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Tampere University (FI) - 2020 - Thèse en ligne: CRISPR-Cas9 based mutagenesis and gene silencing in zebrafish. Int. J. Mol. Sci. 20/07/20 CRISPR/Cas9: A Robust Genome-Editing Tool with Versatile Functions and Endless Application. Since a potential genome editing tool was first recognized in 2012 [1,2], the CRISPR/Cas9 system has been becoming a powerful and robust genome editing tool for gene function study and crop improvement.

Int. J. Mol. Sci. 20/07/20 CRISPR/Cas9: A Robust Genome-Editing Tool with Versatile Functions and Endless Application

Over the past decade, as new Cas enzymes have been identified, current Cas9 enzymes modified, and new bioinformatics tools developed, CRISPR/Cas9-based research has been developing extremely quickly. Particularly, the modification of Cas enzymes has significantly boosted the application potentials of CRISPR/Cas9 genome editing [3]. Although there are many other Cas enzymes identified and utilized in genome editing, the CRISPR/Cas9 system currently refers to any CRISPR/Cas system, including Cas12 and Cas13, majorly due to the fact that Cas9 is the first and most commonly used Cas enzyme in genome editing [4].

Among the 15 published papers, three are timely review papers. Among the 15 published papers, three are timely review papers. BIORXIV 02/04/20 A novel CRISPR-based malaria diagnostic capable of Plasmodium detection, speciation, and drug-resistance genotyping. NATURE 19/12/19 Multi-functional genome-wide CRISPR system for high throughput genotype–phenotype mapping. Strains, media, and cultivation conditions Escherichia coli strain NEB10β (New England Biolabs, Ipswich, MA) was used to maintain and amplify plasmids and recombinant strains were cultured at 37 °C in Luria broth medium containing 100 μg mL−1 ampicillin (LB/Amp).

NATURE 19/12/19 Multi-functional genome-wide CRISPR system for high throughput genotype–phenotype mapping

S. cerevisiae BY4742 was used as the host for genome-scale engineering of furfural tolerance and surface display of recombinant proteins. Yeast strains were cultivated in complex medium consisting of 2% peptone, 1% yeast extract, and 2% glucose (YPD) or synthetic complete medium consisting of 0.17% yeast nitrogen base, 0.1% mono-sodium glutamate, 0.077% CSM-URA, and 2% glucose (SED-URA) at 30 °C, 250 rpm.

When necessary, 200 μg mL−1 G418 (KSE Scientific, Durham, NC, USA) was supplemented. Plasmid and strain construction SNR52p-BsaI-BsaI-gRNA structural sequences-SUP4t8 were cloned into BsaI-free pRS426 to construct gRNA expression plasmids, including p426*-LbSgH for CRISPRa, p426*-SpSgH for CRISPRi, and p426*-SaSgH for CRISPRd. NATURE 15/01/20 The kill-switch for CRISPR that could make gene-editing safer. It started out as “sort of a stupid thing to do”, recalls Joe Bondy-Denomy, a microbiologist at the University of California, San Francisco.

NATURE 15/01/20 The kill-switch for CRISPR that could make gene-editing safer

As a graduate student in the early 2010s, he tried to infect bacteria with viruses that, on paper, shouldn’t have stood a chance. He knew that these viruses, or phages, were susceptible to CRISPR–Cas, the bacterial defence system that scientists have harnessed as a powerful tool for gene editing. And in most cases, he was right: the CRISPR machinery chopped the incoming phages into bits. But in a few instances, against the odds, the intruders survived. FRONT. SUSTAIN. FOOD SYST. 15/11/19 On-Farm Livestock Genome Editing Using Cutting Edge Reproductive Technologies. Introduction Preparing to feed a balanced and nutritious diet to the projected 9.7 billion people on the globe by 2050 will be one of the greatest challenges humanity has ever faced.

