background preloader

OGM ET CANNE A SUCRE

Facebook Twitter

TECHNOLOGY NETWORKS 13/07/21 First Breeding of Sugar Cane Using CRISPR/Cas9. Sugarcane is one of the most productive plants on Earth, providing 80 percent of the sugar and 30 percent of the bioethanol produced worldwide.

TECHNOLOGY NETWORKS 13/07/21 First Breeding of Sugar Cane Using CRISPR/Cas9

Its size and efficient use of water and light give it tremendous potential for the production of renewable value-added bioproducts and biofuels. But the highly complex sugarcane genome poses challenges for conventional breeding, requiring more than a decade of trials for the development of an improved cultivar. Two recently published innovations by University of Florida researchers at the Department of Energy's Center for Advanced Bioenergy and Bioproducts Innovation (CABBI) demonstrated the first successful precision breeding of sugarcane by using CRISPR/Cas9 genome editing -- a far more targeted and efficient way to develop new varieties.

CRISPR/Cas9 allows scientists to introduce precision changes in almost any gene and, depending on the selected approach, to turn the gene off or replace it with a superior version. SUGARCANE BIOFUELS 29/06/19 Genetically Modified Sugarcane for Biofuels Production: Status and Perspectives of Conventional Transgenic Approaches, RNA Interference, and Genome Editing for Improving Sugarcane for Biofuels. PLANTS 06/02/20 Overexpression of Sucrose Phosphate Synthase Enhanced Sucrose Content and Biomass Production in Transgenic Sugarcane. All articles published by MDPI are made immediately available worldwide under an open access license.

PLANTS 06/02/20 Overexpression of Sucrose Phosphate Synthase Enhanced Sucrose Content and Biomass Production in Transgenic Sugarcane

No special permission is required to reuse all or part of the article published by MDPI, including figures and tables. For articles published under an open access Creative Common CC BY license, any part of the article may be reused without permission provided that the original article is clearly cited. Feature Papers represent the most advanced research with significant potential for high impact in the field. Feature Papers are submitted upon individual invitation or recommendation by the scientific editors and undergo peer review prior to publication. The Feature Paper can be either an original research article, a substantial novel research study that often involves several techniques or approaches, or a comprehensive review paper with concise and precise updates on the latest progress in the field that systematically reviews the most exciting advances in scientific literature.

MOLECULAR BIOTECHNOLOGY 24/04/21 Genetic Engineering Approaches for Enhanced Insect Pest Resistance in Sugarcane. 1.FAOSTAT. (2018).

MOLECULAR BIOTECHNOLOGY 24/04/21 Genetic Engineering Approaches for Enhanced Insect Pest Resistance in Sugarcane

Sugar cane. (Food and Agriculture Organization of the United Nations) Major Food and Agricultural Commodities and Producers (2018).3.Hoang, N. V., Furtado, A., Botha, F. C., Simmons, B. A., & Henry, R. MOLECULAR BIOLOGY REPORTS 10/04/21 Induction of resistance to sugarcane mosaic virus by RNA interference targeting coat protein gene silencing in transgenic sugarcane. ISAAA 14/07/21 CABBI Reports First Successful Precision Breeding of Sugarcane Using CRISPR-Cas9 Genome Editing. July 14, 2021 Ayman Eid, CABBI Postdoctoral Research Associate at the University of Florida, displays gene-edited sugarcane with reduced chlorophyll content.

ISAAA 14/07/21 CABBI Reports First Successful Precision Breeding of Sugarcane Using CRISPR-Cas9 Genome Editing

Photo Source: Rajesh Yarra, UF/IFAS Agronomy. University of Florida researchers at the U.S. Department of Energy's Center for Advanced Bioenergy and Bioproducts Innovation (CABBI) reveal the first successful precision breeding of sugarcane by using CRISPR-Cas9 genome editing, published in two reports in Frontiers in Genome Editing. In the first report, researchers demonstrated the ability to turn off variable numbers of copies of the magnesium chelatase gene, a key enzyme for chlorophyll biosynthesis in sugarcane, producing rapidly identifiable plants with light green to yellow leaves. For more details, read the article on the CABBI website. You might also like: The Crop Biotech Update is a weekly newsletter of ISAAA, a non-for-profit organization.

