MÉDICO VETERINARIO ZOOTECNISTA - 2017 - Thèse en ligne :Detección de Hepatitis A y Norovirus (GI y GII) en muestras de agua de mar y moluscos bivalvos de las bahías de Mórrope y Sechura, mediante la técnica RT-PCR en tiempo real. EUREKALERT 27/08/19 Smartphone-Based Device for Detecting Norovirus, the 'Cruise Ship' Microbe (Video) (IMAGE) SAN DIEGO, Aug. 27, 2019 -- Made infamous by outbreaks on cruise ships, norovirus can really ruin a vacation, causing severe vomiting, diarrhea and stomach pain.
But the highly infectious virus can also strike closer to home, with water- and foodborne outbreaks occurring in municipal water systems, schools and restaurants. Today, researchers report a sensitive, portable device that can detect as few as a handful of norovirus particles in water. The researchers will present their results at the American Chemical Society (ACS) Fall 2019 National Meeting & Exposition. ACS, the world's largest scientific society, is holding the meeting here through Thursday.
It features more than 9,500 presentations on a wide range of science topics. A brand-new video on the research is available at "It only takes a very small number of norovirus particles to cause an infection in humans, so we need a really sensitive detection method," says Jeong-Yeol Yoon, Ph.D., who led the team. Title. CONTAGIONLIVE 27/08/19 Norovirus Detection Goes Mobile With New Portable Smartphone-Based Device. A new portable, smartphone-based detection system developed by investigators at the University of Arizona uses a fluorescence microscope assay to identify a miniscule amount of norovirus particles in water samples.
Because norovirus is highly contagious in low concentrations, detection is typically conducted via a polymerase chain reaction (PCR)-based method. But that requires time, a lab setting, and trained personnel. "It only takes a very small number of norovirus particles to cause an infection in humans, so we need a really sensitive detection method," Jeong-Yeol Yoon, PhD, professor of biomedical engineering at the University of Arizona, who led the team, said in a press release. "Also, scientists aren't able to culture norovirus in the lab, and available antibodies to the pathogen aren't very strong. " Investigators photographed the chip with the smartphone fluorescent microscope and ran the images through a companion app to calculate norovirus concentrations. Food Microbiology Volume 60, December 2016, Comparison and optimization of detection methods for noroviruses in frozen strawberries containing different amounts of RT-PCR inhibitors.
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Click here to view the full text on ScienceDirect. Figure 1 Norovirus-specific diagnostic in respect of beginning of the outbreak and beginning of symptoms in the index. The outbreaks were classified by the time difference, in days, between the time point of the availability of specific norovirus test result and the beginning of symptoms in the index case (grey bars) and the beginning of the outbreak (black bars). A negative value indicates that a positive norovirus test result of a symptomatic patient was available before the outbreak evolved. Figure 2. JOURNAL OF CLINICAL MICROBIOLOGY - FEV 2015 - Advances in Laboratory Methods for Detection and Typing of Norovirus.
BioMed Research International Volume 2015 (2015), Development of Enhanced Primer Sets for Detection of Norovirus. UNIVERSITE DE LIEGE - 2009 - Etude génotypique de Norovirus humains et bovins contemporains et mise au point de méthodes rapides de détection et de quantification. PLOS 23/06/14 Detecting the Norovirus Season in Sweden Using Search Engine Data – Meeting the Needs of Hospital Infection Control Teams. Abstract Norovirus outbreaks severely disrupt healthcare systems.
We evaluated whether Websök, an internet-based surveillance system using search engine data, improved norovirus surveillance and response in Sweden. We compared Websök users' characteristics with the general population, cross-correlated weekly Websök searches with laboratory notifications between 2006 and 2013, compared the time Websök and laboratory data crossed the epidemic threshold and surveyed infection control teams about their perception and use of Websök. Users of Websök were not representative of the general population. Websök correlated with laboratory data (b = 0.88-0.89) and gave an earlier signal to the onset of the norovirus season compared with laboratory-based surveillance. 17/21 (81%) infection control teams answered the survey, of which 11 (65%) believed Websök could help with infection control plans.
Editor: Vittoria Colizza, Inserm & Universite Pierre et Marie Curie, France Introduction Methods Results. Journal of Virological Methods 12/2006; Diagnosis of Norovirus outbreaks by commercial ELISA or RT-PCR. Appl Environ Microbiol. Dec 2013; 79(24): 7875–7881. Laboratory Evidence of Norwalk Virus Contamination on the Hands of Infected Individuals. International Journal of Food Microbiology Volume 160, Issue 2, 15 November 2012, Characterisation of norovirus contamination in. A Marine Institute, Rinville, Oranmore, Co.