FRONT. SUSTAIN. FOOD SYST. 15/11/19 On-Farm Livestock Genome Editing Using Cutting Edge Reproductive Technologies

The FAO estimates demand for animal-based food products will increase by 70% in this time (Alexandratos and Bruinsma, 2012). Increasing reliance on plant-based diets and artificial meat production will contribute to improving food security and the sustainability of commercial agriculture, however outright omission of animal protein from human diets risks nutritional deficiencies and malnutrition, particularly in developing regions.

Meeting the anticipated increase in demand for animal food products in a way that has minimal impact on the environment and ensures high animal welfare standards will likely require the implementation of advanced technologies, including genome editing and cutting-edge reproductive technologies. FRONT. GENET. 03/09/19 Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era. Introduction Generating new and variable phenotypes via direct alteration of DNA sequences is an interesting idea that has sparked the curiosity of a wide spectrum of researchers over the past few decades.

FRONT. GENET. 03/09/19 Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era

Based on significant efforts, tremendous advances have been achieved in animal genetics and reproductive physiology. These have enabled what is now known as the genome-editing revolution that can be applied to generate gene-edited animals including sheep and goats for various purposes (Figure 1). Figure 1 Applications and aims of genome engineering in sheep and goats. About 40 years ago, a set of basic techniques were applied to sheep embryos with the desire to generate identical twins, multiplets, and chimeras. FRONT BIOENG BIOTECHNOLOL - 2019 - Regulatory Status of Genome-Edited Organisms Under the Japanese Cartagena Act. EFSA 07/08/19 Literature review of baseline information on non‐coding RNA (ncRNA) to support the risk assessment of ncRNA‐based genetically modified plants for food and feed.

BMC VETERINARY RESEARCH 27/11/19 Development of a CRISPR/Cas9 system against ruminant animal brucellosis. Ovine macrophages and bacteria Ovine macrophages were obtained by culturing the mononuclear cell fraction of sheep peripheral blood after ficoll density gradient centrifugation [29].

BMC VETERINARY RESEARCH 27/11/19 Development of a CRISPR/Cas9 system against ruminant animal brucellosis

Brucella melitensis 16 M strain (ATCC 23456) was purchased from Culture Collections Public Health England (Salisbury, United Kingdom). Brucellae were cultured as previously described [30]. Briefly, Brucella master seed was obtained by aerobically culturing purchased bacteria both on Brucella agar (Oxoid, Hampshire, United Kingdom) and Columbia agar sheep blood plates (Oxoid, Hampshire, United Kingdom) for 3 days at 37ο C. Vials containing 1 × 108 CFU/ml B.melitensis in brain heart broth (Oxoid, Hampshire, United Kingdom) with 15% glycerol (Sigma-Aldrich, St. To infect macrophages at particular MOI, 10-fold serial dilutions of freshly cultured bacteria were plated on Columbia agar sheep blood plates and CFUs were determined after 3 days to estimate bacterial concentrations per ml.

Biochem Soc Trans. 2013 Dec;41(6): Streptococcus zooepidemicus and Streptococcus equi evolution: the role of CRISPRs. Streptococcus zooepidemicus and Streptococcus equi evolution: the role of CRISPRs AS Waller, C Robinson - 2013 - portlandpress.com The host-restricted bacterium Streptococcus equi is the causative agent of equine strangles, the most frequently diagnosed infectious disease of horses worldwide.

Biochem Soc Trans. 2013 Dec;41(6): Streptococcus zooepidemicus and Streptococcus equi evolution: the role of CRISPRs.

