MICROORGANISMS 17/11/21 Development of High-Throughput Multiplex Serology to Detect Serum Antibodies against Coxiella burnetii. The causative agent of Q fever, the bacterium Coxiella burnetii (C. burnetii), has gained increasing interest due to outbreak events and reports about it being a potential risk factor for the development of lymphomas.
In order to conduct large-scale studies for population monitoring and to investigate possible associations more closely, accurate and cost-effective high-throughput assays are highly desired. To address this need, nine C. burnetii proteins were expressed as recombinant antigens for multiplex serology. This technique enables the quantitative high-throughput detection of antibodies to multiple antigens simultaneously in a single reaction. Based on a reference group of 76 seropositive and 91 seronegative sera, three antigens were able to detect C. burnetii infections.
PATHOGENS 22/09/21 Q Fever Vaccine Development: Current Strategies and Future Considerations. Q fever is a zoonotic disease caused by the intracellular pathogen Coxiella burnetii.
This disease typically manifests as a self-limiting, febrile illness known as acute Q fever. Due to the aerosol transmissibility, environmental persistence, and infectivity of C. burnetii, this pathogen is a notable bioterrorism threat. Despite extensive efforts to develop next-generation human Q fever vaccines, only one vaccine, Q-Vax®, is commercially available. Q-Vax® is a phase I whole-cell vaccine, and its licensed use is limited to Australia, presumably due to the potential for a post-vaccination hypersensitivity response. Pre-clinical Q fever vaccine development is a major area of interest, and diverse approaches have been undertaken to develop an improved Q fever vaccine. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. VETERINARY SCIENCES 16/09/21 Serological Evidence of Human Infection with Coxiella burnetii after Occupational Exposure to Aborting Cattle.
Cattle are broadly deemed a source of Coxiella burnetii; however, evidence reinforcing their role in human infection is scarce.
Most published human Q fever outbreaks relate to exposure to small ruminants, notably goats. Anti-phase II C. burnetii IgG and IgM were measured by indirect fluorescent antibody tests in 27 farm and veterinary diagnostic laboratory workers to ascertain whether occupational exposure to cattle aborting due to C. burnetii was the probable source of exposure.
Four serological profiles were identified on the basis of anti-phase II IgG and IgM titres. Profile 1, characterised by high IgM levels and concurrent, lower IgG titres (3/27; 11.1%); Profile 2, with both isotypes with IgG titres higher than IgM (2/27; 7.4%); Profile 3 with only IgG phase II (5/27; 18.5%); and Profile 4, in which neither IgM nor IgG were detected (17/27; 63.0%). PARASITES & VECTORS 06/05/21 Real-time PCR biochip for on-site detection of Coxiella burnetii in ticks. Tick samples In total, 5644 larval, nymphal, and adult ticks from 408 pools were collected from livestock (cattle and horse) and wild animals (elk, roe deer, raccoon, badger, wild boar, and wild rabbit) from two provinces, Gangwon and Jeju, in Korea between August and November 2019.
The 235 pools collected from Gangwon Province were designated as 19M1–19M235, and the 173 samples collected from Jeju Province as 19T1–19T173. PATHOGENS 21/12/20 Validation of a Novel Commercial ELISA Test for the Detection of Antibodies against Coxiella burnetii. Q fever is a zoonosis caused by Coxiella burnetii, a Gram-negative pathogen with a complex life cycle and a high impact on public and animal health all over the world.
The symptoms are indistinguishable from those belonging to other diseases, and the disease could be symptomless. For these reasons, reliable laboratory tests are essential for an accurate diagnosis. The aim of this study was to validate a novel enzyme-linked immunosorbent assay (ELISA) test, named the Chorus Q Fever Phase II IgG and IgM Kit (DIESSE, Diagnostica Senese S.p.A), which is performed by an instrument named Chorus, a new device in medical diagnostics. This diagnostic test is employed for the detection of antibodies against C. burnetii Phase II antigens in acute disease. This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. JOURNAL OF DAIRY SCIENCE 17/04/19 Bayesian estimation of sensitivity and specificity of a PCR method to detect Coxiella burnetii in milk and vaginal secretions in sheep and goat samples.
