SCIENTIFIC REPORTS 09/05/14 Establishment and application of a loop-mediated isothermal amplification (LAMP) system for detection of cry1Ac transgenic sugarcane. Plant materials and genomic DNA Two non-transgenic sugarcane (Saccharum spp.) cultivars, FN95-1702 and ROC22 and seventeen putative cry1Ac transgenic sugarcane clones were used as testing materials.
Among them, six clones of 19a-1, 19a-3, 19a-5, 19b-4, 20i-2 and 20i-4, were derived from the variety FN95-1702 and seven clones of 16k-1, 16k-3, 16k-5, 16d-1, 16d-2, 16d-4 and 16d-6, were derived from the variety ROC22. A-2 and A-5 were derived from GT 94-119 and the remaining two, B-2 and B-5, were derived from GT96-44. All the above plant materials are provided by the Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, China. The genomic DNA was extracted using the modified CTAB protocol40. Primer design Plasmid 1Ac0229 Plasmid 1Ac0229 which contains the cry1Ac gene was extracted from the transformed Escherichia coli DH5α strain by Plasmid Mini Kit I (Bio-tek Co., Ltd., Beijing, China) and identified by PCR.
Reaction mixture for LAMP. FRONT. PLANT SCI. 08/03/16 Detection of Bar Transgenic Sugarcane with a Rapid and Visual Loop-Mediated Isothermal Amplification Assay. Introduction Sugarcane (Saccharum spp.), a major crop of tropical and sub-tropical regions (Henry and Kole, 2010), is the highest yielding crop worldwide and accounts for ~80% of the sugar (sucrose) production in the world (Nayak et al., 2014) and around 92% of that in China (Luo et al., 2012).
Sugarcane can also be used to produce ethanol as an attractive biofuel feedstock in recent years (Henry and Kole, 2010). Sugarcane belongs to the species of vegetative propagation, and most modern sugarcane varieties are only the sexual generations from wild plants, which suggests that sugarcane is of significant potential for further genetic improvement (Henry and Kole, 2010). However, it usually takes more than 10 years for traditional breeders to identify and release a new elite sugarcane variety (Berding and Roach, 1987; D'Hont et al., 1996) from a huge segregated population (Chen et al., 2011). Materials and Methods. FAPESP_BR 04/12/19 Most complete commercial sugarcane genome sequence has been assembled. With FAPESP’s support, researchers have succeeded in mapping 99.1% of the plant’s genes, providing knowledge that will help improve its resistance to disease and increase its biomass yield for fuel or sugar production (photo:Léo Ramos Chaves / Pesquisa FAPESP).
December 04, 2019 André Julião | Agência FAPESP – An international group of researchers led by Brazilian scientists has assembled the most complete genome sequence of commercial sugarcane. They mapped 373,869 genes or 99.1% of the total genome. Dicyt 18/10/18 Identifican genes que dotan a la caña de azúcar de una mayor resistencia contra la sequía. Científicos brasileños y belgas descubrieron cinco genes que al activarse en forma permanente en variedades transgénicas de la planta le aportan una tolerancia extra al estrés hídrico AGENCIA FAPESP/DICYT - La falta de disponibilidad de agua durante el desarrollo de la caña de azúcar constituye un tipo de estrés ambiental que genera importantes efectos negativos sobre esta planta.
Con el objetivo de dotarla de una mayor resistencia al estrés hídrico, científicos de diversas instituciones de Brasil han intentado desarrollar durante los últimos años variedades de caña de azúcar mejor adaptadas las condiciones de sequía. Un grupo de investigadores del Instituto de Biología de la Universidad de Campinas (Unicamp), en colaboración con pares del Vlaams Instituut voor Biotechnologie (VIB), de Bélgica, encabezados por el investigador Dirk Inzé, ha dado un importante paso en este sentido.
“Remitimos la patente de esos genes el mes pasado. LA 1ERE 24/08/18 Le génome de la canne à sucre est désormais séquencé. Le génome est le code-barre ou la carte d’identité génétique précise.
La canne à sucre était la dernière grande plante à ne pas avoir le sien et les scientifiques du CIRAD (Centre de coopération internationale en recherche agronomique pour le développement) l’ont mis au grand jour. Une avancée scientifique importante qui devrait permettre l’amélioration variétale de la canne à sucre qui va enfin pouvoir entrer dans l’ère de la biologie moléculaire. Pour les planteurs le séquençage permet de connaître les qualités et les faiblesses des cannes, mais le danger pour les responsables de Sica Canne-Union, qui regroupe les producteurs de canne de Martinique, c'est la possibilité que cela ouvre en matière de manipulation moléculaire.