INTECH 04/05/21 Sugarcane as Future Bioenergy Crop: Potential Genetic and Genomic Approaches. 1.

INTECH 04/05/21 Sugarcane as Future Bioenergy Crop: Potential Genetic and Genomic Approaches

Introduction Increasing energy demands for erratically increasing population, urbanization, industrialization and environmental concerns related to fossil fuels have inclined researchers to explore alternative resources of energy. Compared with the current global energy consumption of 400 exajoule, an increase of 200 exajoule is expected by 2025 [1]. Biofuels have gained much importance due to the depleting fossil fuel resources and the over-accumulation of CO2 and other greenhouse gases in the environment.

Biofuels can play a part to achieve targets to replace fossil fuels to reduce carbon dioxide released into the atmosphere and to attain environmental and economic sustainability. Sugarcane, being a C4 plant is photosynthetically more competent to produce higher content of dry mass. Advertisement La enfermedad de las encías y la salud cardíaca Un estudio nuevo halló que la enfermedad de las encías se vincula a un incremento en el riesgo de presión arterial alta. Gene Volume 753, 30 August 2020, Assessing genetic diversity and population structure of sugarcane cultivars, progenitor species and genera using microsatellite (SSR) markers.

Highlights Genetic diversity assessed among 139 lines of sugarcane and related grasses using SSRs.

Gene Volume 753, 30 August 2020, Assessing genetic diversity and population structure of sugarcane cultivars, progenitor species and genera using microsatellite (SSR) markers

Genetic diversity ranged from 0.43 to 1.00 with an average of 0.740 among the grass accessions. Genetic similarity-based clustering analysis distinguished all the Saccharum and related grasses. Structure analysis indicated that entire population can be grouped into 3 subgroups. Useful for selection of suitable parents, association mapping, and trait specific breeding. Abstract Sugarcane is one among the most important commercial crops used to produce sugar, ethanol, and other byproducts, which significantly contributes in the GDP of India and many other countries around the world. Sugar derived from genetically modified sugarcane. Sugar is an ancient food and currently one of the most used ingredients in human nutrition and in the food industry.

Sugar derived from genetically modified sugarcane

Sugar is used mainly both for its sweetener and energy potential and for offering other desirable characteristics such as texture, body, stability, and volume to foods (Fani, 2011Fani, M. (2011). A evolução do açúcar. Aditivos & Ingredientes, 82, 30-40. Retrieved from , 2014Fani, M. (2014). As múltiplas aplicações do açúcar. The history of sugar use by human nutrition dates to ancient times. Food and Chemical Toxicology Volume 130, August 2019, Risk assessment of genetically modified sugarcane expressing AVP1 gene. Highlights GM AVP1 sugarcane has been developed indigenously which confers drought tolerance.

Food and Chemical Toxicology Volume 130, August 2019, Risk assessment of genetically modified sugarcane expressing AVP1 gene

Toxicological evaluations of GM AVP1 sugarcane was conducted through animal feeding trial leading to biochemical and haematological examinations. FAPESP_BR 24/02/21 Startup creates transgenic sugarcane with enhanced pest resistance. With FAPESP’s support, PangeiaBiotech develops genetically modified varieties of sugarcane that are protected against attacking insects and glyphosate-tolerant (photo: Léo Ramos / Pesquisa FAPESP) February 24, 2021 By Suzel Tunes* | FAPESP Innovative R&D – The sugarcane borer Diatraea saccharalis is the worst threat to this crop in Brazil, causing annual losses estimated at about a billion dollars.

