Current Opinion in Virology Volume 44, October 2020, Highly efficient vaccines for Bluetongue virus and a related Orbivirus based on reverse genetics. Introduction Virus reverse genetics (RG) techniques are based on the ability to rescue viable virus containing designed genetic changes.
The role of those changes in virus replication or pathogenicity can then be assessed. The technique relies on the transfection of cells with nucleic acids encoding the viral genome, or mutants thereof, and was developed first for DNA viruses and subsequently for positive-sense RNA viruses. Gradually reverse genetics technology has been developed for all the major virus families.
You can change your cookie settings at any time. <a href=" Find out more</a> Skip to Main Content Search Close Advanced Search Search Menu Article Navigation Prospective Study of Epizootic Hemorrhagic Disease Virus and Bluetongue Virus Transmission in Captive Ruminants Michael E Becker Department of Entomology, Louisiana State University Agricultural Center, Agricultural Experiment Station , Life Sciences, Baton Rouge, LA Corresponding author, e-mail: email@example.com Search for other works by this author on: Oxford Academic PubMed Google Scholar Michael E Becker, Jonathan Roberts Louisiana Department of Agriculture and Forestry , LSU Union Square, Baton Rouge, LA Oxford Academic PubMed Google Scholar Jonathan Roberts, Megan E Schroeder Texas A&M Veterinary Medical Diagnostic Laboratory , College Station, TX Oxford Academic PubMed Google Scholar Megan E Schroeder, Glen Gentry.
PARASITES & VECTORS 11/10/19 Vector competence is strongly affected by a small deletion or point mutations in bluetongue virus. Rescue of BTV11 To study the role of viral proteins in BTV replication in midges, we first regenerated BTV11 by reverse genetics (rgBTV11).
As expected, rgBTV11 efficiently replicated in vitro in BSR and KC cells (Fig. 1). We also showed that rgBTV11 replicates in competent midges like wild type BTV11 (wtBTV11) as previously shown . Thus, rgBTV11 is indistinguishable from wtBTV11 and an attractive virus backbone to study the role of viral proteins in differential virus replication in vitro and in vivo. BIORXIV 08/09/19 Understanding the effect of temperature on Bluetongue disease risk in livestock. Research in Veterinary Science Volume 115, December 2017, Bluetongue outbreaks: Looking for effective control strategies against Culicoides vectors. BMC Genomics. 2018 Aug 22;19(1):624. The genome of the biting midge Culicoides sonorensis and gene expression analyses of vector competence for bluetongue virus. MEDICAL AND VETERINARY ENTOMOLOGY 27/07/17 First molecular identification of the vertebrate hosts of Culicoides imicola in Europe and a review of its blood‐feeding patterns worldwide: implications for the transmission of bluetongue disease and African ho.
CABI 30/03/18 BLUETONGUE. Datasheet Don't need the entire report?
Generate a print friendly version containing only the sections you need. Generate report Pictures Top of page Identity Preferred Scientific Name. Vet. Ital., 40 (4), 456-461 2004 A potential overwintering mechanism for bluetongue virus – recent findings. Rev. Salud Anim. Vol. 38 No. 1 (ene.-abr. 2016): 52-59 Bluetongue virus: a known virus, a current threat. UNIVERSITY OF PRETORIA 25/06/16 Thèse en ligne : Reassortment of bluetongue virus vaccine serotypes in cattle. CRITICAL REVIEWS IN MICROBIOLOGY 01/11/16 Current and next-generation bluetongue vaccines: Requirements, strategies, and prospects for different field situations. FNU 24/10/17 Bluetongue: What you need to know. What is bluetongue? Bluetongue disease is caused by a virus transmitted by biting midges, which are most active between May and October. Bluetongue virus can infect all ruminants (e.g. sheep, cattle, goats and deer) and camelids (e.g. llama and alpaca). Sheep are most severely affected by the disease.
Cattle, although infected more frequently than sheep, do not always show signs of the disease. VETERINARY WORLD 04/10/17 Analysis of spatial dynamic of epizootic process of bluetongue and its risk factors. Research (Published online: 04-10-2017) 4.