Galway, Ireland b Microbial Ecology Laboratory, Microbiology, School of Natural Sciences, National University of Ireland, Galway, Ireland Received 18 June 2012 Revised 4 September 2012 Accepted 1 October 2012 Available online 16 October 2012 , How to Cite or Link Using DOI Abstract Norovirus (NoV) is the single most important agent of foodborne viral gastroenteritis worldwide. Highlights ► We investigated a shellfish harvest area for NoV contamination over 2 years. ► NoV concentrations in oysters demonstrated strong winter seasonality. ► NoV GI and GII showed different concentrations each year. ► NoV GI.4 was the most frequently detected genotype in oyster samples.
Keywords. Journal of Applied Microbiology - JUILLET 2005 - Detection of norovirus capsid proteins in faecal and food samples by a real tim. Page 1 ORIGINAL ARTICLEDetection of norovirus capsid proteins in faecal and foodsamples by a real time immuno-PCR methodP.
Tian and R. MandrellUnited States Department of Agriculture, Agricultural Research Service, Produce Safety and Microbiology Research Unit, Western RegionalResearch Center, Albany, CA, USAIntroductionNoroviruses (NVs) cause millions of cases of sporadicand epidemic gastrointestinal disease in the US annually,accounting for 50–67% of foodborne illness (Daniels et al.2000; Glass et al. 2001). UNIVERSITE DE LIEGE - 2009 - Thèse en ligne : Etude génotypique de norovirus humains et bovins contemporains et mise au point de.
AEM - 2008 - Norovirus detection in shellfish and approach to quantification. Food Microbiology Volume 30, Issue 2, June 2012, Detection of human norovirus in cherry tomatoes, blueberries and vegetable sala. A Shanghai Entry-Exit Inspection and Quarantine Bureau, 1208 Minsheng Road, Shanghai 200135, China b School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China c Produce Safety and Microbiology Research Unit, Western Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Albany, CA 94710, USA Received 23 September 2011 Revised 8 December 2011 Accepted 28 December 2011 Available online 11 January 2012 , How to Cite or Link Using DOI Abstract In this study, we developed a sensitive receptor-binding capture and magnetic sequestration (RBCMS) method capable of concentrating human norovirus (HuNoV) from various food samples within few hours.
Highlights Keywords Human norovirus (HuNoV) ; Receptor-binding capture and magnetic sequestration (RBCMS) ; Quantitative real time RT-PCR (qRTPCR) ; Virus recovery ; Encapsidated viral RNA Figures and tables from this article: Marine Environmental Research Volume 80, September 2012, Detection and forecasting of oyster norovirus outbreaks: Recent advance. Civil and Environmental Engineering Department, Louisiana State University, Baton Rouge, LA, USA Received 20 January 2012, Revised 1 June 2012, Accepted 12 June 2012, Available online 20 July 2012 Choose an option to locate/access this article: Check if you have access through your login credentials or your institution.
PLOS 14/11/13 Ultrasensitive Norovirus Detection Using DNA Aptasensor Technology. DNA aptamers were developed against murine norovirus (MNV) using SELEX (Systematic Evolution of Ligands by EXponential enrichment).
Nine rounds of SELEX led to the discovery of AG3, a promising aptamer with very high affinity for MNV as well as for lab-synthesized capsids of a common human norovirus (HuNoV) outbreak strain (GII.3). Using fluorescence anisotropy, AG3 was found to bind with MNV with affinity in the low picomolar range. The aptamer could cross-react with HuNoV though it was selected against MNV. As compared to a non-specific DNA control sequence, the norovirus-binding affinity of AG3 was about a million-fold higher.
In further tests, the aptamer also showed nearly a million-fold higher affinity for the noroviruses than for the feline calicivirus (FCV), a virus similar in size and structure to noroviruses. Figures. CDC EID - AOUT 2013 - Au sommaire: Emergency Department Visit Data for Rapid Detection and Monitoring of Norovirus Activity, Uni. Skip directly to local search Skip directly to A to Z list Skip directly to navigation Skip directly to site content Skip directly to page options CDC Home CDC 24/7: Saving Lives. Protecting People.