Vaccine Volume 35, Issue 29, 22 June 2017 Deinococcus Mn2+-peptide complex: A novel approach to alphavirus vaccine development. Open Access Highlights V3526 and CHIK 181/25 were inactivated at 20 kGy or higher doses of gamma-radiation.
Viral surface proteins were protected from radiation-induced degradation in the presence of MnDpPi. The presence of MnDpPi did not interfere with degradation of the viral genome during irradiation. Immunization with V3526 irradiated in the presence of MnDpPi resulted in higher survival and total antibody response in vivo. Abstract. BMC Microbiology 03/05/17 Simultaneous detection of chikungunya virus, dengue virus and human pathogenic Leptospira genomes using a multiplex TaqMan® assay. The aim of our study was to implement a syndromic approach based upon the use of a multiplex real time RT-PCR assay to facilitate rapid diagnosis of dengue-like syndromes in Reunion Island.
This syndromic approach was in phase with regional health agency preoccupations because of the recurrent occurrences of leptospirosis but also taking into account the unprecedented major CHIKV outbreak in 2005–2006 and the regular number of clustered cases of DENV . On the one hand, the use of a syndromic panel is appealing for economic reasons. It is cost saving due to the use of lower amount of enzyme than in simplex reactions. The multiplex assay requires less handle-time and it is subjected to lower risk of undesirable cross-reaction contaminations.
On the other hand, multiplex assays have been criticized for a loss of sensitivity compared to their simplex counterparts. Dealing with multiplex set up assays, we paid a critical attention to sensitivity. NATURE SCIENTIFIC REPORT 20/03/17 A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses.
Assay speed and sensitivity We aimed to develop a rapid and reliable QUASR RT-LAMP assay for ZIKV, while relying on previously reported RT-LAMP assays for CHIKV and DENV17,19,22.
We first set about identifying an optimal primer set for ZIKV, using the LAMP-compatible SYTO intercalating dyes to perform real-time monitoring. We then adapted the optimal primer set to the target-specific QUASR endpoint detection technique. The mechanism and chemistry of QUASR-based RT-LAMP detection techniques has previously been described in detail22. At least one recent publication describes a different RT-LAMP assay for ZIKV utilizing leuco crystal violet indicator13. We prepared a ZIKV RNA standard from cultured ZIKV. Am J Trop Med Hyg. 2016 Dec 5. pii: 16-0516. Detection of Wolbachia in Aedes albopictus and Their Effects on Chikungunya Virus. Asian Pacific Journal of Tropical Medicine Volume 9, Issue 10, October 2016 Chikungunya infection: A potential re-emerging global threat.
Abstract Infectious diseases are indeed a lifelong threat to everyone irrespective of age, sex, lifestyle and socio-economic status.
The infectious diseases have persisted among the prominent causes of death globally. Recently, re-emergence of Chikungunya viral infection harmed many in Asian and African countries. Chikungunya was considered as a major threat in developing and under-developed countries; the recent epidemiological outbreak of Chikungunya in La Reunion urges the global researchers to develop effective vaccine against this viral disease. PLoS Negl Trop Dis. 2016 Apr 28;10(4):e0004677. The wMel Strain of Wolbachia Reduces Transmission of Chikungunya Virus in Aedes aegypti.
Abstract Background New approaches to preventing chikungunya virus (CHIKV) are needed because current methods are limited to controlling mosquito populations, and they have not prevented the invasion of this virus into new locales, nor have they been sufficient to control the virus upon arrival.
A promising candidate for arbovirus control and prevention relies on the introduction of the intracellular bacterium Wolbachia into Aedes aegypti mosquitoes. This primarily has been proposed as a tool to control dengue virus (DENV) transmission; however, evidence suggests Wolbachia infections confer protection for Ae. aegypti against CHIKV. Although this approach holds much promise for limiting virus transmission, at present our understanding of the ability of CHIKV to infect, disseminate, and be transmitted by wMel-infected Ae. aegypti currently being used at Wolbachia release sites is limited.
Methodology/Principal Findings Conclusions/Significance Author Summary Editor: Philip M. Annals of Clinical Microbiology and Antimicrobials 22/07/16 ChikDenMaZika Syndrome: the challenge of diagnosing arboviral infections in the midst of concurrent epidemics. Single-Reaction Multiplex Reverse Transcription PCR for Detection of Zika, Chikungunya, and Dengue Viruses - Volume 22, Number 7—July 2016. Jesse J.
