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Maladie de Marek

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BMC Veterinary Research 24/08/20 Detection of p53 mutation and serum monitoring alert caused by Marek’s disease virus in poultry. Natural infection of MDV in chickens Seven 160-day-old egg-laying hens were obtained from a local flock.

BMC Veterinary Research 24/08/20 Detection of p53 mutation and serum monitoring alert caused by Marek’s disease virus in poultry

These hens were confirmed to have acquired MDV infection naturally with the absence of other common viral diseases by PCR detection. Their serums were collected for the detection of the P53 antigen and antibody. Their tissue samples, including liver, spleen, pancreas, and bursa of Fabricius, were collected and fixed with 4% formaldehyde for 48 h before histopathological and immunohistochemical studies. Experimental infection of chickens with MDV The number of experimental animal was referred to the number of clinical samples. Histopathology and Immunohistochemistry The fixed liver, spleen, pancreas, and bursa of Fabricius were dehydrated, waxed, and cut into 3 µm slices, followed by Haematoxylin and eosin (H-E) staining for a histopathology examination. Total RNA isolation and reverse transcription Enzyme-linked immunosorbent assay (ELISA) for P53 and P53 antibody Statistical analysis.

AMERICAN ASSOCIATION OF AVIAN PATHOLOGISTS - JUNE 2013 - Comparison of Loop-Mediated Isothermal Amplification and PCR for the Detection and Differentiation of Marek's Disease Virus Serotypes 1, 2, and 3. Veterinary Microbiology Volume 236, September 2019, Spatial and temporal variation of Marek’s disease virus and infectious laryngotracheitis virus genome in dust samples following live vaccination of layer flocks. Abel et al., 1999 U.

Veterinary Microbiology Volume 236, September 2019, Spatial and temporal variation of Marek’s disease virus and infectious laryngotracheitis virus genome in dust samples following live vaccination of layer flocks

Abel, R. Schosser, J. SüssEstimating the prevalence of infectious agents using pooled samples: biometrical considerations Zentralblatt für Bakteriologie, 289 (1999), pp. 550-563 Ahaduzzaman et al., 2019. UFRGS_BR - 2019 - Thèse en ligne: Doença de Marek em poedeiras comerciais vacinadas : relato de caso e análise metagenômica. TRANSBOUNDARY AND EMERGING DISEASES 14/08/19 Marek's disease viruses circulating in commercial poultry in Italy in the years 2015–2018 are closely related by their meq gene phylogeny. Marek's disease (MD) is a lymphoproliferative disease important to the poultry industry worldwide; it is caused by Gallid alphaherpesvirus 2 (GaHV‐2).

TRANSBOUNDARY AND EMERGING DISEASES 14/08/19 Marek's disease viruses circulating in commercial poultry in Italy in the years 2015–2018 are closely related by their meq gene phylogeny

The virulence of GaHV‐2 isolates has shifted over the years from mild to virulent, very virulent and very virulent +. Nowadays the disease is controlled by vaccination, but field strains of increased virulence are emerging worldwide. Economic losses due to MD are mostly associated with its acute form, characterized by visceral lymphomas.

The present study aimed to molecularly classify a group of 13 GaHV‐2 strains detected in vaccinated Italian commercial chicken flocks during acute MD outbreaks, and to scrutinize the ability of predicting GaHV‐2 virulence, according to the meq gene sequence. The full‐length meq genes were amplified, and the obtained amino acid (aa) sequences were analysed, focusing mainly on the number of stretches of four proline molecules (PPPP) within the transactivation domain. Rev.MVZ Cordoba vol.24 no.1 Córdoba Jan/Apr. 2019 Challenges in the diagnosis and control of Marek's disease virus in Colombia. Marek's Disease (MD) is a global neuropathic and lymphoproliferative disease in chickens (Gallus domesticus) caused by a highly contagious oncogenic alphaherpesvirinae [1,2.

