Int. J. Mol. Sci. 05/11/20 A Novel Multiplex RT-PCR Assay for Simultaneous Detection of Dengue and Chikungunya Viruses. BMC Res Notes. 2020 Oct 12; Analysis of the virus propagation profile of 14 dengue virus isolates in Aedes albopictus C6/36 cells. Materials and methods Cell lines and cell culture conditions C6/36 cells (ATCC®CRL-1660™) were cultured at 28 °C in minimum essential medium (MEM, Thermo Fisher Scientific, Waltham, MA).
MEM was supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific, Waltham, MA) and 100 units/ml of penicillin/streptomycin solution (Pen/Strep, Merck KGaA, Darmstadt, Germany) and ambient CO2. LLC-MK2 cells (ATCC®CCL-7™) were cultured at 37 °C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific, Waltham, MA). The International Society for Neglected Tropical Diseases via youtube 02/10/20 ISNTD UNGA75 Dengue Side Meeting SESSION 3: DRIVING INNOVATION WITHIN VECTOR CONTROL. Viruses. 2020 Sep 29; Releasing Intracellular NS1 from Mosquito Cells for the Detection of Dengue Virus-Infected Mosquitoes. Dengue virus (DENV), the pathogen that causes dengue fever, is mainly transmitted by Aedes aegypti.
Surveillance of infected mosquitoes is a major component of integrated mosquito control methods for reducing the risk of vector-born disease outbreaks. However, a specialized rapid test for DENV detection in mosquitoes is not currently available. Utilizing immunoblotting, we found that the secretion of NS1 from both a DENV-infected mosquito cell line and mosquito bodies was below the detection threshold. However, when Triton X-100 was used to lyse infected mosquitoes, intracellular NS1 was released, and could then be effectively detected by the NS1 rapid test. J Infect Public Health. 2020 Feb;13 Diagnosis of severe dengue: Challenges, needs and opportunities.
Introduction Dengue is a mosquito-borne viral disease endemic in tropical and subtropical regions around the world.
Dengue virus (DENV) is responsible for causing dengue fever (DF), which is an acute febrile illness that is often self-limiting. A small percentage of those who contracted the infection however, developed the severe forms of the infection which could result in severe intravascular leakages, multiple organ failures and deaths . Approximately 3.9 billion people living in 128 countries are at risk of contracting dengue . An estimated 50–100 million cases of apparent infections occurred worldwide annually , which resulted in 22,000 deaths .
THE SCIENTIST 27/08/20 Zika Infection Increases Risk of Severe Dengue Fever. The 2015–16 Zika virus epidemic that spread through Central and South America was followed last year by a surge in dengue virus cases.
While this unfortunate series of diseases battered the region’s populace, it gave scientists, who had been following a cohort of several thousand children in the area, the opportunity to study how these two flaviviruses might jointly affect the immune system. That research has revealed that kids infected with dengue virus are more likely to suffer worse symptoms if they had previously been infected with Zika than if they hadn’t. The findings, reported in Science today (August 27), indicate that antibody-dependent enhancement—a phenomenon known for making a second infection with a virus worse than the first—is not limited to influencing infections by the same pathogen. This raises concerns that such cross-species effects may occur for other types of viruses—including coronaviruses—and may impact vaccine safety, scientists say.
INTECH 11/07/20 Dengue Virus and the Relationship with MicroRNAs. 1.
Résumé traduit : La dengue est une maladie fébrile aiguë causée par un virus du genre Flavivirus, famille des Flaviviridae, endémique dans les régions tropicales du globe. L'agent est un virus à ARN simple brin, classé en quatre sérotypes distincts du virus de la dengue (DENV): DENV-1, DENV-2, DENV-3 et DENV-4. Les réponses immunitaires innées et adaptatives de l'hôte jouent un rôle essentiel dans la détermination de l'histoire naturelle des infections virales, en particulier dans la dengue. Dans ce contexte, il a observé ces dernières années que la présence d'interférence ARN (ARNi) dans les processus d'infection virale est en augmentation, ainsi que la défense immunitaire. Le contexte des microARN (miARN) se démarque, car leur présence lors d'une infection virale, tant dans la réplication du virus que dans la défense contre ces infections, devient de plus en plus perceptible, d'où la nécessité de mieux comprendre le rôle de ces derniers. les petits ARN dans l'infection virale par le DENV et quelles sont leurs conséquences en aggravant les conséquences des patients touchés par cette maladie. – guatemalt
Introduction RNA interference (RNAi) is understood as the mechanism of gene silencing through transcription or post-transcription.
