BMC Microbiology 03/05/17 Simultaneous detection of chikungunya virus, dengue virus and human pathogenic Leptospira genomes using a multiplex TaqMan® assay. The aim of our study was to implement a syndromic approach based upon the use of a multiplex real time RT-PCR assay to facilitate rapid diagnosis of dengue-like syndromes in Reunion Island.
This syndromic approach was in phase with regional health agency preoccupations because of the recurrent occurrences of leptospirosis but also taking into account the unprecedented major CHIKV outbreak in 2005–2006 and the regular number of clustered cases of DENV . On the one hand, the use of a syndromic panel is appealing for economic reasons. It is cost saving due to the use of lower amount of enzyme than in simplex reactions. The multiplex assay requires less handle-time and it is subjected to lower risk of undesirable cross-reaction contaminations. On the other hand, multiplex assays have been criticized for a loss of sensitivity compared to their simplex counterparts. PJMHS - OCT/DEC 2016 - Comparative Seropositivity Against Leptospirosis Between Rice Cultivators and Non Rice Cultivators. NATIONAL ACADEMY OF AGRARIAN SCIENCES OF UKRAINE - 2016 - JOURNAL FOR VETERINARY MEDICINE, BIOTECHNOLOGY AND BIOSAFETY THE VALIDATION OF ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DIAGNOSIS LEPTOSPIROSIS AMONG DOGS, PIGS AND CATTLE.
BMC 24/08/16 Utility of modified Faine’s criteria in diagnosis of leptospirosis. The present study evaluated the utility of modified Faine’s criteria (with amendment) 2012 for the diagnosis of leptospirosis in Sri Lanka.
Diagnosis of leptospirosis in the rural hospitals with minimal facilities is a major challenge. This study aimed at facilitating diagnosis using a simple method utilizing clinical, epidemiological and basic laboratory investigations available in rural hospitals. Leptospirosis mimics a number of other important infections due to the nonspecific nature of the clinical presentation and therefore can be misdiagnosed frequently. Further, confirmatory laboratory tests for leptospira such as MAT and PCR are only available in reference laboratories where the facilities for leptospira culture and molecular biology are available.
Therefore, it is essential that feasible and reliable diagnostic criteria based on clinical symptoms and basic laboratory tests be identified and their diagnostic utility evaluated for resource poor settings such as Sri Lanka. PLOS 03/08/16 An Optimized Method for Quantification of Pathogenic Leptospira in Environmental Water Samples. Abstract Leptospirosis is a zoonotic disease usually acquired by contact with water contaminated with urine of infected animals.
However, few molecular methods have been used to monitor or quantify pathogenic Leptospira in environmental water samples. Here we optimized a DNA extraction method for the quantification of leptospires using a previously described Taqman-based qPCR method targeting lipL32, a gene unique to and highly conserved in pathogenic Leptospira. QIAamp DNA mini, MO BIO PowerWater DNA and PowerSoil DNA Isolation kits were evaluated to extract DNA from sewage, pond, river and ultrapure water samples spiked with leptospires. PLOS 12/11/15 Leptospira and Bats: Story of an Emerging Friendship. Citation: Dietrich M, Mühldorfer K, Tortosa P, Markotter W (2015) Leptospira and Bats: Story of an Emerging Friendship.
PLoS Pathog 11(11): e1005176. doi:10.1371/journal.ppat.1005176 Editor: William E. Goldman, The University of North Carolina at Chapel Hill, UNITED STATES Published: November 12, 2015 Copyright: © 2015 Dietrich et al. Funding: This work was made possible by funding from the National Research Foundation, South Africa (NRF – N00595) to MD. Competing interests: The authors have declared that no competing interests exist.
A growing number of recent studies have highlighted bats as a reservoir for Leptospira bacteria, pointing out the potential role of bats in the epidemiology of the most widespread zoonotic disease in the world . PLOS 07/11/14 Sensitive Real-Time PCR Detection of Pathogenic Leptospira spp. and a Comparison of Nucleic Acid Amplification Methods for the Diagnosis of Leptospirosis. Abstract Background Bacteria of the genus Leptospira, the causative agents of leptospirosis, are categorized into pathogenic and non-pathogenic species.
However, the benefit of using a clinical diagnostic that is specific for pathogenic species remains unclear. In this study, we present the development of a real-time PCR (rtPCR) for the detection of pathogenic Leptospira (the pathogenic rtPCR), and we perform a comparison of the pathogenic rtPCR with a published assay that detects all Leptospira species [the undifferentiated febrile illness (UFI) assay] and a reference 16S Leptospira rtPCR, which was originally designed to detect pathogenic species. Methodology/Principal Findings For the pathogenic rtPCR, a new hydrolysis probe was designed for use with primers from the UFI assay, which targets the 16S gene.