FRONT. VET. SCI. 05/11/20 Coxiella burnetii in Dromedary Camels (Camelus dromedarius): A Possible Threat for Humans and Livestock in North Africa and the Near and Middle East? Introduction Q fever is a neglected zoonotic disease caused by bacteria (1, 2).
It is generally admitted that clones of Coxiella burnetii, the etiologic agent of Q fever, circulate in wildlife and infects domestic ruminants. Very few bacteria are required to initiate the infection process (3). Usually, humans become infected through the aerosol route during contact with C. burnetii–positive domestic animals or their products (2, 4). Infection of humans concerns first the farmers and other professionals that have contacts with animals (e.g., veterinarians), but epidemics have been reported in other social groups.
Regarding domestic ruminants, C. burnetii is responsible for epizooties with increased morbidity and mortality in livestock. PATHOGENS 25/10/20 Transstadial Transmission from Nymph to Adult of Coxiella burnetii by Naturally Infected Hyalomma lusitanicum. Coxiella burnetii (Derrick) Philip, the causative agent of Q fever, is mainly transmitted by aerosols, but ticks can also be a source of infection.
Transstadial and transovarial transmission of C. burnetii by Hyalomma lusitanicum (Koch) has been suggested. There is a close relationship between this tick species, wild animals and C. burnetii but the transmission in a natural environment has not been demonstrated. In this study, we collected 80 engorged nymphs of H. lusitanicum from red deer and wild rabbits. They moult to adults under laboratory conditions and we feed them artificially through silicone membranes after a preconditioning period. C. burnetii DNA was tested in ticks, blood and faeces samples using real-time PCR. ►▼ Show Figures Figure 1 This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. BMC Veterinary Research 28/08/20 Comparison of three serological tests for the detection of Coxiella burnetii specific antibodies in European wild rabbits.
Coxiella burnetii is the cause of Q fever or coxiellosis, a zoonotic bacterial disease of public health significance.
The life cycle of the C. burnetii include a wide range of host animal species, commonly farm livestocks, which act as reservoirs, transmitting the disease by shedding the bacterium into the environment. C. burnetii infections in cattle, sheep and goats are ususally asymptomatic but can cause production losses including abortions, infertility, still births, weak offsprings, metritis, mastitis and other reproductive disorders [1, 2]. C. burnetii can be shed in the urine, faeces, placental material and milk of infected animals . The organism can persist in the environment in a spore-like form for several months increasing the likelihood of infection of a new host .
Heavy rainfall and high winds speeds have been thought to cause the spores to aerosolise facilitating air-bourne spread and infections through inhalation . FRONT. VET. SCI. 10/07/20 Monitoring Coxiella burnetii Infection in Naturally Infected Dairy Sheep Flocks Throughout Four Lambing Seasons and Investigation of Viable Bacteria. Introduction Q fever is a widespread zoonosis caused by the intracellular bacterium Coxiella burnetii.
Résumé traduit de cette étude espagnole : Les résultats ont montré que l'infection peut rester active pendant plus de 5 ans si des mesures efficaces de contrôle et de biosécurité ne sont pas correctement mises en œuvre. – guatemalt
Goats and sheep are considered the main reservoir of C. burnetii, and both have a significant role as source of human infection (1, 2).
Q fever causes abortions in small ruminants (3), and once C. burnetii enters into a flock, infection spreads rapidly. Infected animals shed C. burnetii through birth products, vaginal fluids, feces, milk, and urine for several weeks after abortion or normal parturition (4–10), but the bacterial load shed by aborted animals is higher than that shed by those that deliver normally (9). Abortion rates due to C. burnetii are especially high in goats (up to 70–90%) (4, 11) but lower in sheep (usually below 6%) (12).
Coxiella burnetii shedding by infected animals, together with their movements in indoor animal premises, promotes the formation of contaminated aerosols. JOURNAL OF VETERINARY INTERNAL MEDICINE 06/08/18 Management of Coxiella burnetii infection in livestock populations and the associated zoonotic risk: A consensus statement. Abstract Infections caused by Coxiella burnetii, commonly referred to as coxiellosis when occurring in animals and Query fever when occurring in humans, are an important cause of abortions, decreased reproductive efficiency, and subclinical infections in ruminants.
