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JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION 18/08/21 Laboratory Evaluation of the Rapid Analyte Measurement Platform Assay to Detect Dengue Virus in Mosquito Pools. We report the results of a laboratory sensitivity and specificity evaluation of the Rapid Analyte Measurement Platform (RAMP®) Dengue Virus (DENV) antigen detection assay, which is designed to detect all serotypes of DENV in mosquito pools.

JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION 18/08/21 Laboratory Evaluation of the Rapid Analyte Measurement Platform Assay to Detect Dengue Virus in Mosquito Pools

The RAMP DENV assay was able to detect geographically distinct strains of all 4 DENV serotypes in virus-spiked mosquito pools that contained at least 4.3 log10 plaque forming units/ml, although discrete sensitivity limits varied slightly for each serotype. The RAMP DENV assay also detected DENV 1–4 in mosquito pools containing a single infected mosquito and 24 laboratory-reared uninfected mosquitoes. No false positives were detected in negative control mosquito pools or in samples containing high titers of nontarget arboviruses. We found that while the kit-supplied RAMP buffer reduced the infectious titer of DENV, it did not completely inactivate all serotypes. Table 1. DENV is a biosafety level (BSL)-2 agent (U.S. Table 2. PLOS 07/06/21 Worldwide dynamic biogeography of zoonotic and anthroponotic dengue. Citation: Aliaga-Samanez A, Cobos-Mayo M, Real R, Segura M, Romero D, Fa JE, et al. (2021) Worldwide dynamic biogeography of zoonotic and anthroponotic dengue.

PLOS 07/06/21 Worldwide dynamic biogeography of zoonotic and anthroponotic dengue

PLoS Negl Trop Dis 15(6): e0009496. Editor: Michael R. Holbrook, NIAID Integrated Research Facility, UNITED STATES Received: October 30, 2020; Accepted: May 22, 2021; Published: June 7, 2021 Copyright: © 2021 Aliaga-Samanez et al. Data Availability: The data with the compendium of dengue cases from 2013 to 2017 that support the findings of this study are available in Dryad Digital Repository at with DOI: 10.5061/dryad.9w0vt4bfv. PLOS 03/06/21 Short Report: Adult Aedes abundance and risk of dengue transmission. Abstract Dengue is transmitted mainly by the adult female Aedes aegypti mosquito.

PLOS 03/06/21 Short Report: Adult Aedes abundance and risk of dengue transmission

However, little is known about the impact of adult Aedes abundance on the risk of dengue transmission. Here we analysed nationally representative dengue case and vector surveillance data collected from Singapore, to determine the effect of adult Aedes abundance on the risk of dengue transmission. A case was an area with active dengue transmission as indicated by the presence of dengue cluster. BMC 17/05/21 Structural landscape of the complete genomes of dengue virus serotypes and other viral hemorrhagic fevers. Structural properties of the DENV genomes Here, we analysed the secondary structure profiles of the complete genomes of more than 7000 ss-RNA viruses (Table 1; Methods: Source of viral genomes).

BMC 17/05/21 Structural landscape of the complete genomes of dengue virus serotypes and other viral hemorrhagic fevers

The structural profiles were generated using the CROSS algorithm, a fast and comprehensive alternative to profile the structural content (i.e., % of double-stranded nucleotides) of long and complex RNA molecules, such as viruses ([24]; see Methods: RNA secondary structure). To analyse DENV secondary structure, we selected one strain for each serotype, focusing on strains that were widely used in previous publications [25]. Mem. Inst. Oswaldo Cruz vol.113 no.11 Rio de Janeiro 2018 Epub Oct 29, 2018 Potential role of dengue virus, chikungunya virus and Zika virus in neurological diseases. PLOS 05/04/21 Probability of consolidation constrains novel serotype emergence in dengue fever virus. Abstract Since their first sequencing 40 years ago, Dengue virus (DENV) genotypes have shown extreme coherence regarding the serotype class they encode.