The disease is characterized by abscessation of the lymph nodes of the head and neck, leading to significant welfare and economic cost. S. equi is believed to have evolved from an ancestral strain of Streptococcus zooepidemicus, an opportunistic pathogen of horses and other animals. Comparison of the genome of S. equi strain 4047 with those of S. zooepidemicus … APHIS USDA 19/04/19 Genome Edited Pennycress Lines Developed with CRISPR. ANNUAL REVIEWS - AVRIL 2019 - CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture. ADVANCED SCIENCE 06/02/20 CRISPR/Cas Systems in Genome Editing: Methodologies and Tools for sgRNA Design, Off‐Target Evaluation, and Strategies to Mitigate Off‐Target Effects. 1 E‐CRISP/Cas Systems Genome editing (GE) tools have modernized the genetics by their potential use in the precise modification of genomic DNA.1 These GE tools include ZFNs (zinc finger nucleases), TALENs (transcription activator‐like effector nucleases), CRISPR (clustered regulatory interspaced short palindromic repeats)/Cas9 (CRISPR‐associated proteins), and related CRISPR/Cas systems.

ADVANCED SCIENCE 06/02/20 CRISPR/Cas Systems in Genome Editing: Methodologies and Tools for sgRNA Design, Off‐Target Evaluation, and Strategies to Mitigate Off‐Target Effects

Among these GE tools, CRISPR/Cas systems are extensively used in comparison with other methods because they are cost‐effective, easy to use, and do not require specialist skills.2 The CRISPR/Cas9 system requires an RNA designing with a short guide sequence (sgRNA) that directs a Cas9 nuclease for cleaving any target sequence.3 Cas9 is a CRISPR RNA‐guided endonuclease that cuts dsDNA targets complementary to the sgRNAs4 and is being exploited for GE in bacteria5 and in eukaryotic cells,6 including animal cells,7 mammalian systems,8, 9 and plants.10, 11.

FRONT. PLANT SCI. 13/02/20 Principles, Applications, and Biosafety of Plant Genome Editing Using CRISPR-Cas9. Introduction The world population is predicted to reach 10 billion by 2050.

FRONT. PLANT SCI. 13/02/20 Principles, Applications, and Biosafety of Plant Genome Editing Using CRISPR-Cas9

While the available farm land and water are being reduced, the global demand for food will increase 25%–70% above current production levels (Hunter et al., 2017). Thus, feeding a rapidly growing population, particularly in the face of climate change, is a big challenge. There is, therefore, an urgent need to improve food production and accelerate sustainable agricultural development. Long time before the field of genetics was established, humans genetically modified plants through breeding and selection. To make plant breeding faster, more predictable, and amendable to a wide range of species, several techniques of plant genetic engineering have been developed. BIORXIV 12/03/20 A genome-scale CRISPR interference guide library enables comprehensive phenotypic profiling in yeast.

Theriogenology. 2016 Jul 1;86(1):160-9. New insights and current tools for genetically engineered (GE) sheep and goats. Genetically engineered sheep and goats represent useful models applied to proof of concepts, large-scale production of novel products or processes, and improvement of animal traits, which is of interest in biomedicine, biopharma, and livestock.

Theriogenology. 2016 Jul 1;86(1):160-9. New insights and current tools for genetically engineered (GE) sheep and goats.

This disruptive biotechnology arose in the 80s by injecting DNA fragments into the pronucleus of zygote-staged embryos. Pronuclear microinjection set the transgenic concept into people's mind but was characterized by inefficient and often frustrating results mostly because of uncontrolled and/or random integration and unpredictable transgene expression. Somatic cell nuclear transfer launched the second wave in the late 90s, solving several weaknesses of the previous technique by making feasible the transfer of a genetically modified and fully characterized cell into an enucleated oocyte, capable of cell reprogramming to generate genetically engineered animals.

NATURE 15/01/20 The kill-switch for CRISPR that could make gene-editing safer. NATURE 19/12/19 Multi-functional genome-wide CRISPR system for high throughput genotype–phenotype mapping. FRONT. GENET. 03/09/19 Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era. PLOS 12/11/19 CRISPR/Cas9 gene editing in the West Nile Virus vector, Culex quinquefasciatus Say. Abstract Culex quinquefasciatus Say is an opportunistic blood feeder with a wide geographic distribution which is also a major vector for a range of diseases of both animals and humans.