Rev.MVZ Cordoba vol.24 no.1 Córdoba Jan/Apr. 2019 Challenges in the diagnosis and control of Marek's disease virus in Colombia

Its genomic organization and molecular structure are similar to the human Herpes Simplex Virus (HSV) of the alphaherpesvirinae subfamily [1,3]. However there are some unique features that differentiate Marek's Disease Virus (MDV) from other Alpha-herpesvirus: it establishes latency in lymphocytes (T-cells), it is strictly cell associated, encodes for an oncogene (Meq), and is able to induce CD4+ T cell lymphomas [3,4]. The disease is characterized by immunosuppression, infiltration of infected lymphocytes into peripheral nerves and visceral organs (liver, kidney, spleen, gonads, heart and proventriculus) and ultimately the formation of metastatic T-cell Lymphomas [5,6]. Affected unvaccinated and susceptible chickens will usually succumb to the disease, resulting in high morbidity and mortality [7].

Qf. Poultry Science Available online 22 January 2020 Attenuation of a recombinant Marek's disease virus lacking the meq oncogene and evaluation on its immune efficacy against Marek's disease virus. Abdul-Careem et al., 2006.

Poultry Science Available online 22 January 2020 Attenuation of a recombinant Marek's disease virus lacking the meq oncogene and evaluation on its immune efficacy against Marek's disease virus

Int. J. Mol. Sci. 2019, 20(13), 3150 Marek’s Disease Virus Infection Induced Mitochondria Changes in Chickens. Mitochondria are crucial cellular organelles in eukaryotes and participate in many cell processes including immune response, growth development, and tumorigenesis.

Int. J. Mol. Sci. 2019, 20(13), 3150 Marek’s Disease Virus Infection Induced Mitochondria Changes in Chickens

Marek’s disease (MD), caused by an avian alpha-herpesvirus Marek’s disease virus (MDV), is characterized with lymphomas and immunosuppression. In this research, we hypothesize that mitochondria may play roles in response to MDV infection. To test it, mitochondrial DNA (mtDNA) abundance and gene expression in immune organs were examined in two well-defined and highly inbred lines of chickens, the MD-susceptible line 72 and the MD-resistant line 63. We found that mitochondrial DNA contents decreased significantly at the transformation phase in spleen of the MD-susceptible line 72 birds in contrast to the MD-resistant line 63. The mtDNA-genes and the nucleus-genes relevant to mtDNA maintenance and transcription, however, were significantly up-regulated. ►▼ Show Figures Figure 1 ►▼ Show Figures. FRONT. MICROBIOL. 13/12/19 Increased Horizontal Transmission of Recombinant Marek’s Disease Virus Due to Reticuloendotheliosis Virus Long Terminal Repeat Is the Major Competitive Advantage of the Virus Being a Prevalent Strain.

Introduction Marek’s disease (MD), induced by the Marek’s disease virus (MDV), is a contagious lymphoproliferative disease of poultry (Churchill and Biggs, 1967).

FRONT. MICROBIOL. 13/12/19 Increased Horizontal Transmission of Recombinant Marek’s Disease Virus Due to Reticuloendotheliosis Virus Long Terminal Repeat Is the Major Competitive Advantage of the Virus Being a Prevalent Strain

GX0101 is the first natural recombinant MDV field strain isolated from birds showing tumors in China (Cui et al., 2010). We constructed an infectious bacterial artificial chromosome (BAC) clone of GX0101, which showed characteristics similar to those of the parental virus in replication and pathogenicity (Sun et al., 2009). The complete genome of GX0101 was sequenced and analyzed using the GX0101 BAC clone (Su et al., 2012, 2013).

It contains a 538-bp reticuloendotheliosis virus (REV) long terminal repeat (LTR) inserted between nucleotide bases “C” and “A” numbered 153,175–153,176 (Md5 strain) or 154,507–154,508 (RB1B strain). Epidemics Volume 27, June 2019, Managing Marek’s disease in the egg industry. 1.