PLOS 05/04/21 Probability of consolidation constrains novel serotype emergence in dengue fever virus

Considering that DENV is a ribonucleic acid (RNA) virus with a high mutation rate, this behavior is intriguing. Here, we explore the effect of various parameters on likelihood of new serotype emergence. NATURE 01/03/21 Pan-serotype antiviral for dengue infection. Dengue virus (DENV) is a mosquito-borne flavivirus that exists in four distinct serotypes and can cause severe disease and death.

NATURE 01/03/21 Pan-serotype antiviral for dengue infection

Antiviral drugs to specifically treat DENV infections are not currently available. In a new study, researchers at Novartis report the development of a small molecule that is effective against all four DENV serotypes in vitro and shows promising efficacy and safety in animal models. MICROORGANISMS 03/02/21 Full-Length Genome and Partial Viral Genes Phylogenetic and Geographical Analysis of Dengue Serotype 3 Isolates. MDPI and ACS Style Amir, M.; Hussain, A.; Asif, M.; Ahmed, S.; Alam, H.; Moga, M.A.; Cocuz, M.E.; Marceanu, L.; Blidaru, A.

MICROORGANISMS 03/02/21 Full-Length Genome and Partial Viral Genes Phylogenetic and Geographical Analysis of Dengue Serotype 3 Isolates

Full-Length Genome and Partial Viral Genes Phylogenetic and Geographical Analysis of Dengue Serotype 3 Isolates. Microorganisms 2021, 9, 323. AMA Style. BIOMOLECULES 30/12/20 Potential Medicinal Plants for the Treatment of Dengue Fever and Severe Acute Respiratory Syndrome-Coronavirus. While dengue virus (DENV) infection imposes a serious challenge to the survival of humans worldwide, severe acute respiratory syndrome-coronavirus (SARS-CoV) remains the most devastating pandemic in human history.

BIOMOLECULES 30/12/20 Potential Medicinal Plants for the Treatment of Dengue Fever and Severe Acute Respiratory Syndrome-Coronavirus

A significant number of studies have shown that plant-derived substances could serve as potential candidates for the development of safe and efficacious remedies for combating these diseases. Different scientific databases were used to source for literature on plants used against these infections. ►▼ Show Figures Figure 1 This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. BIOMOLECULES 24/12/20 Antiviral Role of Phenolic Compounds against Dengue Virus: A Review. Phenolic compounds have been related to multiple biological activities, and the antiviral effect of these compounds has been demonstrated in several viral models of public health concern.

BIOMOLECULES 24/12/20 Antiviral Role of Phenolic Compounds against Dengue Virus: A Review

In this review, we show the antiviral role of phenolic compounds against dengue virus (DENV), the most widespread arbovirus globally that, after its re-emergence, has caused multiple epidemic outbreaks, especially in the last two years. Twenty phenolic compounds with anti-DENV activity are discussed, including the multiple mechanisms of action, such as those directed against viral particles or viral proteins, host proteins or pathways related to the productive replication viral cycle and the spread of the infection. Int. J. Mol. Sci. 05/11/20 A Novel Multiplex RT-PCR Assay for Simultaneous Detection of Dengue and Chikungunya Viruses. The goal of the study was to develop a specific, sensitive, and cost-effective molecular RT-PCR diagnostic assay for the rapid and simultaneous detection of the serotypes of dengue virus (DENV) and Chikungunya virus (CHIKV) from sera of suspected febrile patients.

