent epidemics of dengue, chikungunya and Zika virus infections. So far over 120,000 people have been reported to be affected, a figure that is likely to substantially underestimate the real numbers due to underreporting. PLOS 20/11/14 Approaches to Refining Estimates of Global Burden and Economics of Dengue.
Results Dengue burden data and sources of variability Estimates of the disease and economic burden of dengue were derived by combining surveillance, clinical, and cost data.
Since dengue is a reportable disease in many endemic countries, the incidence of dengue in a population can be estimated initially from cases reported to the surveillance system. But because surveillance systems are not designed to capture all episodes of symptomatic dengue, relatively low reporting rates lead to conservative incidence estimates –. University of Oxford 23/08/13 A study of gravity-linked metapopulation models for the spatial spread of dengue fever. Liverpool School of Tropical Medicine - 2013 - Detecting and Responding to a Dengue Outbreak: Evaluation of Existing Strategies. School of Veterinary Medicine, University of São Paulo - 2013 - A Comparative Analysis of the Relative Efficacy of Vector-Contro. ECCMID - AVRIL 2013 - Poster : Dengue fever in travellers from endemic countries: the 2009-2011 experience at the INMI “L. Spall. Our systems have detected unusual traffic from your computer network. This page checks to see if it's really you sending the requests, and not a robot.
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URL: THE HEALTH EDUCATION MONOGRAPH SERIES - 2011 - Au sommaire: A review of selected practices for the control and prevention of Den.
Références OMS. PLOS 07/08/12 Refining the Global Spatial Limits of Dengue Virus Transmission by Evidence-Based Consensus. Abstract Background Dengue is a growing problem both in its geographical spread and in its intensity, and yet current global distribution remains highly uncertain.
Challenges in diagnosis and diagnostic methods as well as highly variable national health systems mean no single data source can reliably estimate the distribution of this disease. As such, there is a lack of agreement on national dengue status among international health organisations. Here we bring together all available information on dengue occurrence using a novel approach to produce an evidence consensus map of the disease range that highlights nations with an uncertain dengue status. Methods/Principal Findings A baseline methodology was used to assess a range of evidence for each country. Conclusion Author Summary Figures.
Epidemiology and Infection, FirstView Article : pp 1-5 2011 Dengue: a newly emerging viral infection in Andaman and Nicobar Isla. Dengue en Amérique. Dengue en Afrique. Dengue en Europe. World distribution of dengue viruses and their mosquito. World_DengueTransmission_2006. World_DengueTransmission_Extension.