CRISPR/Cas technologies have been applied to a wide variety of organisms for both applied and basic research purposes. CRISPR/Cas methods open new possibilities for genetic research in non-model organisms of public health importance. In this work we have adapted microinjection techniques commonly used in other mosquito species to Culex quinquefasciatus, and have shown these to be effective at generating homozygous knock-out mutations of a target gene in one generation.

This is the first description of the kmo gene and mutant phenotype in this species. Citation: Anderson ME, Mavica J, Shackleford L, Flis I, Fochler S, Basu S, et al. (2019) CRISPR/Cas9 gene editing in the West Nile Virus vector, Culex quinquefasciatus Say. Editor: Luciano Andrade Moreira, Fundacao Oswaldo Cruz Instituto Rene Rachou, BRAZIL. BIORXIV 13/11/19 CRISPR screening of porcine sgRNA library identified host factors essential for Japanese encephalitis virus replication. PARLEMENT EUROPEEN - Réponse à question E-002850-19 New initiatives in respect of CRISPR-Cas. EFSA 07/08/19 Literature review of baseline information on non‐coding RNA (ncRNA) to support the risk assessment of ncRNA‐based genetically modified plants for food and feed. Biotechnology Research and Innovation Available online 26 July 2019 An overview of regulatory approaches to genome editing in agriculture.

Background Genome editing refers to techniques, in which specialised enzymes that have been modified can insert, replace, or remove DNA from a genome with a high degree of specificity; the techniques represent the latest innovation in the toolbox of genetic engineering/modification (GE/GM) methods. Especially the most discussed genome editing system known as CRISPR/Cas9 (i.e. “Clustered Regularly Interspaced Short Palindromic Repeats”, using the CRISPR-associated protein 9) has received wide-spread application, because it enables the development of easily deployable low-cost tools for innovation in biomedicine, agriculture, industrial biotechnology and other sectors relating to the bioeconomy. Table 1. Four types of genome editing need to be distinguished, especially with regard to potential discrepancies in the regulatory approaches to the techniques and/or their products.

Adapted from Ricroch (2019) and Friedrichs et al. (2019). SCIENTIFIC REPORTS 17/05/19 CRISPR/Cas9-mediated ablation of elovl2 in Atlantic salmon (Salmo salar L.) inhibits elongation of polyunsaturated fatty acids and induces Srebp-1 and target genes. Biochem Soc Trans. 2013 Dec;41(6): Streptococcus zooepidemicus and Streptococcus equi evolution: the role of CRISPRs. NATURE 20/03/19 Proposed U.S. regulation of gene-edited food animals is not fit for purpose. TRENDS IN BIOTECHNOLOGY 14/01/19 CRISPR/Cas Systems towards Next-Generation Biosensing. THE SCIENTIST 02/04/18 USDA Will Not Regulate CRISPR-Edited Crops. PLOS 13/02/18 CRISPR/Cas9 in Leishmania mexicana: A case study of LmxBTN1. PENNSTATE 08/08/18 New technology improves CRISPR-Cas9 gene editing in mosquitoes, other species. A technology designed to improve CRISPR-Cas9 gene editing in mosquitoes and other arthropods succeeds with a high degree of efficiency, while eliminating the need for difficult microinjection of genetic material, according to researchers.

These results could pave the way for scientists examining a wide range of arthropods -- and even some vertebrates -- to more easily manipulate gene expression for fundamental research and practical applications such as control of vector-borne diseases like Zika virus and malaria, elimination of agricultural insect pests, and potentially gene therapy for human and animal health. CRISPR -- Clustered Regularly Interspaced Short Palindromic Repeats -- is a relatively new and revolutionary way to modify an organism's genome by precisely delivering a DNA-cutting enzyme, Cas9, to a targeted region of DNA.