FAPESP_BR 24/02/21 Startup creates transgenic sugarcane with enhanced pest resistance

The moth’s larvae are only about 20 mm long but can ruin a field of sugarcane by tunneling through the stems. To combat it, PangeiaBiotech uses even smaller organisms and genetic engineering tools. The startup based in Campinas, in the state of São Paulo, develops genetically modified (GM) sugarcane varieties that associate expression of two bioinsecticidal proteins from the bacterium Bacillus thuringiensis (Bt) with a gene from another microorganism, Agrobacterium sp., that increases the plant’s tolerance of the herbicide glyphosate.

The scientific rationale has to do with complexity. FAPESP - PESQUISA - DEC 2020 - New transgenic sugarcane. The biggest threat to sugarcane cultivation in Brazil—responsible for an estimated R$5 billion in losses each harvest—is a pest insect slightly more than 20 millimeters long, the larval stage of the Diatraea saccharalis moth, widely known as the sugarcane borer.

FAPESP - PESQUISA - DEC 2020 - New transgenic sugarcane

Now São Paulo company PangeiaBiotech is using even smaller organisms together with genetic engineering tools to combat it. The startup develops varieties of transgenic sugarcane that combine the expression of two proteins from the bacterium Bacillus thuringiensis (Bt) that act as bioinsecticides, with one gene extracted from another microorganism, Agrobacterium sp., which delivers greater tolerance to the herbicide glyphosate.

Genes from the Bt bacteria have been used in transgenic processes aimed at biological pest control for over two decades, in a variety of plants. Crop Breeding and Applied Biotechnology - 2020 - Genetic parameters and selection of sugarcane for borer resistance in the advanced selection stage.

Canne à sucre transgénique par continents

Transgenic Research Volume 20, Number 4, 759-772, 2010 Transgenic sugarcane plants expressing high levels of modified cry1Ac provide effective control against stem borers in field trials. Adang MJ (1991) Bacillus thuringiensis insecticidal crystal proteins. In: Maramorosch K (ed) Gene structure, action and utilization. CRC Press, Boca RatonArencibia A, Molina PR, Delariva G, Selmanhousein G (1995) Production of transgenic sugarcane (Saccharum officinarum L) plants by intact cell electroporation. Plant Cell Rep 14:305–309Article CAS Google Scholar Arencibia A, Vazquez RI, PrietoD TellezP, Carmona ER, Coego A, Hernandez L, DelaRiva GA, SelmanHousein G (1997) Transgenic sugarcane plants resistant to stem borer attack. Mol Breed 3:247–255Article Google Scholar Arencibia AD, Carmona ER, Cornide MT, Castiglione S, O’Relly J, Chinea A, Oramas P, Sala F (1999) Somaclonal variation in insect-resistant transgenic sugarcane (Saccharum hybrid) plants produced by cell electroporation.

SCIENTIFIC REPORTS 09/05/14 Establishment and application of a loop-mediated isothermal amplification (LAMP) system for detection of cry1Ac transgenic sugarcane. Plant materials and genomic DNA Two non-transgenic sugarcane (Saccharum spp.) cultivars, FN95-1702 and ROC22 and seventeen putative cry1Ac transgenic sugarcane clones were used as testing materials. Among them, six clones of 19a-1, 19a-3, 19a-5, 19b-4, 20i-2 and 20i-4, were derived from the variety FN95-1702 and seven clones of 16k-1, 16k-3, 16k-5, 16d-1, 16d-2, 16d-4 and 16d-6, were derived from the variety ROC22. A-2 and A-5 were derived from GT 94-119 and the remaining two, B-2 and B-5, were derived from GT96-44. All the above plant materials are provided by the Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, China. FRONT. PLANT SCI. 08/03/16 Detection of Bar Transgenic Sugarcane with a Rapid and Visual Loop-Mediated Isothermal Amplification Assay. Introduction Sugarcane (Saccharum spp.), a major crop of tropical and sub-tropical regions (Henry and Kole, 2010), is the highest yielding crop worldwide and accounts for ~80% of the sugar (sucrose) production in the world (Nayak et al., 2014) and around 92% of that in China (Luo et al., 2012).