Epidemics Volume 27, June 2019, Managing Marek’s disease in the egg industry

Introduction The industrialization of farming empowers farmers to keep pace with the ever-increasing demands of consumers, but it also creates a situation highly conducive to pathogen evolution as a result of cramped living conditions and shorter rearing periods. A primary example of this is Marek’s disease virus (MDV), which is a disease of poultry that has evolved from a relatively harmless paralytic syndrome into a highly virulent pathogen (Witter, 1997) as a result of industrialization (Atkins et al., 2012; Rozins and Day, 2017).

Braz. J. Poult. Sci. vol.21 no.1 Campinas 2019 Epub May 09, 2019 An Overview on Marek’s Disease Virus Evolution and Evidence for Increased Virulence in Brazil. AMERICAN ASSOCIATION OF AVIAN PATHOLOGISTS - JUNE 2006 - Detection and Characterization of Avian Leukosis Virus in Marek's Disease Vaccines. J. VET. MED. SCI. 22/12/08 Microarray Analysis of Host Immune Responses to Marek’s Disease Virus Infection in Vaccinated Chickens. AVIAN PATHOLOGY 03/07/18 Spotlight on avian pathology: Marek’s disease. PLOS 29/01/18 Attenuation of a very virulent Marek's disease herpesvirus (MDV) by codon pair bias deoptimization. Acta virologica 59: 156 – 165, 2015 Pathotyping of recent Indian field isolates of Marek's disease virus serotype 1.

BIORXIV 04/10/18 Managing Marek’s disease in the egg industry. J Vet Sci. 2018 May; 19(3): 375–383. Marek's disease vaccine activates chicken macrophages. BMC VETERINARY RESEARCH 03/04/18 First molecular detection and characterization of Marek’s disease virus in red-crowned cranes (Grus japonensis): a case report. In August 2015, reduced feed consumption was observed in a 2-month-old red-crowned crane (crane A) that was bred and incubated in Hongshan Forest Zoo in Nanjing, China.

BMC VETERINARY RESEARCH 03/04/18 First molecular detection and characterization of Marek’s disease virus in red-crowned cranes (Grus japonensis): a case report

After segregation and palliative treatment, it exhibited astasia and then died. Subcutaneous palpation examination revealed many diffuse soybean-sized nodules (Table 1). Red-crowned crane B showed obvious swelling of its left knee joint. After antibiotic treatment, although it showed an improved appetite and a decrease in symptoms, the crane died unexpectedly ten days later (Table 1). The dead cranes were not immunized with the MDV vaccine. Clinical necropsy revealed diffuse yellow-white nodules from millet to soybean size in the skin, muscle, trachea, liver, gizzard, and heart in both cranes (Fig. 1a-f). Representative tissue samples were collected during necropsy from the liver, spleen, kidney, muscle, heart, feather follicles, and skin, and these were used for pathological and virological investigations (Table 1).

UNIV_KIEV_UA - 2013 - IDENTIFICATION OF МAREK'S DISEASE IN POULTRY FARMS OF UKRAINE AND ESTABLISHMENT THE FORMS OF DISEASE.

Maladie de Marek et vaccination

JOURNAL OF CLINICAL EPIGENETICS - 2017 - Genomic Variation between Genetic Lines of White Leghorns Differed in Resistance to Marek’s Disease. Indian Vet. J., December 2016, 93 (12) : 35 - 37 Marek’s Disease Outbreak in Commercial Layer Flocks. Arq. Bras. Med. Vet. Zootec. vol.68 no.6 Belo Horizonte Nov./Dec. 2016 Visceral Marek's disease in white-peafowl (Pavo cristatus) Veterinary Medicine.