The resulting mutation can delete or replace specific DNA pieces, thereby promoting or disabling certain traits. NUCLEIC ACIDS RESEARCH 22/06/18 A functional type II-A CRISPR–Cas system from Listeria enables efficient genome editing of large non-integrating bacteriophage. Skip to Main Content Sign In Register Close. JOURNAL OF ARTHROPOD-BORNE DISEASES 30/12/17 Curbing Malaria: A New Hope through Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Technology. Gonzaga-Jauregui C, Lupski JR, Gibbs RA (2012) Human genome se¬quenc-ing in health and disease.

Annu Rev Med. 63: 35–61. Grissa I, Vergnaud G, Pourcel C (2007) The CRISPRdb database and tools to display CRISPRs and to generate dic¬tionaries of spacers and repeats. BMC Bioinformatics. 8: 172. Wiedenheft B, Sternberg SH, Doudna JA (2012) RNA-guided genetic silencing systems in bacteria and archaea. Na¬ture. 482(7385): 331–338. Bhaya D, Davison M, Barrangou R (2011) CRISPR-Cas Systems in Bacteria and Archaea: Versatile Small RNAs for Adap¬tive Defense and Regulation.

INT. J. MOL. SCI. 05/04/18 Applications of CRISPR/Cas System to Bacterial Metabolic Engineering. 3.1. FROMSOILFORLIFE 14/06/18 CRISPR CAS 9 is the future of Sustainable Agriculture? The urgent needs Crops provide food, feed and fibre for humankind. GENOME MEDICINE - 2018 - Pathogen detection in the CRISPR–Cas era.

DISCOVERMAGAZINE 24/10/18 Scientists Use CRISPR To Protect Pigs Against Deadly Virus. Scientists used the gene editing technology CRISPR to stop these pigs from getting a deadly flu. BIORXIV 15/05/18 CRISPR/Cas9 gene editing to make conditional mutants of the human malaria parasite Plasmodium falciparum. BIORXIV 02/05/18 CRISPR-Cas blocks antibiotic resistance plasmid transfer between Enterococcus faecalis strains in the gastrointestinal tract. NATURE 31/01/18 The future of CRISPR technologies in agriculture.

BIOTECHNIQUES - NOV 2016 - CRISPR: MODIFYING THE LIFE SCIENCE LANDSCAPE. BUSINESS INSIDER 27/03/18 Monsanto is on the verge of producing the first fruit made with a blockbuster gene-editing tool that could revolutionize agriculture. PENNSTATE 08/08/18 New technology improves CRISPR-Cas9 gene editing in mosquitoes, other species. JOURNAL OF ARTHROPOD-BORNE DISEASES 30/12/17 Curbing Malaria: A New Hope through Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Technology. DISCOVERMAGAZINE 24/10/18 Scientists Use CRISPR To Protect Pigs Against Deadly Virus. PLOS 13/02/18 CRISPR/Cas9 in Leishmania mexicana: A case study of LmxBTN1.

Abstract Leishmania parasites cause human cutaneous, mucocutaneous and visceral leishmaniasis. Several studies proposed involvement of certain genes in infectivity of these parasites based on differential mRNA expression data. Due to unusual gene expression mechanism, functions of such genes must be further validated experimentally. Here, we investigated a role of one of the putative virulence factors, LmxM.22.0010-encoded BTN1 (a protein involved in Batten disease in humans), in L. mexicana infectivity.

Due to the incredible plasticity of the L. mexicana genome, we failed to obtain a complete knock-out of LmxM.22.0010 using conventional recombination-based approach even after ablating four alleles of this gene. Citation: Ishemgulova A, Hlaváčová J, Majerová K, Butenko A, Lukeš J, Votýpka J, et al. (2018) CRISPR/Cas9 in Leishmania mexicana: A case study of LmxBTN1. Editor: Andrew Paul Jackson, University of Liverpool, UNITED KINGDOM Copyright: © 2018 Ishemgulova et al. Introduction.