Sugarcane can also be used to produce ethanol as an attractive biofuel feedstock in recent years (Henry and Kole, 2010). Sugarcane belongs to the species of vegetative propagation, and most modern sugarcane varieties are only the sexual generations from wild plants, which suggests that sugarcane is of significant potential for further genetic improvement (Henry and Kole, 2010). However, it usually takes more than 10 years for traditional breeders to identify and release a new elite sugarcane variety (Berding and Roach, 1987; D'Hont et al., 1996) from a huge segregated population (Chen et al., 2011). FAPESP_BR 04/12/19 Most complete commercial sugarcane genome sequence has been assembled.

With FAPESP’s support, researchers have succeeded in mapping 99.1% of the plant’s genes, providing knowledge that will help improve its resistance to disease and increase its biomass yield for fuel or sugar production (photo:Léo Ramos Chaves / Pesquisa FAPESP). December 04, 2019 André Julião | Agência FAPESP – An international group of researchers led by Brazilian scientists has assembled the most complete genome sequence of commercial sugarcane. They mapped 373,869 genes or 99.1% of the total genome. This feat is the result of almost 20 years of research supported by FAPESP and will serve as a basis for the genetic improvement of the world’s largest crop in tonnage according to the UN Food & Agriculture Organization (FAO).

An article describing the study is published in GigaScience. Dicyt 18/10/18 Identifican genes que dotan a la caña de azúcar de una mayor resistencia contra la sequía. Científicos brasileños y belgas descubrieron cinco genes que al activarse en forma permanente en variedades transgénicas de la planta le aportan una tolerancia extra al estrés hídrico AGENCIA FAPESP/DICYT - La falta de disponibilidad de agua durante el desarrollo de la caña de azúcar constituye un tipo de estrés ambiental que genera importantes efectos negativos sobre esta planta.

LA 1ERE 24/08/18 Le génome de la canne à sucre est désormais séquencé. Le génome est le code-barre ou la carte d’identité génétique précise. La canne à sucre était la dernière grande plante à ne pas avoir le sien et les scientifiques du CIRAD (Centre de coopération internationale en recherche agronomique pour le développement) l’ont mis au grand jour. Int.J.Curr.Microbiol.App.Sci (2018) 7(7): 1594-1613 Genetic Engineering of Sugarcane for Drought and Salt Tolerant Transgenic Plants Expressing the BcZAT12 Gene. BMC PLANT BIOLOGY 10/12/18 Foreign cry1Ac gene integration and endogenous borer stress-related genes synergistically improve insect resistance in sugarcane. Transgene copies via particle bombardment are variable and random To estimate the copy number of the foreign cry1Ac gene, a quantitative TaqMan real-time PCR method was established.

This method was based on double-standard curves of cry1Ac and CYC/APRT/P4H gene, which were integrated into the multi-target recombined plasmid pG1AcAPC0229 (p1AAPC). We discovered that, for transgene copies estimation in sugarcane, the internal reference genes CYC, APRT, and P4H did not differ significantly, obtaining a high amplification efficiency between 0.95 and 1.05 [2]. The CYC gene was selected for transgene copies estimation and the transgene cry1Ac copies in transgenic sugarcane are shown in Table 1.

The proportions of different transgene cry1Ac copies in transgenic sugarcane are shown in Fig. 1. Further analysis of the cry1Ac gene copies showed that 0–1 copies/2C accounted for 18%, while 1–16 (=16) copies/2C, 17–32 copies/2C, and > 33 copies/2C accounted for 23, 41, and 18%, respectively. NATURE 08/10/18 Allele-defined genome of the autopolyploid sugarcane Saccharum spontaneum L. Genome sequencing and assembly The genome size of AP85-441 was estimated at 3.36 Gbp by flow cytometry7. PHYS_ORG 09/10/18 Success is sweet: Researchers unlock the mysteries of the sugarcane genome. For centuries, sugarcane has supplied human societies with alcohol, biofuel, building and weaving materials, and the world's most relied-upon source of sugar.

