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FRONTIERS IN VETERINARY SCIENCE 30/05/17 A fast and inexpensive protocol for empirical verification of neutralizing epitopes in microbial toxins and enzymes (Clostridium perfringens) 1Veterinary Pathobiology, Texas A&M University, United States 2Poultry Science, Texas A&M University, United States 3Poultry Science, University of Arkansas, United States 4Animal Science, The Ohio State University, United States In vivo targeting of peptides to antigen-presenting cells by use of agonistic anti-CD40 monoclonal antibodies has been used successfully as an immune response enhancing strategy.

FRONTIERS IN VETERINARY SCIENCE 30/05/17 A fast and inexpensive protocol for empirical verification of neutralizing epitopes in microbial toxins and enzymes (Clostridium perfringens)

When tested in chickens, the antibody-guided platform was capable of inducing specific IgG production within one week post-immunization. However, use of this method beyond its initial conception as a vaccine delivery tool has not been fully exploited. UNIVERSIDAD DE COSTA RICA - 2005 - Thèse en ligne : Clostridium perfringens: deteccion y susceptibilidad antimicrobiana en diarreas nosocomiales en el hospital San Juan de Dios. Anaerobe Volume 44, April 2017, Prevalence and molecular typing of Clostridium perfringens in captive wildlife in India. Food Microbiology Volume 62, April 2017, Tolerance of Clostridium perfringens biofilms to disinfectants commonly used in the food industry. Highlights Clostridium perfringens has been shown to form mono-species biofilms.

Food Microbiology Volume 62, April 2017, Tolerance of Clostridium perfringens biofilms to disinfectants commonly used in the food industry

C. perfringens mono-species biofilms were able to protect the bacterial cells from the action of commonly used disinfectants. Sodium hypochlorite solution was shown to be effective on C. perfringens biofilms. Mono-species biofilms were more tolerant to disinfectants than the mixed biofilms. Abstract. AEM 21/10/16 Inactivation Strategies for Clostridium perfringens Spores and Vegetative Cells. TOXINS - 2016 - Regulation of Toxin Production in Clostridium perfringens. TOXINS 19/11/16 Clostridium perfringens Sialidases: Potential Contributors to Intestinal Pathogenesis and Therapeutic Targets. OREGON STATE UNIVERSITY 01/07/15 Thèse en ligne : Inactivation strategy for Clostridium perfringens spores adhered onto stainless steel surfaces. OREGON STATE UNIVERSITY - 2015 - Survival of Clostridium difficile Spores at Low Temperatures.

Int J Clin Exp Pathol. 2015 Jan 1;8(1):569-77. eCollection 2015. Epidemiological and pathobiological profiles of Clostridium perfringens infections: review of consecutive series of 33 cases over a 13-year period. IOWA STATE UNIVERSITY - 2015 - Thèse en ligne : Efficacy of decreased nitrite concentrations on Clostridium perfringens outgrowth during an Appendix B cooling cycle for ready-to-eat meats. Abstract Increased popularity of natural and organic processed meats can be attributed to the growing consumer demand of preservative-free foods.

IOWA STATE UNIVERSITY - 2015 - Thèse en ligne : Efficacy of decreased nitrite concentrations on Clostridium perfringens outgrowth during an Appendix B cooling cycle for ready-to-eat meats

In order to meet consumer demand, processors have begun using celery juice concentrate in place of sodium nitrite creating no-nitrate-no-nitrite added meat products while maintaining the characteristics unique to conventionally cured processed meats. Natural cures typically have lower ingoing nitrite concentrations which could allow for increased pathogen growth, such as Clostridium perfringens, during cooling as required by USDA, FSIS Appendix B. The objective of this study was to investigate the implications associated with reduced nitrite concentrations and their ability to inhibit C. perfringens outgrowth during a 15 hour cooling cycle.

Nitrite treatments of 0 ppm, 50 ppm, and 100 ppm were tested in a broth system inoculated with a three-strain C. perfringens cocktail and treated with a 15 hour stabilization cycle. Recommended Citation. Veterinary Microbiology Volume 174, Issues 3–4, 5 December 2014, Prevalence of Clostridium perfringens, Clostridium perfringens enterotoxin and dysbiosis in fecal samples of dogs with diarrhea.