BIOMARKETINGSIGHTS 19/03/19 Australian scientist conducts gene-editing experiments on sugarcane in order to produce bio-products. Int.J.Curr.Microbiol.App.Sci (2018) 7(7): 1594-1613 Genetic Engineering of Sugarcane for Drought and Salt Tolerant Transgenic Plants Expressing the BcZAT12 Gene. BMC PLANT BIOLOGY 10/12/18 Foreign cry1Ac gene integration and endogenous borer stress-related genes synergistically improve insect resistance in sugarcane.
Transgene copies via particle bombardment are variable and random To estimate the copy number of the foreign cry1Ac gene, a quantitative TaqMan real-time PCR method was established.
This method was based on double-standard curves of cry1Ac and CYC/APRT/P4H gene, which were integrated into the multi-target recombined plasmid pG1AcAPC0229 (p1AAPC). We discovered that, for transgene copies estimation in sugarcane, the internal reference genes CYC, APRT, and P4H did not differ significantly, obtaining a high amplification efficiency between 0.95 and 1.05 . The CYC gene was selected for transgene copies estimation and the transgene cry1Ac copies in transgenic sugarcane are shown in Table 1.
The proportions of different transgene cry1Ac copies in transgenic sugarcane are shown in Fig. 1. Further analysis of the cry1Ac gene copies showed that 0–1 copies/2C accounted for 18%, while 1–16 (=16) copies/2C, 17–32 copies/2C, and > 33 copies/2C accounted for 23, 41, and 18%, respectively. NATURE 08/10/18 Allele-defined genome of the autopolyploid sugarcane Saccharum spontaneum L. Genome sequencing and assembly The genome size of AP85-441 was estimated at 3.36 Gbp by flow cytometry7.
From a BAC library of AP85-441, 35,156 BAC clones were pooled into 712 libraries (mostly of 48 BACs; Supplementary Table 1), and individual BAC pools were sequenced independently by a Hiseq 2500 with PE250 (paired-end model and 250-bp read length), yielding 267.5 Gbp of data that were assembled using three different assemblers: ALLPATHS-LG8, SPAdes9 and SOAPdenovo210, yielding a 2.56-Gbp assembly with contig N50 of 7.4 kb (Supplementary Tables 2 and 3). To reduce fragmentation, 295 Gbp of data from the Pacific Biosciences (PacBio) RS II system (Supplementary Table 4) were used for self-correction and assembly by Canu11, incorporating assembled BAC sequences and correcting and polishing with 90× Illumina paired-end sequences, yielding 3.13 Gbp with contig N50 of 45 kb (Supplementary Table 5).
A set of 4 homologous chromosomes aligned to a single sorghum chromosome. PHYS_ORG 09/10/18 Success is sweet: Researchers unlock the mysteries of the sugarcane genome. For centuries, sugarcane has supplied human societies with alcohol, biofuel, building and weaving materials, and the world's most relied-upon source of sugar.
Now, researchers have extracted a sweet scientific prize from sugarcane: its massive and complex genome sequence, which may lead to the development of hardier and more productive cultivars. Producing the comprehensive sequence required a concerted effort by over 100 scientists from 16 institutions; the work took five years and culminated in a publication in Nature Genetics. But the motivation to tackle the project arose long before. "Personally, I waited for 20 years to get this genome sequenced," said Ray Ming, a University of Illinois plant biology professor who instigated and led the sequencing effort. Tropical Plant Biology 20/02/18 Development of Transgenic Sugarcane Resistant to Sugarcane Borer.
Front Plant Sci. 2017; 8: 1535. Development and Characterization of Transgenic Sugarcane with Insect Resistance and Herbicide Tolerance. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 27/03/18 Lack of Detection of Bt Sugarcane Cry1Ab and NptII DNA and Proteins in Sugarcane Processing Products Including Raw Sugar. Transgenic Research April 2018, Volume 27, Issue 2, A virus-derived short hairpin RNA confers resistance against sugarcane mosaic virus in transgenic sugarcane. Front Plant Sci. 2017 Feb 8;8:104. Field Performance of Transgenic Sugarcane Lines Resistant to Sugarcane Mosaic Virus. Introduction Sugarcane (Saccharum officinarum L.) accounts for 90% of all sugar produced in China and 70% of sugar production worldwide.