Analysis of spatial dynamic of epizootic process of bluetongue and its risk factors Fayssal Bouchemla, Olga Mikhailovna Popova and Valerey Alexandrovich Agoltsov. Veterinary Microbiology Available online 17 September 2017 Bluetongue virus infection in naïve cattle: identification of circulating serotypes and associated Culicoides biting midge species in Trinidad. <div pearltreesdevid="PTD135" role="alert" class="alert-message-container"><div pearltreesdevid="PTD136" aria-hidden="true" class="alert-message-body"><span pearltreesdevid="PTD137" style="display: inline-block;" class="Icon IconAlert"><svg pearltreesDevId="PTD138" style="width: 100%; height: 100%;" width="24" height="24" focusable="false" tabindex="-1" fill="currentColor"><path pearltreesDevId="PTD139" fill="#f80" d="M11.84 4.63c-.77.05-1.42.6-1.74 1.27-1.95 3.38-3.9 6.75-5.85 10.13-.48.83-.24 1.99.53 18.104.22.168 1.66.36 2.5.41 3.63 0 7.27.01 10.9-.01 1.13-.07 2.04-1.28 1.76-2.39-.1-.58-.56-1.02-.81-1.55-1.85-3.21-3.69-6.43-5.55-9.64-.42-.52-1.06-.83-1.74-.79z"></path><path pearltreesDevId="PTD140" d="M11 8h2v5h-2zM11 14h2v2h-2z"></path></svg></span><!
Intermountain West have prompted questions about the economic benefits and costs of vaccinating individual flocks against bluetongue disease. We estimate the cost of a bluetongue outbreak on a representative rangeland sheep operation in the Big Horn Basin of the state of Wyoming using enterprise budgets and stochastic simulation. The latter accounts for variability in disease severity and lamb price, as well as uncertainty about when an outbreak will occur. We then estimate the cost of purchasing and administering a bluetongue vaccine. Digitalgreenorg VIA YOUTUBE 09/10/14 Blue tongue disease treatment in sheep using Aloe vera.
Bluetongue and Epizootic Hemorrhagic Diseases Part1 Jim MacLachlian. NATURE SCIENTIFIC REPORTS 08/02/16 Using shared needles for subcutaneous inoculation can transmit bluetongue virus mechanically between ruminant hosts. Global Veterinaria 17 (1): 63-77, 2016 Diagnosis, Prevention and Control of Blue Tongue in Sheep: Review. VIROLOGY JOURNAL 02/07/16 Requirements and comparative analysis of reverse genetics for bluetongue virus (BTV) and African horse sickness virus (AHSV) Bluetongue (BT), African horse sickness (AHS), and epizootic haemorrhagic disease (EHD) are OIE listed arthropod borne animal diseases caused by the viruses in the genus Orbivirus, family Reoviridae.
These viruses are spread by specific species of Culicoides biting midges. Outbreaks and geographic expansion of affected areas are associated to various factors, including climate change [1, 2], and the presence of competent biting Culicoides midges . Culicoides species in countries with a moderate climate are competent insect vectors for BTV [4, 5, 6].
This implies that countries historically free of disease are probably at risk for BT and other midge borne diseases . The prototype bluetongue virus (BTV) and African horse sickness virus (AHSV) are the best studied orbiviruses, reviewed in . For a long time, research on orbiviruses has been hampered by the lack of reverse genetics systems. RG has also been used to develop very promising novel vaccines for orbiviruses. Parasites & Vectors 17/09/15 Bluetongue virus infection creates light averse Culicoides vectors and serious errors in transmission risk estimates. Because BTV is not transovarially transmitted  only previously blood-fed (parous) females were tested for BTV.
Of 674 total pools (representing about 13,000 parous female midges), 212 were from CO2 traps, 145 were from UV traps, and 317 were from CO2 + UV traps. Of the tested pools, 126 (18.7 %) were positive for BTV (Ct < 31). Pool sizes (parous insects per pool) did not differ significantly for the three trap types (p = 0.421). When the data from all three dairies were combined, there was an overall highly significant difference between the traps (×2 = 76.52, df = 2, p < 0.001) (Table 2).
Despite comprising similar proportions of the total pools, CO2 traps and UV traps accounted for vastly different proportions of the positive pools. VETERINARIA ITALIANA ISSUES ONLINE 2015 - Volume 51 (4), October-December The changing global face of Bluetongue: from the beginning. VETERINARIA ITALIANA ISSUES ONLINE 2015 - Volume 51 (4), October-December Bluetongue: a disease that does not speak 'one tongue' only.
It is now 1 year since the IV International Conference on Bluetongue and Related Orbiviruses closed its doors.
This is the first of 2 issues of Veterinaria Italiana dedicated to this international event, collecting selected papers presented at the conference. A second issue will be published at the beginning of next year. Thinking of the Rome conference, I am sure I am not wrong when I say that it was really a great meeting, whose success is well represented in Ford's words: "coming together is a beginning; keeping together is progress; working together is success" (Henry Ford).