Int. J. Mol. Sci. 05/11/20 A Novel Multiplex RT-PCR Assay for Simultaneous Detection of Dengue and Chikungunya Viruses

A single-tube, single-step multiplex RT-PCR (mRT-PCR) assay was designed for the detection of viral genomes from clinical and field samples. Specificity and sensitivity of the mRT-PCR assay were evaluated against six different combinations using two reverse transcriptases (AMV-RT and RT-Ace) and three DNA polymerases (LA-Taq, rTaq, and Tth). BMC Res Notes. 2020 Oct 12; Analysis of the virus propagation profile of 14 dengue virus isolates in Aedes albopictus C6/36 cells. Materials and methods Cell lines and cell culture conditions C6/36 cells (ATCC®CRL-1660™) were cultured at 28 °C in minimum essential medium (MEM, Thermo Fisher Scientific, Waltham, MA). MEM was supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific, Waltham, MA) and 100 units/ml of penicillin/streptomycin solution (Pen/Strep, Merck KGaA, Darmstadt, Germany) and ambient CO2. The International Society for Neglected Tropical Diseases via youtube 02/10/20 ISNTD UNGA75 Dengue Side Meeting SESSION 3: DRIVING INNOVATION WITHIN VECTOR CONTROL.

Viruses. 2020 Sep 29; Releasing Intracellular NS1 from Mosquito Cells for the Detection of Dengue Virus-Infected Mosquitoes. Dengue virus (DENV), the pathogen that causes dengue fever, is mainly transmitted by Aedes aegypti. Surveillance of infected mosquitoes is a major component of integrated mosquito control methods for reducing the risk of vector-born disease outbreaks. However, a specialized rapid test for DENV detection in mosquitoes is not currently available. Utilizing immunoblotting, we found that the secretion of NS1 from both a DENV-infected mosquito cell line and mosquito bodies was below the detection threshold.

However, when Triton X-100 was used to lyse infected mosquitoes, intracellular NS1 was released, and could then be effectively detected by the NS1 rapid test. J Infect Public Health. 2020 Feb;13 Diagnosis of severe dengue: Challenges, needs and opportunities. Introduction Dengue is a mosquito-borne viral disease endemic in tropical and subtropical regions around the world. Dengue virus (DENV) is responsible for causing dengue fever (DF), which is an acute febrile illness that is often self-limiting. A small percentage of those who contracted the infection however, developed the severe forms of the infection which could result in severe intravascular leakages, multiple organ failures and deaths [1]. Approximately 3.9 billion people living in 128 countries are at risk of contracting dengue [2]. THE SCIENTIST 27/08/20 Zika Infection Increases Risk of Severe Dengue Fever.

The 2015–16 Zika virus epidemic that spread through Central and South America was followed last year by a surge in dengue virus cases. While this unfortunate series of diseases battered the region’s populace, it gave scientists, who had been following a cohort of several thousand children in the area, the opportunity to study how these two flaviviruses might jointly affect the immune system. That research has revealed that kids infected with dengue virus are more likely to suffer worse symptoms if they had previously been infected with Zika than if they hadn’t. The findings, reported in Science today (August 27), indicate that antibody-dependent enhancement—a phenomenon known for making a second infection with a virus worse than the first—is not limited to influencing infections by the same pathogen.

This raises concerns that such cross-species effects may occur for other types of viruses—including coronaviruses—and may impact vaccine safety, scientists say. INTECH 11/07/20 Dengue Virus and the Relationship with MicroRNAs. 1. Introduction RNA interference (RNAi) is understood as the mechanism of gene silencing through transcription or post-transcription. Post-transcription gene silencing (PTGS) operates through translational repression induced by microRNAs (miRNAs), from precursors transcribed in the nucleus [1]. The silencing machinery by microRNAs directs mRNA to the P bodies present in the cytoplasm. They are deprived of the translation machinery and conserve proteins involved in the degradation of the target mRNA [2]. INTECH 21/05/20 Lessons Learned and Recent Advances in Dengue Research. BIORXIV 12/05/20 Evolution of resistance to fluoroquinolones by dengue virus serotype 4 provides insight into mechanism of action and consequences for viral fitness.