Conclusions/Significance The pathogenic rtPCR displayed similar sensitivity to the UFI assay when testing clinical specimens with no difference in specificity. Introduction. Identification of immunodominant antigens in canine leptospirosis by Multi-Antigen Print ImmunoAssay (MAPIA) Lung India. 2012 Oct-Dec; 29(4): 347–353. Pulmonary manifestations of leptospirosis. THIRD INTERNATIONAL EPIZOOTIOLOGY DAYS & XV SERBIAN EPIZOOTIOLOGY DAYS PROCEEDINGS - MAI 2013 - Au sommaire:LEPTOSPIROSIS IN PIG.
International Journal of Microbiological Research (IJMR) Volume 4 Number (2), 2013 Global Epidemiological Overview of Leptospiro. Braz. J. Microbiol. vol.44 no.1 São Paulo 2013 Characterization of a virulent Leptospira interrogans strain isolated from an aba. Characterization of a virulent Leptospira interrogans strain isolated from an abandoned swimming pool Karine M.
ForsterI; Daiane D. HartwigI; Fabiana K. SeixasI; Alan J.A. McBrideI,II; Leonardo G. INúcleo de Biotecnologia, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, RS, Brazil IIInstituto Gonçalo Moniz, Fundação Oswaldo Cruz, Ministério da Saúde, Salvador, BA, Brazil IIICentro de Controle de Zoonoses, Universidade Federal de Pelotas, Pelotas, RS, Brazil Send correspondence to. PLOS 07/11/14 Sensitive Real-Time PCR Detection of Pathogenic Leptospira spp. and a Comparison of Nucleic Acid Amplification Met. Background Bacteria of the genus Leptospira, the causative agents of leptospirosis, are categorized into pathogenic and non-pathogenic species.
However, the benefit of using a clinical diagnostic that is specific for pathogenic species remains unclear. In this study, we present the development of a real-time PCR (rtPCR) for the detection of pathogenic Leptospira (the pathogenic rtPCR), and we perform a comparison of the pathogenic rtPCR with a published assay that detects all Leptospira species [the undifferentiated febrile illness (UFI) assay] and a reference 16S Leptospira rtPCR, which was originally designed to detect pathogenic species.
Methodology/Principal Findings For the pathogenic rtPCR, a new hydrolysis probe was designed for use with primers from the UFI assay, which targets the 16S gene. Conclusions/Significance The pathogenic rtPCR displayed similar sensitivity to the UFI assay when testing clinical specimens with no difference in specificity. Figures Introduction Methods Ethics. J Emerg Trauma Shock. 2008 Jan-Jun; 1(1): 21–33. Leptospirosis: The “mysterious” mimic.
INTECH - AVRIL 2012 - Zoonosis. Au sommaire: Insights into Leptospirosis, a Neglected Disease. Edited by Jacob Lorenzo-Morales, ISBN 978-953-51-0479-7, 448 pages, Publisher: InTech, Chapters published April 04, 2012 under CC BY 3.0 licenseDOI: 10.5772/2125 Edited Volume Zoonotic diseases are mainly caused by bacterial, viral or parasitic agents although "unconventional agents" such as prions could also be involved in causing zoonotic diseases.
Many of the zoonotic diseases are a public health concern but also affect the production of food of animal origin thus they could cause problems in international trade of animal-origin goods. A major factor contributing to the emergence of new zoonotic pathogens in human populations is increased contact between humans and animals. This book provides an insight on zoonosis and both authors and the editor hope that the work compiled in it would help to raise awareness and interest in this field. INTECH - MARS 2012 - Au sommaire: Leptospirosis: Epidemiologic Factors, Pathophysiological and Immunopathogenic. Edited by Alfonso J.
Rodriguez-Morales, ISBN 978-953-51-0274-8, 576 pages, Publisher: InTech, Chapters published March 16, 2012 under CC BY 3.0 licenseDOI: 10.5772/1335 Edited Volume Tropical Medicine has emerged and remained as an important discipline for the study of diseases endemic in the tropic, particularly those of infectious etiology. Emergence and reemergence of many tropical pathologies have recently aroused the interest of many fields of the study of tropical medicine, even including new infectious agents. Then evidence-based information in the field and regular updates are necessary. Current Topics in Tropical Medicine presents an updated information on multiple diseases and conditions of interest in the field.