Arq. Bras. Med. Vet. Zootec. vol.68 no.6 Belo Horizonte Nov./Dec. 2016 Visceral Marek's disease in white-peafowl (Pavo cristatus)

Virus Research Volume 235, 2 May 2017, Comparison of different sites in recombinant Marek’s disease virus for the expression of green fluorescent protein. <div pearltreesdevid="PTD140" role="alert" class="alert-message-container"><div pearltreesdevid="PTD141" aria-hidden="true" class="alert-message-body"><span pearltreesdevid="PTD142" style="display: inline-block;" class="Icon IconAlert"><svg pearltreesDevId="PTD143" style="width: 100%; height: 100%;" width="24" height="24" focusable="false" tabindex="-1" fill="currentColor"><path pearltreesDevId="PTD144" fill="#f80" d="M11.84 4.63c-.77.05-1.42.6-1.74 1.27-1.95 3.38-3.9 6.75-5.85 10.13-.48.83-.24 1.99.53 2.56.7.6 1.66.36 2.5.41 3.63 0 7.27.01 10.9-.01 1.13-.07 2.04-1.28 1.76-2.39-.1-.58-.56-1.02-.81-1.55-1.85-3.21-3.69-6.43-5.55-9.64-.42-.52-1.06-.83-1.74-.79z"></path><path pearltreesDevId="PTD145" d="M11 8h2v5h-2zM11 14h2v2h-2z"></path></svg></span><!

-- react-text: 58 -->JavaScript is disabled on your browser. Please enable JavaScript to use all the features on this page. <! -- /react-text --></div></div> Highlights We constructed five recombinant MDVs containing eGFP gene at various sites. Veterinary Immunology and Immunopathology Volume 180, 1 November 2016, Marek’s disease virus immunosuppression alters host cellular responses and immune gene expression in the skin of infected chickens.

<div pearltreesdevid="PTD138" role="alert" class="alert-message-container"><div pearltreesdevid="PTD139" aria-hidden="true" class="alert-message-body"><span pearltreesdevid="PTD140" style="display: inline-block;" class="Icon IconAlert"><svg pearltreesDevId="PTD141" style="width: 100%; height: 100%;" width="24" height="24" focusable="false" tabindex="-1" fill="currentColor"><path pearltreesDevId="PTD142" fill="#f80" d="M11.84 4.63c-.77.05-1.42.6-1.74 1.27-1.95 3.38-3.9 6.75-5.85 10.13-.48.83-.24 1.99.53 2.56.7.6 1.66.36 2.5.41 3.63 0 7.27.01 10.9-.01 1.13-.07 2.04-1.28 1.76-2.39-.1-.58-.56-1.02-.81-1.55-1.85-3.21-3.69-6.43-5.55-9.64-.42-.52-1.06-.83-1.74-.79z"></path><path pearltreesDevId="PTD143" d="M11 8h2v5h-2zM11 14h2v2h-2z"></path></svg></span><!

-- react-text: 58 -->JavaScript is disabled on your browser. Please enable JavaScript to use all the features on this page. <! JOURNAL AVIAN PATHOLOGY 24/04/17 Evaluation of factors influencing the development of late Marek’s disease virus-induced immunosuppression: virus pathotype and host sex. WebImagesPlus… AAAPJOURNALS - JUNE 2017 - Industry-Wide Surveillance of Marek's Disease Virus on Commercial Poultry Farms. Poult Sci. 2017 Jun 1;96(6):1598-1608. Molecular characterization of Marek's disease virus in a poultry layer farm from Colombia.

AVIAN PATHOLOGY - 2001 - The use of serotype 1- and serotype 3-specific polymerase chain reaction for the detection of Marek's disease virus in chickens. WebImagesPlus… Connexion Recherche avancée Scholar Recherche avancée Articles contenant. VETERINARY WORLD - JUNE 2016 - Diversity of Meq gene from clinical Marek’s disease virus infection in Saudi Arabia.