Post-transcription gene silencing (PTGS) operates through translational repression induced by microRNAs (miRNAs), from precursors transcribed in the nucleus . The silencing machinery by microRNAs directs mRNA to the P bodies present in the cytoplasm. They are deprived of the translation machinery and conserve proteins involved in the degradation of the target mRNA . The natural functions of RNAi and their related processes appear to be the protection of the genome against invasion by mobile genetic elements, such as viruses and transposons, as well as the functioning of eukaryotic organism development programs [3, 4]. These analyses led to the identification of proteins encoded by the host involved in gene silencing. 2. miRNA The first miRNA was described in 1993 and related to the regulation of larval development in Caenorhabditis elegans.
INTECH 21/05/20 Lessons Learned and Recent Advances in Dengue Research. 1.
Introduction Dengue is a single systematic and dynamic disease that includes severe and non-severe clinical manifestations . It is caused by any of the four dengue virus serotypes and it is transmitted by Aedes mosquito bites, being the main vector Aedes aegypti . Dengue can be maintained in an urban cycle which involves humans and it is a serious health problem worldwide . For the past decades, this disease has been spread alarmingly due to different factors like climate change, migration of people, tourism, lack of access to basic services, etc. [1, 2]. BIORXIV 12/05/20 Evolution of resistance to fluoroquinolones by dengue virus serotype 4 provides insight into mechanism of action and consequences for viral fitness. PLOS 11/05/20 From importation to autochthonous transmission: Drivers of chikungunya and dengue emergence in a temperate area. Abstract Background The global spread of Aedes albopictus has exposed new geographical areas to the risk of dengue and chikungunya virus transmission.
Several autochthonous transmission events have occurred in recent decades in Southern Europe and many indicators suggest that it will become more frequent in this region in the future. Environmental, socioeconomic and climatic factors are generally considered to trigger the emergence of these viruses. Accordingly, a greater knowledge of the determinants of this emergence in a European context is necessary to develop adapted surveillance and control strategies, and public health interventions.
Acta Tropica Available online 8 May 2020, Using the intrinsic growth rate of the mosquito population improves spatio-temporal dengue risk estimation. Achee et al., 2019 N.L.
Achee, J.P. Grieco, H. Vatandoost, G. Tropical Medicine and Infectious Disease 31/03/20 Special Issue "Epidemiology of Dengue: Past, Present and Future (Volume II)" Dear Colleagues, Dengue is widespread throughout tropical regions of the world, and its distribution is affected by climate, human behaviour (including urbanisation) and the competence and distribution of its mosquito vector.
The disease is endemic in many countries and episodically epidemic in still more. Worldwide, the disease is increasing in incidence with more than 300 million cases per year, and more than half the world's population is at risk. This Special Issue of the journal seeks contributions from authors who can advance our understanding of how dengue has emerged, the current extent and clinical impact of the epidemic and how it may behave in the future. We are especially interested in papers that describe the epidemic in the Pacific and Southeast Asian regions. Prof. SCIENTIFIC REPORTS 02/03/20 Silent circulation of dengue virus in Aedes albopictus (Diptera: Culicidae) resulting from natural vertical transmission. BIORXIV 07/02/20 Principles of dengue virus evolvability derived from genotype-fitness maps in human and mosquito cells. Spatially Adjusted Time-varying Reproductive Numbers: Understanding the Geographical Expansion of Urban Dengue Outbreaks.
NATURE 26/11/19 Modified mosquitoes reduce cases of dengue fever - Insects infected with a disease-inhibiting bacterium were released in communities in Asia and South America. Disease-carrying mosquitoes are on the defensive.
Cases of dengue fever, which is transmitted by the insects, plummeted in areas of Indonesia, Vietnam and Brazil in the months after researchers released Aedes aegypti mosquitoes that were modified to be resistant to dengue virus. The findings, presented on 21 November at the annual meeting of the American Society of Tropical Medicine and Hygiene in National Harbor, Maryland, come from experimental releases of mosquitoes that carry Wolbachia bacteria, which block the replication of mosquito-borne pathogens such as the dengue, chikungunya and Zika viruses. The Wolbachia infection then spreads through local mosquito populations. The efforts were led by the World Mosquito Program (WMP), a global consortium that is testing whether these releases can prevent mosquito-borne diseases.