Anaerobe Volume 33, June 2015, Clostridium perfringens: A review of enteric diseases in dogs, cats and wild animals. IOWA STATE UNIVERSITY - 1970 - Thèse en ligne : sporulation and germination of spores of clostridium perfringens. Cienc. Rural vol.45 no.6 Santa Maria June 2015 Epub Mar 17, 2015 Clostridium perfringens: a review of the disease in pigs, horses and broiler chickens. APPLIED AND ENVIRONMENTAL MICROBIOLOGY - 1981 - Survival of clostridial spores in animal tissues.

FOODBORNE PATHOGENS AND DISEASE Volume 7, Number 2, 2010 Potential for Growth of Clostridium perfringens from Spores in Pork Scrapple During Cooling. UNIVERSITY OF WISCONSIN - MARS 2002 - Survival and Growth of Clostridium perfringens during the Cooling Step of Thermal Processing of Meat Products. You are using an outdated version of Firefox which is not supported by ResearchGate anymore.

UNIVERSITY OF WISCONSIN - MARS 2002 - Survival and Growth of Clostridium perfringens during the Cooling Step of Thermal Processing of Meat Products

For a faster, safer browsing experience, upgrade your browser now. <div class="c-box-warning full-width-element" style="text-align: center; "><div style="margin: auto; padding:10px;" class="container"><b>For full functionality of ResearchGate it is necessary to enable JavaScript. Here are the <a href=" rel="nofollow" target="_blank"> instructions how to enable JavaScript in your web browser</a>. </b></div></div> Article. The Open Toxinology Journal, 2010, 3, 24-42 Clostridium Perfringens Toxins Involved in Mammalian Veterinary Diseases.

UNIVERSITY OF SOUTHAMPTON - MAI 2004 - Desk studies on feasibility of horizontal standard rapid methods for detection of Clostridium perfringens and enterococci in sludges, soil, soil improvers, growing media and biowastes. Use of quantitative PCR of functional genes to monitor coliforms and enteric bacteria in a constructed, urban wetland DM IV, KL Lowe - bios, 2012 - BioOne ... doi: 10.1021/es903496m CrossRef, PubMed.

UNIVERSITY OF SOUTHAMPTON - MAI 2004 - Desk studies on feasibility of horizontal standard rapid methods for detection of Clostridium perfringens and enterococci in sludges, soil, soil improvers, growing media and biowastes

International Journal of Microbiology Research 14/08/12 TOXINOTYPING AND ANTIMICROBIAL SUSCEPTIBILITY OF Clostridium perfringens ISOLATED FROM BROILER CHICKENS WITH NECROTIC ENTERITIS. Rev. sci. tech. Off. int. Epiz., 2013, 32(3), 841-8550 Antibiotic resistance of Clostridium perfringens isolates from broiler chickens in Egypt. Food Microbiology . 01/2007; Thermal inactivation of Bacillus cereus and Clostridium perfringens vegetative cells and spores in pork luncheon roll. Department of Food and Environmental Hygiene - Faculty of Veterinary Medicine - University of Helsinki, Finland - 2008 -  DIAGNO.

J Food Prot. 2013 Jan;76(1):65-71. Growth potential of Clostridium perfringens from spores in acidified beef, pork, and poultry. International Journal of Microbiological Research (IJMR) Volume 3 Number (3), 2012 Prevalence of Clostridium perfringens Alpha T. African Journal of Microbiology Research 12/07/13 Identification of four major toxins of Clostridium perfringens recovered from. J Med Microbiol. 2013 Mar;62(Pt 3):457-66. Prevalence and characterization of Clostridium perfringens from the faecal microbiota. J Public Health Manag Pract. 2013 Jun 11. Use of an Online Survey During an Outbreak of Clostridium perfringens in a Retirement.

J Public Health Manag Pract. 2013 Jun 11. Use of an Online Survey During an Outbreak of Clostridium perfringens in a Retirement Community-Arizona, 2012. – guatemalt

Food Control, Available online 10/07/13 Probabilistic evaluation of Clostridium perfringens potential growth in order to validat. Highlights Method for assessing the Clostridium perfringens growth during cooling operations.