PLOS 11/05/20 From importation to autochthonous transmission: Drivers of chikungunya and dengue emergence in a temperate area. Acta Tropica Available online 8 May 2020, Using the intrinsic growth rate of the mosquito population improves spatio-temporal dengue risk estimation. Tropical Medicine and Infectious Disease 31/03/20 Special Issue "Epidemiology of Dengue: Past, Present and Future (Volume II)" SCIENTIFIC REPORTS 02/03/20 Silent circulation of dengue virus in Aedes albopictus (Diptera: Culicidae) resulting from natural vertical transmission. BIORXIV 07/02/20 Principles of dengue virus evolvability derived from genotype-fitness maps in human and mosquito cells. Spatially Adjusted Time-varying Reproductive Numbers: Understanding the Geographical Expansion of Urban Dengue Outbreaks.

NATURE 26/11/19 Modified mosquitoes reduce cases of dengue fever - Insects infected with a disease-inhibiting bacterium were released in communities in Asia and South America. Disease-carrying mosquitoes are on the defensive. Cases of dengue fever, which is transmitted by the insects, plummeted in areas of Indonesia, Vietnam and Brazil in the months after researchers released Aedes aegypti mosquitoes that were modified to be resistant to dengue virus. The findings, presented on 21 November at the annual meeting of the American Society of Tropical Medicine and Hygiene in National Harbor, Maryland, come from experimental releases of mosquitoes that carry Wolbachia bacteria, which block the replication of mosquito-borne pathogens such as the dengue, chikungunya and Zika viruses. The Wolbachia infection then spreads through local mosquito populations. The efforts were led by the World Mosquito Program (WMP), a global consortium that is testing whether these releases can prevent mosquito-borne diseases.

BIORXIV 11/11/19 Administration of defective virus via bang-bang optimal control inhibits dengue transmission. VIROLOGY JOURNAL 29/10/19 Monoclonal antibody-based capture ELISA in the diagnosis of previous dengue infection. SCIENTIFIC REPORTS 17/09/19 Detection of dengue using PAMAM dendrimer integrated tapered optical fiber sensor.

Characterization of PAMAM on TOF The presence of PAMAM was confirmed through Raman spectroscopy (Fig. 1). Vibrational modes representing C-C and C-H bonds were apparent in the spectrum. This included band peaks 610 cm−1, 835 cm−1, and 2930 cm−1 19,20,21. Aside from that, amino functional group terminals were also prominent here which were assigned to band peaks 1444 cm−1, 1595 cm−1, and 1303 cm−1 22,23. The observation was in accordance to the attachment of PAMAM as PAMAM molecules have many amino functional groups terminating their molecular branches. Raman spectrum after deposition of PAMAM. Characterization was continued with atomic force microscope (AFM) and field emission scanning electron microscopy (FESEM) analysis to determine the thickness, roughness, and conformation of the layers on each TOF profile tested Fig. 2(a) – (l).

AFM (left) and FESEM (right) images of PAMAM TOF: P1 = (a,b); P2 = (c,d); P3 = (e,f); P4 = (g,h); P5 = (i) and (j); P6 = (k,l). DIAGNOSTICS 11/07/19 Visual Detection of Dengue-1 RNA Using Gold Nanoparticle-Based Lateral Flow Biosensor. BMC Public Health 16/09/19 Prevalence and laboratory analysis of malaria and dengue co-infection: a systematic review and meta-analysis.

NATURE MICROBIOLOGY 10/06/19 The current and future global distribution and population at risk of dengue. GEOSPATIAL HEALTH - 2018 - Effects of socio-economic and environmental factors on the spatial heterogeneity of dengue fever investigated at a fine scale. NATURE 29/03/19 Structure mapping of dengue and Zika viruses reveals functional long-range interactions. BMC INFECTIOUS DISEASES 21/03/19 Prediction of dengue outbreaks based on disease surveillance, meteorological and socio-economic data. NATURE 18/03/19 Effects of infection history on dengue virus infection and pathogenicity. FAPESP 07/11/18 A biomarker that detects dengue hemorrhagic fever is discovered. BMC Public Health. 2017; 17(Suppl 1): 426. Coverage and beliefs about temephos application for control of dengue vectors and impact of a community-based prevention intervention: secondary analysis from the Camino Verde trial in Mexico.