Now, researchers have extracted a sweet scientific prize from sugarcane: its massive and complex genome sequence, which may lead to the development of hardier and more productive cultivars. Tropical Plant Biology 20/02/18 Development of Transgenic Sugarcane Resistant to Sugarcane Borer. Front Plant Sci. 2017; 8: 1535. Development and Characterization of Transgenic Sugarcane with Insect Resistance and Herbicide Tolerance.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 27/03/18 Lack of Detection of Bt Sugarcane Cry1Ab and NptII DNA and Proteins in Sugarcane Processing Products Including Raw Sugar. Transgenic Research April 2018, Volume 27, Issue 2, A virus-derived short hairpin RNA confers resistance against sugarcane mosaic virus in transgenic sugarcane. Front Plant Sci. 2017 Feb 8;8:104. Field Performance of Transgenic Sugarcane Lines Resistant to Sugarcane Mosaic Virus. Introduction. BioMed Research International Volume 2015 (2015) Transgenic Sugarcane Resistant to Sorghum mosaic virus Based on Coat Protein Gene Silencing by RNA Interference.

Bragantia vol.68 no.4 Campinas 2009 Identification of cry genes from Bacillus thuringiensis effective against Sphenophorus levis, the sugar-cane borer. Identificação de genes cry de Bacillus thuringiensis no controle de Sphenophorus levis, o bicudo da cana-de-açúcar1 Identification of cry genes from Bacillus thuringiensis effective against Sphenophorus levis, the sugar-cane borer. Genomics and Applied Biology, 2017, Vol.8, No.1,1-7 Scope of Genetic Transformation in Sugarcane: A Review. SPRINGER - 2017 - Sugarcane Biotechnology: Challenges and Prospects. Sugarcane Biotechnology: Challenges and Prospects 10/08/17 CRISPR-Cas9 System as a Genome Editing Tool in Sugarcane. Plant Cell Reports January 2017, Volume 36, Transgenic sugarcane overexpressing CaneCPI-1 negatively affects the growth and development of the sugarcane weevil Sphenophorus levis. The Journal of Animal & Plant Sciences, 26(4): 2016, BIOSAFETY ASSESSMENT OF LOCALLY DEVELOPED TRANSGENIC SUGARCANE.

PLOS 28/06/17 Red rot resistant transgenic sugarcane developed through expression of β-1,3-glucanase gene. Abstract Sugarcane (Saccharum spp.) is a commercially important crop, vulnerable to fungal disease red rot caused by Colletotrichum falcatum Went. The pathogen attacks sucrose accumulating parenchyma cells of cane stalk leading to severe losses in cane yield and sugar recovery. SUGAR TECH 26/03/16 Economic Impact of Stem Borer-Resistant Genetically Modified Sugarcane in Guangxi and Yunnan Provinces of China. Alston, J.M., G.W. Norton, and P.G. Pardey. 1995. PLOS 14/07/16 Diversity of Cultivated Fungi Associated with Conventional and Transgenic Sugarcane and the Interaction between Endophytic Trichoderma virens and the Host Plant. J. ISSAAS Vol. 22, No. 1: 84-95 (2016) AGROBACTERIUM-MEDIATED TRANSFORMATION AND EXPRESSION OF BTGENE IN TRANSGENIC SUGARCANE.

International Journal of Environment and Bioenergy - 2015 - Improving Sugar Conversion and Bioethanol Yield through Transgenic Sugarcane Clone in South Africa. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - FEV 2015 - Effective selection and regeneration of transgenic sugarcane plants using positive selection system. SASA_ORG_ZA 27/10/15 Présentation : Genetically modified sugarcane and Eldana. WEEKLY TIMES 07/08/15 University of Queensland trials genetically-modified sugar cane. UNIVERSITY OF AGRICULTURE FAISALABAD (Pakistan) 01/01/12 Présentation : Impact assessment of the transgenic sugarcane over expressing antifungal proteins on endophytic and rhizospheric microorganisms. CERES INC via APHIS USDA 20/08/15 Confirmation that TRSOG103B, TRSOG102W, and TRSOG108W transgenic sugarcane are not regulated articles.