In 2015, 10.6 million metric tons (MMT) of sugar were produced in China (Sugar: World Markets and Trade, 2015, sugar-world-markets-and-trade). Sugarcane is also an important industrial crops for sugar industries and allied industries that produce alcohol, acetic acid, butanol, paper, plywood, industrial enzymes, and animal feed (Lakshmanan et al., 2005; McQualter et al., 2005; Petrasovits et al., 2013; Raghavi et al., 2015; Zhang et al., 2015a). BioMed Research International Volume 2015 (2015) Transgenic Sugarcane Resistant to Sorghum mosaic virus Based on Coat Protein Gene Silencing by RNA Interference.
Bragantia vol.68 no.4 Campinas 2009 Identification of cry genes from Bacillus thuringiensis effective against Sphenophorus levis, the sugar-cane borer. Identificação de genes cry de Bacillus thuringiensis no controle de Sphenophorus levis, o bicudo da cana-de-açúcar1 Identification of cry genes from Bacillus thuringiensis effective against Sphenophorus levis, the sugar-cane borer Elaine Aparecida Silva CíceroI; Antonio Sergio FerraudoII; Manoel Victor Franco LemosI IUniversidade Estadual Paulista, Departamento de Biologia Aplicada à Agropecuária, E-mail: firstname.lastname@example.org, email@example.com IIUniversidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Departamento de Ciências Exatas, Via de Acesso Prof.
Paulo Donato Castellane, s/n, 14884-900 Jaboticabal (SP), E-mail: firstname.lastname@example.org, Autor correspondente. Genomics and Applied Biology, 2017, Vol.8, No.1,1-7 Scope of Genetic Transformation in Sugarcane: A Review. SPRINGER - 2017 - Sugarcane Biotechnology: Challenges and Prospects. Sugarcane Biotechnology: Challenges and Prospects 10/08/17 CRISPR-Cas9 System as a Genome Editing Tool in Sugarcane. Plant Cell Reports January 2017, Volume 36, Transgenic sugarcane overexpressing CaneCPI-1 negatively affects the growth and development of the sugarcane weevil Sphenophorus levis. The Journal of Animal & Plant Sciences, 26(4): 2016, BIOSAFETY ASSESSMENT OF LOCALLY DEVELOPED TRANSGENIC SUGARCANE.
PLOS 28/06/17 Red rot resistant transgenic sugarcane developed through expression of β-1,3-glucanase gene. Abstract Sugarcane (Saccharum spp.) is a commercially important crop, vulnerable to fungal disease red rot caused by Colletotrichum falcatum Went. The pathogen attacks sucrose accumulating parenchyma cells of cane stalk leading to severe losses in cane yield and sugar recovery.
Canne à sucre transgénique en Inde. Canne à sucre transgénique au Brésil. SUGAR TECH 26/03/16 Economic Impact of Stem Borer-Resistant Genetically Modified Sugarcane in Guangxi and Yunnan Provinces of China. Alston, J.M., G.W. Norton, and P.G. Pardey. 1995. Science under scarcity: Principled and practice for agricultural research evaluation and priority setting.
New York: Cornell University Press.Arencibia, A., R.I. Vázquez, D. MONSANTOBLOG 11/07/16 Which is More Genetically Modified: Sugarcane or Sugar Beets? PLOS 14/07/16 Diversity of Cultivated Fungi Associated with Conventional and Transgenic Sugarcane and the Interaction between Endophytic Trichoderma virens and the Host Plant. Abstract Plant-associated fungi are considered a vast source for biotechnological processes whose potential has been poorly explored. The interactions and diversity of sugarcane, one of the most important crops in Brazil, have been rarely studied, mainly concerning fungal communities and their interactions with transgenic plants.
Taking this into consideration, the purpose of this study was, based on culture dependent strategy, to determine the structure and diversity of the fungal community (root endophytes and rhizosphere) associated with two varieties of sugarcane, a non-genetically modified (SP80-1842) variety and its genetically modified counterpart (IMI-1, expressing imazapyr herbicide resistance).
For this, the sugarcane varieties were evaluated in three sampling times (3, 10 and 17 months after planting) under two crop management (weeding and herbicide treatments). Editor: Krishnendu Acharya, University of Calcutta, INDIA Copyright: © 2016 Romão-Dumaresq et al. Introduction. J. ISSAAS Vol. 22, No. 1: 84-95 (2016) AGROBACTERIUM-MEDIATED TRANSFORMATION AND EXPRESSION OF BTGENE IN TRANSGENIC SUGARCANE. International Journal of Environment and Bioenergy - 2015 - Improving Sugar Conversion and Bioethanol Yield through Transgenic Sugarcane Clone in South Africa. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - FEV 2015 - Effective selection and regeneration of transgenic sugarcane plants using positive selection system. SASA_ORG_ZA 27/10/15 Présentation : Genetically modified sugarcane and Eldana. WEEKLY TIMES 07/08/15 University of Queensland trials genetically-modified sugar cane. To use this website, cookies must be enabled in your browser. To enable cookies, follow the instructions for your browser below.