BIORXIV 11/11/19 Administration of defective virus via bang-bang optimal control inhibits dengue transmission. VIROLOGY JOURNAL 29/10/19 Monoclonal antibody-based capture ELISA in the diagnosis of previous dengue infection. SCIENTIFIC REPORTS 17/09/19 Detection of dengue using PAMAM dendrimer integrated tapered optical fiber sensor. Characterization of PAMAM on TOF The presence of PAMAM was confirmed through Raman spectroscopy (Fig. 1). Vibrational modes representing C-C and C-H bonds were apparent in the spectrum. This included band peaks 610 cm−1, 835 cm−1, and 2930 cm−1 19,20,21. Aside from that, amino functional group terminals were also prominent here which were assigned to band peaks 1444 cm−1, 1595 cm−1, and 1303 cm−1 22,23.
DIAGNOSTICS 11/07/19 Visual Detection of Dengue-1 RNA Using Gold Nanoparticle-Based Lateral Flow Biosensor. Dengue is a rapidly spreading mosquito-borne viral disease. Early diagnosis is important for clinical screening, medical management, and disease surveillance. The objective of this study was to develop a colorimetric lateral flow biosensor (LFB) for the visual detection of dengue-1 RNA using dextrin-capped gold nanoparticle (AuNP) as label. The detection was based on nucleic acid sandwich-type hybridization among AuNP-labeled DNA reporter probe, dengue-1 target RNA, and dengue-1 specific DNA capture probe immobilized on the nitrocellulose membrane. Positive test generated a red test line on the LFB strip, which enabled visual detection. The optimized biosensor has a cut-off value of 0.01 µM using synthetic dengue-1 target. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
BMC Public Health 16/09/19 Prevalence and laboratory analysis of malaria and dengue co-infection: a systematic review and meta-analysis. Search results and study characteristics A total of 2811 citations were identified from PubMed (n = 1382) and Web of Science (n = 1429) databases, of which 542 articles were found to be duplicates. Of the 2269 articles screened, there were 1202 articles excluded after reading the titles and abstracts due to irrelevant records. Of the 1067 articles screened thereafter, 577 articles were excluded due to a lack of full text. Of the 490 full text articles reviewed, 443 were excluded. Of the 47 full text articles reviewed, 32 articles were excluded due to a lack of information on co-infection. PRISMA diagram. Thirteen studies can be used to compare the odds of Plasmodium sp. infection.
NATURE MICROBIOLOGY 10/06/19 The current and future global distribution and population at risk of dengue. To create the 2015 map of environmental suitability for dengue occurrence, we applied an ecological niche modelling approach to establish a multivariate empirical relationship between the probability of dengue presence and the environmental conditions sampled at disease occurrence locations.
Specifically, we used an ensemble boosted regression tree (BRT) methodology, which requires the generation of: (1) a comprehensive compendium of the locations of disease reports in humans; (2) a set of background points representing environmental conditions across the area of interest; and (3) a set of global gridded layers of environmental and socioeconomic covariates known or hypothesized to affect dengue transmission.
The resulting model produces a 5 × 5-km spatial resolution global map of environmental suitability for dengue presence. Ensemble BRT approach The BRT modelling procedure combines regression trees21 with gradient boosting22. GEOSPATIAL HEALTH - 2018 - Effects of socio-economic and environmental factors on the spatial heterogeneity of dengue fever investigated at a fine scale. Yubing Qu State Key Laboratory of Resources and Environmental Information System, Institute of Geographical Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing; Institute of Geodesy and Geophysics, Chinese Academy of Sciences, Wuhan, Hubei, China.
NATURE 29/03/19 Structure mapping of dengue and Zika viruses reveals functional long-range interactions. BMC INFECTIOUS DISEASES 21/03/19 Prediction of dengue outbreaks based on disease surveillance, meteorological and socio-economic data. Dengue, a mosquito-borne viral disease, is caused by four distinct, but closely related, serotypes of the virus [1, 2]. NATURE 18/03/19 Effects of infection history on dengue virus infection and pathogenicity.
Understanding the dynamics of DENV infection and risk modifiers requires knowledge about individual-level infection history, which is usually not fully observed even in pediatric cohort studies. To address this challenge, we developed a statistical approach that combines individual-level data from prospective serological cohorts with surveillance data, which facilitates joint inference of missing or left-censored infection history and epidemiological parameters. Using this approach, we estimated serotype-specific annual risk of infection for children in Managua, Nicaragua, from 1995 to 2009. FAPESP 07/11/18 A biomarker that detects dengue hemorrhagic fever is discovered. Elucidating the mechanism by which dengue virus infection develops into its hemorrhagic form could lead to new treatments and vaccines.