VIRUSES 18/10/18 Time to Harmonize Dengue Nomenclature and Classification. Int. J. Environ. Res. Public Health 2018, 15(9), 2018; The Sensitivity, Specificity and Accuracy of Warning Signs in Predicting Severe Dengue, the Severe Dengue Prevalence and Its Associated Factors. Int. J. Environ. Res. Public Health 29/08/18 Spatial Clustering of Dengue Fever Incidence and Its Association with Surrounding Greenness. JOURNAL OF MEDICAL CARE REPORTS 08/08/18 Lessons learnt from managing a case of dengue hemorrhagic fever complicated with acute liver failure and acute kidney injury: a case report. CDC EID - AOUT 2018 - Distinguishing Zika and Dengue Viruses through Simple Clinical Assessment, Singapore. CDC EID - JULY 2018 - Use of Urea Wash ELISA to Distinguish Zika and Dengue Virus Infections. PLOS 10/05/18 Seasonal temperature variation influences climate suitability for dengue, chikungunya, and Zika transmission. PLOS 03/05/18 Contributions from the silent majority dominate dengue virus transmission.

ASTMH - JANV 2018 - Dengue Fever Surveillance in India Using Text Mining in Public Media. PLoS Negl Trop Dis. 2017 Jul 18;11(7): Dengue prediction by the web: Tweets are a useful tool for estimating and forecasting Dengue at country and city level. NATURE 11/04/18 Performance of the Trioplex real-time RT-PCR assay for detection of Zika, dengue, and chikungunya viruses. J Vector Borne Dis. 2017 Oct-Dec;54(4):301-310. Improving vector-borne pathogen surveillance: A laboratory-based study exploring the potential to detect dengue virus and malaria parasites in mosquito saliva.

Parasit Vectors. 2018 Feb 2;11(1):79. The spatial and temporal scales of local dengue virus transmission in natural settings: a retrospective analysis. Trop Biomed. 2014 Jun;31(2):286-96. Screening of antiviral activities in medicinal plants extracts against dengue virus using dengue NS2B-NS3 protease assay. Proc Natl Acad Sci U S A. 2017 Dec 26. Field- and clinically derived estimates of Wolbachia-mediated blocking of dengue virus transmission potential in Aedes aegypti mosquitoes.

Front Cell Infect Microbiol. 2017; 7: 336. Prevention and Control Strategies to Counter Dengue Virus Infection. VIRUSES 08/11/17 The 17th Rocky Mountain Virology Association Meeting. PLOS 07/09/17 Rapid and accurate interpretation of dengue diagnostics in the context of dengue vaccination implementation: Viewpoints and guidelines issued from an experts group consultation. J Theor Biol. 2016 Jul 7;400:138-53. Reconstruction of disease transmission rates: Applications to measles, dengue, and influenza. International Journal of Health Geographics 15/08/17 Relative risk estimation of dengue disease at small spatial scale. Int. J. Mol. Sci. 04/08/17 Co-Infection of Mosquitoes with Chikungunya and Dengue Viruses Reveals Modulation of the Replication of Both Viruses in Midguts and Salivary Glands of Aedes aegypti Mosquitoes. Procedia - Social and Behavioral Sciences Volume 234, 31 October 2016, Breeding Characteristics of Aedes Mosquitoes in Dengue Risk Area. PLOS 17/03/16 Is Dengue Vector Control Deficient in Effectiveness or Evidence?: Systematic Review and Meta-analysis.