FRONT. PLANT SCI. 06/08/18 The enhancement of plant disease resistance using CRISPR/Cas9 technology. 1Università Cattolica del Sacro Cuore, Italy 2Department of Agricultural and Environmental Sciences, Università degli Studi di Milano, Italy 3Laboratoire Reproduction et Développement des Plantes, Université de Lyon, France Genome editing technologies have progressed rapidly and become one of the most important genetic tools in the implementation of pathogen resistance in plants. NUCLEIC ACIDS RESEARCH 22/06/18 A functional type II-A CRISPR–Cas system from Listeria enables efficient genome editing of large non-integrating bacteriophage.

BERKELEY_EDU - From Genes to Global Solutions CNR faculty lead wide-ranging research initiatives at the Innovative Genomics Institute - Revolutionizing agriculture in the developing world (CRISPR) Zilberman is a little more blunt, focusing on the risks of not using CRISPR. THE SCIENTIST 02/04/18 USDA Will Not Regulate CRISPR-Edited Crops. Restrictions will remain on transgenic plants, which contain artificially inserted genes from other species. NATIONAL ACADEMY OF SCIENCES (NAS) - 2018 NAS prize in food and agriculture sciences. (concerne CRISPR) ZMESCIENCE 05/09/17 CRISPR used for first time to change flower color in Japanese ornamental plant. With the help of the mighty CRISPR gene editing tool, scientists changed the flower color of a traditional Japanese garden plant from violet to white. The alteration was so precise only a single gene disruption was enough to introduce this effect.

UNIVERSITY OF GEORGIA 06/02/17 Researchers receive $2.78 million NIH grant to explore, exploit bacterial immune systems. THE SCIENTIST 10/08/17 Jumping Genes Inactivated with CRISPR in Pigs. Sci Rep. 2018 Jan 23;8(1):1 Efficient inhibition of African swine fever virus replication by CRISPR/Cas9 targeting of the viral p30 gene (CP204L). Plant Biotechnol J. 2017 Sep 14. CRISPR/Cas9-mediated efficient targeted mutagenesis in grape in the first generation. PEERJ 09/05/16 Genome editing weds CRISPR: what is in it for phytoremediation? NATURE SCIENTIFIC REPORTS 01/11/17 Analysis of the CRISPR-Cas system in bacteriophages active on epidemic strains of Vibrio cholerae in Bangladesh. NATURE SCIENTIFIC REPORT 16/02/18 CRISPR-Cas9, a tool to efficiently increase the development of recombinant African swine fever viruses. NATURE 16/05/17 Geneticists enlist engineered virus and CRISPR to battle citrus disease. MEDIUM 12/04/18 Will You Eat CRISPR Produce? Gene editing will make plants hardier and healthier. Hopefully people won’t freak out.

FRONT PHYSIOL 06/09/17 Progress and Prospects of CRISPR/Cas Systems in Insects and Other Arthropods. ECDC 03/05/17 ECDC assesses risk of ‘do-it-yourself’ CRISPR gene engineering kit contaminated with pathogenic bacteria. CORNELL ALLIANCE FOR SCIENCE - AVRIL 2018 - Bill Gates: Gene editing can help humanity. Channel SRUK-CERU VIA YOUTUBE 28/02/18 5th Anniversary Seminar in Edinburgh: CRISPR - Prof. Bruce Whitelaw, Roslin Institute (08-04-2017) BUSINESS INSIDER 27/03/18 Monsanto is on the verge of producing the first fruit made with a blockbuster gene-editing tool that could revolutionize agriculture. BUSINESS INSIDER 12/04/18 A controversial technology could save us from starvation — if we let it (CRISPR)

BUSINESS INSIDER 27/03/18 A new Monsanto-backed company is on the verge of producing the first fruit made with a blockbuster gene-editing tool that could revolutionize agriculture. BMC Res Notes. 2017 Nov 7;10(1):578. Phage typing or CRISPR typing for epidemiological surveillance of Salmonella Typhimurium? BFR 06/03/18 FAQ on Genome Editing and CRISPR/Cas9. Acta Pharmaceutica Sinica B Volume 7, Issue 3, May 2017, Application of CRISPR/Cas9 in plant biology. GENETIC LITERACY PROJECT 14/01/16 How should Europe regulate CRISPR, new breeding technologies (NBTs)? Front Plant Sci. 2017; 8: 1418. New Biotechnological Tools for the Genetic Improvement of Major Woody Fruit Species. NATURE SCIENTIFIC REPORT 16/02/18 CRISPR-Cas9, a tool to efficiently increase the development of recombinant African swine fever viruses.