The research was done in Brazil with support from FAPESP (photo: James Gathany / CDC) November 07, 2018. BMC Public Health. 2017; 17(Suppl 1): 426. Coverage and beliefs about temephos application for control of dengue vectors and impact of a community-based prevention intervention: secondary analysis from the Camino Verde trial in Mexico. VIRUSES 18/10/18 Time to Harmonize Dengue Nomenclature and Classification. Int. J. Environ. Res. Public Health 2018, 15(9), 2018; The Sensitivity, Specificity and Accuracy of Warning Signs in Predicting Severe Dengue, the Severe Dengue Prevalence and Its Associated Factors. Int. J. Environ. Res. Public Health 29/08/18 Spatial Clustering of Dengue Fever Incidence and Its Association with Surrounding Greenness. With more than 58,000 cases reported by the country’s Centers for Disease Control, the dengue outbreaks from 2014 to 2015 seriously impacted the southern part of Taiwan. This study aims to assess the spatial autocorrelation of the dengue fever (DF) outbreak in southern Taiwan in 2014 and 2015, and to further understand the effects of green space (such as forests, farms, grass, and parks) allocation on DF.
In this study, two different greenness indexes were used. The first green metric, the normalized difference vegetation index (NDVI), was provided by the long-term NASA MODIS satellite NDVI database, which quantifies and represents the overall vegetation greenness. The latest 2013 land use survey GIS database completed by the National Land Surveying and Mapping Center was obtained to access another green metric, green land use in Taiwan. JOURNAL OF MEDICAL CARE REPORTS 08/08/18 Lessons learnt from managing a case of dengue hemorrhagic fever complicated with acute liver failure and acute kidney injury: a case report. CDC EID - AOUT 2018 - Distinguishing Zika and Dengue Viruses through Simple Clinical Assessment, Singapore. CDC EID - JULY 2018 - Use of Urea Wash ELISA to Distinguish Zika and Dengue Virus Infections.
Article Metrics. PLOS 10/05/18 Seasonal temperature variation influences climate suitability for dengue, chikungunya, and Zika transmission. Abstract Dengue, chikungunya, and Zika virus epidemics transmitted by Aedes aegypti mosquitoes have recently (re)emerged and spread throughout the Americas, Southeast Asia, the Pacific Islands, and elsewhere. Understanding how environmental conditions affect epidemic dynamics is critical for predicting and responding to the geographic and seasonal spread of disease. PLOS 03/05/18 Contributions from the silent majority dominate dengue virus transmission. Abstract Despite estimates that, each year, as many as 300 million dengue virus (DENV) infections result in either no perceptible symptoms (asymptomatic) or symptoms that are sufficiently mild to go undetected by surveillance systems (inapparent), it has been assumed that these infections contribute little to onward transmission. ASTMH - JANV 2018 - Dengue Fever Surveillance in India Using Text Mining in Public Media.
PLoS Negl Trop Dis. 2017 Jul 18;11(7): Dengue prediction by the web: Tweets are a useful tool for estimating and forecasting Dengue at country and city level. Abstract Background Infectious diseases are a leading threat to public health. NATURE 11/04/18 Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses. J Vector Borne Dis. 2017 Oct-Dec;54(4):301-310. Improving vector-borne pathogen surveillance: A laboratory-based study exploring the potential to detect dengue virus and malaria parasites in mosquito saliva.
Parasit Vectors. 2018 Feb 2;11(1):79. The spatial and temporal scales of local dengue virus transmission in natural settings: a retrospective analysis. Trop Biomed. 2014 Jun;31(2):286-96. Screening of antiviral activities in medicinal plants extracts against dengue virus using dengue NS2B-NS3 protease assay. Proc Natl Acad Sci U S A. 2017 Dec 26. Field- and clinically derived estimates of Wolbachia-mediated blocking of dengue virus transmission potential in Aedes aegypti mosquitoes.