BMC Microbiology 03/05/17 Simultaneous detection of chikungunya virus, dengue virus and human pathogenic Leptospira genomes using a multiplex TaqMan® assay. CDC EID - AVRIL 2017 – Au sommaire notamment : Biologic Evidence Required for Zika Disease Enhancement by Dengue Antibodies. Parasites & Vectors 11/03/17 How does the dengue vector mosquito Aedes albopictus respond to global warming? PLOS 21/12/16 The Impact of the Newly Licensed Dengue Vaccine in Endemic Countries. BMC 04/01/17 Evaluation of the importance of fever with respect to dengue prognosis according to the 2009 WHO classification: a retrospective study. MALARIA JOURNAL 03/01/17 The dangers of accepting a single diagnosis: case report of concurrent Plasmodium knowlesi malaria and dengue infection. Acta Tropica Volume 164, December 2016, Non-human primate antibody response to mosquito salivary proteins: Implications for dengue virus transmission in Puerto Rico. Annals of Clinical Microbiology and Antimicrobials 22/07/16 ChikDenMaZika Syndrome: the challenge of diagnosing arboviral infections in the midst of concurrent epidemics.

Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses - Volume 22, Number 7—July 2016. Cell report - JUIN 2016 - Identification of Zika Virus and Dengue Virus Dependency Factors using Functional Genomics. BIORXIV 24/03/16 Comparative analysis of dengue and Zika outbreaks reveals differences by setting and virus. BMC 03/03/16 Co-distribution and co-infection of chikungunya and dengue viruses.

GENOME BIOLOGY 02/03/16 Genomic approaches for understanding dengue: insights from the virus, vector, and host. NATURE 01/03/16 Highly divergent dengue virus type 1 genotype sets a new distance record. Viruses 2014, 6, 683-7 Targeting Host Factors to Treat West Nile and Dengue Viral Infections. Molecules 2014, 19(6), 8151-8176 Natural Products as Source of Potential Dengue Antivirals. Int. J. Environ. Res. Public Health 2015, 12(1), 1-15 A Systematic Review and Meta-Analysis of Dengue Risk with Temperature Change. Int. J. Environ. Res. Public Health 2015, 12(8), 9454-9474 Dengue Vaccines: A Perspective from the Point of View of Intellectual Property. PARASITES & VECTORS - 2014 - Global temperature constraints on Aedes aegypti and Ae. Albopictus persistence and competence for dengue virus transmission.

Int. J. Environ. Res. Public Health 2015, 12(1), 1-15 A Systematic Review and Meta-Analysis of Dengue Risk with Temperature Change.

Références OMS

Références PLOS. INFECTION ECOLOGY & EPIDEMIOLOGY - 2013 - Dengue viruses – an overview. International journal of Science Commerce and Humanities - OCT 2013 - Social Representations of Zoonosis Control Agents about de. FEMS Immunol Med Microbiol 53 (2008) 287–299 Vascular endothelium: the battle¢eld of dengueviruses. MEM INST OSWALDO CRUZ, RIO DE JANEIRO, 107(8) December 2012 Vector biology prospects in dengue research. International Journal of Epidemiology & Infection - 2013 - Temporal and Spatial Relationship by Environmental Factors as an Effe. CDC EID – JUILLET 2013 – au sommaire:Possible Cause of Liver Failure in Patient with Dengue Shock Syndrome. AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH 16/08/13 Challenges and future prospects for Dengue vector control.

FEMS Immunol Med Microbiol 47 (2006) Dengue and dengue haemorrhagic fever: implications of host genetics. Environ. Res. Lett. 7 (2012) 034003 (6pp) Potential impacts of climate change on the ecology of dengue and its mosquito vector t. INTECH - MARS 2012 - Implicating the Need for Serological Testing of Borna Disease Virus and Dengue Virus During Blood Transfusi. Tropical Medicine and International Health 14, 9 (2009) 1134-42 Spatial distribution and risk factors of dengue and Japanese enc. 17%20Aziz%20Shafie. Mathematical Biosciences - Modeling dengue outbreaks. Dengue fever. Dengue Virus Database.

Site portail concernant la dengue. The Value of Educational Messages Embedded in a Community-Based Approach to Combat Dengue Fever: A Systematic Review and Meta Regression Analysis.