Exp Appl Acarol. 2010 Oct; 52(2): 183–192. Potential role of ticks as vectors of bluetongue virus. VET ITAL - 2004 - Environmental effects on vector competence and virogenesis of bluetongue virus in Culicoides: interpreting laboratory data in a field context. VET. ITAL - 2004 - The history of bluetongue and a current global overview.
Braz. arch. biol. technol. vol.56 no.6 Curitiba Nov./Dec. 2013 Epub Oct 25, 2013 Evaluation of different adjuvants formulations for bluetongue vaccine. Evaluation of different adjuvants formulations for bluetongue vaccine Ludmila Branco MacedoI; Zélia Inês Portela LobatoII; Sílvia Ligório FialhoIII; Aline de Marco ViottII; Roberto Maurício Carvalho GuedesII; Armando Silva-CunhaI,* IFaculdade de Farmacia; Universidade Federal de Minas Gerais; Belo Horizonte - MG - Brasil IIEscola de Veterinaria; Universidade Federal de Minas Gerais, Belo Horizonte - MG - Brasil IIIDivisão de Desenvolvimento Farmacotécnico e Biotecnológico; Fundação Ezequiel Dias; Belo Horizonte - MG - Brasil This study investigated the adjuvant potential of W/O/W multiple emulsions and microemulsions, comparing them with traditional aluminum hydroxide and oil-in-water emulsion adjuvants against bluetongue vaccine (BTV).
Local inflammatory reactions were assessed in rabbits by measuring the temperature of the animals and the skin thickness at the site of application. Advances in Animal and Veterinary Sciences - 2013 - Diversity among Topotypes of Bluetongue Virus Serotype 9 as Revealed by Whole Genome Sequence Analysis. Surveillance network for bluetongue in the Eastern and Balkanic Regions EAST BTNET - Bibliographie 1998 à 2006. 40 (4), October-December Bluetongue proceedings, Part II. VETERINARIA ITALIANA40 (3), July-September Bluetongue proceedings, Part I. VETERINARY WORLD - OCT 2011 - Au sommaire notamment: Studies of the Antigenic relationships between Bluetongue virus serotypes 2.
Open Access, Research 2. Studies of the antigenic relationships between bluetongue virus serotypes 2, 9 & 15 isolated in Andhra Pradesh, India - Deepthi Balam, Sreenivasulu Daggupati, Himaja Maddireddy Vet World. 2011; 4(10): 444-448 Abstract l PDF. Sci Rep. 2012; 2: 319. Modelling bluetongue virus transmission between farms using animal and vector movements. Journal of Life Sciences - JANV 2013 - Bluetongue: A Hypothesis of Control Strategy through Decrease of Culicoides and Their Ass. Genome Announc. 2013 Jul-Aug; 1(4): e00403-13. Full-Genome Sequence of Bluetongue Virus Serotype 1 (BTV-1) Strain Y863, the Firs.
INSTITUTE FOR ANIMAL HEALTH PIRBRIGHT 05/05/10 Présentation : Bluetongue on the wind: Long distance dispersal of Culicoides vect. VIROLOGY JOURNAL 11/06/15 Identification of four novel group-specific bluetongue virus NS3 protein B-cell epitopes. With the trends of global warming and the expansion of activities of Culicoides midges, BT disease has raised global attention with its extensively spreading. Up to date, 27 distinct BTV serotypes make diagnose and prevention of BTV more difficult and complicated. How to make group-specific diagnose of BTV quickly and efficiently is crucial. We focused on NS3 protein of BTV to develop better group-specific diagnose and research reagents. NS3 protein may represent a target for BTV group-specific diagnose because NS3 protein is highly conserved among different BTV serotypes. NS3 has been shown to possess protein-protein interaction domain that facilitate interactions with various proteins in infected cells, including cellular proteins p11, ESCRT-I protein Tsg101 and the BTV VP2 protein, involving in trafficking and release of BTV [17, 19, 25].
Tamilnadu J. Veterinary & Animal Sciences 8 (2) 101-103, March - April, 2012 - DEVELOPMENT OF MULTIPLEX RT-PCR FOR THE DETERMINATION OF BLUETONGUE VIRUS SEROTYPES.
Références VETERINARY RESEARCH. Advances in Animal and Veterinary Sciences - SEPT 2015 - Seroprevalence of Bluetongue in Free-range Mithuns (Bos frontalis) Global Veterinaria 14 (6): 800-804, 2015 Observations on the Epidemiology of Bluetongue Disease.