The organism also represents an important zoonotic concern associated with its ability to aerosolize easily and its low infectious dose. Available diagnostic tests have limited sensitivity, which combined with the absence of treatment options in animals and limited approaches to prevention, result in difficulty managing this agent for optimal animal health and zoonotic disease outcomes. Rev. Bras. Parasitol. Vet. vol.28 no.2 Jaboticabal Apr./June 2019 Coxiella burnetii associated with BVDV (Bovine Viral Diarrhea Virus), BoHV (Bovine Herpesvirus), Leptospira spp., Neospora caninum, Toxoplasma gondii and Trypanosoma vivax in reproductive d. MICROMACHINES 27/06/19 Detection of Coxiella burnetii Using Silicon Microring Resonator in Patient Blood Plasma. One Health Volume 6, December 2018, Seroprevlance of Coxiella burnetii among abattoir and slaughterhouse workers: A meta-analysis. A. AbebePrevalence of Q fever infection in the Addis Ababa abattoir Ethiop. Med. J., 28 (1990), pp. 119-122 A.
New Microbiol., 34 (2011), pp. 219-224 M.R. J. VETERINARY WORLD 12/08/18 Molecular and immunological characterization of Hyalomma dromedarii and Hyalomma excavatum (Acari: Ixodidae) vectors of Q fever in camels. Research (Published online: 12-08-2018) 14. Molecular and immunological characterization of Hyalomma dromedarii and Hyalomma excavatum (Acari: Ixodidae) vectors of Q fever in camels Hend H.
A. ARCHIVES OF PUBLIC HEALTH 06/01/18 Farming, Q fever and public health: agricultural practices and beyond. The pleomorphic etiological agent of Q fever, C. burnetii is an intracellular bacterium that replicates in the parasitophorous vacuole of the parasitized cell (generally a macrophage or a trophoblast). The parasitic activity of this bacterium consists of building a finely orchestrated machinery that diverts the normal metabolism of the parasitized cell to become a surviving container devoted to the proliferation of the bacterium .
C. burnetii has a broad host range (with a role in dissemination or maintenance of the disease) and is found in unicellular organisms, invertebrates, birds and mammals . Domestic ruminants are the primary reservoir for human infection, and the majority of human epidemics are related to exposure to small ruminant (sheep and goats) infected products (placenta membranes, birth fluids, animal excretions or contaminated dust) [9, 10]. Transmission of infection from animals to humans is facilitated by the inhalation of contaminated aerosols. Bulgarian Journal of Veterinary Medicine - 2017 - ROLE OF COXIELLA BURNETII IN THE DEVELOPMENT OF FEVER OF UNKNOWN ORIGIN: А MINI REVIEW. CDC EID - MAI 2017 - Au sommaire notamment: No Such Thing as Chronic Q Fever. Author affiliations: French National Referral Center for Q Fever, Marseille, France Highlight and copy the desired format.
Abstract Modern diagnostic methods enable clinicians to look beyond a diagnosis of chronic Q fever and discern whether patients instead have persistent focalized Coxiella burnetii infection(s). Use of these methods and development of criteria to define and treat such infections, especially cardiovascular infections, will improve the prognosis for patients previously thought to have chronic Q fever. We read with interest the article by Kampschreur et al. (1), which in our opinion conveys a perspective that is incorrect concerning the diagnostic algorithm and treatment of Q fever. VETERINARY RESEARCH 05/04/16 Spread of Coxiella burnetii between dairy cattle herds in an enzootic region: modelling contributions of airborne transmission and trade. Our findings showed that airborne transmission and movement of cows both affected the regional spread of C. burnetii, but with different capacities.
On the one hand, airborne transmission had the ability to introduce C. burnetii in a large number of herds, but the generated outbreaks were generally predicted to be ephemeral and small. On the other hand, animal trade was predicted to result in only 8% of new infections; however, purchasing an infectious cow could instigate comparatively larger outbreaks. The differences in the impact of each transmission route on the intra-herd infection dynamics arose from the intrinsic nature of these transmission routes in spreading C. burnetii. Regardless the route, the first generated local infection was always a cow with health status I − as shown in Figure 2. Such a seronegative shedding cow is a transient shedder, which can become susceptible again. Irrespective of the benefits, mechanistic models are generally difficult to fit to data.