Indian Vet. J., September 2015, 92 (9) : 78 - 80 Co-Occurrence of Marek’s Disease and Inclusion Body Hepatitis in a Broiler Parent Stock. INDIAN VET. J. - MAY 2015 - Marek’s Disease in an Amazon Parrot (Amazona sp.) POULTRY SITE 17/11/15 Marek’s Disease in Small Flocks. Featured Articles Marek’s Disease in Small Flocks 17 November 2015 Christine Heinrichs is a freelance agricultural journalist based the US. She's the author of How to Raise Chickens and How to Raise Poultry, and a regular contributor to a variety of poultry publications. This article from Christine Heinrichs explains the symptoms of Marek's Disease in poultry and how to prevent it occurring in small flocks. AVIAN PATHOLOGY 10/08/15 The epizootiology of Marek's disease 2. The effect of supply flock, rearing, house and production house on the incidence of Marek's disease.

USDA - AOUT 2014 - Developing Tools To Fight Marek’s Disease. New testsand vaccines are making it easier to detect viruses in chickens and protect them from the cancer-like diseases some of them cause. One of the latest tools developed by scientists at the Agricultural Research Service’s Avian Disease and Oncology Laboratory (ADOL) in East Lansing, Michigan, is a modified polymerase chain reaction (PCR) test to detect unique genetic sequences of both Marek’s disease and avian reticuloendotheliosis viruses in various tissues from chickens. Marek’s disease is caused by a highly contagious herpes virus that primarily affects young adult chickens.

It causes severe production losses and usually death and is spread from bird to bird by contaminated dust and dander in chicken houses. Avian reticuloendotheliosis, a retrovirus, infects a broader host range—chickens, turkeys, ducks, geese, and quail. It can also cause tumors, production losses, and death in affected birds. IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) - AVRIL 2014 - Detection of Marek’s disease virus meq gene in Feather follicle by Loop-mediated Isothermal Amplification.

Veterinary Microbiology Volume 177, Issues 1–2, 15 May 2015, Pathogenic characteristics of Marek's disease virus field strains prevalent in China and the effectiveness of existing vaccines against them. A Northeast Agricultural University, Harbin 150030, PR Chinab Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China Received 6 May 2014, Revised 17 December 2014, Accepted 21 December 2014, Available online 2 January 2015 Choose an option to locate/access this article: Check if you have access through your login credentials or your institution Check access.

Vet Res. 2014; 45(1): 36. Marek’s disease virus and skin interactions. BMC 23/07/15 Outbreak of Marek’s disease in a vaccinated broiler breeding flock during its peak egg-laying period in China. Marek’s disease is one of the most significant infectious diseases in poultry. In birds, it can result in tumours or immunosuppression, making the birds more sensitive to other pathogens. Although MD vaccines have been widely used in China for a long time, failures of MD vaccination occur frequently on some poultry farms. The improper selection of a vaccine could be the cause of vaccine failure [5].

Birds are also more susceptible to MDV mutants that have emerged in regions with more-virulent strains than to previous MDV isolates. BMC 23/07/15 Outbreak of Marek’s disease in a vaccinated broiler breeding flock during its peak egg-laying period in China. UNIVERSITE de François Rabelais TOURS - Thèse 2000-2001 Expression et caractérisation de quatre protéines du virus de la maladie.

UNIVERSITE de François Rabelais TOURS - Thèse 2000-2001 Expression et caractérisation de quatre protéines du virus de la maladie de Marek homologues aux protéines majeures de tégument VP22, VP16, VP13/14 et VP11/12 du virus HSV-1. – guatemalt

OIE - Code sanitaire 2010 - Maladie de Marek. Avian Diseases 57(2s1):483-490. 2013 Current Status of Marek's Disease in the United States and Worldwide Based on a Questionnai. Revue Méd. Vét., 2013, 164, 3, 106-111 Etude lésionnelle de la maladie de Marek chez le poulet de chair et chez les reproducteur.

Revue Méd. Vét., 2013, 164, 3, 106-111 Etude lésionnelle de la maladie de Marek chez le poulet de chair et chez les reproducteurs dans l’Est algérien – guatemalt

POULTRYSITE 11/11/13 Progress on Control of Marek's Disease Discussed at US Veterinary Convention.