JOURNAL OF VIROLOGICAL METHODS 18/10/05 Quantitative detection of a marine fish iridovirus isolated from large yellow croaker, Pseudosciaena crocea, using a molecular beacon. JOURNAL OF VETERINARY MEDICAL SCIENCE - 2018 - Assessment of fish iridoviruses using a novel cell line GS-1, derived from the spleen of orange-spotted grouper Epinephelus coioides (Hamilton) and susceptible to ranavirus and megalocytivirus. Abstract A new cell line (GS-1) was developed from the spleen tissue of the orange-spotted grouper, Epinephelus coioides applied for viral infection studies of fish ranavirus and megalocytivirus.
The cells proficiently multiplied in Leibovitz’s L-15 medium supplemented with 10% fetal bovine serum at temperatures between 20°C and 32°C. Morphologically, the cell line comprised fibroblast-like cells, and this was confirmed by immunostaining with vimentin, fibronectin, and desmin antibodies. The optimal temperature for grouper iridovirus (GIV) and infectious spleen and kidney necrosis virus (ISKNV) proliferation in GS-1 cells was 25°C, and the highest titer of GIV was 108.4 TCID50/ml, and the highest titer of ISKNV was 105.2 TCID50/ml.
Electron micrographs showed that the mean diameter of GIV virions was 180−220 nm, which was larger than ISKNV virions (160−200 nm). FRONT. MICROBIOL. 26/06/20 Singapore Grouper Iridovirus (SGIV) Inhibited Autophagy for Efficient Viral Replication. Introduction Singapore grouper iridovirus (SGIV) is a novel marine fish virus isolated from diseased groupers (Qin et al., 2001).
The clinical symptoms of SGIV-challenged fishes are hemorrhage and enlargement of the spleen. This lethal pathogen has caused considerable economic damage in groupers, with more than 90% mortality (Qin et al., 2003). To date, the morphogenesis, biochemical pathology, genome, transcriptome, proteomics, and entry mechanisms of SGIV have been systematically studied (Qin et al., 2003; Song et al., 2004; Wang et al., 2014). SGIV is an icosahedral virus with diameter of 154–176 nm, and it belongs to the genus Ranavirus and family Iridoviridae (Qin et al., 2001, 2003). Autophagy is a conserved catabolic process that maintains cellular homeostasis by sequestering damaged organelles or misfolded proteins in the autophagosome and fusing with lysosomes for degradation and recycling (Xie and Klionsky, 2007; Klionsky et al., 2011).
Materials and Methods Virus Infection. AIP_CONFERENCE - 2019 - Studies on iridovirus infection among grouper fish (Epinephelus sp.) cultured in Seribu Islands, Indonesia. AQUACULTURE REPORTS - AOUT 2017 - The presence of Vibrionaceae, Betanodavirus and Iridovirus in marine cage-cultured fish: Role of fish size, water physicochemical parameters and relationships among the pathogens.
AFS (Annual Fisheries Statistic)Annual Fisheries Statistic Department of Fisheries, Ministry of Agriculture & Agro-Based Industry, Malaysia (2014) Albert and Ransangan, 2013 V.
Albert, J. RansanganEffect of water temperature on susceptibility of culture marine fish species to vibriosis Int. Amal et al., 2010a M.N.A. Online J. Amal et al., 2010b M.N.A. J. Amal et al., 2015 M.N.A. Aquac. Arulampalam et al., 1998 P. Aquac. Asrazitah et al., 2014 A.R. Inter. Binesh, 2013. FISH PATHOLOGY - 2016 - A Novel Method for Detection of Fish Iridovirus in Groupers using Nano-Magnetic Particles. Journal of Fish Diseases 33(2):95-122 · February 2010 Iridovirus infections in fish – critical review with emphasis on ranaviruses. Journal of Agricultural Science and Technology B 6 (2016) 121-128 Detection of Fish Disease Caused by Iridovirus on Grouper (Epinephelus sp.) and Pomfret Star (Trachinotus blochii) with Co-agglutination Method in Tanjungpinang, Indonesia. WORLD AQUATIC VETERINARY MEDICAL ASSOCIATION 15/09/16 Vidéo : Iridovirus Infections of Fish. B-1027: Iridovirus Infections of Fish Presenter: Dr.
Paul Hick Date: September 15, 2016 Time: 3.00 GMT This event has already taken place but you still have options please read on.... Duration: 1.25 hours Career level: Veterinarians, Veterinary Scientists, Veterinary Students CEPD Credits: 1.5 Hours of CEPD A recording of this event Please find below a recording of this event. Thanks for signing up as a guest member...A confirmation email has been sent to the address that you used during registration.Please click on the account activation link in the confirmation email.
More about this event This webinar will cover Iridoviridae that includes three genera of viruses relevant to fish health: Lymphocystivirus, Ranavirus and Megalocytivirus.