Waggoner, Lionel Gresh, Alisha Mohamed-Hadley, Gabriela Ballesteros, Maria Jose Vargas Davila, Yolanda Tellez, Malaya K. Sahoo, Angel Balmaseda, Eva Harris, and Benjamin A. Pinsky ( EUROSURVEILLANCE 19/05/16 Global distribution and environmental suitability for chikungunya virus, 1952 to 2015. BMC 03/03/16 Co-distribution and co-infection of chikungunya and dengue viruses. We are witnessing a rapid expansion in the geographical extent of chikungunya which mirrors that of dengue as described by Gubler in the 1990s .
This has come about partly through the increased opportunity for pathogen and vector spread that has resulted from globalisation , and the multifaceted effects on infectious diseases of a growing human population with resultant environmental changes . Perhaps equally important, however, is the reporting bias that has obscured the public health impact of this pathogen, from its discovery until quite recently; CHIKV was first isolated in 1953 from the serum of a suspected dengue patient  and its conflation with dengue has persisted. SCIENTIFIC DATA 07/07/15 The global compendium of Aedes aegypti and Ae. albopictus occurrence. PLOS 26/06/14 Arrival of Chikungunya Virus in the New World: Prospects for Spread and Impact on Public Health. Citation: Weaver SC (2014) Arrival of Chikungunya Virus in the New World: Prospects for Spread and Impact on Public Health.
PLoS Negl Trop Dis 8(6): e2921. doi:10.1371/journal.pntd.0002921 Published: June 26, 2014 Copyright: © 2014 Scott C. Weaver. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: The author's research on chikungunya virus is supported by NIH grants AI093491, AI057156 and AI069145. Competing interests: I have read the journal's policy and have the following conflicts: The author is an inventor of a patent for alphavirus attenuation. For the first time in modern scientific history, chikungunya virus has established its mosquito-human transmission cycle in the Americas. Figure 1. CDC EID - AOUT 2014 - Preparedness for Threat of Chikungunya in the Pacific.
Adam Roth ( , Damian Hoy, Paul F.
Horwood, Berry Ropa, Thane Hancock, Laurent Guillaumot, Keith Rickart, Pascal Frison, Boris Pavlin, and Yvan Souares Author affiliations: Secretariat of the Pacific Community, Noumea, New Caledonia (A. Roth, D. Hoy, P. Suggested citation for this article Abstract Chikungunya virus (CHIKV) caused significant outbreaks of illness during 2005–2007 in the Indian Ocean region. CDC EID - Volume 20, Number 8—August 2014 PDF Version[PDF - 7.58 MB - 1 67 pages]. Au sommaire notamment: Transcontinental Movement of Asian Genotype Chikungunya Virus. Suggested citation for this article To the Editor: Chikungunya virus (CHIKV), a mosquito–transmitted virus (family Togaviridae, genus Alphavirus), was first isolated >60 years ago in Africa and is responsible for epidemics of acute polyarthralgia.
During CHIKV epidemics, the transmission cycle is from humans to mosquitoes, with no intervening amplifying host, and the virus can rapidly disseminate, infecting large numbers of persons. Epidemics have been described in Africa, the Middle East, Europe, India, and Southeast Asia. On the basis of detailed clinical descriptions of the disease, chikungunya fever, it appears that CHIKV caused epidemics in the Caribbean (St. Thomas, US Virgin Islands) and the southeastern coastal United States during the early 19th century (1).
Genetic studies show that the virus has evolved into 3 distinct genotypes: West African, East/Central/South African (ECSA), and Asian (2). CDC EID - Volume 20, Number 8—August 2014 PDF Version[PDF - 7.58 MB - 167 pages]. Au sommaire notamment: Movement of Chikungunya Virus into the Western Hemisphere. Author affiliation: Centers for Disease Control and Prevention, Fort Collins, Colorado, USA Suggested citation for this article Chikungunya virus (CHIKV) is an alphavirus transmitted in an urban epidemic cycle by the mosquitoes Aedes aegypti and Ae. albopictus. CHIKV outbreaks are characterized by rapid spread and infection rates as high as 75%; 72%–93% of infected persons become symptomatic. The disease manifests as acute fever and potentially debilitating polyarthralgia.
