PLOS 10/07/15 Duplex Real-Time RT-PCR Assays for the Detection and Typing of Epizootic Haemorrhagic Disease Virus. Abstract Epizootic haemorrhagic disease virus (EHDV) may cause severe clinical episodes in some species of deer and sometimes in cattle.
Laboratory diagnosis provides a basis for the design and timely implementation of disease control measures. There are seven distinct EHDV serotypes, VP2 coding segment 2 being the target for serotype specificity. This paper reports the development and validation of eight duplex real-time RT-PCR assays to simultaneously amplify the EHDV target (S9 for the pan-EHDV real-time RT-PCR assay and S2 for the serotyping assays) and endogenous control gene Beta-actin. Analytical and diagnostic sensitivity and specificity, inter- and intra-assay variation and efficiency were evaluated for each assay. WIKIPEDIA - Epizootic hemorrhagic disease virus. Epizootic Hemorrhagic Disease Virus (often abbreviated EHDV) is a species of the genus Orbivirus, a member of the family Reoviridae.
It is the causative agent of Epizootic Hemorrhagic Disease, an acute, infectious, and often fatal disease of wild ruminants. In North America, the most severely affected ruminant is the white-tailed deer (Odocoileus virginianus) although it may also infect mule deer, black-tailed deer, elk, bighorn sheep, and pronghorn antelope. It is often mistakenly referred to as “Bluetongue Virus” (BTV), another Orbivirus that, like EHDV, causes the host to develop a characteristic blue tongue due to systemic hemorrhaging and lack of oxygen in the blood. Despite showing clinical similarities, these two viruses are genetically distinct. Viral classification Order: Unassigned Family: Reoviridae. WIKIPEDIA - Epizootic Hemorrhagic Disease. Epizootic hemorrhagic disease (EHD) is a hemorrhagic disease of white-tailed deer (Odocoileus virginianus) caused by an infection of a virus from the genus Orbivirus (Shope et al. 1960, Howarth et al. 2001). It is an infectious, and sometimes fatal, virus that is characterized by extensive hemorrhages, and is found throughout the United States.
History EHD was first reported in 1955 in New Jersey, killing 700 whitetails. In Alberta in 1962, EHD killed 440 whitetails, 18 mule deer, and 13 pronghorn. In a 1976 outbreak, thousands of whitetails died from EHD in Nebraska, Wyoming, Kansas, and the Dakotas, and 4000 antelope died in Wyoming. Another 1000 whitetails died from EHD in New Jersey in 1976. Geographic distribution Transmission Epizootic hemorrhagic disease is not contagious (it is not transmitted from deer to deer).
Clinical signs Deer may become infected with peracute, acute, or chronic EHD infections. Prevention and control References Texas Tech Deer Research Facility via YOUTUBE 21/05/14 EHD RESEARCH DOCUMENTARY. PAGameCommission via YOUTUBE 12/08/14 Epizootic Hemorrhagic Disease (EHD) Kansas State Veterinary Diagnostic Laboratory via YOUTUBE 21/07/16 Bluetongue and Epizootic Hemorrhagic Diseases Part1 Jim MacLachlian. Virus Genes. 2016 Aug;52(4):495-508. Molecular evolution of epizootic hemorrhagic disease viruses in North America based on historical isolates using motif fingerprints.
Research in Veterinary Science Volume 107, August 2016, Generation of virus like particles for epizootic hemorrhagic disease virus. A Dipartimento di Scienze Veterinarie, Università di Pisa, Italyb The Pirbright Institute, Pirbright, UKc Dipartimento di Biologia, Università di Pisa, Italyd Scuola Normale Superiore, Pisa, Italye Istituto Italiano di Tecnologia, Center for Nanotechnology Innovation, NEST, Pisa, Italyf NEST, Istituto Nanoscienze-CNR and Scuola Normale Superiore, Pisa, Italy Received 9 October 2015, Revised 17 May 2016, Accepted 30 May 2016, Available online 31 May 2016 Choose an option to locate/access this article: Check if you have access through your login credentials or your institution Check access Get rights and content Highlights We cloned the structural proteins of Epizootic Hemorrhagic Disease Virus serotype 6.
Two recombinant baculoviruses each expressing two viral structural proteins were generated. The presence of mRNAs and respective viral proteins was confirmed by infection of insect cells. Virus like particles were purified and visualized by Transmission Electron Microscope. Abstract Keywords. Revue Méd. Vét., 2014,165, 1-2, 2020-24 Isolation of epizootic hemorrhagic disease virus from sheep in western Turkey.