UNIVERSIDAD ZARAGOZA via EFSA - 2012 - Inventory of available data and data sources and proposal for data collection on vector-b. Veterinary World - Vol.1 No.6 June 2008 Leptospirosis : a re-emerging disease. Leptospirosis - Wikipedia. Lipat ning kikilalanan deng metung kareng pekamaleparan pangakalat kareng zoonosis, metung yang malagad mung sakit ibat kareng bacteria cumpara kareng aliwa kareng tau.
Keraklan, mikakalat ya iti kapamilatan ning danum a mirinan imi da reng animal, a mitatagkil kareng e kinayap a sugat o aspak king balat, mata o mucous membrane. Kilual da reng mangapaling lugal, makapanaun la reng casu ning leptospirosis, at lalto la reti neng Agosto anggang Setiembre/Febrero anggang Marzo. Dalerayan Jump up ↑ James, William D.; Berger, Timothy G.; et al. (2006). UNIVERSIDADE DE SAO PAULO - 2008 - Patogênese da síndrome pulmonar hemorrágica na leptospirose humana. Tese de Doutorado. OMS - 2009 - The Global Burden of Leptospirosis. The Global Burden of Leptospirosis What do we know? Leptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira. It is most commonly spread via water contaminated with urine from infected animals, but contaminated food or soil can also act as vehicles for the disease. The main animal reservoirs are rodents, livestock and dogs. Disease in humans can vary from mild flu-like illness to serious disease. Braz J Infect Dis vol.11 no.1 Salvador Feb. 2007 Au sommaire: Pathology and pathophysiology of pulmonary manifestations in lepto.
J. Venom. Anim. Toxins incl. Trop. Dis vol.17 no.2 Botucatu 2011 Interactions between environment, wild animals and human leptos. Interactions between environment, wild animals and human leptospirosis Ullmann LSI; Langoni HII IPostgraduate Program in Veterinary Medicine, School of Veterinary Medicine and Animal Husbandry, São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, São Paulo State, Brazil IIDepartment of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Husbandry, São Paulo State University (UNESP - Univ Estadual Paulista), Botucatu, São Paulo State, Brazil Correspondence to Leptospirosis, a worldwide distributed zoononis caused by bacteria of the genus Leptospira (antigenically classified into serovars), may be direct or indirectly transmitted through infected urine or environment.
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY - 2006 - Leptospirosis: Current status and future trends. Leptospirosis: Current status and future trends RA Hartskeerl Department of Biomedical Research, Royal Tropical Institute (KIT), Meibergdreef 39, 1105 AZ Amsterdam, Netherlands Correspondence Address:R A HartskeerlDepartment of Biomedical Research, Royal Tropical Institute (KIT), Meibergdreef 39, 1105 AZ Amsterdam Netherlands Leptospirosis is particularly prevalent in warm humid countries. Based on global data collected by International Leptospirosis Society (ILS) surveys, there are currently 300,000 to 500,000 severe cases of leptospirosis annually. However, this number presents probably a strong underestimation and the true extent of leptospirosis remains unknown because worldwide surveillance is lacking.
Sheep a major source of leptospirosis infection. The changing profile of victims of leptospirosis and the high number of cases amongst Hawke’s Bay meat workers have prompted a co-operative study into the disease between Massey University’s EpiCentre and meat company PPCS. The research was carried out in February and March at the PPCS Takapau plant. Thirteen of 15 patients admitted to the ICU at Hawke’s Bay Hospital suffering from leptospirosis from 1999-2005 were employed as meat workers or inspectors. Blood samples and interviews were conducted with 242 volunteers. J Vet Med Sci. 2009 Sep;71(9):1191-9. Nationwide survey of leptospira antibodies in dogs in Japan: results from microscopic aggl. Veterinary World - Volume 2 No.7 July 2009 Leptospriosis in sheep and its diagnosis. Shivraj, Dr.; Venkatesha, M.D.; Sanjukta, Rajkumari; Sripad, K.; SanjeevKumar, Dr.; Chandranail, B.M. and Renukaprasad, C. (2009) Leptospriosis in sheep and its diagnosis.Veterinary World, 2 (7). pp. 263-264.
ISSN 0972-8988 Full text available as: The present study was carried out to screen or detect the leptospirosis in the migratory flocks of sheep in Karnataka. A total of 60 blood, tissue and urine samples were collected from the migratory sheep flock in the area of Shimogga and Belgaum districts of Karnataka with clinical manifestation. The samples were subjected to screening for leptospirosis by Dark Field Microscopy (DFM), Polymerase chain reaction (PCR), isolation and identification studies. Archive Staff Only: edit this record. INTERDISCIPLINARY PERSPECTIVES ON INFECTIOUS DISEASES - 2012 - Serological Evidence of Exposure to Leptospira spp. in Veterinary.