CDC EID - Volume 20, Number 12—December 2014. Au sommaire: Evaluation of Commercially Available Serologic Diagnostic Tests for Chikungunya Virus. Author affiliations: French Armed Forces Biomedical Research Institute (IRBA), Marseille, France (C.M. Prat, O. Flusin, B. Viruses 2014, 6(11), 4628-4663 Chikungunya Virus–Vector Interactions. 1 Center for Vectorborne Diseases, School of Veterinary Medicine, University of California, Davis, CA 95616, USA 2 Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA 3 Department of Virology, Arboviruses and Insect Vectors, Institut Pasteur, 25-28 rue du Dr.
Roux, 75724 Paris cedex 15, France 4 Institute for Human Infections and Immunity, Center for Tropical Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA * Author to whom correspondence should be addressed. Received: 3 September 2014 / Revised: 10 November 2014 / Accepted: 10 November 2014 / Published: 24 November 2014. SCIENCE NEWS 02/06/15 Chikungunya is on the move - The virus has found a new hemisphere and might get a new latitude. A crippling virus has slipped its bonds in Africa and Asia and is invading whole new continents faster than people canlearn to pronounce its name.
In one decade, chikungunya (chihk-uhn-GUHN-yuh) fever has gone from an obscure tropical ailment to an international threat, causing more than 3 million infections worldwide. The virus has established itself in Latin America and may now have the wherewithal to inflict its particular brand of misery in cooler climates. Chikungunya rarely kills its victims, but it can bring a world of hurt. It comes on like the flu — fever, chills, headache, aching joints — and typically lingers for a week. Many patients later develop severe joint pain that can recur for months or years. Chikungunya Virus–Vector Interactions. NEW MICROBIOLOGICA, 36, 211-227, 2013 Chikungunya virus infection: an overview. Molecular Ecology (2012) 21, 2297–2309 Chikungunya virus impacts the diversity of symbiotic bacteria in mosquito vector. Journal of Ophthalmic Inflammation and Infection 2013, 3:35 Chikungunya and the eye: a review.
INTECH - MARS 2012 - Au sommaire: The Re-Emergence of an Old Disease: Chikungunya Fever. Edited by Alfonso J. World J Virol 2012 February 12; 1(1): 11-22 Emerging and re-emerging viruses: A global challenge illustrated by Chikungunya viru. The few examples mentioned above illustrate the major development in the relations between pathogen, vector, animal and human target within ecosystems that have never changed so quickly. PLOS 24/01/12 Climate Teleconnections and Recent Patterns of Human and Animal Disease Outbreaks. Abstract Background Recent clusters of outbreaks of mosquito-borne diseases (Rift Valley fever and chikungunya) in Africa and parts of the Indian Ocean islands illustrate how interannual climate variability influences the changing risk patterns of disease outbreaks. Although Rift Valley fever outbreaks have been known to follow periods of above-normal rainfall, the timing of the outbreak events has largely been unknown.
Journal of Vector Ecology Volume 36, Issue 1, pages 108–116, June 2011 Surveillance of the chikungunya vector Aedes albopictus ( Aedes albopictus (Skuse, 1894) was probably introduced into Europe through the tire trade, and was first detected in Albania in 1979 ( Adhami and Murati 1987 ), then in Italy ( Sabatini et al. 1990 , Dalla Pozza and Majori 1992 ), France ( Schaffner and Karch 2000 ), Serbia and Montenegro ( Petrić et al. 2001 ), Switzerland ( Flacio et al. 2004 ), Belgium ( Schaffner et al. 2004 ), Greece ( Samanidou et al. 2005 ), Spain ( Aranda et al. 2006 ), Croatia ( Klobučar et al. 2006 ), Slovenia, and Bosnia and Herzegovina ( Scholte and Schaffner 2007 ). In the Emilia-Romagna region of northern Italy, the first detection of Ae. albopictus occurred in Bologna and dates back to 1994 (Carrieri, unpublished data), then the species spread all over the region. Since 2006, the Public Health Service of the Emilia-Romagna region has developed a monitoring system for Ae. albopictus with the aim of obtaining information about its temporal evolution ( Angelini 2006 ).
AuthorMapper. Chikungunya: A Potentially Emerging Epidemic? Indian Journal of Medical Microbiology: Table of Contents.