Facebook App: Open links in External Browser There is a specific issue with the Facebook in-app browser intermittently making requests to websites without cookies that had previously been set. This appears to be a defect in the browser which should be addressed soon. The simplest approach to avoid this problem is to continue to use the Facebook app but not use the in-app browser. Open the settings menu by clicking the hamburger menu in the top right Choose "App Settings" from the menu Turn on the option "Links Open Externally" (This will use the device's default browser)
UNIVERSITY OF AGRICULTURE FAISALABAD (Pakistan) 01/01/12 Présentation : Impact assessment of the transgenic sugarcane over expressing antifungal proteins on endophytic and rhizospheric microorganisms. CERES INC via APHIS USDA 20/08/15 Confirmation that TRSOG103B, TRSOG102W, and TRSOG108W transgenic sugarcane are not regulated articles. Advancements in Genetic Engineering - 2013 - Current Status of Sugarcane Transgenic: an Overview. SUGAR RESEARCH AUSTRALIA 30/04/15 What role can GM sugarcane play in sustainability. Journal of New Seeds 5(2-3):209-221 · March 2003 Expression of the Cry1Ab Protein in Genetically Modified Sugarcane for the Control of Diatraea saccharalis (Lepidoptera: Crambidae)
AGRICULTURAL BIOTECHNOLOGY - 2014 - Rapid Detection of Bt Transgenic Sugarcane Using Root Tips by PCR. Skip over navigation to the main content ProQuest Document Preview. VIETNAMNET 23/01/16 Vietnam denies cultivation of GM sugarcane. VietNamNet Bridge – The Department of Cultivation under the Ministry of Agriculture and Rural Development has rejected news that Vietnam has imported genetically modified (GM) sugarcane for cultivation. The department has written to the Vietnam Sugar and Sugarcane Association (VSSA) dismissing the news after a number of food producers asked the association to make clear whether local sugar mills used GM sugarcane or not. Circular 72/2009/TT-BNNPTNT specifies only GM corn, soybean and cotton have been test-cultivated to evaluate their impact on biodiversity and the environment in the country.
So far, the mass planting of GM corn has been approved. As of the 2013-2014 crop, Vietnam had had over 73 sugarcane varieties with 29 of them chosen by local farmers to grow on 90% of the country’s total cultivation area. Cao Anh Duong, deputy head of the Sugarcane Research Institute, said that 98% of sugarcane varieties planted in Vietnam are imported from different markets. SCIENCES 2.0 04/08/15 Miscanes: Genetically Modified Sugarcane Can Grow Farther North. U.S. farmers have long hoped to extend sugarcane's growing range northward from the Gulf coast because it substantially increase the land available for sugar and (for as long as subsidies last) biofuels. Several hybrid canes developed in the 1980s have proved hardy in cooler climes, surviving overwinter as far north as Booneville, Arkansas, but no one had tested whether the offspring of crosses between sugarcane and a hardy, cold-tolerant grass, Miscanthus - "miscanes" - actually photosynthesize, and thus continue to grow, when the thermometer dips.
Researchers now report that two miscanes perform as well as the grass species Miscanthus x giganteus at 50 degrees Fahrenheit (10 degrees Celsius), staying green and converting carbon dioxide to plant matter at a steady rate. Although the rate of photosynthesis drops at 50 degrees, it doesn't stall out altogether, as it does in sugarcane. Field studies confirmed the laboratory findings. ACTAHORT - 2011 - PROGRESS IN RESEARCH ON GENETICALLY MODIFIED SUGARCANE IN SOUTH AFRICA AND ASSOCIATED REGULATORY REQUIREMENTS. CATCH NEWS 04/05/16 Can GM Sugarcane really make a difference in drought-hit areas?
Will genetically engineered sugarcane be the panacea of drought-hit Karnataka and Maharashtra? The two states are suffering not just from shortage of drinking water, but also from economic losses from cash crops. Sugarcane, in turn, is being blamed for making the drought worse as it is a water-intensive crop. In Maharashtra, while sugarcane occupies under 10% of cultivated area, it guzzles three-fourth of all water used in agriculture. Now there are reports that the Modi government is interested in introducing a sugarcane variety that is genetically altered to grow with less water. This means that farmers won't lose money during drought and can save water too. The environment minister Prakash Javadekar has reportedly written to former agriculture minister Sharad Pawar, that the government intends to encourage field trials of the GM sugarcane developed by Indian Council of Agricultural Research's Sugarcane Breeding Institute, Coimbatore.