Front Cell Infect Microbiol. 2017; 7: 336. Prevention and Control Strategies to Counter Dengue Virus Infection. VIRUSES 08/11/17 The 17th Rocky Mountain Virology Association Meeting. PLOS 07/09/17 Rapid and accurate interpretation of dengue diagnostics in the context of dengue vaccination implementation: Viewpoints and guidelines issued from an experts group consultation. J Theor Biol. 2016 Jul 7;400:138-53. Reconstruction of disease transmission rates: Applications to measles, dengue, and influenza. International Journal of Health Geographics 15/08/17 Relative risk estimation of dengue disease at small spatial scale. Int. J. Mol. Sci. 04/08/17 Co-Infection of Mosquitoes with Chikungunya and Dengue Viruses Reveals Modulation of the Replication of Both Viruses in Midguts and Salivary Glands of Aedes aegypti Mosquitoes. Procedia - Social and Behavioral Sciences Volume 234, 31 October 2016, Breeding Characteristics of Aedes Mosquitoes in Dengue Risk Area. PLOS 17/03/16 Is Dengue Vector Control Deficient in Effectiveness or Evidence?: Systematic Review and Meta-analysis.
BMC Microbiology 03/05/17 Simultaneous detection of chikungunya virus, dengue virus and human pathogenic Leptospira genomes using a multiplex TaqMan® assay. CDC EID - AVRIL 2017 – Au sommaire notamment : Biologic Evidence Required for Zika Disease Enhancement by Dengue Antibodies. Parasites & Vectors 11/03/17 How does the dengue vector mosquito Aedes albopictus respond to global warming? PLOS 21/12/16 The Impact of the Newly Licensed Dengue Vaccine in Endemic Countries. BMC 04/01/17 Evaluation of the importance of fever with respect to dengue prognosis according to the 2009 WHO classification: a retrospective study. MALARIA JOURNAL 03/01/17 The dangers of accepting a single diagnosis: case report of concurrent Plasmodium knowlesi malaria and dengue infection. Acta Tropica Volume 164, December 2016, Non-human primate antibody response to mosquito salivary proteins: Implications for dengue virus transmission in Puerto Rico.
Annals of Clinical Microbiology and Antimicrobials 22/07/16 ChikDenMaZika Syndrome: the challenge of diagnosing arboviral infections in the midst of concurrent epidemics. Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses - Volume 22, Number 7—July 2016. Cell report - JUIN 2016 - Identification of Zika Virus and Dengue Virus Dependency Factors using Functional Genomics. BIORXIV 24/03/16 Comparative analysis of dengue and Zika outbreaks reveals differences by setting and virus. BMC 03/03/16 Co-distribution and co-infection of chikungunya and dengue viruses.
GENOME BIOLOGY 02/03/16 Genomic approaches for understanding dengue: insights from the virus, vector, and host. NATURE 01/03/16 Highly divergent dengue virus type 1 genotype sets a new distance record. Viruses 2014, 6, 683-7 Targeting Host Factors to Treat West Nile and Dengue Viral Infections. Molecules 2014, 19(6), 8151-8176 Natural Products as Source of Potential Dengue Antivirals. Int. J. Environ. Res. Public Health 2015, 12(1), 1-15 A Systematic Review and Meta-Analysis of Dengue Risk with Temperature Change. Int. J. Environ. Res. Public Health 2015, 12(8), 9454-9474 Dengue Vaccines: A Perspective from the Point of View of Intellectual Property. PARASITES & VECTORS - 2014 - Global temperature constraints on Aedes aegypti and Ae. Albopictus persistence and competence for dengue virus transmission.
Int. J. Environ. Res. Public Health 2015, 12(1), 1-15 A Systematic Review and Meta-Analysis of Dengue Risk with Temperature Change.
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Site portail concernant la dengue. The Value of Educational Messages Embedded in a Community-Based Approach to Combat Dengue Fever: A Systematic Review and Meta Regression Analysis. PNAS - 2011 - Impact of daily temperature fluctuations on dengue virus transmission by Aedes aegypti. Experts Rev Vaccins 3(5) 2004 Dengue, one of the great emerging health challengs of the 21st Century. Mol. Biol. Evol. 20(10):1650-1658. 2003 Selection-Driven Evolution of Emergent Dengue Virus. The 20 Most-Frequently Cited Articles in Clin. Microbiol. Rev. as of January 1, 2009. Veterinary World - 2008 - Threat and strategic control of dengue infection in man. TROPIKA - OCT 2006 - Dengue Transmission Dynamics: Assessment.
EUROSURVEILLANCE Volume 6 - numéro 39, septembre 2002 - DengueNet - WHO's internet based system for the global surveillance of d.