Food Control, Available online 10/07/13 Probabilistic evaluation of Clostridium perfringens potential growth in order to validat

Probabilistic calculations combining thermal equations and microbiological models. Thermal equations parameters assessed by tests carried out in a kitchen. Possibility of using this method to validate cooling operations in catering. Abstract The cook-chill operation, a widely used process within central cooking facilities, implies that cooked dishes have to be quickly cooled down immediately after cooking in order to prevent any food-borne diseases due to Clostridium perfringens. This document presents an experimental method to validate the sanitary efficiency of the cooling process. The tests consisted in monitoring food temperature at the center of the containers from the end of cooking until the beginning of cold room storage.

From the monitored time–temperature profiles, thermal cooling equations were established for modeling. Keywords Copyright © 2013 Elsevier Ltd. MICROBIOLOGY AUSTRALIA - MAI 2013 - Food Safety. Au sommaire:Food poisoning due to Clostridium perfringens. Download PDF Article Published: 13 May 2013 “No other food poisoning bacterium is so uniquely poised to take advantage of the slackness of human beings involved in food preparation.”

MICROBIOLOGY AUSTRALIA - MAI 2013 - Food Safety. Au sommaire:Food poisoning due to Clostridium perfringens

(John Bates – Food Poisoning lectures). Anaerobe Volume 21, June 2013, Pages 67–68 Presence of Clostridium perfringens in retail chicken livers. Food Microbiology Volume 34, Issue 2, June 2013, Pages 328–336 Inactivation strategy for Clostridium perfringens spores adhered. Abstract The contamination of enterotoxigenic Clostridium perfringens spores on food contact surfaces posses a serious concern to food industry due to their high resistance to various preservation methods typically applied to control foodborne pathogens.

Food Microbiology Volume 34, Issue 2, June 2013, Pages 328–336 Inactivation strategy for Clostridium perfringens spores adhered

In this study, we aimed to develop an strategy to inactivate C. perfringens spores on stainless steel (SS) surfaces by inducing spore germination and killing of germinated spores with commonly used disinfectants. The mixture of l-Asparagine and KCl (AK) induced maximum spore germination for all tested C. perfringens food poisoning (FP) and non-foodborne (NFB) isolates.

Incubation temperature had a major impact on C. perfringens spore germination, with 40 °C induced higher germination than room temperature (RT) (20 ± 2 °C). CDC MMWR 17/08/12 Fatal Foodborne Clostridium perfringens Illness at a State Psychiatric Hospital — Louisiana, 2010. August 17, 2012 / 61(32);605-608 Clostridium perfringens, the third most common cause of foodborne illness in the United States (1), most often causes a self-limited, diarrheal disease lasting 12–24 hours.

CDC MMWR 17/08/12 Fatal Foodborne Clostridium perfringens Illness at a State Psychiatric Hospital — Louisiana, 2010

Fatalities are very rare, occurring in <0.03% of cases (1). Death usually is caused by dehydration and occurs among the very young, the very old, and persons debilitated by illness (2). On May 7, 2010, 42 residents and 12 staff members at a Louisiana state psychiatric hospital experienced vomiting, abdominal cramps, and diarrhea. Within 24 hours, three patients had died. On May 8, a state psychiatric hospital contacted OPH to report three resident deaths that occurred following an outbreak of gastrointestinal illness in patients and staff members that began late in the evening of May 6. Can J Vet Res. 2012 Jul;76(3):161-5. Detection and characterization of Clostridium perfringens in the feces of healthy and diarr. Anaerobe Available online 27 June 2013 First isolation of Clostridium perfringens type E from a goat with diarrhea.

Abstract A 2-day-old goat died suddenly after the onset of severe diarrhea.