Advancements in Genetic Engineering - 2013 - Current Status of Sugarcane Transgenic: an Overview. SUGAR RESEARCH AUSTRALIA 30/04/15 What role can GM sugarcane play in sustainability. Journal of New Seeds 5(2-3):209-221 · March 2003 Expression of the Cry1Ab Protein in Genetically Modified Sugarcane for the Control of Diatraea saccharalis (Lepidoptera: Crambidae) AGRICULTURAL BIOTECHNOLOGY - 2014 - Rapid Detection of Bt Transgenic Sugarcane Using Root Tips by PCR. VIETNAMNET 23/01/16 Vietnam denies cultivation of GM sugarcane. SCIENCES 2.0 04/08/15 Miscanes: Genetically Modified Sugarcane Can Grow Farther North. ACTAHORT - 2011 - PROGRESS IN RESEARCH ON GENETICALLY MODIFIED SUGARCANE IN SOUTH AFRICA AND ASSOCIATED REGULATORY REQUIREMENTS. CATCH NEWS 04/05/16 Can GM Sugarcane really make a difference in drought-hit areas? Front. Plant Sci., 08/03/16 Cry1Ac Transgenic Sugarcane Does Not Affect the Diversity of Microbial Communities and Has No Significant Effect on Enzyme Activities in Rhizosphere Soil within One Crop Season.

PLOS 19/04/16 Transgenic Sugarcane with a cry1Ac Gene Exhibited Better Phenotypic Traits and Enhanced Resistance against Sugarcane Borer. Plant Biotechnol J. 2014 May;12(4):411-24. Field performance of transgenic sugarcane produced using Agrobacterium and biolistics methods. ISAAA - Biotechnology for Sugarcane. BioMed Research International Volume 2015 (2015) Transgenic Sugarcane Resistant to Sorghum mosaic virus Based on Coat Protein Gene Silencing by RNA Interference. EMBRAPA (Brésil) 23/05/11 Embrapa obtém primeiras plantas transgênicas de cana-de-açúcar.

Molecular Breeding, Volume 3, Number 4, August 1997 , Transgenic sugarcane plants resistant to stem borer attack. UNIVERSITY OF ILLINOIS - Programme de recherche 2002-2013 Genetically Enhanced Sorghum and Sugarcane. BMC Genomics. 2012 Nov 21;13(1):658. Genomic insights into strategies used by Xanthomonas albilineans with its reduced artillery.

BMC Genomics. 2012 Nov 21;13(1):658. Genomic insights into strategies used by Xanthomonas albilineans with its reduced artillery to spread within sugarcane xylem vessels. – guatemalt

IN VITRO CELL DEV BIOL 18/01/13 In vitro selection of transgenic sugarcane callus utilizing a plant gene encoding a mutant form. THE HINDU BUSINESS LINE 18/06/12 GM sugarcane trial planting set to take off in Brazil from Oct. THE HINDU BUSINESS LINE 26/06/12 Breeding institute develops transgenic sugarcane. STELLENBOSCH UNIVERSITY - 2009 - Thèse en ligne : Characterization of transgenic sugarcane lines with perturbed pyrophosphate: CENTRO DE TECNOLOGIA CANAVIEIRA - Is Transgenic Sugarcane a BMP ? THE HINDU 10/07/06 Indian scientists develop pest resistant transgenic sugarcane varieties. AGBIOFORUM - 2004 - Genetically Modified Sugarcane: A Case for Alternate Products. Sugar Tech (2008) 10(3) : 234-242 Biosafety and risk assessment of transgenic sugarcane plants. Sugar cane - GMO Database. OGTR (Australie) - MAI 2011 - An updated document on the biology of sugarcane has been produced to inform the risk assessment of.

Transgenic Research, Volume 20, Number 4.