PLOS 05/08/15 Review of the 2012 Epizootic Hemorrhagic Disease Outbreak in Domestic Ruminants in the United States. Abstract An unusually large number of cases of Epizootic hemorrhagic disease (EHD) were observed in United States cattle and white-tailed deer in the summer and fall of 2012.
USDA APHIS Veterinary Services area offices were asked to report on foreign animal disease investigations and state diagnostic laboratory submissions which resulted in a diagnosis of EHD based on positive PCR results. Veterinaria Italiana 2016,52(3-4), 271-279 Epizootic haemorrhagic disease in Italy: vector competence of indigenous Culicoides species and spatial multicriteria evaluation of vulnerability. ANSES 22/05/12 Introduction de maladies animales exotiques en France : des recommandations pour améliorer la surveillance et la prévention .
Fièvre aphteuse, influenza aviaire ou encore fièvre catarrhale ovine, comme l'ont montré les crises sanitaires de la dernière décennie, il est essentiel de disposer de systèmes de lutte organisés et réactifs contre les agents pathogènes exotiques.
Afin d'identifier les maladies exotiques les plus à risque d'introduction en France et de préciser les modalités de lutte et de surveillance les plus adaptées contre ces agents, l'Anses s'est auto-saisie. Elle publie aujourd'hui les résultats de ses travaux et les recommandations qu'elle en tire. La santé des animaux est susceptible d'être menacée par divers agents pathogènes (bactéries, virus, parasites) dont certains peuvent également affecter la santé humaine (agents zoonotiques). Parmi ces agents pathogènes, un certain nombre sont présents sur le territoire métropolitain, d'autres circulent hors du territoire et sont dits "exotiques".
Dans ce contexte, l'Anses a souhaité s'autosaisir. Recommandations de l'Anses Pour en savoir plus. 785.pdf. Parasites & Vectors 2012, 5:236 Vector competence of Culicoides sonorensis (Diptera: Ceratopogonidae) to epizootic hemorrhagic d. USGS 07/09/12 Hemorrhagic Disease in Wild Ruminants. NEBRASKAGAME PARKS - 2012 - Epizootic Hemorrhagic Disease (EHD) CDC EID - FEV 2013 - Au sommaire: Epizootic Hemorrhagic Disease in Brocket Deer, Brazil. Skip directly to local search Skip directly to A to Z list Skip directly to navigation Skip directly to site content Skip directly to page options CDC Home CDC 24/7: Saving Lives.
VETERINARSKI ARHIV 74 (1), 63-83, 2004 Current status and future prospects of epizootic haemorrhagic disease of deer - a review. Le site Medreonet. EFSA 22/12/09 Scientific Opinion on Epizootic Hemorrhagic Disease. EFSA Journal 2009; 7(12):1418 [67 pp.]. doi:10.2903/j.efsa.2009.1418 Type: Opinion of the Scientific Committee/Scientific Panel On request from: European Commission Question number: EFSA-Q-2009-00503 Adopted: 02 December 2009 Published: 22 December 2009 Affiliation: European Food Safety Authority (EFSA), Parma, Italy Following a request from the European Commission, the Panel on Animal Health and Welfare was asked to deliver a scientific opinion on Epizootic Hemorrhagic Disease (EHD).
The disease has never been reported in the European Union, however in recent years outbreaks of disease caused by EHD Virus (EHDV) serotype 6 and 7, previously considered to be non-pathogenic were observed in EU neighbouring countries. Clinical signs in cattle are similar to those exhibited by bluetongue affected animals and production losses may be significant. Sheep, although susceptible to infection, do not present clinical signs. SAGIR - JANV 2012 - Lettre SAGIR N°174 La maladie hémorragique des cervidés. Veterinary Microbiology Available online 19 April 2012 Epizootic hemorrhagic disease virus serotype 7 in European cattle and she. Abstract Epizootic hemorrhagic disease virus (EHDV), an arthropod-borne orbivirus (family Reoviridae), is an emerging pathogen of wild and domestic ruminants that is closely related to bluetongue virus (BTV).
The present study examines the outcome of an experimental EHDV-7 infection of Holstein cattle and East Frisian sheep. Apart from naïve animals that had not been exposed to BTV, it included animals that had been experimentally infected with either BTV-6 or BTV-8 two months earlier.