BUSINESS INSIDER 12/04/18 A controversial technology could save us from starvation — if we let it (CRISPR) BUSINESS INSIDER 27/03/18 Monsanto is on the verge of producing the first fruit made with a blockbuster gene-editing tool that could revolutionize agriculture. NATIONAL REVIEW 19/03/18 CRISPR Will Make GMOs Ubiquitous. SCIENCE DAILY 08/03/18 Gene knockout using new CRISPR tool makes mosquitoes highly resistant to malaria parasite Study highlights the potential of mosquito gene-modification to combat malaria. Science. 2017 Sep 22;357(6357) Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9. VIRUSES 13/02/18 The Application of NHEJ-CRISPR/Cas9 and Cre-Lox System in the Generation of Bivalent Duck Enteritis Virus Vaccine against Avian Influenza Virus. Viruses. 2018 Jan 30;10(2). CRISPR-Cas9 Genetic Analysis of Virus-Host Interactions. GENETIC LITERACY PROJECT 24/01/18 Genetic engineering, CRISPR and food: What the 'revolution' will bring in the near future.

FOODINSIGHT 29/01/18 What does crispr mean for our food system? GENETIC LITERACY PROJECT 30/01/18 CRISPR gene-edited food in Europe? Questions remain on crop breeding regulations. Sci Rep. 2018 Jan 23;8(1):1 Efficient inhibition of African swine fever virus replication by CRISPR/Cas9 targeting of the viral p30 gene (CP204L). AStA Uni Bielefeld VIA YOUTUBE 24/11/16 CRISPR Panel Debate Bielefeld University.

EMERGING TOPICS IN LIFE SCIENCES 10/11/17 Use of CRISPR systems in plant genome editing: toward new opportunities in agriculture. BMC Res Notes. 2017 Nov 7;10(1):578. Phage typing or CRISPR typing for epidemiological surveillance of Salmonella Typhimurium? NATURE SCIENTIFIC REPORTS 01/11/17 Analysis of the CRISPR-Cas system in bacteriophages active on epidemic strains of Vibrio cholerae in Bangladesh. FRONTIERS IN PLANT SCIENCE 22/09/17 CRISPR/Cas9 Mediated Genome Engineering for Improvement of Horticultural Crops.

PLOS 18/05/17 CRISPR/Cas9-mediated targeted mutagenesis in grape. Plant Biotechnol J. 2017 Sep 14. CRISPR/Cas9-mediated efficient targeted mutagenesis in grape in the first generation. ZMESCIENCE 05/09/17 CRISPR used for first time to change flower color in Japanese ornamental plant. WASHINGTON POST 10/08/17 Scientists create the first mutant ants. PHYSORG 10/08/17 Researchers use CRISPR to manipulate social behavior in ants. THE SCIENTIST 10/08/17 Jumping Genes Inactivated with CRISPR in Pigs The study could pave the way for transplanting porcine organs to humans without the risk of reigniting endogenous retroviruses. NEWSWEEK 05/08/17 Gene Editing Could Stop Cancer, Diabetes and Bioterrorism: An Interview With CRISPR Scientist Jennifer Doudna.

Acta Pharmaceutica Sinica B Volume 7, Issue 3, May 2017, Application of CRISPR/Cas9 in plant biology. GENOME BIOLOGY 14/06/17 Unexpected consequences: exon skipping caused by CRISPR-generated mutations.