GM crops have always bred controversy. Front. Plant Sci., 08/03/16 Cry1Ac Transgenic Sugarcane Does Not Affect the Diversity of Microbial Communities and Has No Significant Effect on Enzyme Activities in Rhizosphere Soil within One Crop Season. PLOS 19/04/16 Transgenic Sugarcane with a cry1Ac Gene Exhibited Better Phenotypic Traits and Enhanced Resistance against Sugarcane Borer. Abstract. Plant Biotechnol J. 2014 May;12(4):411-24. Field performance of transgenic sugarcane produced using Agrobacterium and biolistics methods.
ISAAA - Biotechnology for Sugarcane. More than Just a Sugar Crop When we think of sugarcane, right away we associate it with table sugar, the most popular sweetener also chemically known as sucrose. In fact, this grass of the genus Saccharum accounts for 80% of sucrose produced worldwide, with the remaining 20% coming from sugarbeet. Each year around two billion metric tons of sugarcane stalks are crushed in sugar mills to get the sucrose juice. But there is certainly more to this crop than the sweet juice within. BioMed Research International Volume 2015 (2015) Transgenic Sugarcane Resistant to Sorghum mosaic virus Based on Coat Protein Gene Silencing by RNA Interference. EMBRAPA (Brésil) 23/05/11 Embrapa obtém primeiras plantas transgênicas de cana-de-açúcar.
Processo de transgenia pode resultar em uma planta mais tolerante à seca e minimizar problemas de escassez A Embrapa Agroenergia obteve as primeiras plantas transgênicas confirmadas de ccana-de-açúcar tolerante à seca com o gene DREB2A. As plantas foram selecionadas em laboratório e, nos próximos três meses, estarão em estágio de multiplicação in vitro para serem avaliadas em casa de vegetação. Até maio de 2012, suas características serão avaliadas. Molecular Breeding, Volume 3, Number 4, August 1997 , Transgenic sugarcane plants resistant to stem borer attack. UNIVERSITY OF ILLINOIS - Programme de recherche 2002-2013 Genetically Enhanced Sorghum and Sugarcane.
Critical Need: Biofuels offer renewable alternatives to petroleum-based fuels that reduce net greenhouse gas emissions to nearly zero. However, traditional biofuels production is limited not only by the small amount of solar energy that plants convert through photosynthesis into biological materials, but also by inefficient processes for converting these biological materials into fuels. BMC Genomics. 2012 Nov 21;13(1):658. Genomic insights into strategies used by Xanthomonas albilineans with its reduced artillery.
BMC Genomics. 2012 Nov 21;13(1):658. Genomic insights into strategies used by Xanthomonas albilineans with its reduced artillery to spread within sugarcane xylem vessels. – guatemalt
IN VITRO CELL DEV BIOL 18/01/13 In vitro selection of transgenic sugarcane callus utilizing a plant gene encoding a mutant form. THE HINDU BUSINESS LINE 18/06/12 GM sugarcane trial planting set to take off in Brazil from Oct. THE HINDU BUSINESS LINE 26/06/12 Breeding institute develops transgenic sugarcane. Coimbatore, June 26: The Sugarcane Breeding Institute has developed transgenic sugarcane incorporating gene constructs, said its Director, Dr N. STELLENBOSCH UNIVERSITY - 2009 - Thèse en ligne : Characterization of transgenic sugarcane lines with perturbed pyrophosphate: CENTRO DE TECNOLOGIA CANAVIEIRA - Is Transgenic Sugarcane a BMP ? THE HINDU 10/07/06 Indian scientists develop pest resistant transgenic sugarcane varieties.
AGBIOFORUM - 2004 - Genetically Modified Sugarcane: A Case for Alternate Products. Genetically Modified Sugarcane: A Case for Alternate Products Janet Grice, M.K. Wegener, L.M. Romanach, S. Paton, P. Bonaventura, and S. Sugar Tech (2008) 10(3) : 234-242 Biosafety and risk assessment of transgenic sugarcane plants. Sugar cane - GMO Database. Transgenic Research Volume 20, Number 4, 759-772, 2010 Transgenic sugarcane plants expressing high levels of modified cry1Ac pro.
OGTR (Australie) - MAI 2011 - An updated document on the biology of sugarcane has been produced to inform the risk assessment of. Transgenic Research, Volume 20, Number 4.