WAGENINGEN UNIVERSITY 06/05/14 Land-applied contaminated manure another source of spread of Q fever. Q fever is a zoonosis, an infectious disease that can be transferred from animals to humans. Previous research had shown that there is a spatial relationship between goat farms that were contaminated with Q fever and the incidence of the illness among humans. As expected more people became ill in the vicinity of contaminated farms that those living at a distance from these farms. Epidemiol. Infect. (2015), 143, 3316–3326. Q fever infection in dairy cattle herds: increased risk with high wind speed and low precipitation.
CDC EID - Volume 21, Number 7—July 2015. Chronic Q Fever Diagnosis—Consensus Guideline versus Expert Opinion ; Linda M. Kampschreur ( , Marjolijn C.A. Wegdam-Blans, Peter C. Wever, Nicole H.M. Renders, Corine E. CDC EID - Volume 21, Number 6—June 2015 - European Rabbits as Reservoir for Coxiella burnetii. Author affiliations: Spanish Wildlife Research Institute, Ciudad Real, Spain (D. González-Barrio, F. Ruiz-Fons); University of Trás-os-Montes e Alto Douro, Vila Real, Portugal (E. PLOS 15/05/15 The Recent Evolution of a Maternally-Inherited Endosymbiont of Ticks Led to the Emergence of the Q Fever Pathogen, Coxiella burnetii. Abstract. MICROBIOLOGY AUSTRALIA 18/09/13 Q fever: pets, vets and validating tests. Download PDF Article Published: 18 September 2013.
INTERDISCIPLINARY PERSPECTIVES ON INFECTIOUS DISEASES - 2012 - Q Fever: An Old but Still a Poorly Understood Disease. International Journal of Microbiology - 2011 - Q Fever: Current State of Knowledge and Perspectives of Research of a Neglected Z. PLOS 07/06/12 Q Fever and Pneumonia in an Area with a High Livestock Density: A Large Population-Based Study. Concerns about public health risks of intensive animal production in the Netherlands continue to rise, in particular related to outbreaks of infectious diseases. INTECH - AVRIL 2012 - Zoonosis. Au sommaire: Coxiella burnetii.
Edited by Jacob Lorenzo-Morales, ISBN 978-953-51-0479-7, 448 pages, Publisher: InTech, Chapters published April 04, 2012 under CC BY 3.0 licenseDOI: 10.5772/2125 Edited Volume Zoonotic diseases are mainly caused by bacterial, viral or parasitic agents although "unconventional agents" such as prions could also be involved in causing zoonotic diseases. Many of the zoonotic diseases are a public health concern but also affect the production of food of animal origin thus they could cause problems in international trade of animal-origin goods. RIVM 19/12/11 Detection of Coxiella burnetii DNA in animal and environmental matrices on non-dairy sheep farms. CDC EID – DEC 2011 - Proximity to Goat Farms and Coxiella burnetii Seroprevalence among Pregnant Women. Skip directly to local search Skip directly to A to Z list Skip directly to navigation Skip directly to site content Skip directly to page options CDC Home CDC 24/7: Saving Lives.
Protecting People. RIVM 25/10/11 Q fever: the answer is blowing in the wind : Detection of Coxiella burnetii in aerosols. MINLNV (PAYS BAS) - FEVRIER 2010 - Q fever conference. RIVM 31/05/11 A query for Coxiella in veterinary and environmental matrices. RIVM 31/05/11 Molecular detection and typing of Coxiella burnetii. RIVM - JANVIER 2010 - Q fever transmission to humans and local environmental conditions. BMC Infectious Diseases 2010, 10:69 The use of a geographic information system to identify a dairy goat farm as the most likely. BMC Veterinary Research 2011, 7:13 Q fever abortions in ruminants and associated on-farm risk factors in northern Cyprus.
ISBT Science Series - 2011 - XMRV, Q fever and other emerging infections: impact on management of blood safety.