Anaerobe Available online 27 June 2013 First isolation of Clostridium perfringens type E from a goat with diarrhea

No specific gross lesions were observed except for a remarkably thin intestinal wall and watery intestinal contents. JMBFS / Das et al. 2012 : 2 (1) 162-174 GENOTYPING OF CLOSTRIDIUM PERFRINGENS FROM FRESH WATER FISH AND FISH PICKLES. SCIENTIFIC RESEARCH - JUNE 2013 - Some Characters of Cl. perfringens Isolated from Fresh and Marketed Processed Meat. Some Characters of Cl. perfringens Isolated from Fresh and Marketed Processed Meat This study was carried out a fresh meat immediately after slaughter and a marketed processed cattlemeat (sausage and minced meat).

A total of 530 meat samples were examined for the presence of Cl. perfringens, 423 were from fresh meat obtained immediately after slaughtering (108 cattle meat, 101 sheep meat, 100 camel meat and 114 buffaloe meat) and 107 processed meat (57 from sausage and 50 from minced meat). Cl. perfringens was isolated from 204 (48.2%) of fresh meat samples, 61 (56.5%) from cattle, 53 (52.5%) from sheep meat, 45 (45%) from camel meat and 45 (39.5%) from buffaloe meat. The isolation rate of Cl. perfringens was higher in processed meat, it was isolated from 68 (63.6%) of which 45 (78.9%) from sausage and 23 (46%) in minced meat. Cite this paper K. References. 2013 - Reported outbreaks of C. perfringens, 1992 - 2010. CDC 03/05/13 Clostridium perfringens (FAQ) FDA - Bad bug book - Clostridium perfringens. Bad Bug Book:Foodborne Pathogenic Microorganisms and Natural Toxins HandbookClostridium perfringens 1.

Name of the Organism: Clostridium perfringens Clostridium perfringens is an anaerobic, Gram-positive, sporeforming rod (anaerobic means unable to grow in the presence of free oxygen). It is widely distributed in the environment and frequently occurs in the intestines of humans and many domestic and feral animals. 2.

Perfringens food poisoning is the term used to describe the common foodborne illness caused by C. perfringens. 3. The common form of perfringens poisoning is characterized by intense abdominal cramps and diarrhea which begin 8-22 hours after consumption of foods containing large numbers of those C. perfringens bacteria capable of producing the food poisoning toxin. Necrotic enteritis (pig-bel) caused by C. perfringens is often fatal. Infective dose--The symptoms are caused by ingestion of large numbers (greater than 10 to the 8th) vegetative cells.

RIVM 13/07/11 Clostridium perfringens associated with food borne disease : final report. Bull Vet Inst Pulawy 54, 571-576, 2010 OCCURRENCE OF CLOSTRIDIUM PERFRINGENS IN FOOD CHAIN. Full text The occurrence of Clostridium perfringens in particular links of food chain and their toxic potential was studied. Compound feeding stuffs, poultry, swine, and bovine faeces, food of animal origin, and human faeces were examined microbiologically. The isolated anaerobes were analysed for presence of toxin genes (cpa, cpb, cpb2, etx, iap, and cpe). RIVM 25/03/10 Heat sensitivity of Clostridium perfringens. J. Venom. Anim. Toxins incl. Trop. Dis vol.18 no.1 Botucatu 2012 Characterization of Clostridium perfringens isolated from mamma. Characterization of Clostridium perfringens isolated from mammals and birds from Guwahati city, India Rahman Mafruza S; Sharma RK; Borah P; Chakraborty A; Devi Mandakini RK; Longjam N Department of Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, India Correspondence to Of the 102 samples collected from mammals and birds, both domestic and captive wild, 48 were found to be positive for Clostridium perfringens.

Most of the mammal isolates (84.38%) appeared to have been collected from clinically affected animals, while 33.33% of the bird samples were from clinically affected and 21.43% from apparently healthy birds infected with C. perfringens. Key words: Clostridium perfringens, PCR, α toxin, cpa gene, polymerase chain reaction.

Different cases of acute enteritis or fatal enterotoxemia have been reported in several animal species. International Journal of Microbiological Research 2 (3): 196-203, 2011 Incidence of Clostridium perfringens in Meat Products at. BMC Veterinary Research 2012, 8:94 Presence and molecular characterization of Clostridium difficile and Clostridium perfringens.